Rw2G024480

DNA polymerase alpha chain like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008292
Physical Location & Seq
Reverse (-)
36131762 .. 36137086
5325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024480.1

Sequence Viewer

Length: 1338 bp
ATGGGCGACAGTTATGTCATTCACACCCCAAGTATTTCCAGCAAACAGAGCCCTAAGAAAACAAAGAAGAAAAAGCGTGGGGGAAGTAAGAAGAAAATGACCACTGAGCAGACTCTGGCTTTCAAGTCTGTGAGTGAATGGGTTTATTTGGATCAAGCTTCTTCACCGGCTTCCACTGCAGCTTCTGGTGTTGTGGATGATTTTGGGGTTCAGAAGACTATTGGAAGAGGTGGAGAGAAGGTAGTGTTTGAATTGCATTCACATTCAAAATGCAGTGATGGCTTTCTTTCGCCTTCCAAGTTGGTGGAAAGGGCTCATGGAAATGGGGTGAAAGTTCTTGCTCTGACAGATCATGACACAATGTCTGGCATCCCTGAGGCCCTAGAAGCAGCTCGTAGATTTGGCATGAAGATAATTCCTGGTGTTGAAATCAGTACAATTTTCCAACCAAGAGGAGAGTCTGAACCGGAGGAACCAGTGCACATCCTTGCATATTACAGCAGCTGCGGGCCAACAAGTTTTGAAGAGCTTGAAAAGTTCTTGTCTAATATAAGGGATGGTCGTTATCTTCGTGCAAAGAATATGGTCTCCAAATTGAACAAGCTTAAGTTTCCTCTTACGTGGGAGCATGTTGCAAGGGTTGCAGGCAAGGGAGTCGCTCCTGGGAGATTGCATGTGGCCCGAGCCATGGTTGAAGCCGGTTATGTGGAAAATCTGAAACAAGCCTTTGCCCGCTATCTTTTCGATGGTGGACCTGCATATGCTACGGGAAGTGAACCGCCGACAGAGGATGCAATAAAAATGATATGTGATACGGGAGGTGTGGCTGTGCTAGCTCATCCATGGGCATTGAAAAATACAGTTGCCATCATCAGAAGTTTGAAAGAAGCTGGTCTTCATGGGATGGAAGTTTACAGAAGTGATGGAAAACTGACTGCATACAGTGATCTAGCTGATACTTATGGTCTTCTGAAGCTAGGAGGGTCAGATTACCATGGCCGAGGTGGACAGGGTGAGTCTGAGCTGGGTAGTGTGAACCTTCCGGTTTTGGTTTTGCGTGACTTTCTTAAAGTCGCTCGACCAATTTGGTGTGGTGCCATTAGTCACATATTAGAGAACTATGCTGACGAACCTTCAGATTCAAATCTAGCAAGGATTACAAGGTTTGGGAAGGGTGGTTCCCCTGTAAGCTGCGGCAAGGACATGATTGATCGTTGCTTGTTTTTGTGGTTGACAAATGAAGAGAGGCAAAATGCAGTTTTTGAGGCCATTAAACTGAAGCTTTCTCGTATTTCAATTAATCACGGAGGAAATCAGGTTGCTATAGAGAGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHP

Protein Analysis

445

Amino Acids

48.43

Weight (kDa)

