FvH4_1g25470

DNA polymerase alpha chain like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008292
Physical Location & Seq
Reverse (-)
17216487 .. 17221673
5187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25470.t1

Sequence Viewer

Length: 1338 bp
ATGGGTGACAGTTATGTCATTCACACCCCAACTATTTCCAGCAAACAGAGCCCGAAGAAAACAAAGAAGAAAAAGCGAGGGGGAAGTAAGAAGAAAATGACCACTGAGCAGACTATGGCTTTCAAGTCTGTGAGCGAATGGGTTTATTTGGACCAAGCTTCTTCACCGGCTGCCACTGCAGCTTCTTGTGTTGTAGATGATTTCGGGGTTCAGAAGACTCTTGGAAAAGGCGGAGAGAAGGTAGTGTTTGAATTGCATTCACATTCAAAGTGCAGTGATGGGTTTCTTTCACCTTCCAAGTTGGTTGAAAGGGCTCATGGAAATGGGGTGAAAGTTCTTGCTCTGACAGATCATGACACAATGTCTGGCATTCCTGAGGCCCTAGAAGCAGCTCGGAGGTTTGGCATAAAGATAATTCCTGGTGTTGAGATCAGTACAATTTTCCAACCAAGAGGAGAGTCTGAACTGGAGGAACCGGTGCACATCCTTGCATATTACAGCAGCTGTGGGCCAACAAGTTTTGAAGAGCTTGAAAAGTTCTTGTCTAATATAAGGGATGGTCGTTATCTTCGTGCAAAGAATATGGTCTCCAAATTGAACAAGCTTAAGTTTCCTCTTACATGGGAACATGTTGCAAGGGTTGCAGGCAAGGGAGTCGCTCCTGGAAGATTGCATGTGGCCCGAGCCATGGTTGAAGCCGGTTATGTGGAAAATCTGAAACAAGCTTTTGCGCGCTATCTTTTTGATGGTGGACCTGCATACTCCACGGGAAGTGAACCTCCGACAGAGGATGCAATAAAAATGATTCGTGATACAGGAGGTGTGGCTGTGCTAGCTCATCCTTGGGCATTGAAAAATACAGTTGCCATCATCAGAAGTTTGAAAGAAGCTGGTCTTCATGGGATGGAAGTTTACAGATGTGATGGAAAACTGACTGCATACAGTGATCTGGCTGATACTTATGGTCTTCTGAAGCTAGGAGGGTCAGATTACCATGGCAGAGGTGGGCATAGTGAGTCCGAGCTGGGTAGTGTGAACCTTCCAGTTTTGGTTTTGCGCGACTTTCTTAAAGTAGGTCGACCAATTTGGTGTGGTGCCATTGGTCACATATTAGAGAACTATGCTGATGAACCTTCAGATTCGAATCTAGCAAGTATTACAAGGTTTGGGAAGGGAGGTTCCTCCTTAAGCTGCGGCAAGGAGATGATTGATCGTTGCTTGTTTTCATGGTTGACAAATGAAGAGAGACAAAATGCACTTTTTGAGGCCATTAAAGTGAAGCTTTCTCGTATTTCAATTAATAAGGGAGGAAACCAGGTTGCTATAGAAAGTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHP

Protein Analysis

446

Amino Acids

48.44

Weight (kDa)

