RLG00000018555

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012178
Physical Location & Seq
Reverse (-)
31783966 .. 31784517
552 bp
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UTR
Exon/CDS
Intron
RLM00000018555

Sequence Viewer

Length: 552 bp
ATGGGATCTAGTGCTACTTCTGCTACATTCTCCTTGGTTATTCTTCAACTACTACTTCTACAACATGATCATTTTGCGAGTGCAGACTCTAGCTTGATCCAGAAAACCTGCAAAGCCACCAAGTACTATGCCCTTTGCATGTCCTCCCTCAAGTCCGATCCCACCAGCCTAACCGCAGACGGCAAGGGCTTGGCAGCTATTATTGTCAGAATTGCGACCACCAATGCCACCGCAACCTCTTCTTACTTGTCCTCCCAAGTGCTCAGCTCTGCAAACGACGCCAACGCCAAGAAGGTGCTGAAGGAGTGTGCTGACAAGTATGGCTACGCCGGTGAGGCCCTGCAGGGTTCGCTGCAGGACCTGGCGGCGGAGTCTTATGACTACGCTTACATGCACATCACTGCAGCTGCCGACTACCCTAATGCTTGCCACAATGCGTTTAGAAGGTATCCGGCGTTGGCTTATCCACCGGAGCTTGCTAGAAGAGAGGATGCTTTGAAGCGGATTTGTGATGTGGTTTTGGGAATTCTTGATAGTCTTGGTTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009628 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0080167 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

184

Amino Acids

19.63

Weight (kDa)

6.05

Isoelectric Point (pI)

