pycom10g03690

DNA polymerase alpha chain like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008292
Physical Location & Seq
Forward (+)
3993840 .. 3998615
4776 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g03690.1

Sequence Viewer

Length: 1365 bp
ATGAAAAAGTCATGGCCACCGTATGTATCCGTATTTCGTGCCGACTCCGTGATGGATGCCACAAACAACTCGAGCAGCCCAAAGAAAACAGCGAGGAAGAAGAAGAAGCGAGGTGGGAGCGGGAGCAAGAAGAAGATGACCGCCGAGCAGACTTTAGCTTTCAAGTCTGTGAGTGAATGGGTTTTCTTGGAACAGCACCAAGCTTCTTCCGCTGCAGCGTCGGATGATTTTGGGGTTTCGTTGGTGCAAAGGAGCATTCCAAACAAGCTACTCTTTGAATTGCATTCCCATTCAAATTGCAGTGATGGGTTTCTTTCGCCTTCCAAGCTGGTCGAGAGGGCTCACGAAAATGGGGTGAAAGTTCTTGCTCTGACAGATCATGACACAATGTCTGGCATCCCCGAGGCCCTAGAAGCAGCTCGGAGACATCGTATTAAGATAATTCCAGGTGTTGAAATAAGTACAAAATTCTACTCAAGAGGAGAGTCTGCACCAGAGGAGCCAGTTCACATCCTTGCATACTACAGCAGCTGTGGACCAACAAGTTTCGAAGATCTGGAGAAGTTCTTGTCAAATATAAGGGATGGTCGTTATCTTCGTGCAAAAAATATGGTCTTGAAATTGAACAAGCTCAAGTTCCCTCTTAAATGGGAGCATGTCGCAAAGATTGCAGGCAAGGGAGTTGCTCCTGGGAGAGTGCATGTGGCCCGTGCGATGGTTGAAGCCGGCTATGTGGAAAATTTGAAACAAGCTTTTGCCCGCTATCTTTACGATGGTGGACCAGCATACTCTACGGGAAGTGAACCTCTGGCAGAAGAAGCAATAAAACTGATATGTGACACGGGAGGTGTAGCTGTGCTAGCTCATCCTTGGTCATTGAAAAATCCAGTTGCCATCGTCAAAAGTTTGAAAGAAGCTGGTCTTCATGGGATCGAAGTTTACAGAAGTGATGGAAAACTAGCTGTGTACAGTGATCTTGCTGATACTTATGATCTGCTGAAGCTTGGAGGGTCAGATTACCATGGGAGAGGCGGGCATGGCGAGTCCGAGGTGGGTAGTGTGAATCTTCCAGTATTGGTTTTGCGTGACTTTCTTAAAGTAGCTCGACCAATTTGGTGCTGTTCCATTCGGCGCATTTTAGAAAATTATGCCGAGGAGCCTTCGGATTTGAATCTAGCTAGGGTTACGAGGTTTGAGAAAGGAGGTTCCCGCTTAAGCTGCGGCAAGGACCTGATTGATCGTTGCTTCAATTTGTGGTTGACAAGTGAAGAGAGGCAGAATGCAGTTTTTGAGGCCATTAGATTGAAGCTTTCCCATGTTTCAATTAACAAGGGAGGTATCCAGGTTCTTATAGAGAGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHP

Protein Analysis

455

Amino Acids

50.07

Weight (kDa)

