FvH4_1g26991

Ubiquitin-conjugating enzyme E2, catalytic domain homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017590
Physical Location & Seq
Reverse (-)
18779988 .. 18780661
674 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26991.t1

Sequence Viewer

Length: 534 bp
ATGATATCAGACGACAATTTCACAGAACCAGAACCAAAGTTGTACGGAGAGATAGAAGAAATACATGATGAGGTTGAGAGGAAGTTCCAAAAGTTCAAGAATTTTGACATACTTGGTCATTACCCCTATGATCATCACTTCGTTCGCAGCAGATCGGAAGACCACGTCGTATTGAAAGTGGCTGGGAATTTATCCGTGCCAGACTACCACCGGAACCTAAAGGACTTGCTGGAAACCCAAGATGTTCCCTCACTCTTTTACCATTCTTTTGGCTTTGATCTGAACCCAAACTTGCGAAAAGACGGCACCGTTTCGATCGACAAAACGCAAAAGGGCAAGAACACAATGGAGTTGCTGTCTTCACTCAAGGATCTGGTTGTGAATGACAAAACCTATATTCATTATTCGCAAAAGGGCTTGACATTAAACAGAAAGGTTTTCATGCAAACTTTCGAGGCTATGCTTAAGATGCTCGAATGGCCACCTAGGCCGTTTAAATATTTTGTGCTGGGATTTTCGAACAAGAGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

178

Amino Acids

20.82

Weight (kDa)

