FvH4_1g25685

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016247
Physical Location & Seq
Forward (+)
17582060 .. 17584947
2888 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25685.t1

Sequence Viewer

Length: 1515 bp
ATGGCGGATGAAAGACATTTGAAAAGGAATGAAAGATCTGGAAATGTGCCGGCAATACCTTGTTTGGATGAAGAACTTCATGCCATTGTTGATACTTTGTTTACTGACGGGGTGATCAAGCCTGTGCAGCAAGAAAAGCCCCCAAACAGGGAAGATAAGAAGAACCCAAGGTACTGTCGTTATCACCAAATAGTGGAACATCCTACTCCTGCATGTCAAACCTTGAGAAAGATCCTCCATGCAAAAATCGACAAGGGCACGCTCGAGCTTCCATCAAAGAAGCAAGCCACATATGATGATCCATTGCCGAAACGTCATGGAAAGGGTGTTTGTACTGTCATCACTGGTGTTGATAAAACAGGCCAAGGTAAAGAGAATATGTATGGAGCTTTAGCACTGAGTGTGTATCAAAGTCCCATCGATCAAGATGCTTGGTATCAACATTGCAGGGGTCAAGGTTACGCCGACCCTTTCTTCACCCAATATAATTGGAATAACAACTCAACCATGGAAGGCCAAAAGCGAGCATGCTTCAGCAACGATGATCATTGTTACCAGGACTATCAAGTCACTAAAGTAGATGATGACATTGTGGAGGCCAATTTCATGCGGAGCAACATGCCTTCTGCCGTGGAAGTCCTTCAACAAAATCCTAAGTTCAAGTCCCTCTTTAATAAGCTAGAATACGAACCTGAGTCGAGGTATGCTACTACAAAGGCCCTGATGGATGTATTTGAGACATTTGGTCCCCAATGTTTTGCTACAGGAACTGAAACACAAAGGGGCAGAAGTTTATTTGAAGGTGACAATGTTATTGTATTCACTGATAAAGACATGGAGGTAGATTATCCTGATCATCGGAGGCCACTATACTTAGAAGCTCAAATCAATGATGTACTTGTGAGGCGAGCACTGGTTGACACAGGATCATCCTTGAATATCATATCTCTCCAAGTGTTAACTGTTGCTGCTATTAAGGGGCAAGTTGTGATTGATATGATCACTTTGTTCTTAGGGGAAGAAGGCTTTGATGTCTCACAAGAAATATTAGGAGAGTTACCTGAGATCTCGGCAGTAGTTGAAGAAGAAGAGCCCTGGACACTCCATTTTGATGGGTTAGCCACATCTAATGGCAGAGGAGCAGGAGTTGTGCTTGTCGATCCTAAAGGGCATGCCAAGCCTTTCTCTTTCAAGCTCGATTTTCCATGCACTAATAATGTGGCAAAGTACGAAGCTTTTATTGTTGGGCTCTCAACTGCACGAGAGATGAATGTGAAGAAAATTAGAGCAATCGGAGATTCAAACCTGGTCATTAGTCAATTGAAAGGAGATTTTGTAGTGAAAGAACCAGCTTTGGCGCCTTATAGAACTTTGGCAGAGAAGCTAGTCACAAGCTTTGAGCAAATAAGTCTAGAGCACATTCTTGGCAACACCAACAGATACGCAGACACTCTAGCTACCCTTGGTTCAAGACTTTCATTCACAAATGAGCAACCCAACATCACTGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

505

Amino Acids

56.62

Weight (kDa)

