FvH4_1g29804

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016247
Physical Location & Seq
Forward (+)
23364956 .. 23367024
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29804.t1

Sequence Viewer

Length: 1569 bp
ATGGCTCCTTCACGCAACAAGAGAAGCAAGATGCCTTCACAAGATGGGGGAGAGCGTCAAGAAGATGAAGAGCGTCAGGAGGAGTTTGCCTCTAGGCAACATGAAGAGTTGCCGCGCACCAATGAAGATTGGAAGTACCTCCCCCAACCACCTTACTTAGCAAGCTTGGTCAATCGCCCTTATCCCAAAGGTTATGAAACACCAAGCTTTGTCCTCTTTGATGGGAGAAAAGGAAGTCCAAGAGAGCACATAAGCCGTTTCATTGATGCTCTTGGACCTCACGCTGGTGATCATAATCTCAGGCTCCGTGAGTTCTCAAAAACCCTCACCGACCGTGCCTACACTTGGTATACTACTCTAGCACCGGGTTCTATTCGTACTTGGGAAGATATGGCAGACAAGTTTTGCAAAAAATACTTCGAGCATGAGGAGAGAGCCACTTTGGCTCAACTCAACAGCACTCGCCAAAGATCAAGCGAAAATCTAGTTGAATTCGTGAGGCGCTACAGAGACCTTGCTTTGGATTGCTATGATGAGAAAGGAGAAGAAGCCTTGGTGGAGATCTGCGTCGGTAACATGCTACCTGAATACAGGGTGTACCTTGAGAATGCAAACATAGGTACCTTTACACGCTTGATGGACGCTGTAAAGAAGACAAGCATGTCTGTCAAGGTTGTTAATGAAAATAGAACATGGAGGTCAGAGAAGGATACACATCAAGCATTGACAATTGGTGAAAGGCACTCAAACTCAAATAAGCGCAAGGATAGAGAAACTTATCCTGCTTTGCCATGCTGCGATGAAGAACTTAATGCCATCCTAGATACCATGTACCACCGGCTTGTCGGTCATCCTTCATCTGCTTGCCAATTCCTAAGGAAGTTTCTTAATGAAAAGATCCAGGATGAGACTTTAGCTATACCCTCCAAGAAGCAAGCTATCGATGATGATCATTTGCCTCGCCGACGGCCAGGAAAAGAAACAGCCGTTGTCATAACTTGCTTTGAAGATGATATGGAGGAAGGAAGCCGCCCTAGTGGCCAGGTGCCCTACTTGGTTACGGAGCCGAAAGAAGGATGCTTTGACATAGTTTCTTGGAATAAGAGTTGTATGCCACAGAGTTACAATACCCCATGCTCTGATCCCATAGAGTGGAAGGAAGATGAGCATGCTCACTATCACATGGGGGATTCAAGCAACAAAGAAGCGACTGGAGTGACATTCACTGATTTGGAGGAGAATGTGGCAAGGTCTCACCTCAACGAAAGCAAAAAGCAGCGATCTTATCCAACATGTTCACGAGTCCTTCAACAGAGCCCAAAGTTCAAATCTCTTTTTGATCAGCTTGAGTACGGACAACAGGCTAGGCAAGCAGCAGTAGAAGCACTCATGCAGGTCTCAGCTGATTTTGGGCCTCAAAGATCTTCAATTGAGTCAGCCAACAATAGGGCATTCATGGAGGACCCTAATAGTGAAGCTTATCAAAGCAGATACTACATGCTTGTCTCACAAGATGTTGTGACAATGGATCCCATCAACAGCAAATGGCTCAAGCTTTATCATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag

Protein Analysis

523

Amino Acids

60.4

Weight (kDa)

