pycom04g03970

trigalactosyldiacylglycerol 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007397
Physical Location & Seq
Forward (+)
3548142 .. 3552124
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03970.1

Sequence Viewer

Length: 1137 bp
ATGATAGGGAATTCGTTGGTCCAAGTATCCACATGCCCCACGGTGTTGTCTTTGTCACGCAGTTCTTCAAATAGTTTGCCTTATCTTCCTTCCAGATCACCAACAAAAGCAACCCGGATAAGAGCTGCATCGGCAGAGACAGGACATAGCCAACAACCCTCCTCTTCTTCTGAGAAAAAGAATCCACTTGCAGTTGTTTTGGATGCCCCTCGGACCATTTGGAGGCAAACATTACGACCGTTGAGTGACTTTGGGTTTGGTCGTAAAAGTATATGGGAAGGTGGGGTAGGATTGTTTCTAGTTTCAGGTGCAGTTCTCGTTGCACTCAGTTTGGCTTGGATACGGGGTTATCAGTTGAGGTCTAAATTCAGGAAGTACTTTGCTGTGTTTGAGTTCACCGAAGCTTGCGGAATATCCACTGGAACACCAGTGAGGATTAGAGGGGTCACTGTAGGCAATGTTATCCGTGTTAACTCTTCCTTGGAAAGTATTGAAGCTGTTGTTGAGGTTGAAGATGATAAAACTGTCATACCTCGAAATGCACTGATTGAGGTAAACCAGTCGGGTCTTCTCATGGAAACTAGAATTGACATTACACCTAAAGACCCACTTCCGAATATTTCAGTTGGGCCTCTTCATCCTGAATGTGGCAAAGAAGGTCTGATTGTATGCGATAGGCAAAAGATGAGAGGACATCAAGGTGTGAGTTTAGATGCATTGGTAGGTATATTTACTCGTCTTGGACGTGAAGTGGAGGAGATTGGTATAGCTAACACTTATTCGCTTGCTGAACGAGTTGCTTCCATGATAGAAGAGGCAAAGCCACTGCTTATAAAGATCCAAGCCATGGCTGAAGATGTTCAACCTTTGCTGGCTGAGTTTCGTGATAGTGGGTTGCTGAAGGAAGTTGATAGTTTGACCAGAAGTGTTACGCAAGCCTCGGAAGATTTGAGAAGGGTGCATTCAGCTGTTATGACTCCTGAGAATACAGAACTGATTCAGAAATCCGTATACAGTTTGATTTTCACCTTGAAGAATATTGAGAATATAAGCTCTGATATTCTGGGATTCACGGGTGACGAGGCTACAAGAAAAAATTTGAAGTTACTGATCAAGTCCCTCAGCAGGCTACTGTGA

Protein Analysis

379

Amino Acids

41.38

Weight (kDa)

