Rw2G021970

trigalactosyldiacylglycerol 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007397
Physical Location & Seq
Forward (+)
30677971 .. 30682987
5017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021970.1

Sequence Viewer

Length: 1134 bp
ATGATAGGAAATTCTTTGGCCCAAGTCTCAACATGCCCCACAATGTTGTCTTTGTCACGTTTTTCTTCAAATGGCTTGCCTTATCTCCCTCCAAGGTCAACAAAATTAACCCAGATAAGGGCTACATCTGCTAATACTGGACACAGTCAACCACAGTCCTCTTCTAAGAGAGAAAGGAATATTCTTGCAGTTGTTTTGGATGCTCCTCGTACTATTTGGAGGCAAACATTACGACCATTGAGTGATTTTGGGTTTGGTCGTAGAAGCATATGGGAAGGTGGGGTAGGGCTGTTTCTAGTCTCAGGGGCAGTTCTCCTTGCACTCACTTTGGCTTGGTTGAGGGGTTATCAATTGCGGTCTAGATTCAGGAAGTACTTAGCTGTTTTTGAGTTTACTCAAGCTTGTGGAATATCCACTGGAACGCCAGTGCGGATCAGAGGGGTCACTGTTGGAAATGTTATCCGTGTTAACTCTTCCTTGAAAAGTATCGAAGCTGTTGTTGAGGTTGAAGATGATAAAACTATGATACCTCGAAATTCACTGATTGAGGTAAATCAGTCAGGTCTTCTCATGGAAACAAGAATTGACATTACACCTAGAGATCCAATTCCTAATATTTCAGTTGGGCCTCTTCATCCTGAATGCGACAAAGAAGGTGTAATTGTATGTGATAGGCAAAAGATGAAGGGATTTCAAGGGGTAAGTTTAGATGAATTGGTAGGCATATTCACCCGTCTGGGACGTGAAGTGGAGGAGATTGGTATTGCGAACACCTATTCACTTGCTGAGCGTGTTGCTTCTGTTATAGAAGAGGCAAAGCCACTGCTGAAAAAGATCCAAGCCATGGCTGAAGATGTTCAACCTTTGTTGGCTGAGGTTCATGATAGTGGGCTGCTAAAGGAAGTTGAGACTTTGACCAAAAGCCTTGCACAAGCCTCGGAAGATTTGAGGAGAGTGCACTCATCTATTATGACTCCTGAGAATACAGAACTGATTCAGAAGTCCATATACACTTTGATTTTCACCATGAAGAACATTGAGAATATAAGCTCTGATATTTTGGGATTCACGGGTGATGAGGCTACAAGAAAAAATTTGAAGTTACTCATCAAGTCTCTCAGCAGGCTATTATGA

Protein Analysis

377

Amino Acids

41.66

Weight (kDa)

