pycom01g06630

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012178
Physical Location & Seq
Forward (+)
7027959 .. 7028627
669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06630.1

Sequence Viewer

Length: 669 bp
ATGCATCTCCTACATTGCTTTTCAACCATAGAAAAATACCTAATATCTACTTTTCCTTCGCTCTCTTCCTCCACACCAAAATCTAGAGCCAAAAAAAGAAAGAAAAAAGTATACTCTCAGATGGGTTCGTCTACTATTTTCTTGCTCTTTCTTCTCACAATTGCATTTCCTCACCAAAACCCTAATTTTCTCGTCCGTGCTGACTCAACCCTGATCCAGAACACATGCAAAAACACCAAGTACTATGGCCTCTGCGTCTCATCCCTCAAATCTGTTCCCACAAGCAAAAATGCAGACACAAAGGGGTTGGCAGTTATTATGGTTGGGATTGGAATGGCAAATGCAACCGCCACCTCATCCTACTTGTCCTCTCAACTCATTAGCTCAAGTACAACCGAAACAAATTTGAAGAAGGTCCTCAAGGAGTGTGCCGACAAGTACGGCTACGCCGGCGATGCCCTTCAAGCTTCAGTGCAGGATTTGGGCTCAGAGTCTTATGACTATGCTTACATGCACATCACTGCAGCCGCAGACTACCCGAATGCCTGCCACAATGCTTTCAAAAGGTACCCAGGTTTGGCTTATCCTCCAGAGCTTGCAAGAAGAGAGGAGGGTTTGAAGCATATTTGTGACGTGGCTCTGGGAATTATTGATAATTTTAGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009628 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0080167 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

223

Amino Acids

24.29

Weight (kDa)