9.18

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHP PF02811 93 - 163 3.6e-15 PHP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 7 cut(s) 120, 141, 210, 760, 1032, 1210, 1221
AclWI GGATC 1 cut(s) 938
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 2 cut(s) 467, 739
AcuI CTGAAG 1 cut(s) 1019
AfaI GTAC 3 cut(s) 463, 968, 1360
AfiI CCNNNNNNNGG 1 cut(s) 715
AflII CTTAAG 1 cut(s) 1213
AhdI GACNNNNNGTC 1 cut(s) 388
AjnI CCWGG 3 cut(s) 445, 688, 1341
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 938
AlwNI CAGNNNCTG 1 cut(s) 531
Ama87I CYCGRG 2 cut(s) 70, 401
AoxI GGCC 4 cut(s) 14, 405, 705, 1293
ApeKI GCWGC 6 cut(s) 75, 212, 215, 416, 528, 1218
ApoI RAATTY 2 cut(s) 467, 739
AspLEI GCGC 1 cut(s) 1134
AspS9I GGNCC 5 cut(s) 406, 536, 706, 779, 1228
AsuHPI GGTGA 1 cut(s) 367
AsuII TTCGAA 1 cut(s) 549
AsuNHI GCTAGC 1 cut(s) 859
AvaI CYCGRG 2 cut(s) 70, 401
AvaII GGWCC 3 cut(s) 536, 779, 1228
BalI TGGCCA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 914
BbvI GCAGC 6 cut(s) 87, 199, 227, 428, 540, 1205
BccI CCATC 7 cut(s) 46, 299, 578, 709, 767, 902, 944
BciT130I CCWGG 3 cut(s) 447, 690, 1343
BciVI GTATCC 2 cut(s) 37, 1349
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 5 cut(s) 410, 860, 959, 1175, 1179
BfmI CTRYAG 2 cut(s) 213, 523
BfrI CTTAAG 1 cut(s) 1213
BfuI GTATCC 2 cut(s) 37, 1349
BglII AGATCT 1 cut(s) 553
BisI GCNGC 7 cut(s) 76, 213, 216, 417, 529, 1219, 1222
BlsI GCNGC 7 cut(s) 77, 214, 217, 418, 530, 1220, 1223
Bme1390I CCNGG 3 cut(s) 447, 690, 1343
Bme18I GGWCC 3 cut(s) 536, 779, 1228
BmeRI GACNNNNNGTC 1 cut(s) 388
BmeT110I CYCGRG 2 cut(s) 70, 401
BmgT120I GGNCC 5 cut(s) 406, 536, 706, 779, 1228
BmiI GGNNCC 3 cut(s) 501, 1158, 1207
BmrFI CCNGG 3 cut(s) 447, 690, 1343
BmsI GCATC 2 cut(s) 46, 405
BmtI GCTAGC 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 914
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 460, 617
BsaBI GATNNNNATC 1 cut(s) 1170
BsaJI CCNNGG 6 cut(s) 402, 689, 869, 1021, 1047, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 1 cut(s) 715
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 3 cut(s) 503, 887, 1070
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 3 cut(s) 447, 690, 1343
BseDI CCNNGG 6 cut(s) 402, 689, 869, 1021, 1047, 1152
BseGI GGATG 6 cut(s) 61, 229, 396, 510, 589, 865
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 1 cut(s) 715
BseNI ACTGG 3 cut(s) 503, 887, 1070
BseRI GAGGAG 3 cut(s) 495, 512, 1169
BseXI GCAGC 6 cut(s) 87, 199, 227, 428, 540, 1205
BsgI GTGCAG 1 cut(s) 474
BshFI GGCC 4 cut(s) 16, 407, 707, 1295
BsiHKCI CYCGRG 2 cut(s) 70, 401
BsiSI CCGG 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 715
BsmAI GTCTC 1 cut(s) 418
BsmI GAATGC 3 cut(s) 255, 283, 1285