5.78

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 105 - 189 7.8e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 661
Acc36I ACCTGC 1 cut(s) 1384
Acc65I GGTACC 1 cut(s) 620
AccB1I GGYRCC 2 cut(s) 620, 1045
AccB7I CCANNNNNTGG 1 cut(s) 1152
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 115
AciI CCGC 2 cut(s) 113, 1030
AclWI GGATC 4 cut(s) 892, 1136, 1523, 1536
AcoI YGGCCR 2 cut(s) 968, 1039
AcsI RAATTY 1 cut(s) 491
AfaI GTAC 6 cut(s) 137, 379, 599, 622, 833, 1352
AfiI CCNNNNNNNGG 6 cut(s) 284, 345, 520, 1073, 1152, 1446
AflIII ACRYGT 1 cut(s) 1292
AgsI TTSAA 6 cut(s) 491, 1007, 1194, 1310, 1327, 1428
AjnI CCWGG 3 cut(s) 900, 970, 1041
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 8 cut(s) 165, 207, 917, 938, 1345, 1403, 1478, 1555
AluI AGCT 8 cut(s) 165, 207, 917, 938, 1345, 1403, 1478, 1555
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 5 cut(s) 504, 902, 1257, 1402, 1510
AlwI GGATC 4 cut(s) 892, 1136, 1523, 1536
AoxI GGCC 3 cut(s) 968, 1039, 1412
ApeKI GCWGC 3 cut(s) 795, 1276, 1373
ApoI RAATTY 1 cut(s) 491
Asp718I GGTACC 1 cut(s) 620
AspLEI GCGC 3 cut(s) 117, 504, 762
AspS9I GGNCC 3 cut(s) 275, 1412, 1462
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 4 cut(s) 299, 319, 746, 1247
AvaII GGWCC 2 cut(s) 275, 1462
AxyI CCTNAGG 1 cut(s) 875
BaeGI GKGCMC 1 cut(s) 1050
BaeI ACNNNNGTAYC 2 cut(s) 604, 637
BalI TGGCCA 1 cut(s) 1041
BamHI GGATCC 1 cut(s) 1528
BanI GGYRCC 2 cut(s) 620, 1045
BanII GRGCYC 1 cut(s) 1319
BauI CACGAG 1 cut(s) 1299
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 3 cut(s) 782, 1288, 1385
BccI CCATC 5 cut(s) 38, 215, 631, 824, 1541
BceAI ACGGC 3 cut(s) 240, 971, 983
BciT130I CCWGG 3 cut(s) 902, 972, 1043
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 3 cut(s) 289, 949, 1339
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 5 cut(s) 504, 902, 1257, 1402, 1510
BfaI CTAG 6 cut(s) 93, 359, 485, 821, 1035, 1365
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 1384
BfuI GTATCC 1 cut(s) 703
BglI GCCNNNNNGGC 2 cut(s) 443, 1038
BglII AGATCT 2 cut(s) 561, 1421
BisI GCNGC 5 cut(s) 113, 796, 1030, 1277, 1374
BlsI GCNGC 5 cut(s) 114, 797, 1031, 1278, 1375
Bme1390I CCNGG 4 cut(s) 366, 902, 972, 1043
Bme18I GGWCC 2 cut(s) 275, 1462
BmgT120I GGNCC 3 cut(s) 275, 1412, 1462
BmiI GGNNCC 7 cut(s) 6, 305, 622, 1047, 1065, 1464, 1530
BmrFI CCNGG 4 cut(s) 366, 902, 972, 1043
BmsI GCATC 3 cut(s) 21, 256, 1067
BpiI GAAGAC 1 cut(s) 659
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 1233
BpuEI CTTGAG 3 cut(s) 623, 1367, 1535
BpuMI CCSGG 1 cut(s) 366
Bsa29I ATCGAT 1 cut(s) 942
BsaBI GATNNNNATC 3 cut(s) 294, 714, 948
BsaI GGTCTC 3 cut(s) 504, 1257, 1402
BsaJI CCNNGG 1 cut(s) 552
Bsc4I CCNNNNNNNGG 6 cut(s) 284, 345, 520, 1073, 1152, 1446