5.37

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 370 - 487 4.2e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 1357
AccB7I CCANNNNNTGG 1 cut(s) 193
AciI CCGC 2 cut(s) 5, 610
AclWI GGATC 4 cut(s) 226, 293, 934, 1154
AcuI CTGAAG 1 cut(s) 517
AcyI GRCGYC 1 cut(s) 1358
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 4 cut(s) 173, 334, 897, 1229
AfiI CCNNNNNNNGG 3 cut(s) 147, 148, 193
AhdI GACNNNNNGTC 1 cut(s) 744
AjnI CCWGG 3 cut(s) 555, 1094, 1305
Alw21I GWGCWC 2 cut(s) 913, 1419
Alw26I GTCTC 2 cut(s) 731, 1039
AlwI GGATC 4 cut(s) 226, 293, 934, 1154
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 5 cut(s) 361, 514, 597, 717, 863
ApeKI GCWGC 2 cut(s) 127, 968
Asp700I GAANNNNTTC 2 cut(s) 75, 639
AspLEI GCGC 1 cut(s) 1360
AspS9I GGNCC 2 cut(s) 718, 746
AsuHPI GGTGA 4 cut(s) 124, 176, 469, 815
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 260
BaeI ACNNNNGTAYC 2 cut(s) 163, 196
BanI GGYRCC 1 cut(s) 1357
BanII GRGCYC 2 cut(s) 1095, 1251
BauI CACGAG 1 cut(s) 1260
Bbv12I GWGCWC 2 cut(s) 913, 1419
BbvI GCAGC 2 cut(s) 139, 955
BccI CCATC 4 cut(s) 280, 425, 718, 1106
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 3 cut(s) 557, 1096, 1307
BclI TGATCA 4 cut(s) 114, 544, 853, 999
BcoDI GTCTC 2 cut(s) 731, 1039
BfaI CTAG 4 cut(s) 680, 1385, 1412, 1454
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 1361
BglII AGATCT 2 cut(s) 35, 1065
BisI GCNGC 2 cut(s) 128, 969
BlsI GCNGC 2 cut(s) 129, 970
Bme1390I CCNGG 3 cut(s) 557, 1096, 1307
Bme18I GGWCC 1 cut(s) 746
BmeRI GACNNNNNGTC 1 cut(s) 744
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 718, 746
BmiI GGNNCC 2 cut(s) 748, 1359
BmrFI CCNGG 3 cut(s) 557, 1096, 1307
BmsI GCATC 1 cut(s) 418
BpuEI CTTGAG 1 cut(s) 244
Bsa29I ATCGAT 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 1358
BsaJI CCNNGG 6 cut(s) 167, 364, 507, 630, 1094, 1462
BsaXI ACNNNNNCTCC 4 cut(s) 1131, 1161, 1320, 1350
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 148, 193
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 349, 918
Bse3DI GCAATG 2 cut(s) 302, 442
BseBI CCWGG 3 cut(s) 557, 1096, 1307
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 6 cut(s) 167, 364, 507, 630, 1094, 1462
BseGI GGATG 5 cut(s) 13, 73, 199, 733, 929
BseLI CCNNNNNNNGG 3 cut(s) 147, 148, 193
BseMI GCAATG 2 cut(s) 302, 442
BseMII CTCAG 3 cut(s) 389, 684, 1053
BseNI ACTGG 2 cut(s) 349, 918
BseRI GAGGAG 1 cut(s) 1152
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 2 cut(s) 139, 955
BsgI GTGCAG 2 cut(s) 146, 1242
BshFI GGCC 5 cut(s) 363, 516, 599, 719, 865
BshNI GGYRCC 1 cut(s) 1357
BshVI ATCGAT 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 913, 1419