8.93

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHP PF02811 84 - 163 2.8e-14 PHP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1336
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 763
AccB1I GGYRCC 1 cut(s) 1094
AccI GTMKAC 1 cut(s) 1078
AccII CGCG 2 cut(s) 733, 1059
AciI CCGC 2 cut(s) 231, 1194
AcuI CTGAAG 2 cut(s) 992, 1119
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 688
AflII CTTAAG 2 cut(s) 605, 1186
AflIII ACRYGT 1 cut(s) 628
AgeI ACCGGT 1 cut(s) 475
AhdI GACNNNNNGTC 1 cut(s) 361
AjnI CCWGG 3 cut(s) 418, 661, 1314
AloI GAACNNNNNNTCC 2 cut(s) 1162, 1194
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 2 cut(s) 592, 1240
Alw44I GTGCAC 1 cut(s) 479
AlwNI CAGNNNCTG 1 cut(s) 504
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 4 cut(s) 378, 509, 678, 1266
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 5 cut(s) 170, 179, 389, 501, 1191
AseI ATTAAT 1 cut(s) 1299
AsiGI ACCGGT 1 cut(s) 475
AspLEI GCGC 3 cut(s) 733, 735, 1059
AspS9I GGNCC 5 cut(s) 151, 379, 509, 679, 752
AsuHPI GGTGA 4 cut(s) 17, 156, 282, 340
AsuII TTCGAA 1 cut(s) 1142
AsuNHI GCTAGC 1 cut(s) 832
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 2 cut(s) 151, 752
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 483
BanI GGYRCC 1 cut(s) 1094
BanII GRGCYC 2 cut(s) 53, 316
BbsI GAAGAC 3 cut(s) 221, 887, 959
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 5 cut(s) 157, 191, 401, 513, 1178
BccI CCATC 6 cut(s) 272, 551, 740, 875, 898, 917
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BciT130I CCWGG 3 cut(s) 420, 663, 1316
BcoDI GTCTC 2 cut(s) 592, 1240
BfaI CTAG 4 cut(s) 383, 833, 977, 1148
BfmI CTRYAG 2 cut(s) 177, 1323
BfrI CTTAAG 2 cut(s) 605, 1186
BfuAI ACCTGC 1 cut(s) 763
BisI GCNGC 6 cut(s) 171, 180, 390, 502, 1192, 1195
BlsI GCNGC 6 cut(s) 172, 181, 391, 503, 1193, 1196
Bme1390I CCNGG 3 cut(s) 420, 663, 1316
Bme18I GGWCC 2 cut(s) 151, 752
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 5 cut(s) 151, 379, 509, 679, 752
BmiI GGNNCC 3 cut(s) 474, 1096, 1180
BmrFI CCNGG 3 cut(s) 420, 663, 1316
BmsI GCATC 1 cut(s) 781
BmtI GCTAGC 1 cut(s) 836
BpiI GAAGAC 3 cut(s) 221, 887, 959
BpmI CTGGAG 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 1142
BsaBI GATNNNNATC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 4 cut(s) 687, 765, 842, 994
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse118I RCCGGY 3 cut(s) 166, 475, 698
Bse1I ACTGG 2 cut(s) 471, 1043
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 3 cut(s) 420, 663, 1316
BseDI CCNNGG 4 cut(s) 687, 765, 842, 994
BseGI GGATG 5 cut(s) 483, 562, 796, 838, 909
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 96, 366
BseNI ACTGG 2 cut(s) 471, 1043
BsePI GCGCGC 1 cut(s) 731
BseRI GAGGAG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 5 cut(s) 157, 191, 401, 513, 1178
BseYI CCCAGC 1 cut(s) 1024
BsgI GTGCAG 1 cut(s) 292
