MD17G1277900.v1.1

Ubiquitin-conjugating enzyme E2, catalytic domain homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017590
Physical Location & Seq
Forward (+)
33794250 .. 33795026
777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1277900.v1.1.491

Sequence Viewer

Length: 777 bp
ATGAGTGCTTACCCTCCTTATGATCATTACTTCCGCGAGAAGCCGTTTTATAATTGTATAGACGCAGTACTAGTCAAACGGATTCAAGAAGAACGCGGAATCCTAAGGCAAGGTCTCCCAAGTTCAATTTTTGTAAGGGTTTATGAATCAAGGATTGATGTCGTGAGAGCTGTGATAGTTGGTCGAGAGGGTGCTCCTTATCACCAAGGCTTGTTTTTATTTGACATATTCTTTCCTAGCAATTCTCCCACTCAGCCTCCCCAACTGTTTTACCACTCTTATGGCCTTGATTTCAACCCTAACTTGCCTAAAAGCGGCAACGTTTCGCTTGAAGGGGTTATTTTGAAACAATCTAATATATTGGAGACACTTGTTTCGATTCAACAATTGGTTCTTTTGAACGGTAAACCCTACCTCAATAGTGAGTATCCGCGACTCGGTGGAGAGAAATCCAACTCAAAGTTGCACAAAAGAGTTTTTGTGCAAACTTGTGAAGCTATGCTTAGGATGTTGAAGTGCCTGCCTATGGGTTTTGAAGATTTTGTTTTAGGGTTTTTCCGCACTAGAGCTCATAATATTCTGCTCAACTACAAAGCTTGTGCGGATCTTGAGGATCAACCAATAAAGACGTTGTTCTTCAAGCTTGTGAAGGCATTTGAAGCTAATGGAACTTATTGCCGTCATCATTATAACCAGGAGGAGTATGATTTGACATTCAAAAAAGAGGAGTTCCTTTCAGAGGAGGAGAGAACTATGGCTCGATTTTGGCTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

259

Amino Acids

30.08

Weight (kDa)