Bse118I RCCGGY 1 cut(s) 837
Bse1I ACTGG 1 cut(s) 1216
Bse21I CCTNAGG 1 cut(s) 875
Bse8I GATNNNNATC 3 cut(s) 294, 714, 948
BseBI CCWGG 3 cut(s) 902, 972, 1043
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 1 cut(s) 552
BseGI GGATG 4 cut(s) 816, 850, 910, 1082
BseJI GATNNNNATC 3 cut(s) 294, 714, 948
BseLI CCNNNNNNNGG 6 cut(s) 284, 345, 520, 1073, 1152, 1446
BseMII CTCAG 2 cut(s) 313, 1413
BseNI ACTGG 1 cut(s) 1216
BseRI GAGGAG 3 cut(s) 95, 443, 1250
BseSI GKGCMC 1 cut(s) 1050
BseXI GCAGC 3 cut(s) 782, 1288, 1385
Bsh1236I CGCG 1 cut(s) 115
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 970, 1041, 1414
BshNI GGYRCC 2 cut(s) 620, 1045
BshVI ATCGAT 1 cut(s) 942
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 2 cut(s) 365, 838
BslI CCNNNNNNNGG 6 cut(s) 284, 345, 520, 1073, 1152, 1446
BsmAI GTCTC 5 cut(s) 504, 902, 1257, 1402, 1510
BsmI GAATGC 2 cut(s) 613, 1451
BsnI GGCC 3 cut(s) 970, 1041, 1414
Bso31I GGTCTC 3 cut(s) 504, 1257, 1402
Bsp1286I GDGCHC 3 cut(s) 249, 1050, 1319
BspACI CCGC 2 cut(s) 113, 1030
BspANI GGCC 3 cut(s) 970, 1041, 1414
BspCNI CTCAG 2 cut(s) 312, 1412
BspDI ATCGAT 1 cut(s) 942
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 7 cut(s) 6, 305, 622, 1047, 1065, 1464, 1530
BspMI ACCTGC 1 cut(s) 1384
BspPI GGATC 4 cut(s) 892, 1136, 1523, 1536
BspQI GCTCTTC 1 cut(s) 63
BspT107I GGYRCC 2 cut(s) 620, 1045
BspTNI GGTCTC 3 cut(s) 504, 1257, 1402
BsrFI RCCGGY 1 cut(s) 837
BsrI ACTGG 1 cut(s) 1216
BssAI RCCGGY 1 cut(s) 837
BssECI CCNNGG 1 cut(s) 552
BssNAI GTATAC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 1299
BssT1I CCWWGG 1 cut(s) 552
Bst1107I GTATAC 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 1299
Bst2UI CCWGG 3 cut(s) 902, 972, 1043
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 2 cut(s) 63, 99
BstC8I GCNNGC 5 cut(s) 163, 865, 936, 1170, 1371
BstDEI CTNAG 5 cut(s) 157, 299, 875, 1399, 1566
BstF5I GGATG 4 cut(s) 816, 850, 910, 1082
BstFNI CGCG 1 cut(s) 115
BstH2I RGCGCY 1 cut(s) 505
BstHHI GCGC 3 cut(s) 117, 504, 762
BstMAI GTCTC 5 cut(s) 504, 902, 1257, 1402, 1510
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 4 cut(s) 443, 1038, 1370, 1382
BstNI CCWGG 3 cut(s) 902, 972, 1043
BstNSI RCATGY 5 cut(s) 580, 664, 1172, 1296, 1501
BstSCI CCNGG 4 cut(s) 364, 900, 970, 1041
BstSFI CTRYAG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 1050
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 3 cut(s) 782, 1288, 1385
BstV2I GAAGAC 1 cut(s) 659
BstX2I RGATCY 4 cut(s) 561, 897, 1421, 1528
BstYI RGATCY 4 cut(s) 561, 897, 1421, 1528
BstZ17I GTATAC 1 cut(s) 351
Bsu15I ATCGAT 1 cut(s) 942
Bsu36I CCTNAGG 1 cut(s) 875
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 970, 1041, 1414
BsuTUI ATCGAT 1 cut(s) 942
BtgZI GCGATG 1 cut(s) 813
