FvH4_1g24160

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012178
Physical Location & Seq
Reverse (-)
16027812 .. 16029053
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24160.t1

Sequence Viewer

Length: 555 bp
ATGGGATCTAGTTCCACTACTACTTCTGCTACATTCTCCTTGGTTATGCTGTTTCTACTTCTACAACATGATCATTTTGCTACTGCAGACACTAGCTTGATCCAGAAAACCTGCAAAGCTACCAAGTACTATGCCCTTTGCATGTCATCCCTCAAGTCCAATCCCACCAGCCTAACCGCAGACGGCAAGGGCCTGGCTGCTATTATTGTCGGAATCGCGACCGCGAATGCCACCGCAACCTCTTCTTACTTGTCCTCCCAAGTGCTCAGCTCGGCCAACGACGCCAACGCCAAGAAGGTGCTAAAGGAGTGTGCTGACAAGTATGGCTACGCGGGCGAGGCCCTGCAGGGTTCGCTGCAGGACCTGGCCGCGGAGTCTTACGACTACGCTTACATGCACATAACTGCAGCTGCCGACTACCCTAATGCATGCCACAATGCGTTTAGGAGGTTTCCGGCGTTGGTTTATCCACCGGAGCTTGCAAGAAGAGAGGAGGCTTTGAAGCGGATCTGTAGTGTGGTTTTGGGAATTATTGATAGTTTTGGCTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009628 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0080167 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

185

Amino Acids

19.7

Weight (kDa)

6.4

Isoelectric Point (pI)