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 3 cut(s) 399, 649, 732
BslI CCNNNNNNNGG 3 cut(s) 147, 148, 193
BsmAI GTCTC 2 cut(s) 731, 1039
BsmFI GGGAC 3 cut(s) 399, 649, 732
BsnI GGCC 5 cut(s) 363, 516, 599, 719, 865
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 5 cut(s) 260, 913, 1095, 1251, 1419
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 2 cut(s) 5, 610
BspANI GGCC 5 cut(s) 363, 516, 599, 719, 865
BspCNI CTCAG 3 cut(s) 390, 685, 1054
BspDI ATCGAT 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 748, 1359
BspPI GGATC 4 cut(s) 226, 293, 934, 1154
BspQI GCTCTTC 1 cut(s) 1083
BspT107I GGYRCC 1 cut(s) 1357
BsrDI GCAATG 2 cut(s) 302, 442
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 2 cut(s) 349, 918
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 6 cut(s) 167, 364, 507, 630, 1094, 1462
BssNI GRCGYC 1 cut(s) 1358
BssSI CACGAG 1 cut(s) 1260
BssT1I CCWWGG 4 cut(s) 167, 364, 507, 1462
Bst2BI CACGAG 1 cut(s) 1260
Bst2UI CCWGG 3 cut(s) 557, 1096, 1307
Bst4CI ACNGT 4 cut(s) 176, 337, 964, 1507
Bst6I CTCTTC 1 cut(s) 1083
BstACI GRCGYC 1 cut(s) 1358
BstC8I GCNNGC 7 cut(s) 51, 260, 285, 525, 529, 909, 1173
BstDEI CTNAG 6 cut(s) 398, 654, 693, 874, 1012, 1062
BstDSI CCRYGG 2 cut(s) 507, 630
BstF5I GGATG 5 cut(s) 13, 73, 199, 733, 929
BstH2I RGCGCY 1 cut(s) 1361
BstHHI GCGC 1 cut(s) 1360
BstMAI GTCTC 2 cut(s) 731, 1039
BstMWI GCNNNNNNNGC 3 cut(s) 127, 136, 1177
BstNI CCWGG 3 cut(s) 557, 1096, 1307
BstNSI RCATGY 4 cut(s) 216, 531, 622, 1175
BstSCI CCNGG 3 cut(s) 555, 1094, 1305
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 139, 955
BstX2I RGATCY 3 cut(s) 35, 231, 1065
BstXI CCANNNNNNTGG 1 cut(s) 1112
BstYI RGATCY 3 cut(s) 35, 231, 1065
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 5 cut(s) 363, 516, 599, 719, 865
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 2 cut(s) 507, 630
BtsCI GGATG 5 cut(s) 13, 73, 199, 733, 929
BtsIMutI CAGTG 5 cut(s) 342, 395, 822, 911, 1503
Cac8I GCNNGC 7 cut(s) 51, 260, 285, 525, 529, 909, 1173
CaiI CAGNNNCTG 1 cut(s) 770
CfoI GCGC 1 cut(s) 1360
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 2 cut(s) 718, 746
ClaI ATCGAT 1 cut(s) 420
CsiI ACCWGGT 1 cut(s) 1305
Csp6I GTAC 4 cut(s) 172, 333, 896, 1228
CviQI GTAC 4 cut(s) 172, 333, 896, 1228
DdeI CTNAG 6 cut(s) 398, 654, 693, 874, 1012, 1062
DinI GGCGCC 1 cut(s) 1359
DraIII CACNNNGTG 1 cut(s) 401
DrdI GACNNNNNNGTC 1 cut(s) 566
DriI GACNNNNNGTC 1 cut(s) 744
DseDI GACNNNNNNGTC 1 cut(s) 566
Eam1104I CTCTTC 1 cut(s) 1083
Eam1105I GACNNNNNGTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 1083
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 167, 364, 507, 1462
Eco24I GRGCYC 2 cut(s) 1095, 1251
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 517