9.12

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 71 - 215 4.2e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64620
fragaria_vesca FvH4_1g24160
malus_domestica MD01G1038300.v1.1 MD15G1314600.v1.1
prunus_persica Prupe.6G197400_v2.0.a1
pyrus_communis pycom01g06630 pycom15g27810
rosa_chinensis RchiOBHm_Chr2g0120401
rosa_laevigata RLG00000018555
rosa_multiflora Rmu_co8012200.1_g000001 Rmu_sc0007380.1_g000001 Rmu_sc0007503.1_g000005
rosa_roxburghii Rroxscaffold_2G00123440
rosa_rugosa Rorug02G0229400
rosa_samantha Rh2AG285300 Rh2BG296400 Rh2CG274100 Rh2DG310300
rosa_wichuraiana Rw2G022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 567
AccI GTMKAC 2 cut(s) 111, 131
AciI CCGC 2 cut(s) 348, 528
AclWI GGATC 1 cut(s) 208
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 4 cut(s) 242, 391, 440, 569
AfiI CCNNNNNNNGG 1 cut(s) 577
AgsI TTSAA 5 cut(s) 24, 409, 464, 562, 619
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 1 cut(s) 571
AluBI AGCT 3 cut(s) 384, 467, 595
AluI AGCT 3 cut(s) 384, 467, 595
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 1 cut(s) 208
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 1 cut(s) 403
Asp718I GGTACC 1 cut(s) 567
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 559, 592
BanI GGYRCC 1 cut(s) 567
BanII GRGCYC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 536
BccI CCATC 1 cut(s) 115
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 2 cut(s) 525, 528
BlsI GCNGC 2 cut(s) 526, 529
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 573
Bme18I GGWCC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 569
BmrFI CCNGG 1 cut(s) 573
BmsI GCATC 2 cut(s) 13, 445
BpmI CTGGAG 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 370, 404
BsaJI CCNNGG 1 cut(s) 571
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse118I RCCGGY 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 2 cut(s) 260, 356
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 131, 501
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 1 cut(s) 536
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 262
BsmBI CGTCTC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 2 cut(s) 348, 528
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 2 cut(s) 130, 500
BspLI GGNNCC 1 cut(s) 569
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 1 cut(s) 213
BssNAI GTATAC 1 cut(s) 112
Bst1107I GTATAC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 573
Bst6I CTCTTC 2 cut(s) 70, 598
BstC8I GCNNGC 3 cut(s) 451, 547, 597
BstDEI CTNAG 2 cut(s) 117, 487
BstF5I GGATG 2 cut(s) 260, 356
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 450, 455, 464
BstNI CCWGG 1 cut(s) 573
BstNSI RCATGY 2 cut(s) 228, 514
BstSCI CCNGG 1 cut(s) 571
BstSFI CTRYAG 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 536
BstZ17I GTATAC 1 cut(s) 112
BsuRI GGCC 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 468
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 2 cut(s) 260, 356
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 477, 519
Cac8I GCNNGC 3 cut(s) 451, 547, 597
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 244
Csp6I GTAC 4 cut(s) 241, 390, 439, 568
CviAII CATG 2 cut(s) 225, 511
CviQI GTAC 4 cut(s) 241, 390, 439, 568
DdeI CTNAG 2 cut(s) 117, 487
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 70, 598
EarI CTCTTC 2 cut(s) 70, 598
Eco24I GRGCYC 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 571
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 488
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 2 cut(s) 228, 514
FaiI YATR 9 cut(s) 29, 112, 226, 246, 320, 498, 504, 512, 624
FatI CATG 2 cut(s) 224, 510
FblI GTMKAC 2 cut(s) 111, 131
Fnu4HI GCNGC 2 cut(s) 525, 528
FokI GGATG 2 cut(s) 247, 343
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 525, 528
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 525, 528
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 1 cut(s) 249
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 244
Hin1II CATG 2 cut(s) 228, 514
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 2 cut(s) 203, 491
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 112, 132
Hpy188I TCNGA 2 cut(s) 120, 490
Hpy188III TCNNGA 3 cut(s) 84, 217, 590
Hpy8I GTNNAC 2 cut(s) 112, 132
HpyAV CCTTC 3 cut(s) 66, 406, 470
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 9 cut(s) 4, 164, 228, 293, 344, 475, 514, 524, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 450, 455, 464
HpyF3I CTNAG 2 cut(s) 117, 487
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 2 cut(s) 228, 514
KpnI GGTACC 1 cut(s) 571
KroI GCCGGC 1 cut(s) 449
KroNI GCCGGC 1 cut(s) 451
Kzo9I GATC 1 cut(s) 213
LpnPI CCDG 9 cut(s) 224, 230, 461, 463, 558, 559, 585, 603, 626
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 2 cut(s) 13, 445
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 629
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 4 cut(s) 57, 143, 421, 615
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 5 cut(s) 159, 184, 403, 645, 655
MlyI GAGTC 2 cut(s) 197, 500
Mph1103I ATGCAT 1 cut(s) 6
MreI CGCCGGCG 1 cut(s) 449
MroNI GCCGGC 1 cut(s) 449
MslI CAYNNNNRTG 1 cut(s) 627
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 573
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 3 cut(s) 450, 455, 464
NaeI GCCGGC 1 cut(s) 451
NdeII GATC 1 cut(s) 213
NgoMIV GCCGGC 1 cut(s) 449
NlaIII CATG 2 cut(s) 228, 514
NlaIV GGNNCC 1 cut(s) 569
NmuCI GTSAC 1 cut(s) 629
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 228, 514
PctI GAATGC 1 cut(s) 547
PdiI GCCGGC 1 cut(s) 451
PkrI GCNGC 2 cut(s) 526, 529
PleI GAGTC 2 cut(s) 197, 499
PpsI GAGTC 2 cut(s) 197, 499
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 571
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 1 cut(s) 569
PspPI GGNCC 1 cut(s) 415
PspPPI RGGWCCY 1 cut(s) 415
PstI CTGCAG 1 cut(s) 526
RsaI GTAC 4 cut(s) 242, 391, 440, 569
RsaNI GTAC 4 cut(s) 241, 390, 439, 568
RseI CAYNNNNRTG 1 cut(s) 627
SatI GCNGC 2 cut(s) 525, 528
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 2 cut(s) 197, 500
ScrFI CCNGG 1 cut(s) 573
SduI GDGCHC 1 cut(s) 488
SetI ASST 9 cut(s) 42, 356, 386, 417, 469, 569, 577, 597, 636
SfaNI GCATC 2 cut(s) 13, 445
SfcI CTRYAG 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 449
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 2 cut(s) 385, 419
SmoI CTYRAG 2 cut(s) 385, 419
Sse9I AATT 5 cut(s) 159, 184, 403, 645, 655
SsiI CCGC 2 cut(s) 348, 528
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 571
TaiI ACGT 1 cut(s) 636
TasI AATT 5 cut(s) 159, 184, 403, 645, 655
TatI WGTACW 2 cut(s) 240, 389
TauI GCSGC 1 cut(s) 530
TscAI CASTG 2 cut(s) 477, 526
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 629
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 2 cut(s) 477, 526
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 1 cut(s) 403
XbaI TCTAGA 1 cut(s) 83
XceI RCATGY 2 cut(s) 228, 514
XmiI GTMKAC 2 cut(s) 111, 131
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 242
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.