Bsh1236I CGCG 2 cut(s) 733, 1059
BshFI GGCC 4 cut(s) 380, 511, 680, 1268
BshNI GGYRCC 1 cut(s) 1094
BshTI ACCGGT 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 3 cut(s) 167, 476, 699
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 2 cut(s) 592, 1240
BsmI GAATGC 2 cut(s) 256, 369
BsnI GGCC 4 cut(s) 380, 511, 680, 1268
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 1 cut(s) 681
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 3 cut(s) 53, 316, 483
Bsp143I GATC 4 cut(s) 349, 429, 946, 1210
Bsp19I CCATGG 2 cut(s) 687, 994
BspACI CCGC 2 cut(s) 231, 1194
BspANI GGCC 4 cut(s) 380, 511, 680, 1268
BspCNI CTCAG 2 cut(s) 97, 367
BspFNI CGCG 2 cut(s) 733, 1059
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 474, 1096, 1180
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 763
BspOI GCTAGC 1 cut(s) 836
BspQI GCTCTTC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 1142
BspT107I GGYRCC 1 cut(s) 1094
BspTI CTTAAG 2 cut(s) 605, 1186
BspTNI GGTCTC 1 cut(s) 592
BsrFI RCCGGY 3 cut(s) 166, 475, 698
BsrI ACTGG 2 cut(s) 471, 1043
BssAI RCCGGY 3 cut(s) 166, 475, 698
BssECI CCNNGG 4 cut(s) 687, 765, 842, 994
BssHII GCGCGC 1 cut(s) 731
BssMI GATC 4 cut(s) 349, 429, 946, 1210
BssT1I CCWWGG 3 cut(s) 687, 842, 994
Bst2UI CCWGG 3 cut(s) 420, 663, 1316
Bst4CI ACNGT 3 cut(s) 11, 862, 944
Bst6I CTCTTC 2 cut(s) 519, 1236
BstAFI CTTAAG 2 cut(s) 605, 1186
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 3 cut(s) 646, 733, 834
BstDEI CTNAG 2 cut(s) 105, 375
BstDSI CCRYGG 3 cut(s) 687, 765, 994
BstF5I GGATG 5 cut(s) 483, 562, 796, 838, 909
BstFNI CGCG 2 cut(s) 733, 1059
BstHHI GCGC 3 cut(s) 733, 735, 1059
BstKTI GATC 4 cut(s) 352, 432, 949, 1213
BstMAI GTCTC 2 cut(s) 592, 1240
BstMBI GATC 4 cut(s) 349, 429, 946, 1210
BstMWI GCNNNNNNNGC 6 cut(s) 48, 176, 179, 386, 641, 833
BstNI CCWGG 3 cut(s) 420, 663, 1316
BstNSI RCATGY 2 cut(s) 632, 677
BstSCI CCNGG 3 cut(s) 418, 661, 1314
BstSFI CTRYAG 2 cut(s) 177, 1323
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 2 cut(s) 733, 1059
BstV1I GCAGC 5 cut(s) 157, 191, 401, 513, 1178
BstV2I GAAGAC 3 cut(s) 221, 887, 959
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 4 cut(s) 380, 511, 680, 1268
BtgI CCRYGG 3 cut(s) 687, 765, 994
BtsCI GGATG 5 cut(s) 483, 562, 796, 838, 909
BtsI GCAGTG 2 cut(s) 174, 280
BtsIMutI CAGTG 4 cut(s) 102, 174, 280, 949
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 3 cut(s) 646, 733, 834
CaiI CAGNNNCTG 1 cut(s) 504
CciI TCATGA 1 cut(s) 352
CfoI GCGC 3 cut(s) 733, 735, 1059
Cfr10I RCCGGY 3 cut(s) 166, 475, 698
Cfr13I GGNCC 5 cut(s) 151, 379, 509, 679, 752
CsiI ACCWGGT 1 cut(s) 1314
Csp6I GTAC 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 475
CviAII CATG 9 cut(s) 317, 353, 621, 629, 674, 688, 899, 995, 1227
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 2 cut(s) 105, 375
DpnI GATC 4 cut(s) 351, 431, 948, 1212
