MD10G1052200.v1.1

DNA polymerase alpha chain like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008292
Physical Location & Seq
Forward (+)
6918869 .. 6923827
4959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1052200.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGATGCCACAAACAACTTGAGCAGCCCAAAGAAAACAGCGAGGAAGAAGAAAAAGCGAGGTGGGAGCGGGAGCAAGAAGAAGATGACTGCCGAGCAGACTTTAGCTTTCAAGTCTGTGAGTGAATGGGTTTTCTTGGAACAGCACCAAGCTTCTTCCGCTGCAGCTTCGGATGATTTTGGGGTTTCGTTGGTGCAAAAGAGCATAGCAAACAAGCTACTCTTTGAATTGCATTCCCATTCAAATTGCAGTGATGGGTTTCTTTCGCCTTCCAAGTTGGTCGAGAGGGCTCATGAAAATGGGGTGAAAGTTCTTGCTCTGACAGATCATGACACAATGTCTGGCATCCCCGAGGCCCTAGAAGCAGCTCGGAGACATCGCATTAAGATAATTCCAGGTGTTGAAATAAGTACAAAATTCTACTCAAGAGGAGGGTCTGCACCAGAGGAGCCAGTTCACATCCTTGCATACTACAGCAGCTGTGGACCAACAAGTTTTGAAGATCTGGAAAAGTTCTTGTCAAATATAAGGGATGGTCGTTATCTTCGTGCAAAAAATATGGTCTTGAAATTGAACAAGCTTAAGTTCCCTCTTAAATGGGAGCATGTTGCAAAGATTGCAGGCAAGGGAGTTGCTCCTGGGAGAGTGCATGTGGCCCGTGCGATGGTTGAAGCCGGCTATGTGGAAAATTTGAAACAAGCTTTTGCCCGCTATCTATACGATGGTGGACCAGCATACTCTACGGGAAGCGAACCTCTGGCAGAAGAAGCAATAAAACTGATATGCGACACGGGAGGTGTGGCTGTGCTAGCTCATCCTTGGTCGTTGAAAAATCCAGTTGCCATCGTCAAAAGTTTGAAAGAAGCTGGTCTTCATGGGATCGAAGTTTACAGAAGTGATGGAAAACTAGCTGTGTACAGTGATCTTGCTGATACTTACGATCTGCTGAAGCTTGGAGGGTCAGATTACCATGGGAGAGGTGGGCATGGCGAGTCCGAGCTGGGTAGTGTGAATCTTCCAGTATTGGTTTTGCGTGACTTTCTTAAAGTAGCTCGACCAATTTGGTGCTGTTCCATTCGGCGCATTTTAGAAAATTATGCCGAGGAGCCTTCGGATTTGAATCTAGCAAGGGTTACGAGGTTTGAGAAAGGAGGTTCCCACTTAAGCTGCGGCAAGGACCTGATTGATCGTTGCTTCAATTTGTGGTTGACAAGTGAAGAGAGGCAGAATGCAGATTTTGAGGCCATTAGATTGAAGCTTTCCCATGTTTCAATTCATAAGGGAGGTATCCAGGTTCTTATAGAGAGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHP

Protein Analysis

438

Amino Acids

48.03

Weight (kDa)