63.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 175 2.2e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64620
fragaria_vesca FvH4_1g24160
malus_domestica MD01G1038300.v1.1 MD15G1314600.v1.1
prunus_persica Prupe.6G197400_v2.0.a1
pyrus_communis pycom01g06630 pycom15g27810
rosa_chinensis RchiOBHm_Chr2g0120401
rosa_laevigata RLG00000018555
rosa_multiflora Rmu_co8012200.1_g000001 Rmu_sc0007380.1_g000001 Rmu_sc0007503.1_g000005
rosa_roxburghii Rroxscaffold_2G00123440
rosa_rugosa Rorug02G0229400
rosa_samantha Rh2AG285300 Rh2BG296400 Rh2CG274100 Rh2DG310300
rosa_wichuraiana Rw2G022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 116
AciI CCGC 5 cut(s) 174, 231, 365, 368, 502
AclWI GGATC 3 cut(s) 13, 91, 152
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 2 cut(s) 47, 499
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 5 cut(s) 93, 197, 267, 407, 475
AluI AGCT 5 cut(s) 93, 197, 267, 407, 475
Alw21I GWGCWC 1 cut(s) 264
AlwI GGATC 3 cut(s) 13, 91, 152
AlwNI CAGNNNCTG 1 cut(s) 361
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 4 cut(s) 194, 352, 404, 407
ApoI RAATTY 1 cut(s) 525
AspS9I GGNCC 2 cut(s) 337, 358
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 4 cut(s) 206, 339, 394, 416
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 1 cut(s) 362
BciVI GTATCC 1 cut(s) 459
BclI TGATCA 1 cut(s) 67
BfaI CTAG 3 cut(s) 9, 90, 480
BfmI CTRYAG 3 cut(s) 341, 353, 402
BfuAI ACCTGC 1 cut(s) 116
BfuI GTATCC 1 cut(s) 459
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 5 cut(s) 195, 353, 366, 405, 408
BlpI GCTNAGC 1 cut(s) 263
BlsI GCNGC 5 cut(s) 196, 354, 367, 406, 409
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 337, 358
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 481
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 1 cut(s) 329
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMII CTCAG 1 cut(s) 277
BseXI GCAGC 4 cut(s) 206, 339, 394, 416
BsgI GTGCAG 1 cut(s) 102
BshFI GGCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 330, 452, 470
BslI CCNNNNNNNGG 1 cut(s) 367
BsnI GGCC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 4 cut(s) 5, 67, 96, 157
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 5 cut(s) 174, 231, 365, 368, 502
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 1 cut(s) 276
BspMAI CTGCAG 3 cut(s) 345, 357, 406
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 3 cut(s) 13, 91, 152
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 4 cut(s) 5, 67, 96, 157
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 362
Bst6I CTCTTC 2 cut(s) 244, 478
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 427, 477
BstDEI CTNAG 1 cut(s) 263
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 4 cut(s) 8, 70, 99, 160
BstMBI GATC 4 cut(s) 5, 67, 96, 157
BstMWI GCNNNNNNNGC 4 cut(s) 20, 278, 335, 349
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 2 cut(s) 142, 394
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 3 cut(s) 341, 353, 402
BstV1I GCAGC 4 cut(s) 206, 339, 394, 416
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuI GTATCC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 338
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 2 cut(s) 427, 477
CaiI CAGNNNCTG 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 337, 358
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 65, 139, 391
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 263
DpnI GATC 4 cut(s) 7, 69, 98, 159
DpnII GATC 4 cut(s) 5, 67, 96, 157
Eam1104I CTCTTC 2 cut(s) 244, 478
EarI CTCTTC 2 cut(s) 244, 478
EciI GGCGGA 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 320
EcoO109I RGGNCCY 2 cut(s) 337, 358
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 68, 142, 394
FaiI YATR 6 cut(s) 66, 129, 140, 321, 378, 392
FatI CATG 3 cut(s) 64, 138, 390
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 5 cut(s) 195, 353, 366, 405, 408
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 5 cut(s) 195, 353, 366, 405, 408
FspBI CTAG 3 cut(s) 9, 90, 480
GluI GCNGC 5 cut(s) 195, 353, 366, 405, 408
HaeIII GGCC 1 cut(s) 338
HapII CCGG 3 cut(s) 330, 452, 470
HgaI GACGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 3 cut(s) 68, 142, 394
HinfI GANTC 2 cut(s) 86, 371
HpaII CCGG 3 cut(s) 330, 452, 470
HphI GGTGA 1 cut(s) 344
Hpy188I TCNGA 2 cut(s) 157, 209
Hpy188III TCNNGA 2 cut(s) 100, 530
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 3 cut(s) 286, 295, 438
HpyCH4V TGCA 8 cut(s) 83, 111, 138, 272, 343, 355, 394, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 278, 335, 349
HpyF3I CTNAG 1 cut(s) 263
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 3 cut(s) 68, 142, 394
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 4 cut(s) 5, 67, 96, 157
LmnI GCTCC 1 cut(s) 472
Lsp1109I GCAGC 4 cut(s) 206, 339, 394, 416
LweI GCATC 1 cut(s) 481
MaeI CTAG 3 cut(s) 9, 90, 480
MalI GATC 4 cut(s) 7, 69, 98, 159
MboI GATC 4 cut(s) 5, 67, 96, 157
MboII GAAGA 3 cut(s) 35, 231, 495
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 2 cut(s) 210, 525
MlyI GAGTC 2 cut(s) 80, 380
MnlI CCTC 6 cut(s) 154, 158, 247, 262, 328, 481
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 3 cut(s) 330, 452, 470
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 4 cut(s) 20, 278, 335, 349
NdeII GATC 4 cut(s) 5, 67, 96, 157
NlaIII CATG 3 cut(s) 68, 142, 394
NspI RCATGY 2 cut(s) 142, 394
PkrI GCNGC 5 cut(s) 196, 354, 367, 406, 409
PleI GAGTC 2 cut(s) 80, 379
PpsI GAGTC 2 cut(s) 80, 379
PpuMI RGGWCCY 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspPI GGNCC 2 cut(s) 337, 358
PspPPI RGGWCCY 1 cut(s) 358
PstI CTGCAG 3 cut(s) 345, 357, 406
PstNI CAGNNNCTG 1 cut(s) 361
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 5 cut(s) 195, 353, 366, 405, 408
Sau3AI GATC 4 cut(s) 5, 67, 96, 157
Sau96I GGNCC 2 cut(s) 337, 358
SbfI CCTGCAGG 1 cut(s) 345
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 2 cut(s) 80, 380
ScrFI CCNGG 1 cut(s) 362
SdaI CCTGCAGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 3 cut(s) 341, 353, 402
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse8387I CCTGCAGG 1 cut(s) 345
Sse9I AATT 2 cut(s) 210, 525
SsiI CCGC 5 cut(s) 174, 231, 365, 368, 502
SspMI CTAG 3 cut(s) 9, 90, 480
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 33
TasI AATT 2 cut(s) 210, 525
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 368
TscAI CASTG 1 cut(s) 406
TseI GCWGC 4 cut(s) 194, 352, 404, 407
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 525
XceI RCATGY 2 cut(s) 142, 394
XspI CTAG 3 cut(s) 9, 90, 480
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.