8.19

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 27 - 131 6.2e-11 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017590)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26991
malus_domestica MD17G1277900.v1.1
prunus_persica Prupe.3G008800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487241
rosa_laevigata RLG00000023020
rosa_multiflora Rmu_ssc0000366.1_g000013
rosa_roxburghii Rroxscaffold_6G00394980
rosa_samantha Rh3AG280500 Rh3BG316400 Rh3CG314500 Rh3DG312000
rosa_wichuraiana Rw3G024840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 51, 690
AccII CGCG 3 cut(s) 36, 96, 433
AciI CCGC 6 cut(s) 34, 96, 315, 431, 559, 602
AclI AACGTT 1 cut(s) 321
AclWI GGATC 2 cut(s) 612, 621
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 4 cut(s) 314, 437, 526, 739
AhlI ACTAGT 1 cut(s) 70
AjnI CCWGG 1 cut(s) 693
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 6 cut(s) 170, 497, 569, 596, 643, 662
AluI AGCT 6 cut(s) 170, 497, 569, 596, 643, 662
Alw21I GWGCWC 2 cut(s) 196, 571
Alw26I GTCTC 2 cut(s) 119, 359
AlwI GGATC 2 cut(s) 612, 621
AoxI GGCC 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AsuHPI GGTGA 1 cut(s) 194
AxyI CCTNAGG 1 cut(s) 104
BanII GRGCYC 1 cut(s) 571
Bbv12I GWGCWC 2 cut(s) 196, 571
BceAI ACGGC 2 cut(s) 28, 663
BciT130I CCWGG 1 cut(s) 695
BciVI GTATCC 1 cut(s) 438
BclI TGATCA 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 119, 359
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 3 cut(s) 71, 237, 564
BfuI GTATCC 1 cut(s) 438
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
BmcAI AGTACT 1 cut(s) 69
Bme1390I CCNGG 1 cut(s) 695
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 1 cut(s) 205
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 437, 526, 739
Bse21I CCTNAGG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 695
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 4 cut(s) 314, 437, 526, 739
BseMII CTCAG 1 cut(s) 266
BseRI GAGGAG 4 cut(s) 713, 740, 755, 758
Bsh1236I CGCG 3 cut(s) 36, 96, 433
BshFI GGCC 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 196, 571
BslI CCNNNNNNNGG 4 cut(s) 314, 437, 526, 739
BsmAI GTCTC 2 cut(s) 119, 359
BsnI GGCC 1 cut(s) 285
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 196, 571
Bsp143I GATC 3 cut(s) 22, 604, 613
BspACI CCGC 6 cut(s) 34, 96, 315, 431, 559, 602
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 3 cut(s) 36, 96, 433
BspLI GGNNCC 1 cut(s) 770
BspPI GGATC 2 cut(s) 612, 621
BspTNI GGTCTC 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 22, 604, 613
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 695
Bst4CI ACNGT 2 cut(s) 267, 404
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 3 cut(s) 104, 252, 503
BstENI CCTNNNNNAGG 1 cut(s) 737
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 3 cut(s) 36, 96, 433
BstKTI GATC 3 cut(s) 25, 607, 616
BstMAI GTCTC 2 cut(s) 119, 359
BstMBI GATC 3 cut(s) 22, 604, 613
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 693
BstUI CGCG 3 cut(s) 36, 96, 433
BstX2I RGATCY 1 cut(s) 604
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 604
Bsu36I CCTNAGG 1 cut(s) 104
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 285
BtsCI GGATG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 521
CseI GACGC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 68
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 3 cut(s) 104, 252, 503
DpnI GATC 3 cut(s) 24, 606, 615
DpnII GATC 3 cut(s) 22, 604, 613
Ecl136II GAGCTC 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 569
Eco81I CCTNAGG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 569
EcoNI CCTNNNNNAGG 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 693
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 205
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 1 cut(s) 520
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 3 cut(s) 71, 237, 564
GluI GCNGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 285
HgaI GACGC 1 cut(s) 71
HindIII AAGCTT 2 cut(s) 594, 641
HinfI GANTC 5 cut(s) 82, 99, 146, 379, 435
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 407
Hpy188I TCNGA 1 cut(s) 739
Hpy188III TCNNGA 4 cut(s) 86, 163, 185, 608
Hpy8I GTNNAC 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 326, 643
HpyCH4III ACNGT 2 cut(s) 267, 404
HpyCH4IV ACGT 2 cut(s) 321, 629
HpyCH4V TGCA 2 cut(s) 466, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 3 cut(s) 104, 252, 503
HpySE526I ACGT 2 cut(s) 321, 629
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 3 cut(s) 22, 604, 613
LmnI GCTCC 2 cut(s) 199, 774
LpnPI CCDG 3 cut(s) 533, 680, 707
MaeI CTAG 3 cut(s) 71, 237, 564
MaeII ACGT 2 cut(s) 321, 629
MalI GATC 3 cut(s) 24, 606, 615
MboI GATC 3 cut(s) 22, 604, 613
MboII GAAGA 3 cut(s) 101, 548, 628
MfeI CAATTG 1 cut(s) 386
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 196, 571
MluCI AATT 4 cut(s) 52, 126, 241, 386
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 9 cut(s) 24, 181, 267, 425, 604, 691, 718, 733, 736
MslI CAYNNNNRTG 1 cut(s) 279
MspR9I CCNGG 1 cut(s) 695
MunI CAATTG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 695
MvnI CGCG 3 cut(s) 36, 96, 433
MwoI GCNNNNNNNGC 1 cut(s) 659
NdeII GATC 3 cut(s) 22, 604, 613
NlaIV GGNNCC 1 cut(s) 770
PfeI GAWTC 4 cut(s) 82, 99, 146, 379
PkrI GCNGC 1 cut(s) 317
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PsiI TTATAA 2 cut(s) 51, 690
Psp124BI GAGCTC 1 cut(s) 571
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 693
PspGI CCWGG 1 cut(s) 693
PspN4I GGNNCC 1 cut(s) 770
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 279
SacI GAGCTC 1 cut(s) 571
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 3 cut(s) 22, 604, 613
ScaI AGTACT 1 cut(s) 69
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 695
SduI GDGCHC 2 cut(s) 196, 571
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 4 cut(s) 52, 126, 241, 386
SsiI CCGC 6 cut(s) 34, 96, 315, 431, 559, 602
SspI AATATT 1 cut(s) 577
SspMI CTAG 3 cut(s) 71, 237, 564
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 1 cut(s) 693
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 267, 404
TaiI ACGT 2 cut(s) 324, 632
TaqI TCGA 3 cut(s) 184, 377, 760
TasI AATT 4 cut(s) 52, 126, 241, 386
TatI WGTACW 1 cut(s) 67
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 4 cut(s) 82, 99, 146, 379
TspDTI ATGAA 1 cut(s) 159
TspGWI ACGGA 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 737
XspI CTAG 3 cut(s) 71, 237, 564
ZrmI AGTACT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.