9.12

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaD PF02470 124 - 199 9e-16 MlaD protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 844
AciI CCGC 2 cut(s) 355, 430
AclWI GGATC 3 cut(s) 440, 596, 829
AcsI RAATTY 3 cut(s) 10, 535, 1093
AcuI CTGAAG 1 cut(s) 870
AfaI GTAC 2 cut(s) 211, 374
AfiI CCNNNNNNNGG 3 cut(s) 117, 118, 844
AgsI TTSAA 6 cut(s) 69, 481, 509, 695, 860, 1099
AjiI CACGTC 1 cut(s) 743
AluBI AGCT 4 cut(s) 380, 401, 494, 1050
AluI AGCT 4 cut(s) 380, 401, 494, 1050
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 4 cut(s) 31, 304, 902, 1119
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 3 cut(s) 440, 596, 829
AoxI GGCC 2 cut(s) 18, 626
ApaLI GTGCAC 1 cut(s) 956
ApeKI GCWGC 1 cut(s) 892
ApoI RAATTY 3 cut(s) 10, 535, 1093
Asp700I GAANNNNTTC 2 cut(s) 855, 993
AspS9I GGNCC 2 cut(s) 19, 626
AsuHPI GGTGA 3 cut(s) 721, 1015, 1085
BaeGI GKGCMC 1 cut(s) 960
BarI GAAGNNNNNNTAC 2 cut(s) 992, 1024
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 960
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 1 cut(s) 879
BcgI CGANNNNNNTGC 2 cut(s) 918, 952
BcoDI GTCTC 4 cut(s) 31, 304, 902, 1119
BfaI CTAG 3 cut(s) 296, 360, 597
BisI GCNGC 1 cut(s) 893
BlpI GCTNAGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 894
BmcAI AGTACT 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 2 cut(s) 19, 626
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 557
Bpu10I CCTNAGC 1 cut(s) 873
Bpu1102I GCTNAGC 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 92, 843, 936
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 118, 844
Bse1I ACTGG 3 cut(s) 142, 421, 425
BseDI CCNNGG 3 cut(s) 92, 843, 936
BseGI GGATG 2 cut(s) 205, 634
BseLI CCNNNNNNNGG 3 cut(s) 117, 118, 844
BseMII CTCAG 5 cut(s) 315, 777, 864, 969, 1132
BseNI ACTGG 3 cut(s) 142, 421, 425
BseRI GAGGAG 3 cut(s) 195, 767, 964
BseSI GKGCMC 1 cut(s) 960
BseXI GCAGC 1 cut(s) 879
BshFI GGCC 2 cut(s) 20, 628
BsiHKAI GWGCWC 1 cut(s) 960
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 3 cut(s) 117, 118, 844
BsmAI GTCTC 4 cut(s) 31, 304, 902, 1119
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 2 cut(s) 20, 628
Bsp1286I GDGCHC 1 cut(s) 960
Bsp143I GATC 3 cut(s) 432, 601, 834
Bsp1720I GCTNAGC 1 cut(s) 786
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 2 cut(s) 355, 430
BspANI GGCC 2 cut(s) 20, 628
BspCNI CTCAG 5 cut(s) 314, 778, 865, 970, 1131
BspHI TCATGA 1 cut(s) 880
BspPI GGATC 3 cut(s) 440, 596, 829
BsrI ACTGG 3 cut(s) 142, 421, 425
BssECI CCNNGG 3 cut(s) 92, 843, 936
BssMI GATC 3 cut(s) 432, 601, 834
BssT1I CCWWGG 2 cut(s) 92, 843
Bst4CI ACNGT 3 cut(s) 146, 156, 448
Bst6I CTCTTC 4 cut(s) 166, 478, 636, 804
BstC8I GCNNGC 2 cut(s) 77, 1124
BstDEI CTNAG 7 cut(s) 165, 301, 376, 786, 873, 978, 1118
BstDSI CCRYGG 1 cut(s) 843
BstF5I GGATG 2 cut(s) 205, 634
BstKTI GATC 3 cut(s) 435, 604, 837
BstMAI GTCTC 4 cut(s) 31, 304, 902, 1119
BstMBI GATC 3 cut(s) 432, 601, 834
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 879
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 601, 834
BstYI RGATCY 2 cut(s) 601, 834
BsuRI GGCC 2 cut(s) 20, 628
BtgI CCRYGG 1 cut(s) 843
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 2 cut(s) 205, 634
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 5 cut(s) 414, 432, 444, 539, 821
Cac8I GCNNGC 2 cut(s) 77, 1124
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 2 cut(s) 19, 626
Csp6I GTAC 2 cut(s) 210, 373
CviAII CATG 5 cut(s) 33, 571, 844, 881, 1027
CviQI GTAC 2 cut(s) 210, 373
DdeI CTNAG 7 cut(s) 165, 301, 376, 786, 873, 978, 1118
DpnI GATC 3 cut(s) 434, 603, 836
DpnII GATC 3 cut(s) 432, 601, 834
Eam1104I CTCTTC 4 cut(s) 166, 478, 636, 804
EarI CTCTTC 4 cut(s) 166, 478, 636, 804
Eco130I CCWWGG 2 cut(s) 92, 843
Eco57I CTGAAG 1 cut(s) 870