8.97

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHP PF02811 84 - 161 1.4e-13 PHP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 763
AccB1I GGYRCC 1 cut(s) 1094
AciI CCGC 4 cut(s) 507, 733, 779, 1194
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 997
AcuI CTGAAG 3 cut(s) 992, 1119, 1298
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 688
AflII CTTAAG 1 cut(s) 605
AhdI GACNNNNNGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 418, 661
AloI GAACNNNNNNTCC 2 cut(s) 1162, 1194
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 592
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 159
AlwNI CAGNNNCTG 3 cut(s) 115, 185, 504
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 5 cut(s) 378, 509, 678, 997, 1266
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 5 cut(s) 179, 389, 501, 504, 1191
AseI ATTAAT 1 cut(s) 1299
AspS9I GGNCC 4 cut(s) 379, 509, 679, 752
AsuHPI GGTGA 3 cut(s) 156, 340, 1025
AsuNHI GCTAGC 1 cut(s) 832
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 752
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 483
BanI GGYRCC 1 cut(s) 1094
BanII GRGCYC 2 cut(s) 53, 316
BbsI GAAGAC 3 cut(s) 221, 887, 959
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 5 cut(s) 191, 401, 491, 513, 1178
BccI CCATC 6 cut(s) 272, 551, 740, 875, 898, 917
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BciT130I CCWGG 2 cut(s) 420, 663
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 5 cut(s) 383, 833, 950, 977, 1148
BfmI CTRYAG 2 cut(s) 177, 1323
BfrI CTTAAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 763
BisI GCNGC 6 cut(s) 180, 390, 502, 505, 1192, 1195
BlsI GCNGC 6 cut(s) 181, 391, 503, 506, 1193, 1196
Bme1390I CCNGG 2 cut(s) 420, 663
Bme18I GGWCC 1 cut(s) 752
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 4 cut(s) 379, 509, 679, 752
BmiI GGNNCC 3 cut(s) 474, 1096, 1180
BmrFI CCNGG 2 cut(s) 420, 663
BmsI GCATC 2 cut(s) 378, 781
BmtI GCTAGC 1 cut(s) 836
BpiI GAAGAC 3 cut(s) 221, 887, 959
BsaAI YACGTR 1 cut(s) 621
BsaBI GATNNNNATC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 5 cut(s) 662, 687, 842, 994, 1000
BsaWI WCCGGW 2 cut(s) 466, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse118I RCCGGY 2 cut(s) 166, 698
Bse1I ACTGG 1 cut(s) 476
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 420, 663
BseDI CCNNGG 5 cut(s) 662, 687, 842, 994, 1000
BseGI GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 3 cut(s) 96, 366, 1011
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 5 cut(s) 191, 401, 491, 513, 1178
BseYI CCCAGC 1 cut(s) 1024
BshFI GGCC 5 cut(s) 380, 511, 680, 999, 1268
BshNI GGYRCC 1 cut(s) 1094
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 4 cut(s) 167, 467, 699, 1043
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 1 cut(s) 592
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 5 cut(s) 380, 511, 680, 999, 1268
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 3 cut(s) 53, 316, 483
Bsp143I GATC 4 cut(s) 151, 349, 946, 1210
Bsp19I CCATGG 3 cut(s) 687, 842, 994
BspACI CCGC 4 cut(s) 507, 733, 779, 1194
BspANI GGCC 5 cut(s) 380, 511, 680, 999, 1268
BspCNI CTCAG 3 cut(s) 97, 367, 1012
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 474, 1096, 1180
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 763
BspOI GCTAGC 1 cut(s) 836
