RLG00000023020

Ubiquitin-conjugating enzyme E2, catalytic domain homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017590
Physical Location & Seq
Forward (+)
16741963 .. 16742961
999 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023020

Sequence Viewer

Length: 999 bp
ATGGGCATCAGGGCATTGGATAGTTTTTATGAATATGGAGGGTTTGATAATCATAGCTACACACCAAAACCAACCTCAACGATGGTACCTGACGAGCATATCCCAGAACCAGAACCAAAGTTGTACGGAGAGATCGAAGAAATACAATCTGAGGTTGAGAGAAAGTTCCAAGAGTTTAAGAACTTTGACATACTAGGTTATTACCCTAATGATCATCACTTTCTTTACAGGAGAGGAAAACCGCGTATTGAAAGTGAATGGGAATTTATCCGTGTTAGACTACCACCGGAACCCAAACTTGCTGGTTCGATTTTTGTTAGGGTTTATGACCAGAGGATTGATCTTATGAGTGTTATGATAGTTGGTCGGGAAAGTCCTCCTTATCACCAAGGCTTGTTCTTCTTTGACATATTCTTTCCCAGCAATTATCTCAAACTGCCTCAGCAGGAAACCGAGCCCCCACTCTTTTACCATTCTTTTGACCTAGATTTGAACCCGAACTTGCAAAAAAACGGCACCGTTTCGCTCGACAAAACACAAAAGTGCAAGAACACATTGGAGTTACTCTCTTCACTTAAAGATCTGGTCGTGAATAACAAATCCTACATTGATGCCGAAAAGGGCAGCAAATCAAACAGAAAGATTTTCATGCAAACTTTTGAGTCTATGCTTAAGATGCTCAAGCGCCCACCTAGGCCGTTTAAAGATTTTGTGCTGGGGTATTTTCGAACAAGAGCTCATGATATTCTTCTCAACTATAAAGCTTGTGCTGATCTTGAAGATAATGCAATGAACTCGTTATTCTTCAAGCTGGTGAGGGCATTTGAAGCCAACGGAACTTATTGCCAGCATCATTATAATCAGAAGCAGTACGATCGGGCATTGAAAGAAGAAAAGGAGCTCTTAAATCAGAGTCGGGAGAAATATGGATATGAAAATTTTTTGTTCTCTCCACCAAAGATGAACAAAGCAAAGGATTGGCTTTATTTTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

333

Amino Acids

39.34

Weight (kDa)