9.0

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHP PF02811 76 - 146 4.7e-15 PHP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 69
AciI CCGC 4 cut(s) 69, 159, 709, 1170
AclWI GGATC 1 cut(s) 887
AcsI RAATTY 2 cut(s) 416, 688
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 3 cut(s) 412, 917, 1309
AfiI CCNNNNNNNGG 1 cut(s) 664
AflII CTTAAG 2 cut(s) 581, 1162
AhdI GACNNNNNGTC 1 cut(s) 337
AjnI CCWGG 3 cut(s) 394, 637, 1290
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 887
AlwNI CAGNNNCTG 1 cut(s) 480
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 3 cut(s) 354, 654, 1242
ApeKI GCWGC 6 cut(s) 24, 161, 164, 365, 477, 1167
ApoI RAATTY 2 cut(s) 416, 688
AspLEI GCGC 1 cut(s) 1083
AspS9I GGNCC 5 cut(s) 355, 485, 655, 728, 1177
AsuHPI GGTGA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 808
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 485, 728, 1177
BanII GRGCYC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 863
BbvI GCAGC 6 cut(s) 36, 148, 176, 377, 489, 1154
BccI CCATC 6 cut(s) 248, 527, 658, 716, 851, 893
BciT130I CCWGG 3 cut(s) 396, 639, 1292
BciVI GTATCC 1 cut(s) 1298
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 4 cut(s) 359, 809, 908, 1124
BfmI CTRYAG 2 cut(s) 162, 472
BfrI CTTAAG 2 cut(s) 581, 1162
BfuI GTATCC 1 cut(s) 1298
BglII AGATCT 1 cut(s) 502
BisI GCNGC 7 cut(s) 25, 162, 165, 366, 478, 1168, 1171
BlsI GCNGC 7 cut(s) 26, 163, 166, 367, 479, 1169, 1172
Bme1390I CCNGG 3 cut(s) 396, 639, 1292
Bme18I GGWCC 3 cut(s) 485, 728, 1177
BmeRI GACNNNNNGTC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 5 cut(s) 355, 485, 655, 728, 1177
BmiI GGNNCC 3 cut(s) 450, 1107, 1156
BmrFI CCNGG 3 cut(s) 396, 639, 1292
BmsI GCATC 1 cut(s) 354
BmtI GCTAGC 1 cut(s) 812
BpiI GAAGAC 1 cut(s) 863
BpuEI CTTGAG 2 cut(s) 40, 409
BsaBI GATNNNNATC 1 cut(s) 1119
BsaJI CCNNGG 5 cut(s) 351, 638, 818, 970, 1101
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 3 cut(s) 452, 836, 1019
Bse8I GATNNNNATC 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 396, 639, 1292
BseDI CCNNGG 5 cut(s) 351, 638, 818, 970, 1101
BseGI GGATG 6 cut(s) 10, 178, 345, 459, 538, 814
BseJI GATNNNNATC 1 cut(s) 1119
BseLI CCNNNNNNNGG 1 cut(s) 664
BseNI ACTGG 3 cut(s) 452, 836, 1019
BseRI GAGGAG 3 cut(s) 444, 461, 1118
BseXI GCAGC 6 cut(s) 36, 148, 176, 377, 489, 1154
BseYI CCCAGC 1 cut(s) 1000
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 3 cut(s) 356, 656, 1244
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 675
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 1 cut(s) 367
BsmI GAATGC 2 cut(s) 232, 1234
BsnI GGCC 3 cut(s) 356, 656, 1244
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
Bsp19I CCATGG 1 cut(s) 970
BspACI CCGC 4 cut(s) 69, 159, 709, 1170
BspANI GGCC 3 cut(s) 356, 656, 1244
BspHI TCATGA 2 cut(s) 292, 328
BspLI GGNNCC 3 cut(s) 450, 1107, 1156
BspMAI CTGCAG 1 cut(s) 166
BspOI GCTAGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 887
BspTI CTTAAG 2 cut(s) 581, 1162
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 674
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 3 cut(s) 452, 836, 1019
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 5 cut(s) 351, 638, 818, 970, 1101
BssMI GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