BspPI GGATC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 1094
BspTI CTTAAG 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 592
BsrFI RCCGGY 2 cut(s) 166, 698
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 2 cut(s) 166, 698
BssECI CCNNGG 5 cut(s) 662, 687, 842, 994, 1000
BssMI GATC 4 cut(s) 151, 349, 946, 1210
BssT1I CCWWGG 3 cut(s) 687, 842, 994
Bst2UI CCWGG 2 cut(s) 420, 663
Bst4CI ACNGT 3 cut(s) 11, 862, 944
Bst6I CTCTTC 3 cut(s) 220, 519, 1236
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 621
BstC8I GCNNGC 4 cut(s) 509, 646, 733, 834
BstDEI CTNAG 4 cut(s) 54, 105, 375, 1020
BstDSI CCRYGG 3 cut(s) 687, 842, 994
BstF5I GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BstKTI GATC 4 cut(s) 154, 352, 949, 1213
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 4 cut(s) 151, 349, 946, 1210
BstMWI GCNNNNNNNGC 6 cut(s) 48, 176, 279, 386, 641, 833
BstNI CCWGG 2 cut(s) 420, 663
BstNSI RCATGY 2 cut(s) 632, 677
BstSCI CCNGG 2 cut(s) 418, 661
BstSFI CTRYAG 2 cut(s) 177, 1323
BstSLI GKGCMC 1 cut(s) 483
BstV1I GCAGC 5 cut(s) 191, 401, 491, 513, 1178
BstV2I GAAGAC 3 cut(s) 221, 887, 959
BstXI CCANNNNNNTGG 1 cut(s) 304
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 5 cut(s) 380, 511, 680, 999, 1268
BtgI CCRYGG 3 cut(s) 687, 842, 994
BtsCI GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BtsI GCAGTG 2 cut(s) 174, 280
BtsIMutI CAGTG 5 cut(s) 102, 174, 280, 483, 949
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 4 cut(s) 509, 646, 733, 834
CaiI CAGNNNCTG 3 cut(s) 115, 185, 504
CciI TCATGA 1 cut(s) 352
Cfr10I RCCGGY 2 cut(s) 166, 698
Cfr13I GGNCC 4 cut(s) 379, 509, 679, 752
Csp6I GTAC 1 cut(s) 435
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 4 cut(s) 54, 105, 375, 1020
DpnI GATC 4 cut(s) 153, 351, 948, 1212
DpnII GATC 4 cut(s) 151, 349, 946, 1210
DrdI GACNNNNNNGTC 1 cut(s) 14
DriI GACNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 3 cut(s) 220, 519, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 3 cut(s) 220, 519, 1236
Eco130I CCWWGG 3 cut(s) 687, 842, 994
Eco24I GRGCYC 2 cut(s) 53, 316
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 752
Eco57I CTGAAG 3 cut(s) 992, 1119, 1298
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 418, 661
EcoT14I CCWWGG 3 cut(s) 687, 842, 994
EcoT38I GRGCYC 2 cut(s) 53, 316
ErhI CCWWGG 3 cut(s) 687, 842, 994
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FauI CCCGC 2 cut(s) 500, 740
FauNDI CATATG 1 cut(s) 760
Fnu4HI GCNGC 6 cut(s) 180, 390, 502, 505, 1192, 1195
FokI GGATG 7 cut(s) 209, 356, 470, 569, 803, 825, 916
FriOI GRGCYC 2 cut(s) 53, 316
Fsp4HI GCNGC 6 cut(s) 180, 390, 502, 505, 1192, 1195
FspBI CTAG 5 cut(s) 383, 833, 950, 977, 1148
GluI GCNGC 6 cut(s) 180, 390, 502, 505, 1192, 1195
GsaI CCCAGC 1 cut(s) 1028
HaeIII GGCC 5 cut(s) 380, 511, 680, 999, 1268
HapII CCGG 4 cut(s) 167, 467, 699, 1043
HincII GTYRAC 1 cut(s) 1233
HindII GTYRAC 1 cut(s) 1233
HindIII AAGCTT 3 cut(s) 156, 602, 1280
HinfI GANTC 5 cut(s) 112, 458, 654, 1016, 1139
HpaII CCGG 4 cut(s) 167, 467, 699, 1043
HphI GGTGA 3 cut(s) 156, 340, 1025
Hpy166II GTNNAC 7 cut(s) 481, 752, 776, 913, 1007, 1036, 1233
Hpy188I TCNGA 9 cut(s) 213, 345, 463, 717, 875, 972, 988, 1021, 1138
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 7 cut(s) 481, 752, 776, 913, 1007, 1036, 1233