BsnI GGCC 4 cut(s) 16, 407, 707, 1295
BsoBI CYCGRG 2 cut(s) 70, 401
Bsp119I TTCGAA 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 966
Bsp143I GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
Bsp19I CCATGG 1 cut(s) 1021
BspACI CCGC 7 cut(s) 120, 141, 210, 760, 1032, 1210, 1221
BspANI GGCC 4 cut(s) 16, 407, 707, 1295
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 3 cut(s) 501, 1158, 1207
BspMAI CTGCAG 1 cut(s) 217
BspOI GCTAGC 1 cut(s) 863
BspPI GGATC 1 cut(s) 938
BspT104I TTCGAA 1 cut(s) 549
BspTI CTTAAG 1 cut(s) 1213
BsrBI CCGCTC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 725
BsrGI TGTACA 1 cut(s) 966
BsrI ACTGG 3 cut(s) 503, 887, 1070
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 6 cut(s) 402, 689, 869, 1021, 1047, 1152
BssMI GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
BssT1I CCWWGG 2 cut(s) 869, 1021
Bst2UI CCWGG 3 cut(s) 447, 690, 1343
Bst4CI ACNGT 2 cut(s) 21, 971
Bst6I CTCTTC 1 cut(s) 1263
BstAFI CTTAAG 1 cut(s) 1213
BstAPI GCANNNNNTGC 1 cut(s) 668
BstAUI TGTACA 1 cut(s) 966
BstBI TTCGAA 1 cut(s) 549
BstC8I GCNNGC 5 cut(s) 673, 727, 760, 861, 1034
BstDSI CCRYGG 1 cut(s) 1021
BstF5I GGATG 6 cut(s) 61, 229, 396, 510, 589, 865
BstHHI GCGC 1 cut(s) 1134
BstKTI GATC 6 cut(s) 379, 556, 933, 976, 994, 1240
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
BstMWI GCNNNNNNNGC 8 cut(s) 209, 325, 413, 668, 818, 860, 1038, 1218
BstNI CCWGG 3 cut(s) 447, 690, 1343
BstNSI RCATGY 2 cut(s) 659, 704
BstSCI CCNGG 3 cut(s) 445, 688, 1341
BstSFI CTRYAG 2 cut(s) 213, 523
BstV1I GCAGC 6 cut(s) 87, 199, 227, 428, 540, 1205
BstV2I GAAGAC 1 cut(s) 914
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuI GTATCC 2 cut(s) 37, 1349
BsuRI GGCC 4 cut(s) 16, 407, 707, 1295
BtgI CCRYGG 1 cut(s) 1021
BtgZI GCGATG 1 cut(s) 728
BtsCI GGATG 6 cut(s) 61, 229, 396, 510, 589, 865
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 307, 976
Cac8I GCNNGC 5 cut(s) 673, 727, 760, 861, 1034
CaiI CAGNNNCTG 1 cut(s) 531
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 1134
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 5 cut(s) 406, 536, 706, 779, 1228
CseI GACGC 1 cut(s) 207
Csp6I GTAC 3 cut(s) 462, 967, 1359
CviAII CATG 8 cut(s) 12, 380, 656, 701, 926, 1022, 1037, 1316
CviQI GTAC 3 cut(s) 462, 967, 1359
DpnI GATC 6 cut(s) 378, 555, 932, 975, 993, 1239
DpnII GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
DriI GACNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 14
Eam1104I CTCTTC 1 cut(s) 1263
Eam1105I GACNNNNNGTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 1263
Eco130I CCWWGG 2 cut(s) 869, 1021
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 3 cut(s) 536, 779, 1228
Eco57I CTGAAG 1 cut(s) 1019