BssT1I CCWWGG 2 cut(s) 818, 970
Bst2UI CCWGG 3 cut(s) 396, 639, 1292
Bst4CI ACNGT 1 cut(s) 920
Bst6I CTCTTC 1 cut(s) 1212
BstAFI CTTAAG 2 cut(s) 581, 1162
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 915
BstC8I GCNNGC 4 cut(s) 622, 676, 709, 810
BstDSI CCRYGG 1 cut(s) 970
BstF5I GGATG 6 cut(s) 10, 178, 345, 459, 538, 814
BstHHI GCGC 1 cut(s) 1083
BstKTI GATC 6 cut(s) 328, 505, 882, 925, 943, 1189
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
BstMWI GCNNNNNNNGC 5 cut(s) 158, 362, 617, 767, 809
BstNI CCWGG 3 cut(s) 396, 639, 1292
BstNSI RCATGY 2 cut(s) 608, 653
BstSCI CCNGG 3 cut(s) 394, 637, 1290
BstSFI CTRYAG 2 cut(s) 162, 472
BstV1I GCAGC 6 cut(s) 36, 148, 176, 377, 489, 1154
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuI GTATCC 1 cut(s) 1298
BsuRI GGCC 3 cut(s) 356, 656, 1244
BtgI CCRYGG 1 cut(s) 970
BtgZI GCGATG 2 cut(s) 362, 677
BtsCI GGATG 6 cut(s) 10, 178, 345, 459, 538, 814
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 256, 925
Cac8I GCNNGC 4 cut(s) 622, 676, 709, 810
CaiI CAGNNNCTG 1 cut(s) 480
CciI TCATGA 2 cut(s) 292, 328
CfoI GCGC 1 cut(s) 1083
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 5 cut(s) 355, 485, 655, 728, 1177
Csp6I GTAC 3 cut(s) 411, 916, 1308
CviAII CATG 8 cut(s) 293, 329, 605, 650, 875, 971, 986, 1265
CviQI GTAC 3 cut(s) 411, 916, 1308
DpnI GATC 6 cut(s) 327, 504, 881, 924, 942, 1188
DpnII GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
DriI GACNNNNNGTC 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 1212
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 1212
Eco130I CCWWGG 2 cut(s) 818, 970
Eco24I GRGCYC 1 cut(s) 292
Eco47I GGWCC 3 cut(s) 485, 728, 1177
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 2 cut(s) 355, 1177
EcoRII CCWGG 3 cut(s) 394, 637, 1290
EcoT14I CCWWGG 2 cut(s) 818, 970
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 2 cut(s) 818, 970
FaeI CATG 8 cut(s) 296, 332, 608, 653, 878, 974, 989, 1268
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 855, 887
FatI CATG 8 cut(s) 292, 328, 604, 649, 874, 970, 985, 1264
FauI CCCGC 2 cut(s) 62, 716
Fnu4HI GCNGC 7 cut(s) 25, 162, 165, 366, 478, 1168, 1171
FokI GGATG 6 cut(s) 17, 185, 332, 446, 545, 801
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 7 cut(s) 25, 162, 165, 366, 478, 1168, 1171
FspBI CTAG 4 cut(s) 359, 809, 908, 1124
GlaI GCGC 1 cut(s) 1082
GluI GCNGC 7 cut(s) 25, 162, 165, 366, 478, 1168, 1171
GsaI CCCAGC 1 cut(s) 1004
HaeIII GGCC 3 cut(s) 356, 656, 1244
HapII CCGG 1 cut(s) 675
HhaI GCGC 1 cut(s) 1083
Hin1II CATG 8 cut(s) 296, 332, 608, 653, 878, 974, 989, 1268
Hin6I GCGC 1 cut(s) 1081
HinP1I GCGC 1 cut(s) 1081
HincII GTYRAC 1 cut(s) 1209
HindII GTYRAC 1 cut(s) 1209
HindIII AAGCTT 5 cut(s) 150, 578, 699, 950, 1256
HinfI GANTC 3 cut(s) 992, 1012, 1120
HpaII CCGG 1 cut(s) 675
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 6 cut(s) 457, 485, 728, 889, 916, 1209
Hpy188I TCNGA 6 cut(s) 172, 321, 372, 964, 997, 1114
Hpy188III TCNNGA 6 cut(s) 283, 293, 329, 426, 506, 565
Hpy8I GTNNAC 6 cut(s) 457, 485, 728, 889, 916, 1209
HpyAV CCTTC 2 cut(s) 279, 1119
HpyCH4III ACNGT 1 cut(s) 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 362, 617, 767, 809
Hsp92II CATG 8 cut(s) 296, 332, 608, 653, 878, 974, 989, 1268
HspAI GCGC 1 cut(s) 1081