DpnII GATC 4 cut(s) 349, 429, 946, 1210
DrdI GACNNNNNNGTC 1 cut(s) 14
DriI GACNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 519, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 2 cut(s) 519, 1236
EciI GGCGGA 1 cut(s) 246
Eco130I CCWWGG 3 cut(s) 687, 842, 994
Eco24I GRGCYC 2 cut(s) 53, 316
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 2 cut(s) 151, 752
Eco57I CTGAAG 2 cut(s) 992, 1119
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 3 cut(s) 418, 661, 1314
EcoT14I CCWWGG 3 cut(s) 687, 842, 994
EcoT38I GRGCYC 2 cut(s) 53, 316
ErhI CCWWGG 3 cut(s) 687, 842, 994
FaeI CATG 9 cut(s) 320, 356, 624, 632, 677, 691, 902, 998, 1230
FalI AAGNNNNNCTT 4 cut(s) 879, 911, 1266, 1298
FatI CATG 9 cut(s) 316, 352, 620, 628, 673, 687, 898, 994, 1226
FblI GTMKAC 1 cut(s) 1078
Fnu4HI GCNGC 6 cut(s) 171, 180, 390, 502, 1192, 1195
FokI GGATG 5 cut(s) 470, 569, 803, 825, 916
FriOI GRGCYC 2 cut(s) 53, 316
Fsp4HI GCNGC 6 cut(s) 171, 180, 390, 502, 1192, 1195
FspBI CTAG 4 cut(s) 383, 833, 977, 1148
GlaI GCGC 3 cut(s) 732, 734, 1058
GluI GCNGC 6 cut(s) 171, 180, 390, 502, 1192, 1195
GsaI CCCAGC 1 cut(s) 1028
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 4 cut(s) 380, 511, 680, 1268
HapII CCGG 3 cut(s) 167, 476, 699
HhaI GCGC 3 cut(s) 733, 735, 1059
Hin1II CATG 9 cut(s) 320, 356, 624, 632, 677, 691, 902, 998, 1230
Hin6I GCGC 3 cut(s) 731, 733, 1057
HinP1I GCGC 3 cut(s) 731, 733, 1057
HincII GTYRAC 2 cut(s) 1079, 1233
HindII GTYRAC 2 cut(s) 1079, 1233
HindIII AAGCTT 4 cut(s) 156, 602, 723, 1280
HinfI GANTC 7 cut(s) 217, 458, 654, 805, 1016, 1139, 1144
HpaII CCGG 3 cut(s) 167, 476, 699
HphI GGTGA 4 cut(s) 17, 156, 282, 340
Hpy166II GTNNAC 7 cut(s) 481, 752, 776, 913, 1036, 1079, 1233
Hpy188III TCNNGA 3 cut(s) 353, 374, 809
Hpy8I GTNNAC 7 cut(s) 481, 752, 776, 913, 1036, 1079, 1233
HpyAV CCTTC 5 cut(s) 232, 303, 1049, 1143, 1165
HpyCH4III ACNGT 3 cut(s) 11, 862, 944
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 176, 179, 386, 641, 833
HpyF3I CTNAG 2 cut(s) 105, 375
Hsp92II CATG 9 cut(s) 320, 356, 624, 632, 677, 691, 902, 998, 1230
HspAI GCGC 3 cut(s) 731, 733, 1057
Kzo9I GATC 4 cut(s) 349, 429, 946, 1210
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 1 cut(s) 664
Lsp1109I GCAGC 5 cut(s) 157, 191, 401, 513, 1178
LweI GCATC 1 cut(s) 781
MabI ACCWGGT 1 cut(s) 1314
MaeI CTAG 4 cut(s) 383, 833, 977, 1148
MaeIII GTNAC 2 cut(s) 5, 1103
MalI GATC 4 cut(s) 351, 431, 948, 1212
MboI GATC 4 cut(s) 349, 429, 946, 1210
MhlI GDGCHC 3 cut(s) 53, 316, 483
MluCI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
MlyI GAGTC 4 cut(s) 211, 467, 663, 1025
MmeI TCCRAC 2 cut(s) 469, 806
MseI TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
MslI CAYNNNNRTG 2 cut(s) 321, 1274
MspA1I CMGCKG 1 cut(s) 504
MspCI CTTAAG 2 cut(s) 605, 1186
MspI CCGG 3 cut(s) 167, 476, 699
MspR9I CCNGG 3 cut(s) 420, 663, 1316
Mva1269I GAATGC 2 cut(s) 256, 369