57.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 176 3.9e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64620
fragaria_vesca FvH4_1g24160
malus_domestica MD01G1038300.v1.1 MD15G1314600.v1.1
prunus_persica Prupe.6G197400_v2.0.a1
pyrus_communis pycom01g06630 pycom15g27810
rosa_chinensis RchiOBHm_Chr2g0120401
rosa_laevigata RLG00000018555
rosa_multiflora Rmu_co8012200.1_g000001 Rmu_sc0007380.1_g000001 Rmu_sc0007503.1_g000005
rosa_roxburghii Rroxscaffold_2G00123440
rosa_rugosa Rorug02G0229400
rosa_samantha Rh2AG285300 Rh2BG296400 Rh2CG274100 Rh2DG310300
rosa_wichuraiana Rw2G022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 119
AccII CGCG 4 cut(s) 218, 224, 332, 371
AciI CCGC 7 cut(s) 177, 222, 234, 332, 369, 371, 505
AclWI GGATC 3 cut(s) 13, 94, 515
AcoI YGGCCR 2 cut(s) 273, 366
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 370
AgsI TTSAA 1 cut(s) 502
AjnI CCWGG 2 cut(s) 192, 363
AluBI AGCT 5 cut(s) 96, 119, 270, 410, 478
AluI AGCT 5 cut(s) 96, 119, 270, 410, 478
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 3 cut(s) 13, 94, 515
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 4 cut(s) 190, 273, 339, 366
ApeKI GCWGC 4 cut(s) 197, 355, 407, 410
AspS9I GGNCC 3 cut(s) 190, 340, 361
AvaII GGWCC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 184, 342, 397, 419
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 2 cut(s) 194, 365
BclI TGATCA 1 cut(s) 70
BfaI CTAG 2 cut(s) 9, 93
BfmI CTRYAG 5 cut(s) 84, 344, 356, 405, 511
BfuAI ACCTGC 1 cut(s) 119
BisI GCNGC 5 cut(s) 198, 356, 369, 408, 411
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 5 cut(s) 199, 357, 370, 409, 412
BmcAI AGTACT 1 cut(s) 128
Bme1390I CCNGG 2 cut(s) 194, 365
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 3 cut(s) 190, 340, 361
BmrFI CCNGG 2 cut(s) 194, 365
Bpu1102I GCTNAGC 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 39, 369
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 370
BseBI CCWGG 2 cut(s) 194, 365
BseDI CCNNGG 2 cut(s) 39, 369
BseGI GGATG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMII CTCAG 1 cut(s) 280
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 4 cut(s) 184, 342, 397, 419
BseYI CCCAGC 1 cut(s) 546
Bsh1236I CGCG 4 cut(s) 218, 224, 332, 371
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 192, 275, 341, 368
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 2 cut(s) 455, 473
BslI CCNNNNNNNGG 1 cut(s) 370
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 4 cut(s) 192, 275, 341, 368
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 5, 70, 99, 507
Bsp1720I GCTNAGC 1 cut(s) 266
Bsp68I TCGCGA 1 cut(s) 218
BspACI CCGC 7 cut(s) 177, 222, 234, 332, 369, 371, 505
BspANI GGCC 4 cut(s) 192, 275, 341, 368
BspCNI CTCAG 1 cut(s) 279
BspFNI CGCG 4 cut(s) 218, 224, 332, 371
BspMAI CTGCAG 4 cut(s) 88, 348, 360, 409
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 3 cut(s) 13, 94, 515
BssECI CCNNGG 2 cut(s) 39, 369
BssMI GATC 4 cut(s) 5, 70, 99, 507
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 194, 365
Bst6I CTCTTC 2 cut(s) 247, 481
BstACI GRCGYC 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 334, 430, 480
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 146
BstFNI CGCG 4 cut(s) 218, 224, 332, 371
BstKTI GATC 4 cut(s) 8, 73, 102, 510
BstMBI GATC 4 cut(s) 5, 70, 99, 507
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 281, 333, 338, 352
BstNI CCWGG 2 cut(s) 194, 365
BstNSI RCATGY 3 cut(s) 145, 397, 432
BstSCI CCNGG 2 cut(s) 192, 363
BstSFI CTRYAG 5 cut(s) 84, 344, 356, 405, 511
BstUI CGCG 4 cut(s) 218, 224, 332, 371
BstV1I GCAGC 4 cut(s) 184, 342, 397, 419
BstX2I RGATCY 2 cut(s) 5, 507
BstYI RGATCY 2 cut(s) 5, 507
BsuRI GGCC 4 cut(s) 192, 275, 341, 368
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 146
BtuMI TCGCGA 1 cut(s) 218
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 334, 430, 480
CaiI CAGNNNCTG 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 190, 340, 361
Cfr42I CCGCGG 1 cut(s) 372
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 127
CviAII CATG 4 cut(s) 68, 142, 394, 429
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 1 cut(s) 266
DpnI GATC 4 cut(s) 7, 72, 101, 509
DpnII GATC 4 cut(s) 5, 70, 99, 507
EaeI YGGCCR 2 cut(s) 273, 366
Eam1104I CTCTTC 2 cut(s) 247, 481
EarI CTCTTC 2 cut(s) 247, 481
Eco130I CCWWGG 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 361
EcoO109I RGGNCCY 3 cut(s) 190, 340, 361
EcoRII CCWGG 2 cut(s) 192, 363
EcoT14I CCWWGG 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 4 cut(s) 71, 145, 397, 432
FaiI YATR 8 cut(s) 47, 69, 132, 143, 324, 395, 401, 430
FatI CATG 4 cut(s) 67, 141, 393, 428
FauI CCCGC 1 cut(s) 325
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 5 cut(s) 198, 356, 369, 408, 411
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 5 cut(s) 198, 356, 369, 408, 411
FspBI CTAG 2 cut(s) 9, 93
GluI GCNGC 5 cut(s) 198, 356, 369, 408, 411
GsaI CCCAGC 1 cut(s) 550
HaeIII GGCC 4 cut(s) 192, 275, 341, 368
HapII CCGG 2 cut(s) 455, 473
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 71, 145, 397, 432
HinfI GANTC 2 cut(s) 213, 374
HpaII CCGG 2 cut(s) 455, 473
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 2 cut(s) 103, 217
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 1 cut(s) 289
HpyCH4V TGCA 9 cut(s) 86, 114, 141, 346, 358, 397, 407, 428, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 333, 338, 352
HpyF3I CTNAG 1 cut(s) 266
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 71, 145, 397, 432
Ksp22I TGATCA 1 cut(s) 70
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 4 cut(s) 5, 70, 99, 507
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 4 cut(s) 184, 342, 397, 419
MaeI CTAG 2 cut(s) 9, 93
MalI GATC 4 cut(s) 7, 72, 101, 509
MboI GATC 4 cut(s) 5, 70, 99, 507
MboII GAAGA 2 cut(s) 234, 498
MflI RGATCY 2 cut(s) 5, 507
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 528
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 7 cut(s) 161, 250, 265, 331, 441, 484, 487
Mph1103I ATGCAT 1 cut(s) 430
MspA1I CMGCKG 2 cut(s) 371, 410
MspI CCGG 2 cut(s) 455, 473
MspR9I CCNGG 2 cut(s) 194, 365
Mva1269I GAATGC 1 cut(s) 232
MvaI CCWGG 2 cut(s) 194, 365
MvnI CGCG 4 cut(s) 218, 224, 332, 371
MwoI GCNNNNNNNGC 4 cut(s) 281, 333, 338, 352
NdeII GATC 4 cut(s) 5, 70, 99, 507
NlaIII CATG 4 cut(s) 71, 145, 397, 432
NmeAIII GCCGAG 1 cut(s) 251
NruI TCGCGA 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 3 cut(s) 145, 397, 432
PaeI GCATGC 1 cut(s) 432
PctI GAATGC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 5 cut(s) 199, 357, 370, 409, 412
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 2 cut(s) 192, 363
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 2 cut(s) 192, 363
PspPI GGNCC 3 cut(s) 190, 340, 361
PspPPI RGGWCCY 1 cut(s) 361
PstI CTGCAG 4 cut(s) 88, 348, 360, 409
PstNI CAGNNNCTG 1 cut(s) 364
PsuI RGATCY 2 cut(s) 5, 507
PvuII CAGCTG 1 cut(s) 410
RruI TCGCGA 1 cut(s) 218
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacII CCGCGG 1 cut(s) 372
SatI GCNGC 5 cut(s) 198, 356, 369, 408, 411
Sau3AI GATC 4 cut(s) 5, 70, 99, 507
Sau96I GGNCC 3 cut(s) 190, 340, 361
SbfI CCTGCAGG 1 cut(s) 348
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 194, 365
SdaI CCTGCAGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 267
SfcI CTRYAG 5 cut(s) 84, 344, 356, 405, 511
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SinI GGWCC 1 cut(s) 361
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SphI GCATGC 1 cut(s) 432
Sse8387I CCTGCAGG 1 cut(s) 348
Sse9I AATT 1 cut(s) 528
SsiI CCGC 7 cut(s) 177, 222, 234, 332, 369, 371, 505
SspMI CTAG 2 cut(s) 9, 93
StyD4I CCNGG 2 cut(s) 192, 363
StyI CCWWGG 1 cut(s) 39
TasI AATT 1 cut(s) 528
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 213
TseI GCWGC 4 cut(s) 197, 355, 407, 410
VpaK11BI GGWCC 1 cut(s) 361
XceI RCATGY 3 cut(s) 145, 397, 432
XspI CTAG 2 cut(s) 9, 93
ZrmI AGTACT 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.