Eco88I CYCGRG 2 cut(s) 70, 401
EcoO109I RGGNCCY 2 cut(s) 406, 1228
EcoRII CCWGG 3 cut(s) 445, 688, 1341
EcoT14I CCWWGG 2 cut(s) 869, 1021
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 2 cut(s) 869, 1021
FaeI CATG 8 cut(s) 15, 383, 659, 704, 929, 1025, 1040, 1319
FalI AAGNNNNNCTT 4 cut(s) 257, 289, 906, 938
FatI CATG 8 cut(s) 11, 379, 655, 700, 925, 1021, 1036, 1315
FauI CCCGC 4 cut(s) 113, 767, 1025, 1217
Fnu4HI GCNGC 7 cut(s) 76, 213, 216, 417, 529, 1219, 1222
FokI GGATG 6 cut(s) 68, 236, 383, 497, 596, 852
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 7 cut(s) 76, 213, 216, 417, 529, 1219, 1222
FspBI CTAG 5 cut(s) 410, 860, 959, 1175, 1179
GlaI GCGC 1 cut(s) 1133
GluI GCNGC 7 cut(s) 76, 213, 216, 417, 529, 1219, 1222
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 4 cut(s) 16, 407, 707, 1295
HapII CCGG 1 cut(s) 726
HgaI GACGC 1 cut(s) 207
HhaI GCGC 1 cut(s) 1134
Hin1II CATG 8 cut(s) 15, 383, 659, 704, 929, 1025, 1040, 1319
Hin6I GCGC 1 cut(s) 1132
HinP1I GCGC 1 cut(s) 1132
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 4 cut(s) 201, 750, 1001, 1307
HinfI GANTC 5 cut(s) 44, 485, 1043, 1063, 1171
HpaII CCGG 1 cut(s) 726
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 7 cut(s) 508, 536, 779, 803, 940, 967, 1260
Hpy188I TCNGA 6 cut(s) 223, 372, 423, 1015, 1048, 1165
Hpy188III TCNNGA 6 cut(s) 334, 344, 380, 477, 557, 616
Hpy8I GTNNAC 7 cut(s) 508, 536, 779, 803, 940, 967, 1260
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 2 cut(s) 330, 1170
HpyCH4III ACNGT 2 cut(s) 21, 971
HpyF10VI GCNNNNNNNGC 8 cut(s) 209, 325, 413, 668, 818, 860, 1038, 1218
Hsp92II CATG 8 cut(s) 15, 383, 659, 704, 929, 1025, 1040, 1319
HspAI GCGC 1 cut(s) 1132
KroI GCCGGC 1 cut(s) 725
KroNI GCCGGC 1 cut(s) 727
Kzo9I GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
LmnI GCTCC 7 cut(s) 117, 123, 252, 499, 652, 691, 1156
Lsp1109I GCAGC 6 cut(s) 87, 199, 227, 428, 540, 1205
LweI GCATC 2 cut(s) 46, 405
MaeI CTAG 5 cut(s) 410, 860, 959, 1175, 1179
MaeIII GTNAC 3 cut(s) 836, 1085, 1183
MalI GATC 6 cut(s) 378, 555, 932, 975, 993, 1239
MbiI CCGCTC 1 cut(s) 120
MboI GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 16
MluNI TGGCCA 1 cut(s) 16
MlyI GAGTC 3 cut(s) 38, 494, 1052
MmeI TCCRAC 1 cut(s) 201
Mox20I TGGCCA 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 725
MscI TGGCCA 1 cut(s) 16
MseI TTAA 5 cut(s) 435, 645, 1095, 1214, 1326
MslI CAYNNNNRTG 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 16
MspA1I CMGCKG 2 cut(s) 212, 531
MspCI CTTAAG 1 cut(s) 1213
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 3 cut(s) 447, 690, 1343
Mva1269I GAATGC 3 cut(s) 255, 283, 1285
MvaI CCWGG 3 cut(s) 447, 690, 1343
MwoI GCNNNNNNNGC 8 cut(s) 209, 325, 413, 668, 818, 860, 1038, 1218