8.82

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaD PF02470 125 - 200 4.8e-15 MlaD protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 847
AccI GTMKAC 1 cut(s) 1011
AciI CCGC 1 cut(s) 408
AclWI GGATC 1 cut(s) 832
AcsI RAATTY 3 cut(s) 10, 365, 1096
AcuI CTGAAG 2 cut(s) 873, 920
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 4 cut(s) 222, 647, 847, 1125
AgsI TTSAA 6 cut(s) 69, 494, 512, 863, 1033, 1102
AjiI CACGTC 1 cut(s) 746
AluBI AGCT 6 cut(s) 125, 404, 497, 770, 968, 1053
AluI AGCT 6 cut(s) 125, 404, 497, 770, 968, 1053
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 832
AoxI GGCC 1 cut(s) 629
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 3 cut(s) 10, 365, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp700I GAANNNNTTC 2 cut(s) 858, 996
AspS9I GGNCC 3 cut(s) 19, 213, 629
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 4 cut(s) 90, 388, 1018, 1088
AvaII GGWCC 2 cut(s) 19, 213
BbsI GAAGAC 1 cut(s) 560
BbvCI CCTCAGC 1 cut(s) 1121
BbvI GCAGC 1 cut(s) 112
BciVI GTATCC 2 cut(s) 37, 333
BclI TGATCA 1 cut(s) 1110
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 299, 582
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 2 cut(s) 37, 333
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 115
Bme18I GGWCC 2 cut(s) 19, 213
BmgBI CACGTC 1 cut(s) 746
BmgT120I GGNCC 3 cut(s) 19, 213, 629
BmrFI CCNGG 1 cut(s) 115
BmsI GCATC 3 cut(s) 137, 193, 703
BpiI GAAGAC 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 1121
BpuMI CCSGG 1 cut(s) 115
BsaJI CCNNGG 5 cut(s) 39, 209, 480, 846, 939
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 647, 847, 1125
Bse1I ACTGG 3 cut(s) 424, 428, 559
Bse3DI GCAATG 1 cut(s) 463
BseDI CCNNGG 5 cut(s) 39, 209, 480, 846, 939
BseGI GGATG 2 cut(s) 208, 637
BseLI CCNNNNNNNGG 4 cut(s) 222, 647, 847, 1125
BseMI GCAATG 1 cut(s) 463
BseMII CTCAG 5 cut(s) 162, 340, 867, 972, 1135
BseNI ACTGG 3 cut(s) 424, 428, 559
BseRI GAGGAG 2 cut(s) 151, 770
BseXI GCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 330
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 1 cut(s) 631
BsiEI CGRYCG 1 cut(s) 239
BsiSI CCGG 1 cut(s) 115
BslFI GGGAC 1 cut(s) 1102
BslI CCNNNNNNNGG 4 cut(s) 222, 647, 847, 1125
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 1102
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 1 cut(s) 631
Bsp143I GATC 3 cut(s) 95, 837, 1110
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 1 cut(s) 408
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 5 cut(s) 163, 339, 868, 973, 1134
BspPI GGATC 1 cut(s) 832
BsrDI GCAATG 1 cut(s) 463
BsrI ACTGG 3 cut(s) 424, 428, 559
BssECI CCNNGG 5 cut(s) 39, 209, 480, 846, 939
BssMI GATC 3 cut(s) 95, 837, 1110
BssNAI GTATAC 1 cut(s) 1012
BssT1I CCWWGG 2 cut(s) 480, 846
Bst1107I GTATAC 1 cut(s) 1012
Bst4CI ACNGT 6 cut(s) 43, 240, 451, 526, 1016, 1134
Bst6I CTCTTC 4 cut(s) 169, 481, 639, 807
BstC8I GCNNGC 5 cut(s) 406, 786, 873, 936, 1127
BstDEI CTNAG 5 cut(s) 171, 326, 876, 981, 1121
BstDSI CCRYGG 2 cut(s) 39, 846
BstF5I GGATG 2 cut(s) 208, 637
BstKTI GATC 3 cut(s) 98, 840, 1113
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 95, 837, 1110
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 113
BstSFI CTRYAG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 1 cut(s) 837
BstYI RGATCY 1 cut(s) 837
BstZ17I GTATAC 1 cut(s) 1012
BsuI GTATCC 2 cut(s) 37, 333
BsuRI GGCC 1 cut(s) 631
BtgI CCRYGG 2 cut(s) 39, 846
BtrI CACGTC 1 cut(s) 746
BtsCI GGATG 2 cut(s) 208, 637
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 5 cut(s) 417, 435, 447, 542, 824
Cac8I GCNNGC 5 cut(s) 406, 786, 873, 936, 1127
Cfr13I GGNCC 3 cut(s) 19, 213, 629
Csp6I GTAC 1 cut(s) 376
CviAII CATG 4 cut(s) 33, 574, 805, 847
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 5 cut(s) 171, 326, 876, 981, 1121
DpnI GATC 3 cut(s) 97, 839, 1112
DpnII GATC 3 cut(s) 95, 837, 1110
Eam1104I CTCTTC 4 cut(s) 169, 481, 639, 807