BtsCI GGATG 4 cut(s) 816, 850, 910, 1082
BtsIMutI CAGTG 1 cut(s) 1224
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 5 cut(s) 163, 865, 936, 1170, 1371
CfoI GCGC 3 cut(s) 117, 504, 762
Cfr10I RCCGGY 1 cut(s) 837
Cfr13I GGNCC 3 cut(s) 275, 1412, 1462
ClaI ATCGAT 1 cut(s) 942
CseI GACGC 4 cut(s) 44, 62, 556, 650
Csp6I GTAC 6 cut(s) 136, 378, 598, 621, 832, 1351
CviQI GTAC 6 cut(s) 136, 378, 598, 621, 832, 1351
DdeI CTNAG 5 cut(s) 157, 299, 875, 1399, 1566
DrdI GACNNNNNNGTC 1 cut(s) 661
DseDI GACNNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 2 cut(s) 968, 1039
Eam1104I CTCTTC 2 cut(s) 63, 99
EarI CTCTTC 2 cut(s) 63, 99
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 3 cut(s) 504, 1257, 1402
Eco47I GGWCC 2 cut(s) 275, 1462
Eco81I CCTNAGG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 1462
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 900, 970, 1041
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 1 cut(s) 552
FbaI TGATCA 3 cut(s) 289, 949, 1339
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 5 cut(s) 113, 796, 1030, 1277, 1374
FokI GGATG 4 cut(s) 803, 837, 917, 1089
FriOI GRGCYC 1 cut(s) 1319
Fsp4HI GCNGC 5 cut(s) 113, 796, 1030, 1277, 1374
FspBI CTAG 6 cut(s) 93, 359, 485, 821, 1035, 1365
GlaI GCGC 3 cut(s) 116, 503, 761
GluI GCNGC 5 cut(s) 113, 796, 1030, 1277, 1374
GsuI CTGGAG 1 cut(s) 1233
HaeII RGCGCY 1 cut(s) 505
HaeIII GGCC 3 cut(s) 970, 1041, 1414
HapII CCGG 2 cut(s) 365, 838
HgaI GACGC 4 cut(s) 44, 62, 556, 650
HhaI GCGC 3 cut(s) 117, 504, 762
Hin6I GCGC 3 cut(s) 115, 502, 760
HinP1I GCGC 3 cut(s) 115, 502, 760
HindIII AAGCTT 4 cut(s) 163, 205, 1476, 1553
HinfI GANTC 3 cut(s) 1190, 1302, 1433
HpaII CCGG 2 cut(s) 365, 838
HphI GGTGA 4 cut(s) 299, 319, 746, 1247
Hpy166II GTNNAC 3 cut(s) 351, 598, 1298
Hpy188I TCNGA 2 cut(s) 703, 1141
Hpy188III TCNNGA 4 cut(s) 59, 77, 496, 1299
Hpy8I GTNNAC 3 cut(s) 351, 598, 1298
Hpy99I CGWCG 2 cut(s) 572, 969
HpyAV CCTTC 8 cut(s) 18, 45, 700, 864, 1016, 1067, 1150, 1316
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 408, 611, 1393
HpyF10VI GCNNNNNNNGC 4 cut(s) 443, 1038, 1370, 1382
HpyF3I CTNAG 5 cut(s) 157, 299, 875, 1399, 1566
HspAI GCGC 3 cut(s) 115, 502, 760
KpnI GGTACC 1 cut(s) 624
Ksp22I TGATCA 3 cut(s) 289, 949, 1339
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 10, 309, 1063
Lsp1109I GCAGC 3 cut(s) 782, 1288, 1385
LweI GCATC 3 cut(s) 21, 256, 1067
MaeI CTAG 6 cut(s) 93, 359, 485, 821, 1035, 1365
MaeIII GTNAC 5 cut(s) 572, 1057, 1121, 1216, 1519
MfeI CAATTG 2 cut(s) 729, 1428
MflI RGATCY 4 cut(s) 561, 897, 1421, 1528
MhlI GDGCHC 3 cut(s) 249, 1050, 1319
MlsI TGGCCA 1 cut(s) 1041
MluCI AATT 4 cut(s) 491, 729, 869, 1428
MluNI TGGCCA 1 cut(s) 1041
MlyI GAGTC 2 cut(s) 1311, 1442
MmeI TCCRAC 1 cut(s) 1313