NaeI GCCGGC 1 cut(s) 727
NcoI CCATGG 1 cut(s) 1021
NdeII GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
NgoMIV GCCGGC 1 cut(s) 725
NheI GCTAGC 1 cut(s) 859
NlaIII CATG 8 cut(s) 15, 383, 659, 704, 929, 1025, 1040, 1319
NlaIV GGNNCC 3 cut(s) 501, 1158, 1207
NmeAIII GCCGAG 2 cut(s) 169, 1177
NmuCI GTSAC 2 cut(s) 836, 1085
NspI RCATGY 2 cut(s) 659, 704
NspV TTCGAA 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 70
PagI TCATGA 1 cut(s) 379
PcsI WCGNNNNNNNCGW 2 cut(s) 427, 595
PctI GAATGC 3 cut(s) 255, 283, 1285
PdiI GCCGGC 1 cut(s) 727
PfeI GAWTC 2 cut(s) 1063, 1171
PkrI GCNGC 7 cut(s) 77, 214, 217, 418, 530, 1220, 1223
PleI GAGTC 3 cut(s) 38, 493, 1051
PpsI GAGTC 3 cut(s) 38, 493, 1051
PpuMI RGGWCCY 1 cut(s) 1228
Psp5II RGGWCCY 1 cut(s) 1228
Psp6I CCWGG 3 cut(s) 445, 688, 1341
PspGI CCWGG 3 cut(s) 445, 688, 1341
PspN4I GGNNCC 3 cut(s) 501, 1158, 1207
PspPI GGNCC 5 cut(s) 406, 536, 706, 779, 1228
PspPPI RGGWCCY 1 cut(s) 1228
PspXI VCTCGAGB 1 cut(s) 70
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 531
PsuI RGATCY 1 cut(s) 553
PvuII CAGCTG 1 cut(s) 531
RsaI GTAC 3 cut(s) 463, 968, 1360
RsaNI GTAC 3 cut(s) 462, 967, 1359
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 5 cut(s) 435, 645, 1095, 1214, 1326
SatI GCNGC 7 cut(s) 76, 213, 216, 417, 529, 1219, 1222
Sau3AI GATC 6 cut(s) 376, 553, 930, 973, 991, 1237
Sau96I GGNCC 5 cut(s) 406, 536, 706, 779, 1228
SchI GAGTC 3 cut(s) 38, 494, 1052
ScrFI CCNGG 3 cut(s) 447, 690, 1343
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 2 cut(s) 46, 405
SfcI CTRYAG 2 cut(s) 213, 523
Sfr274I CTCGAG 1 cut(s) 70
SfuI TTCGAA 1 cut(s) 549
SinI GGWCC 3 cut(s) 536, 779, 1228
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 4 cut(s) 70, 475, 632, 1213
SmoI CTYRAG 4 cut(s) 70, 475, 632, 1213
SsiI CCGC 7 cut(s) 120, 141, 210, 760, 1032, 1210, 1221
SspMI CTAG 5 cut(s) 410, 860, 959, 1175, 1179
StyD4I CCNGG 3 cut(s) 445, 688, 1341
StyI CCWWGG 2 cut(s) 869, 1021
TaaI ACNGT 2 cut(s) 21, 971
TaqI TCGA 5 cut(s) 71, 333, 549, 933, 1105
TatI WGTACW 3 cut(s) 461, 966, 1358
TauI GCSGC 1 cut(s) 1224
TfiI GAWTC 2 cut(s) 1063, 1171
Tru1I TTAA 5 cut(s) 435, 645, 1095, 1214, 1326
Tru9I TTAA 5 cut(s) 435, 645, 1095, 1214, 1326
TscAI CASTG 2 cut(s) 307, 976
TseFI GTSAC 2 cut(s) 836, 1085
TseI GCWGC 6 cut(s) 75, 212, 215, 416, 528, 1218
Tsp45I GTSAC 2 cut(s) 836, 1085
TspDTI ATGAA 2 cut(s) 17, 914
TspGWI ACGGA 2 cut(s) 19, 37
TspRI CASTG 2 cut(s) 307, 976
Vha464I CTTAAG 1 cut(s) 1213
VpaK11BI GGWCC 3 cut(s) 536, 779, 1228
XapI RAATTY 2 cut(s) 467, 739
XceI RCATGY 2 cut(s) 659, 704
XhoI CTCGAG 1 cut(s) 70
XspI CTAG 5 cut(s) 410, 860, 959, 1175, 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.