Eco88I CYCGRG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 718
EcoRII CCWGG 3 cut(s) 555, 1094, 1305
EcoT14I CCWWGG 4 cut(s) 167, 364, 507, 1462
EcoT38I GRGCYC 2 cut(s) 1095, 1251
EgeI GGCGCC 1 cut(s) 1359
EheI GGCGCC 1 cut(s) 1359
ErhI CCWWGG 4 cut(s) 167, 364, 507, 1462
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FaqI GGGAC 3 cut(s) 399, 649, 732
FauNDI CATATG 1 cut(s) 292
FbaI TGATCA 4 cut(s) 114, 544, 853, 999
Fnu4HI GCNGC 2 cut(s) 128, 969
FokI GGATG 5 cut(s) 20, 80, 186, 740, 916
FriOI GRGCYC 2 cut(s) 1095, 1251
Fsp4HI GCNGC 2 cut(s) 128, 969
FspBI CTAG 4 cut(s) 680, 1385, 1412, 1454
GlaI GCGC 1 cut(s) 1359
GluI GCNGC 2 cut(s) 128, 969
HaeII RGCGCY 1 cut(s) 1361
HaeIII GGCC 5 cut(s) 363, 516, 599, 719, 865
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 1360
Hin1I GRCGYC 1 cut(s) 1358
Hin6I GCGC 1 cut(s) 1358
HinP1I GCGC 1 cut(s) 1358
HincII GTYRAC 2 cut(s) 919, 960
HindII GTYRAC 2 cut(s) 919, 960
HindIII AAGCTT 2 cut(s) 1233, 1393
HinfI GANTC 2 cut(s) 695, 1298
HpaI GTTAAC 1 cut(s) 960
HpaII CCGG 1 cut(s) 50
HphI GGTGA 4 cut(s) 124, 176, 469, 815
Hpy166II GTNNAC 3 cut(s) 102, 919, 960
Hpy188I TCNGA 2 cut(s) 861, 1295
Hpy188III TCNNGA 5 cut(s) 39, 425, 851, 1412, 1470
Hpy8I GTNNAC 3 cut(s) 102, 919, 960
HpyAV CCTTC 5 cut(s) 506, 633, 650, 794, 1016
HpyCH4III ACNGT 4 cut(s) 176, 337, 964, 1507
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 6 cut(s) 127, 212, 242, 447, 1209, 1259
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 136, 1177
HpyF3I CTNAG 6 cut(s) 398, 654, 693, 874, 1012, 1062
HpySE526I ACGT 1 cut(s) 313
Hsp92I GRCGYC 1 cut(s) 1358
HspAI GCGC 1 cut(s) 1358
KasI GGCGCC 1 cut(s) 1357
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Ksp22I TGATCA 4 cut(s) 114, 544, 853, 999
KspAI GTTAAC 1 cut(s) 960
LguI GCTCTTC 1 cut(s) 1083
LmnI GCTCC 3 cut(s) 386, 612, 1139
Lsp1109I GCAGC 2 cut(s) 139, 955
LweI GCATC 1 cut(s) 418
MabI ACCWGGT 1 cut(s) 1305
MaeI CTAG 4 cut(s) 680, 1385, 1412, 1454
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 6 cut(s) 458, 551, 568, 803, 1056, 1387
MboII GAAGA 9 cut(s) 83, 164, 172, 466, 1031, 1094, 1097, 1100, 1288
MfeI CAATTG 1 cut(s) 1319
MflI RGATCY 3 cut(s) 35, 231, 1065
MhlI GDGCHC 5 cut(s) 260, 913, 1095, 1251, 1419
MluCI AATT 4 cut(s) 487, 601, 1281, 1319
Mly113I GGCGCC 1 cut(s) 1358
MlyI GAGTC 1 cut(s) 704
MnlI CCTC 8 cut(s) 245, 589, 677, 693, 832, 855, 897, 1130
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 2 cut(s) 75, 639
MseI TTAA 3 cut(s) 672, 959, 975
MslI CAYNNNNRTG 2 cut(s) 1110, 1508
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 3 cut(s) 557, 1096, 1307
MunI CAATTG 1 cut(s) 1319
MvaI CCWGG 3 cut(s) 557, 1096, 1307