Mox20I TGGCCA 1 cut(s) 1041
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 3 cut(s) 678, 810, 888
MslI CAYNNNNRTG 1 cut(s) 285
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 1403
MspI CCGG 2 cut(s) 365, 838
MspR9I CCNGG 4 cut(s) 366, 902, 972, 1043
MunI CAATTG 2 cut(s) 729, 1428
Mva1269I GAATGC 2 cut(s) 613, 1451
MvaI CCWGG 3 cut(s) 902, 972, 1043
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 4 cut(s) 443, 1038, 1370, 1382
NciI CCSGG 1 cut(s) 366
NlaIV GGNNCC 7 cut(s) 6, 305, 622, 1047, 1065, 1464, 1530
NmuCI GTSAC 2 cut(s) 1216, 1519
NspI RCATGY 5 cut(s) 580, 664, 1172, 1296, 1501
OliI CACNNNNGTG 1 cut(s) 285
PaeI GCATGC 1 cut(s) 1172
PciI ACATGT 1 cut(s) 1292
PciSI GCTCTTC 1 cut(s) 63
PctI GAATGC 2 cut(s) 613, 1451
PfeI GAWTC 1 cut(s) 1190
PflMI CCANNNNNTGG 1 cut(s) 1152
PfoI TCCNGGA 1 cut(s) 900
PkrI GCNGC 5 cut(s) 114, 797, 1031, 1278, 1375
PleI GAGTC 2 cut(s) 1310, 1441
PpsI GAGTC 2 cut(s) 1310, 1441
PpuMI RGGWCCY 1 cut(s) 1462
PscI ACATGT 1 cut(s) 1292
Psp5II RGGWCCY 1 cut(s) 1462
Psp6I CCWGG 3 cut(s) 900, 970, 1041
PspGI CCWGG 3 cut(s) 900, 970, 1041
PspN4I GGNNCC 7 cut(s) 6, 305, 622, 1047, 1065, 1464, 1530
PspPI GGNCC 3 cut(s) 275, 1412, 1462
PspPPI RGGWCCY 1 cut(s) 1462
PsuI RGATCY 4 cut(s) 561, 897, 1421, 1528
PvuII CAGCTG 1 cut(s) 1403
RsaI GTAC 6 cut(s) 137, 379, 599, 622, 833, 1352
RsaNI GTAC 6 cut(s) 136, 378, 598, 621, 832, 1351
RseI CAYNNNNRTG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 678, 810, 888
SatI GCNGC 5 cut(s) 113, 796, 1030, 1277, 1374
Sau96I GGNCC 3 cut(s) 275, 1412, 1462
SchI GAGTC 2 cut(s) 1311, 1442
ScrFI CCNGG 4 cut(s) 366, 902, 972, 1043
SduI GDGCHC 3 cut(s) 249, 1050, 1319
SfaNI GCATC 3 cut(s) 21, 256, 1067
SfcI CTRYAG 1 cut(s) 505
SinI GGWCC 2 cut(s) 275, 1462
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 602, 1346, 1550
SmoI CTYRAG 3 cut(s) 602, 1346, 1550
SphI GCATGC 1 cut(s) 1172
Sse9I AATT 4 cut(s) 491, 729, 869, 1428
SsiI CCGC 2 cut(s) 113, 1030
SspMI CTAG 6 cut(s) 93, 359, 485, 821, 1035, 1365
StyD4I CCNGG 4 cut(s) 364, 900, 970, 1041
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 420, 942
TaqII GACCGA 1 cut(s) 836
TasI AATT 4 cut(s) 491, 729, 869, 1428
TauI GCSGC 2 cut(s) 115, 1032
TfiI GAWTC 1 cut(s) 1190
Tru1I TTAA 3 cut(s) 678, 810, 888
Tru9I TTAA 3 cut(s) 678, 810, 888
TscAI CASTG 1 cut(s) 1231
TseFI GTSAC 2 cut(s) 1216, 1519
TseI GCWGC 3 cut(s) 795, 1276, 1373
Tsp45I GTSAC 2 cut(s) 1216, 1519
TspGWI ACGGA 3 cut(s) 296, 1076, 1368
TspRI CASTG 1 cut(s) 1231
Van91I CCANNNNNTGG 1 cut(s) 1152
VpaK11BI GGWCC 2 cut(s) 275, 1462
XapI RAATTY 1 cut(s) 491
XceI RCATGY 5 cut(s) 580, 664, 1172, 1296, 1501
XmiI GTMKAC 1 cut(s) 350
XspI CTAG 6 cut(s) 93, 359, 485, 821, 1035, 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.