EcoT14I CCWWGG 2 cut(s) 92, 843
ErhI CCWWGG 2 cut(s) 92, 843
FaeI CATG 5 cut(s) 36, 574, 847, 884, 1030
FaqI GGGAC 1 cut(s) 753
FatI CATG 5 cut(s) 32, 570, 843, 880, 1026
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 1 cut(s) 893
FokI GGATG 2 cut(s) 212, 621
Fsp4HI GCNGC 1 cut(s) 893
FspBI CTAG 3 cut(s) 296, 360, 597
GluI GCNGC 1 cut(s) 893
HaeIII GGCC 2 cut(s) 20, 628
Hin1II CATG 5 cut(s) 36, 574, 847, 884, 1030
HincII GTYRAC 3 cut(s) 99, 149, 469
HindII GTYRAC 3 cut(s) 99, 149, 469
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 4 cut(s) 363, 973, 994, 1065
HpaI GTTAAC 1 cut(s) 469
HphI GGTGA 3 cut(s) 721, 1015, 1085
Hpy166II GTNNAC 5 cut(s) 99, 149, 393, 469, 958
Hpy188I TCNGA 4 cut(s) 437, 940, 999, 1054
Hpy188III TCNNGA 5 cut(s) 360, 367, 638, 881, 977
Hpy8I GTNNAC 5 cut(s) 99, 149, 393, 469, 958
HpyAV CCTTC 3 cut(s) 269, 647, 679
HpyCH4III ACNGT 3 cut(s) 146, 156, 448
HpyCH4IV ACGT 2 cut(s) 58, 742
HpyCH4V TGCA 4 cut(s) 188, 320, 929, 958
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 7 cut(s) 165, 301, 376, 786, 873, 978, 1118
HpySE526I ACGT 2 cut(s) 58, 742
Hsp92II CATG 5 cut(s) 36, 574, 847, 884, 1030
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 3 cut(s) 432, 601, 834
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 1 cut(s) 879
LweI GCATC 1 cut(s) 190
MaeI CTAG 3 cut(s) 296, 360, 597
MaeII ACGT 2 cut(s) 58, 742
MaeIII GTNAC 3 cut(s) 54, 442, 1101
MalI GATC 3 cut(s) 434, 603, 836
MboI GATC 3 cut(s) 432, 601, 834
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 2 cut(s) 601, 834
MhlI GDGCHC 1 cut(s) 960
MluCI AATT 9 cut(s) 10, 104, 350, 535, 582, 606, 660, 713, 1093
MlyI GAGTC 1 cut(s) 967
MmeI TCCRAC 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 855, 993
MseI TTAA 2 cut(s) 107, 468
MslI CAYNNNNRTG 1 cut(s) 885
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 432, 601, 834
NlaIII CATG 5 cut(s) 36, 574, 847, 884, 1030
NmuCI GTSAC 2 cut(s) 54, 442
NspI RCATGY 1 cut(s) 36
PagI TCATGA 1 cut(s) 880
PctI GAATGC 1 cut(s) 647
PdmI GAANNNNTTC 2 cut(s) 855, 993
PfeI GAWTC 3 cut(s) 363, 994, 1065
PflFI GACNNNGTC 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 844
PkrI GCNGC 1 cut(s) 894
PleI GAGTC 1 cut(s) 967
PpsI GAGTC 1 cut(s) 967
PspPI GGNCC 2 cut(s) 19, 626
PsuI RGATCY 2 cut(s) 601, 834
PsyI GACNNNGTC 1 cut(s) 144
RsaI GTAC 2 cut(s) 211, 374
RsaNI GTAC 2 cut(s) 210, 373
RseI CAYNNNNRTG 1 cut(s) 885
SaqAI TTAA 2 cut(s) 107, 468
SatI GCNGC 1 cut(s) 893
Sau3AI GATC 3 cut(s) 432, 601, 834
Sau96I GGNCC 2 cut(s) 19, 626
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 1 cut(s) 967
SduI GDGCHC 1 cut(s) 960
SfaNI GCATC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 9 cut(s) 10, 104, 350, 535, 582, 606, 660, 713, 1093
SsiI CCGC 2 cut(s) 355, 430
SspI AATATT 2 cut(s) 181, 616
SspMI CTAG 3 cut(s) 296, 360, 597
StyI CCWWGG 2 cut(s) 92, 843
TaaI ACNGT 3 cut(s) 146, 156, 448
TaiI ACGT 2 cut(s) 61, 745
TaqI TCGA 2 cut(s) 489, 532
TasI AATT 9 cut(s) 10, 104, 350, 535, 582, 606, 660, 713, 1093
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 3 cut(s) 363, 994, 1065
Tru1I TTAA 2 cut(s) 107, 468
Tru9I TTAA 2 cut(s) 107, 468
TscAI CASTG 5 cut(s) 421, 432, 451, 546, 828
TseFI GTSAC 2 cut(s) 54, 442
TseI GCWGC 1 cut(s) 892
Tsp45I GTSAC 2 cut(s) 54, 442
TspDTI ATGAA 5 cut(s) 623, 698, 726, 869, 1043
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 5 cut(s) 421, 432, 451, 546, 828
Tth111I GACNNNGTC 1 cut(s) 144
Van91I CCANNNNNTGG 1 cut(s) 844
VneI GTGCAC 1 cut(s) 956
XapI RAATTY 3 cut(s) 10, 535, 1093
XbaI TCTAGA 1 cut(s) 359
XceI RCATGY 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 855, 993
XspI CTAG 3 cut(s) 296, 360, 597
ZrmI AGTACT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.