MD01G1038300.v1.1

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012178
Physical Location & Seq
Forward (+)
13148880 .. 13149428
549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1038300.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGTTATTCTACTATTTTCTTGCTCTTTCTTCTCACAACTACATTTCCTCACCAAAACCCTAATTTTCTCGTCCATGCTGACTCAACCCTGATCCAGAACACATGCAAAAACACCAAGTACTATGGCCTCTGCGTCTCATCCCTCAAATCTGTTCCCACAAGCAAAAATGCAGACACAAAGGGGTTGGCAGTTATTATGGTTGGGATTGGAATGGCAAATGCAACCGCCACCTCATCCTACTTGTCCTCTCAACTCATTAGCTCAAGCACAACCGACACAAACTTGAAGAAGGTCCTCAAGGAGTGTGCCGACAAGTACGGCTACGCCGGCGATGCCCTTCAAGCTTCAGTGCAGGATTTGGGCTCAGAGTCTTACGACTATGCTTACATGCACATCACTGCAGCCGCAGACTACCCTAATGCCTGCCACAATGCTTTCAAAAGGTACCCAGGTTTGGCTTATCCTCCAGAGCTTGCAAGGAGAGAGGAGGGTTTGAAGCATATTTGTGACGTGGCTCTGGGAATTATTGATAATTTTGGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009628 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0080167 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

183

Amino Acids

19.69

Weight (kDa)

6.49

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 175 2e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64620
fragaria_vesca FvH4_1g24160
malus_domestica MD01G1038300.v1.1 MD15G1314600.v1.1
prunus_persica Prupe.6G197400_v2.0.a1
pyrus_communis pycom01g06630 pycom15g27810
rosa_chinensis RchiOBHm_Chr2g0120401
rosa_laevigata RLG00000018555
rosa_multiflora Rmu_co8012200.1_g000001 Rmu_sc0007380.1_g000001 Rmu_sc0007503.1_g000005
rosa_roxburghii Rroxscaffold_2G00123440
rosa_rugosa Rorug02G0229400
rosa_samantha Rh2AG285300 Rh2BG296400 Rh2CG274100 Rh2DG310300
rosa_wichuraiana Rw2G022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 447
AciI CCGC 2 cut(s) 228, 408
AclWI GGATC 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 3 cut(s) 122, 320, 449
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 4 cut(s) 289, 344, 442, 499
AjiI CACGTC 1 cut(s) 514
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 3 cut(s) 264, 347, 475
AluI AGCT 3 cut(s) 264, 347, 475
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 1 cut(s) 88
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 295
BaeI ACNNNNGTAYC 2 cut(s) 439, 472
BanI GGYRCC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 416
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 142
BfmI CTRYAG 1 cut(s) 402
BisI GCNGC 2 cut(s) 405, 408
BlsI GCNGC 2 cut(s) 406, 409
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 1 cut(s) 295
BmgBI CACGTC 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 250, 284
BsaJI CCNNGG 1 cut(s) 451
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse118I RCCGGY 1 cut(s) 329
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 140, 236
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 381
BseRI GAGGAG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 447
BsiSI CCGG 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 142
BsmBI CGTCTC 1 cut(s) 142
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 1 cut(s) 93
BspACI CCGC 2 cut(s) 228, 408
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 449
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 447
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 453
BstC8I GCNNGC 3 cut(s) 331, 427, 477
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 140, 236
BstKTI GATC 1 cut(s) 96
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 1 cut(s) 93
BstMWI GCNNNNNNNGC 3 cut(s) 330, 335, 344
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 2 cut(s) 108, 394
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 416
BsuRI GGCC 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 2 cut(s) 140, 236
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 357, 399
Cac8I GCNNGC 3 cut(s) 331, 427, 477
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 295
CseI GACGC 1 cut(s) 124
Csp6I GTAC 3 cut(s) 121, 319, 448
CviAII CATG 3 cut(s) 77, 105, 391
CviJI RGCY 9 cut(s) 129, 264, 324, 347, 366, 407, 461, 475, 518
CviKI_1 RGCY 9 cut(s) 129, 264, 324, 347, 366, 407, 461, 475, 518
CviQI GTAC 3 cut(s) 121, 319, 448
DdeI CTNAG 1 cut(s) 367
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 3 cut(s) 80, 108, 394
FaiI YATR 7 cut(s) 78, 106, 126, 200, 384, 392, 504
FatI CATG 3 cut(s) 76, 104, 390
Fnu4HI GCNGC 2 cut(s) 405, 408
FokI GGATG 2 cut(s) 127, 223
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 2 cut(s) 405, 408
GluI GCNGC 2 cut(s) 405, 408
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 330
HgaI GACGC 1 cut(s) 124
Hin1II CATG 3 cut(s) 80, 108, 394
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 2 cut(s) 83, 371
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 44
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 2 cut(s) 97, 470
HpyAV CCTTC 2 cut(s) 286, 350
HpyCH4IV ACGT 1 cut(s) 513
HpyCH4V TGCA 7 cut(s) 108, 173, 224, 355, 394, 404, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 330, 335, 344
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 1 cut(s) 513
Hsp92II CATG 3 cut(s) 80, 108, 394
KpnI GGTACC 1 cut(s) 451
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 1 cut(s) 93
LpnPI CCDG 9 cut(s) 104, 110, 341, 343, 438, 439, 465, 483, 506
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 1 cut(s) 325
MaeII ACGT 1 cut(s) 513
MaeIII GTNAC 1 cut(s) 509
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 2 cut(s) 23, 301
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 3 cut(s) 64, 525, 535
MlyI GAGTC 2 cut(s) 77, 380
MnlI CCTC 9 cut(s) 60, 140, 155, 244, 259, 308, 477, 481, 484
MreI CGCCGGCG 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 329
MslI CAYNNNNRTG 1 cut(s) 507
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 3 cut(s) 330, 335, 344
NaeI GCCGGC 1 cut(s) 331
NdeII GATC 1 cut(s) 93
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 3 cut(s) 80, 108, 394
NlaIV GGNNCC 1 cut(s) 449
NmuCI GTSAC 1 cut(s) 509
NspI RCATGY 2 cut(s) 108, 394
PdiI GCCGGC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 406, 409
PleI GAGTC 2 cut(s) 77, 379
PpsI GAGTC 2 cut(s) 77, 379
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 449
PspPI GGNCC 1 cut(s) 295
PspPPI RGGWCCY 1 cut(s) 295
PstI CTGCAG 1 cut(s) 406
RsaI GTAC 3 cut(s) 122, 320, 449
RsaNI GTAC 3 cut(s) 121, 319, 448
RseI CAYNNNNRTG 1 cut(s) 507
SatI GCNGC 2 cut(s) 405, 408
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 1 cut(s) 295
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 2 cut(s) 77, 380
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 368
SetI ASST 8 cut(s) 236, 266, 297, 349, 449, 457, 477, 516
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 402
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 265, 299
SmoI CTYRAG 2 cut(s) 265, 299
Sse9I AATT 3 cut(s) 64, 525, 535
SsiI CCGC 2 cut(s) 228, 408
StyD4I CCNGG 1 cut(s) 451
TaiI ACGT 1 cut(s) 516
TasI AATT 3 cut(s) 64, 525, 535
TatI WGTACW 1 cut(s) 120
TauI GCSGC 1 cut(s) 410
TscAI CASTG 2 cut(s) 357, 406
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 509
TspRI CASTG 2 cut(s) 357, 406
VpaK11BI GGWCC 1 cut(s) 295
XceI RCATGY 2 cut(s) 108, 394
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.