EarI CTCTTC 4 cut(s) 169, 481, 639, 807
Eco130I CCWWGG 2 cut(s) 480, 846
Eco47I GGWCC 2 cut(s) 19, 213
Eco57I CTGAAG 2 cut(s) 873, 920
EcoRI GAATTC 1 cut(s) 10
EcoT14I CCWWGG 2 cut(s) 480, 846
EcoT22I ATGCAT 1 cut(s) 718
ErhI CCWWGG 2 cut(s) 480, 846
FaeI CATG 4 cut(s) 36, 577, 808, 850
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 1102
FatI CATG 4 cut(s) 32, 573, 804, 846
FbaI TGATCA 1 cut(s) 1110
FblI GTMKAC 1 cut(s) 1011
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 2 cut(s) 215, 624
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 2 cut(s) 299, 582
GluI GCNGC 1 cut(s) 126
HaeIII GGCC 1 cut(s) 631
HapII CCGG 1 cut(s) 115
Hin1II CATG 4 cut(s) 36, 577, 808, 850
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 4 cut(s) 181, 976, 997, 1068
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 1 cut(s) 115
HphI GGTGA 4 cut(s) 90, 388, 1018, 1088
Hpy166II GTNNAC 4 cut(s) 396, 472, 556, 1012
Hpy188I TCNGA 7 cut(s) 172, 213, 615, 663, 943, 1002, 1057
Hpy188III TCNNGA 5 cut(s) 93, 370, 641, 884, 980
Hpy8I GTNNAC 4 cut(s) 396, 472, 556, 1012
HpyAV CCTTC 5 cut(s) 99, 272, 650, 895, 948
HpyCH4III ACNGT 6 cut(s) 43, 240, 451, 526, 1016, 1134
HpyCH4IV ACGT 1 cut(s) 745
HpyCH4V TGCA 7 cut(s) 128, 191, 311, 323, 542, 716, 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 5 cut(s) 171, 326, 876, 981, 1121
HpySE526I ACGT 1 cut(s) 745
Hsp92II CATG 4 cut(s) 36, 577, 808, 850
Ksp22I TGATCA 1 cut(s) 1110
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 3 cut(s) 95, 837, 1110
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 3 cut(s) 137, 193, 703
MaeI CTAG 2 cut(s) 299, 582
MaeII ACGT 1 cut(s) 745
MaeIII GTNAC 6 cut(s) 54, 245, 445, 928, 1076, 1104
MalI GATC 3 cut(s) 97, 839, 1112
MboI GATC 3 cut(s) 95, 837, 1110
MflI RGATCY 1 cut(s) 837
MluCI AATT 4 cut(s) 10, 365, 585, 1096
MlyI GAGTC 1 cut(s) 970
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 2 cut(s) 858, 996
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 961
MwoI GCNNNNNNNGC 1 cut(s) 131
NciI CCSGG 1 cut(s) 115
NcoI CCATGG 1 cut(s) 846
NdeII GATC 3 cut(s) 95, 837, 1110
NlaIII CATG 4 cut(s) 36, 577, 808, 850
NmuCI GTSAC 4 cut(s) 54, 245, 445, 1076
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 2 cut(s) 858, 996
PfeI GAWTC 3 cut(s) 181, 997, 1068
PflMI CCANNNNNTGG 1 cut(s) 847
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 1 cut(s) 970
PpsI GAGTC 1 cut(s) 970
PsiI TTATAA 1 cut(s) 833
PspPI GGNCC 3 cut(s) 19, 213, 629
PsuI RGATCY 1 cut(s) 837
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 1 cut(s) 699
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 3 cut(s) 95, 837, 1110
Sau96I GGNCC 3 cut(s) 19, 213, 629
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 1 cut(s) 970
ScrFI CCNGG 1 cut(s) 115
SfaNI GCATC 3 cut(s) 137, 193, 703
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 19, 213
SmiMI CAYNNNNRTG 1 cut(s) 699
Sse9I AATT 4 cut(s) 10, 365, 585, 1096
SsiI CCGC 1 cut(s) 408
SspI AATATT 2 cut(s) 619, 1039
SspMI CTAG 2 cut(s) 299, 582
StyD4I CCNGG 1 cut(s) 113
StyI CCWWGG 2 cut(s) 480, 846
TaaI ACNGT 6 cut(s) 43, 240, 451, 526, 1016, 1134
TaiI ACGT 1 cut(s) 748
TaqI TCGA 1 cut(s) 535
TasI AATT 4 cut(s) 10, 365, 585, 1096
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 3 cut(s) 181, 997, 1068
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 5 cut(s) 424, 435, 454, 549, 831
TseFI GTSAC 4 cut(s) 54, 245, 445, 1076
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 4 cut(s) 54, 245, 445, 1076
TspDTI ATGAA 1 cut(s) 626
TspGWI ACGGA 2 cut(s) 455, 997
TspRI CASTG 5 cut(s) 424, 435, 454, 549, 831
Van91I CCANNNNNTGG 1 cut(s) 847
VpaK11BI GGWCC 2 cut(s) 19, 213
XapI RAATTY 3 cut(s) 10, 365, 1096
XceI RCATGY 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 1011
XmnI GAANNNNTTC 2 cut(s) 858, 996
XspI CTAG 2 cut(s) 299, 582
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.