6.44

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017590)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26991
malus_domestica MD17G1277900.v1.1
prunus_persica Prupe.3G008800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487241
rosa_laevigata RLG00000023020
rosa_multiflora Rmu_ssc0000366.1_g000013
rosa_roxburghii Rroxscaffold_6G00394980
rosa_samantha Rh3AG280500 Rh3BG316400 Rh3CG314500 Rh3DG312000
rosa_wichuraiana Rw3G024840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AclWI GGATC 1 cut(s) 378
AcoI YGGCCR 1 cut(s) 479
AcsI RAATTY 2 cut(s) 100, 187
AfaI GTAC 1 cut(s) 44
AflII CTTAAG 1 cut(s) 464
AgsI TTSAA 2 cut(s) 97, 175
AjiI CACGTC 1 cut(s) 166
AluBI AGCT 1 cut(s) 528
AluI AGCT 1 cut(s) 528
Alw21I GWGCWC 1 cut(s) 530
AlwI GGATC 1 cut(s) 378
AoxI GGCC 2 cut(s) 479, 488
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 2 cut(s) 100, 187
AspA2I CCTAGG 1 cut(s) 485
AsuII TTCGAA 1 cut(s) 518
AvrII CCTAGG 1 cut(s) 485
BalI TGGCCA 1 cut(s) 481
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 530
BbsI GAAGAC 2 cut(s) 165, 351
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 1 cut(s) 159
BceAI ACGGC 2 cut(s) 319, 475
BclI TGATCA 1 cut(s) 130
BfaI CTAG 1 cut(s) 486
BfrI CTTAAG 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 487
BisI GCNGC 1 cut(s) 148
BlnI CCTAGG 1 cut(s) 485
BlsI GCNGC 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 215, 307
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 2 cut(s) 165, 351
Bpu14I TTCGAA 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
BseDI CCNNGG 1 cut(s) 485
BseXI GCAGC 1 cut(s) 159
BseYI CCCAGC 2 cut(s) 182, 508
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 2 cut(s) 481, 490
BshNI GGYRCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 1 cut(s) 530
BsiSI CCGG 1 cut(s) 211
BsnI GGCC 2 cut(s) 481, 490
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 530
Bsp143I GATC 5 cut(s) 130, 152, 277, 315, 370
BspANI GGCC 2 cut(s) 481, 490
BspHI TCATGA 1 cut(s) 530
BspLI GGNNCC 2 cut(s) 215, 307
BspPI GGATC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 305
BspTI CTTAAG 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 5 cut(s) 130, 152, 277, 315, 370
BssT1I CCWWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 310
BstAFI CTTAAG 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 518
BstKTI GATC 5 cut(s) 133, 155, 280, 318, 373
BstMBI GATC 5 cut(s) 130, 152, 277, 315, 370
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 3 cut(s) 469, 478, 487
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 2 cut(s) 165, 351
BstX2I RGATCY 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 2 cut(s) 481, 490
BtrI CACGTC 1 cut(s) 166
CciI TCATGA 1 cut(s) 530
Csp6I GTAC 1 cut(s) 43
CviAII CATG 3 cut(s) 65, 442, 531
CviJI RGCY 7 cut(s) 182, 273, 417, 458, 481, 490, 528
CviKI_1 RGCY 7 cut(s) 182, 273, 417, 458, 481, 490, 528
CviQI GTAC 1 cut(s) 43
DpnI GATC 5 cut(s) 132, 154, 279, 317, 372
DpnII GATC 5 cut(s) 130, 152, 277, 315, 370
DraI TTTAAA 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 479
Ecl136II GAGCTC 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 485
Eco24I GRGCYC 1 cut(s) 530
Eco32I GATATC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 528
EcoICRI GAGCTC 1 cut(s) 528
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 485
EcoT38I GRGCYC 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 3 cut(s) 68, 445, 534
FaiI YATR 7 cut(s) 66, 110, 129, 396, 443, 461, 532
FatI CATG 3 cut(s) 64, 441, 530
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 148
GsaI CCCAGC 2 cut(s) 186, 512
HaeIII GGCC 2 cut(s) 481, 490
HapII CCGG 1 cut(s) 211
Hin1II CATG 3 cut(s) 68, 445, 534
HpaII CCGG 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 10, 157, 282
Hpy188III TCNNGA 2 cut(s) 97, 531
Hpy99I CGWCG 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 1 cut(s) 445
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 478, 487
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 3 cut(s) 68, 445, 534
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 5 cut(s) 130, 152, 277, 315, 370
LpnPI CCDG 7 cut(s) 42, 168, 213, 215, 224, 359, 494
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 165
MalI GATC 5 cut(s) 132, 154, 279, 317, 372
MboI GATC 5 cut(s) 130, 152, 277, 315, 370
MboII GAAGA 3 cut(s) 68, 170, 351
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 530
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 3 cut(s) 16, 100, 187
MluNI TGGCCA 1 cut(s) 481
MnlI CCTC 4 cut(s) 64, 72, 259, 448
Mox20I TGGCCA 1 cut(s) 481
MscI TGGCCA 1 cut(s) 481
MseI TTAA 3 cut(s) 425, 465, 495
Msp20I TGGCCA 1 cut(s) 481
MspCI CTTAAG 1 cut(s) 464
MspI CCGG 1 cut(s) 211
MwoI GCNNNNNNNGC 3 cut(s) 469, 478, 487
NdeII GATC 5 cut(s) 130, 152, 277, 315, 370
NlaIII CATG 3 cut(s) 68, 445, 534
NlaIV GGNNCC 2 cut(s) 215, 307
NspV TTCGAA 1 cut(s) 518
PagI TCATGA 1 cut(s) 530
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 149
Ple19I CGATCG 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 530
PspFI CCCAGC 2 cut(s) 182, 508
PspN4I GGNNCC 2 cut(s) 215, 307
PsuI RGATCY 1 cut(s) 370
PsyI GACNNNGTC 1 cut(s) 164
PvuI CGATCG 1 cut(s) 318
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 530
SaqAI TTAA 3 cut(s) 425, 465, 495
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 5 cut(s) 130, 152, 277, 315, 370
SduI GDGCHC 1 cut(s) 530
SetI ASST 7 cut(s) 75, 168, 219, 395, 438, 487, 530
SfaNI GCATC 1 cut(s) 459
SfiI GGCCNNNNNGGCC 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 518
SmlI CTYRAG 2 cut(s) 365, 464
SmoI CTYRAG 2 cut(s) 365, 464
Sse9I AATT 3 cut(s) 16, 100, 187
SspI AATATT 1 cut(s) 500
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 530
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 168
TaqI TCGA 5 cut(s) 314, 318, 453, 474, 518
TasI AATT 3 cut(s) 16, 100, 187
Tru1I TTAA 3 cut(s) 425, 465, 495
Tru9I TTAA 3 cut(s) 425, 465, 495
TseI GCWGC 1 cut(s) 147
TspDTI ATGAA 2 cut(s) 389, 430
TspGWI ACGGA 2 cut(s) 60, 184
Tth111I GACNNNGTC 1 cut(s) 164
Vha464I CTTAAG 1 cut(s) 464
XapI RAATTY 2 cut(s) 100, 187
XmaJI CCTAGG 1 cut(s) 485
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.