KroI GCCGGC 1 cut(s) 674
KroNI GCCGGC 1 cut(s) 676
Kzo9I GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
LmnI GCTCC 6 cut(s) 66, 72, 448, 601, 640, 1105
Lsp1109I GCAGC 6 cut(s) 36, 148, 176, 377, 489, 1154
LweI GCATC 1 cut(s) 354
MaeI CTAG 4 cut(s) 359, 809, 908, 1124
MaeIII GTNAC 2 cut(s) 1034, 1132
MalI GATC 6 cut(s) 327, 504, 881, 924, 942, 1188
MbiI CCGCTC 1 cut(s) 69
MboI GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 292
MlyI GAGTC 1 cut(s) 1001
MroNI GCCGGC 1 cut(s) 674
MseI TTAA 5 cut(s) 384, 582, 594, 1044, 1163
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 161, 480
MspCI CTTAAG 2 cut(s) 581, 1162
MspI CCGG 1 cut(s) 675
MspR9I CCNGG 3 cut(s) 396, 639, 1292
Mva1269I GAATGC 2 cut(s) 232, 1234
MvaI CCWGG 3 cut(s) 396, 639, 1292
MwoI GCNNNNNNNGC 5 cut(s) 158, 362, 617, 767, 809
NaeI GCCGGC 1 cut(s) 676
NcoI CCATGG 1 cut(s) 970
NdeII GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
NgoMIV GCCGGC 1 cut(s) 674
NheI GCTAGC 1 cut(s) 808
NlaIII CATG 8 cut(s) 296, 332, 608, 653, 878, 974, 989, 1268
NlaIV GGNNCC 3 cut(s) 450, 1107, 1156
NmeAIII GCCGAG 2 cut(s) 118, 1126
NmuCI GTSAC 1 cut(s) 1034
NspI RCATGY 2 cut(s) 608, 653
PagI TCATGA 2 cut(s) 292, 328
PcsI WCGNNNNNNNCGW 1 cut(s) 544
PctI GAATGC 2 cut(s) 232, 1234
PdiI GCCGGC 1 cut(s) 676
PfeI GAWTC 2 cut(s) 1012, 1120
PkrI GCNGC 7 cut(s) 26, 163, 166, 367, 479, 1169, 1172
PleI GAGTC 1 cut(s) 1000
PpsI GAGTC 1 cut(s) 1000
PpuMI RGGWCCY 1 cut(s) 1177
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 3 cut(s) 394, 637, 1290
PspFI CCCAGC 1 cut(s) 1000
PspGI CCWGG 3 cut(s) 394, 637, 1290
PspN4I GGNNCC 3 cut(s) 450, 1107, 1156
PspPI GGNCC 5 cut(s) 355, 485, 655, 728, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 480
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 480
RsaI GTAC 3 cut(s) 412, 917, 1309
RsaNI GTAC 3 cut(s) 411, 916, 1308
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 5 cut(s) 384, 582, 594, 1044, 1163
SatI GCNGC 7 cut(s) 25, 162, 165, 366, 478, 1168, 1171
Sau3AI GATC 6 cut(s) 325, 502, 879, 922, 940, 1186
Sau96I GGNCC 5 cut(s) 355, 485, 655, 728, 1177
SchI GAGTC 1 cut(s) 1001
ScrFI CCNGG 3 cut(s) 396, 639, 1292
SduI GDGCHC 1 cut(s) 292
SfaNI GCATC 1 cut(s) 354
SfcI CTRYAG 2 cut(s) 162, 472
SinI GGWCC 3 cut(s) 485, 728, 1177
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 4 cut(s) 19, 424, 581, 1162
SmoI CTYRAG 4 cut(s) 19, 424, 581, 1162
SsiI CCGC 4 cut(s) 69, 159, 709, 1170
SspMI CTAG 4 cut(s) 359, 809, 908, 1124
StyD4I CCNGG 3 cut(s) 394, 637, 1290
StyI CCWWGG 2 cut(s) 818, 970
TaaI ACNGT 1 cut(s) 920
TaqI TCGA 3 cut(s) 282, 882, 1054
TatI WGTACW 3 cut(s) 410, 915, 1307
TauI GCSGC 1 cut(s) 1173
TfiI GAWTC 2 cut(s) 1012, 1120
Tru1I TTAA 5 cut(s) 384, 582, 594, 1044, 1163
Tru9I TTAA 5 cut(s) 384, 582, 594, 1044, 1163
TscAI CASTG 2 cut(s) 256, 925
TseFI GTSAC 1 cut(s) 1034
TseI GCWGC 6 cut(s) 24, 161, 164, 365, 477, 1167
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 3 cut(s) 309, 863, 1265
TspRI CASTG 2 cut(s) 256, 925
Vha464I CTTAAG 2 cut(s) 581, 1162
VpaK11BI GGWCC 3 cut(s) 485, 728, 1177
XapI RAATTY 2 cut(s) 416, 688
XceI RCATGY 2 cut(s) 608, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XspI CTAG 4 cut(s) 359, 809, 908, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.