HpyAV CCTTC 5 cut(s) 232, 303, 1049, 1143, 1165
HpyCH4III ACNGT 3 cut(s) 11, 862, 944
HpyCH4IV ACGT 1 cut(s) 620
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 176, 279, 386, 641, 833
HpyF3I CTNAG 4 cut(s) 54, 105, 375, 1020
HpySE526I ACGT 1 cut(s) 620
Kzo9I GATC 4 cut(s) 151, 349, 946, 1210
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 2 cut(s) 625, 664
Lsp1109I GCAGC 5 cut(s) 191, 401, 491, 513, 1178
LweI GCATC 2 cut(s) 378, 781
MaeI CTAG 5 cut(s) 383, 833, 950, 977, 1148
MaeII ACGT 1 cut(s) 620
MaeIII GTNAC 2 cut(s) 1058, 1103
MalI GATC 4 cut(s) 153, 351, 948, 1212
MboI GATC 4 cut(s) 151, 349, 946, 1210
MhlI GDGCHC 3 cut(s) 53, 316, 483
MluCI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
MlyI GAGTC 4 cut(s) 106, 467, 663, 1025
MmeI TCCRAC 1 cut(s) 469
MseI TTAA 4 cut(s) 606, 1068, 1272, 1299
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 504
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 4 cut(s) 167, 467, 699, 1043
MspR9I CCNGG 2 cut(s) 420, 663
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 2 cut(s) 420, 663
MwoI GCNNNNNNNGC 6 cut(s) 48, 176, 279, 386, 641, 833
NcoI CCATGG 3 cut(s) 687, 842, 994
NdeI CATATG 1 cut(s) 760
NdeII GATC 4 cut(s) 151, 349, 946, 1210
NheI GCTAGC 1 cut(s) 832
NlaIV GGNNCC 3 cut(s) 474, 1096, 1180
NmeAIII GCCGAG 1 cut(s) 1025
NmuCI GTSAC 2 cut(s) 1058, 1103
NspI RCATGY 2 cut(s) 632, 677
PagI TCATGA 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 1139
PkrI GCNGC 6 cut(s) 181, 391, 503, 506, 1193, 1196
PleI GAGTC 4 cut(s) 106, 466, 662, 1024
PpsI GAGTC 4 cut(s) 106, 466, 662, 1024
Ppu21I YACGTR 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 1299
Psp6I CCWGG 2 cut(s) 418, 661
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 2 cut(s) 418, 661
PspN4I GGNNCC 3 cut(s) 474, 1096, 1180
PspPI GGNCC 4 cut(s) 379, 509, 679, 752
PstI CTGCAG 1 cut(s) 181
PstNI CAGNNNCTG 3 cut(s) 115, 185, 504
PvuII CAGCTG 1 cut(s) 504
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 4 cut(s) 606, 1068, 1272, 1299
SatI GCNGC 6 cut(s) 180, 390, 502, 505, 1192, 1195
Sau3AI GATC 4 cut(s) 151, 349, 946, 1210
Sau96I GGNCC 4 cut(s) 379, 509, 679, 752
SchI GAGTC 4 cut(s) 106, 467, 663, 1025
ScrFI CCNGG 2 cut(s) 420, 663
SduI GDGCHC 3 cut(s) 53, 316, 483
SfaNI GCATC 2 cut(s) 378, 781
SfcI CTRYAG 2 cut(s) 177, 1323
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
Sse9I AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
SsiI CCGC 4 cut(s) 507, 733, 779, 1194
SspMI CTAG 5 cut(s) 383, 833, 950, 977, 1148
StyD4I CCNGG 2 cut(s) 418, 661
StyI CCWWGG 3 cut(s) 687, 842, 994
TaaI ACNGT 3 cut(s) 11, 862, 944
TaiI ACGT 1 cut(s) 623
TaqI TCGA 2 cut(s) 744, 1078
TasI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 1197
TfiI GAWTC 1 cut(s) 1139
Tru1I TTAA 4 cut(s) 606, 1068, 1272, 1299
Tru9I TTAA 4 cut(s) 606, 1068, 1272, 1299
TscAI CASTG 5 cut(s) 109, 181, 280, 483, 949
TseFI GTSAC 2 cut(s) 1058, 1103
TseI GCWGC 5 cut(s) 179, 389, 501, 504, 1191
Tsp45I GTSAC 2 cut(s) 1058, 1103
TspDTI ATGAA 3 cut(s) 422, 887, 1254
TspGWI ACGGA 1 cut(s) 1320
TspRI CASTG 5 cut(s) 109, 181, 280, 483, 949
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 1299
XceI RCATGY 2 cut(s) 632, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XspI CTAG 5 cut(s) 383, 833, 950, 977, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.