MwoI GCNNNNNNNGC 3 cut(s) 127, 136, 1177
NaeI GCCGGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 1358
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 292
NgoMIV GCCGGC 1 cut(s) 49
NlaIV GGNNCC 2 cut(s) 748, 1359
NmeAIII GCCGAG 1 cut(s) 1049
NmuCI GTSAC 3 cut(s) 568, 803, 1387
NspI RCATGY 4 cut(s) 216, 531, 622, 1175
PaeI GCATGC 2 cut(s) 531, 1175
PaeR7I CTCGAG 1 cut(s) 263
PciSI GCTCTTC 1 cut(s) 1083
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 2 cut(s) 75, 639
PfeI GAWTC 1 cut(s) 1298
PflMI CCANNNNNTGG 1 cut(s) 193
PkrI GCNGC 2 cut(s) 129, 970
PleI GAGTC 1 cut(s) 703
PluTI GGCGCC 1 cut(s) 1361
PpsI GAGTC 1 cut(s) 703
Psp6I CCWGG 3 cut(s) 555, 1094, 1305
PspGI CCWGG 3 cut(s) 555, 1094, 1305
PspN4I GGNNCC 2 cut(s) 748, 1359
PspPI GGNCC 2 cut(s) 718, 746
PspXI VCTCGAGB 1 cut(s) 263
PsrI GAACNNNNNNTAC 2 cut(s) 155, 187
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 3 cut(s) 35, 231, 1065
RsaI GTAC 4 cut(s) 173, 334, 897, 1229
RsaNI GTAC 4 cut(s) 172, 333, 896, 1228
RseI CAYNNNNRTG 2 cut(s) 1110, 1508
SapI GCTCTTC 1 cut(s) 1083
SaqAI TTAA 3 cut(s) 672, 959, 975
SatI GCNGC 2 cut(s) 128, 969
Sau96I GGNCC 2 cut(s) 718, 746
SchI GAGTC 1 cut(s) 704
ScrFI CCNGG 3 cut(s) 557, 1096, 1307
SduI GDGCHC 5 cut(s) 260, 913, 1095, 1251, 1419
SexAI ACCWGGT 1 cut(s) 1305
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 762
SfoI GGCGCC 1 cut(s) 1359
Sfr274I CTCGAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 746
SlaI CTCGAG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 1110, 1508
SmlI CTYRAG 2 cut(s) 223, 263
SmoI CTYRAG 2 cut(s) 223, 263
SphI GCATGC 2 cut(s) 531, 1175
Sse9I AATT 4 cut(s) 487, 601, 1281, 1319
SsiI CCGC 2 cut(s) 5, 610
SspDI GGCGCC 1 cut(s) 1357
SspI AATATT 1 cut(s) 1047
SspMI CTAG 4 cut(s) 680, 1385, 1412, 1454
StyD4I CCNGG 3 cut(s) 555, 1094, 1305
StyI CCWWGG 4 cut(s) 167, 364, 507, 1462
TaaI ACNGT 4 cut(s) 176, 337, 964, 1507
TaiI ACGT 1 cut(s) 316
TaqI TCGA 6 cut(s) 249, 264, 420, 698, 1158, 1197
TasI AATT 4 cut(s) 487, 601, 1281, 1319
TatI WGTACW 2 cut(s) 332, 895
TfiI GAWTC 1 cut(s) 1298
Tru1I TTAA 3 cut(s) 672, 959, 975
Tru9I TTAA 3 cut(s) 672, 959, 975
TscAI CASTG 5 cut(s) 349, 402, 829, 918, 1510
TseFI GTSAC 3 cut(s) 568, 803, 1387
TseI GCWGC 2 cut(s) 127, 968
Tsp45I GTSAC 3 cut(s) 568, 803, 1387
TspDTI ATGAA 7 cut(s) 24, 45, 68, 84, 595, 1283, 1467
TspRI CASTG 5 cut(s) 349, 402, 829, 918, 1510
Van91I CCANNNNNTGG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 746
XbaI TCTAGA 1 cut(s) 1411
XceI RCATGY 4 cut(s) 216, 531, 622, 1175
XhoI CTCGAG 1 cut(s) 263
XmnI GAANNNNTTC 2 cut(s) 75, 639
XspI CTAG 4 cut(s) 680, 1385, 1412, 1454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.