MvaI CCWGG 3 cut(s) 420, 663, 1316
MvnI CGCG 2 cut(s) 733, 1059
MwoI GCNNNNNNNGC 6 cut(s) 48, 176, 179, 386, 641, 833
NcoI CCATGG 2 cut(s) 687, 994
NdeII GATC 4 cut(s) 349, 429, 946, 1210
NheI GCTAGC 1 cut(s) 832
NlaIII CATG 9 cut(s) 320, 356, 624, 632, 677, 691, 902, 998, 1230
NlaIV GGNNCC 3 cut(s) 474, 1096, 1180
NmuCI GTSAC 2 cut(s) 5, 1103
NspI RCATGY 2 cut(s) 632, 677
NspV TTCGAA 1 cut(s) 1142
PagI TCATGA 1 cut(s) 352
PauI GCGCGC 1 cut(s) 731
PciI ACATGT 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 2 cut(s) 256, 369
PfeI GAWTC 3 cut(s) 805, 1139, 1144
PfoI TCCNGGA 1 cut(s) 661
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 6 cut(s) 172, 181, 391, 503, 1193, 1196
PleI GAGTC 4 cut(s) 211, 466, 662, 1024
PpsI GAGTC 4 cut(s) 211, 466, 662, 1024
PscI ACATGT 1 cut(s) 628
PshBI ATTAAT 1 cut(s) 1299
PsiI TTATAA 1 cut(s) 1336
Psp6I CCWGG 3 cut(s) 418, 661, 1314
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 3 cut(s) 418, 661, 1314
PspN4I GGNNCC 3 cut(s) 474, 1096, 1180
PspPI GGNCC 5 cut(s) 151, 379, 509, 679, 752
PstI CTGCAG 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 504
PteI GCGCGC 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 504
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 2 cut(s) 321, 1274
SalI GTCGAC 1 cut(s) 1077
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
SatI GCNGC 6 cut(s) 171, 180, 390, 502, 1192, 1195
Sau3AI GATC 4 cut(s) 349, 429, 946, 1210
Sau96I GGNCC 5 cut(s) 151, 379, 509, 679, 752
SchI GAGTC 4 cut(s) 211, 467, 663, 1025
ScrFI CCNGG 3 cut(s) 420, 663, 1316
SduI GDGCHC 3 cut(s) 53, 316, 483
SexAI ACCWGGT 1 cut(s) 1314
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 2 cut(s) 177, 1323
SfuI TTCGAA 1 cut(s) 1142
SinI GGWCC 2 cut(s) 151, 752
SmiMI CAYNNNNRTG 2 cut(s) 321, 1274
SmlI CTYRAG 2 cut(s) 605, 1186
SmoI CTYRAG 2 cut(s) 605, 1186
Sse9I AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
SsiI CCGC 2 cut(s) 231, 1194
SspMI CTAG 4 cut(s) 383, 833, 977, 1148
StyD4I CCNGG 3 cut(s) 418, 661, 1314
StyI CCWWGG 3 cut(s) 687, 842, 994
TaaI ACNGT 3 cut(s) 11, 862, 944
TaqI TCGA 2 cut(s) 1078, 1142
TasI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 1197
TfiI GAWTC 3 cut(s) 805, 1139, 1144
Tru1I TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
Tru9I TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
TscAI CASTG 4 cut(s) 109, 181, 280, 949
TseFI GTSAC 2 cut(s) 5, 1103
TseI GCWGC 5 cut(s) 170, 179, 389, 501, 1191
Tsp45I GTSAC 2 cut(s) 5, 1103
TspDTI ATGAA 4 cut(s) 887, 1143, 1215, 1254
TspRI CASTG 4 cut(s) 109, 181, 280, 949
Vha464I CTTAAG 2 cut(s) 605, 1186
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 2 cut(s) 151, 752
VspI ATTAAT 1 cut(s) 1299
XceI RCATGY 2 cut(s) 632, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 1078
XspI CTAG 4 cut(s) 383, 833, 977, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.