7.12

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 109 - 180 4.4e-06 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017590)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g26991
malus_domestica MD17G1277900.v1.1
prunus_persica Prupe.3G008800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487241
rosa_laevigata RLG00000023020
rosa_multiflora Rmu_ssc0000366.1_g000013
rosa_roxburghii Rroxscaffold_6G00394980
rosa_samantha Rh3AG280500 Rh3BG316400 Rh3CG314500 Rh3DG312000
rosa_wichuraiana Rw3G024840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 858
Acc65I GGTACC 1 cut(s) 85
AccB1I GGYRCC 2 cut(s) 85, 515
AccII CGCG 1 cut(s) 244
AciI CCGC 1 cut(s) 242
AcsI RAATTY 2 cut(s) 263, 937
AfaI GTAC 3 cut(s) 87, 125, 872
AflII CTTAAG 1 cut(s) 671
AgsI TTSAA 6 cut(s) 251, 493, 779, 808, 827, 886
AleI CACNNNNGTG 1 cut(s) 541
AluBI AGCT 5 cut(s) 57, 737, 764, 811, 901
AluI AGCT 5 cut(s) 57, 737, 764, 811, 901
Alw21I GWGCWC 2 cut(s) 739, 903
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 2 cut(s) 263, 937
Asp718I GGTACC 1 cut(s) 85
AspA2I CCTAGG 1 cut(s) 692
AspLEI GCGC 1 cut(s) 687
AsuHPI GGTGA 2 cut(s) 377, 826
AsuII TTCGAA 1 cut(s) 727
AvrII CCTAGG 1 cut(s) 692
BaeI ACNNNNGTAYC 2 cut(s) 69, 102
BanI GGYRCC 2 cut(s) 85, 515
BanII GRGCYC 3 cut(s) 459, 739, 903
Bbv12I GWGCWC 2 cut(s) 739, 903
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 636
BccI CCATC 1 cut(s) 76
BceAI ACGGC 2 cut(s) 529, 682
BcgI CGANNNNNNTGC 4 cut(s) 777, 811, 857, 891
BclI TGATCA 1 cut(s) 211
BfaI CTAG 3 cut(s) 194, 485, 693
BfoI RGCGCY 1 cut(s) 688
BfrI CTTAAG 1 cut(s) 671
BglII AGATCT 1 cut(s) 580
BisI GCNGC 1 cut(s) 625
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 1 cut(s) 626
BmiI GGNNCC 3 cut(s) 87, 291, 517
BmsI GCATC 4 cut(s) 15, 601, 666, 859
BplI GAGNNNNNCTC 2 cut(s) 551, 583
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 665
BsaJI CCNNGG 2 cut(s) 388, 692
BsaWI WCCGGW 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 795
BseDI CCNNGG 2 cut(s) 388, 692
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 2 cut(s) 141, 455
BseXI GCAGC 1 cut(s) 636
BseYI CCCAGC 2 cut(s) 419, 715
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 877
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 2 cut(s) 85, 515
BsiEI CGRYCG 1 cut(s) 877
BsiHKAI GWGCWC 2 cut(s) 739, 903
BsiSI CCGG 1 cut(s) 287
BsnI GGCC 1 cut(s) 697
Bsp119I TTCGAA 1 cut(s) 727
Bsp1286I GDGCHC 3 cut(s) 459, 739, 903
Bsp143I GATC 6 cut(s) 132, 211, 340, 580, 772, 874
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 697
BspCNI CTCAG 2 cut(s) 142, 454
BspFNI CGCG 1 cut(s) 244
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 3 cut(s) 87, 291, 517
BspT104I TTCGAA 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 85, 515
BspTI CTTAAG 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 795
BssECI CCNNGG 2 cut(s) 388, 692
BssMI GATC 6 cut(s) 132, 211, 340, 580, 772, 874
BssT1I CCWWGG 2 cut(s) 388, 692
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 574
BstAFI CTTAAG 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 848
BstDEI CTNAG 2 cut(s) 150, 441
BstFNI CGCG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 6 cut(s) 135, 214, 343, 583, 775, 877
BstMBI GATC 6 cut(s) 132, 211, 340, 580, 772, 874
BstMCI CGRYCG 1 cut(s) 877
BstMWI GCNNNNNNNGC 2 cut(s) 676, 827
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 580
BstYI RGATCY 1 cut(s) 580
BsuRI GGCC 1 cut(s) 697
Cac8I GCNNGC 1 cut(s) 848
CciI TCATGA 1 cut(s) 739
CfoI GCGC 1 cut(s) 687
Csp6I GTAC 3 cut(s) 86, 124, 871
CviAII CATG 2 cut(s) 649, 740
CviQI GTAC 3 cut(s) 86, 124, 871
DdeI CTNAG 2 cut(s) 150, 441
DpnI GATC 6 cut(s) 134, 213, 342, 582, 774, 876
DpnII GATC 6 cut(s) 132, 211, 340, 580, 772, 874
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
Ecl136II GAGCTC 2 cut(s) 737, 901
Eco130I CCWWGG 2 cut(s) 388, 692
Eco24I GRGCYC 3 cut(s) 459, 739, 903
Eco53kI GAGCTC 2 cut(s) 737, 901
EcoICRI GAGCTC 2 cut(s) 737, 901
EcoT14I CCWWGG 2 cut(s) 388, 692
EcoT38I GRGCYC 3 cut(s) 459, 739, 903
ErhI CCWWGG 2 cut(s) 388, 692
FaeI CATG 2 cut(s) 652, 743
FalI AAGNNNNNCTT 4 cut(s) 364, 396, 887, 919
FatI CATG 2 cut(s) 648, 739
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 625
FriOI GRGCYC 3 cut(s) 459, 739, 903
Fsp4HI GCNGC 1 cut(s) 625
FspBI CTAG 3 cut(s) 194, 485, 693
GlaI GCGC 1 cut(s) 686
GluI GCNGC 1 cut(s) 625
GsaI CCCAGC 2 cut(s) 423, 719
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 1 cut(s) 697
HapII CCGG 1 cut(s) 287
HhaI GCGC 1 cut(s) 687
Hin1II CATG 2 cut(s) 652, 743
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HindIII AAGCTT 1 cut(s) 762
HinfI GANTC 2 cut(s) 662, 913
HpaII CCGG 1 cut(s) 287
HphI GGTGA 2 cut(s) 377, 826
Hpy188I TCNGA 3 cut(s) 151, 864, 912
Hpy188III TCNNGA 5 cut(s) 368, 589, 740, 776, 917
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 4 cut(s) 505, 546, 652, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 676, 827
HpyF3I CTNAG 2 cut(s) 150, 441
Hsp92II CATG 2 cut(s) 652, 743
HspAI GCGC 1 cut(s) 685
KpnI GGTACC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 6 cut(s) 132, 211, 340, 580, 772, 874
LmnI GCTCC 1 cut(s) 898
Lsp1109I GCAGC 1 cut(s) 636
LweI GCATC 4 cut(s) 15, 601, 666, 859
MaeI CTAG 3 cut(s) 194, 485, 693
MaeIII GTNAC 1 cut(s) 561
MalI GATC 6 cut(s) 134, 213, 342, 582, 774, 876
MboI GATC 6 cut(s) 132, 211, 340, 580, 772, 874
MboII GAAGA 7 cut(s) 149, 391, 561, 740, 791, 796, 902
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 3 cut(s) 459, 739, 903
MluCI AATT 3 cut(s) 263, 424, 937
MlyI GAGTC 2 cut(s) 671, 922
MnlI CCTC 8 cut(s) 32, 85, 145, 227, 327, 387, 450, 810
MseI TTAA 5 cut(s) 177, 576, 672, 702, 905
MslI CAYNNNNRTG 1 cut(s) 541
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 676, 827
NdeII GATC 6 cut(s) 132, 211, 340, 580, 772, 874
NlaIII CATG 2 cut(s) 652, 743
NlaIV GGNNCC 3 cut(s) 87, 291, 517
NspV TTCGAA 1 cut(s) 727
OliI CACNNNNGTG 1 cut(s) 541
PagI TCATGA 1 cut(s) 739
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PkrI GCNGC 1 cut(s) 626
Ple19I CGATCG 1 cut(s) 877
PleI GAGTC 2 cut(s) 670, 921
PpsI GAGTC 2 cut(s) 670, 921
PsiI TTATAA 1 cut(s) 858
Psp124BI GAGCTC 2 cut(s) 739, 903
PspFI CCCAGC 2 cut(s) 419, 715
PspN4I GGNNCC 3 cut(s) 87, 291, 517
PsuI RGATCY 1 cut(s) 580
PvuI CGATCG 1 cut(s) 877
RsaI GTAC 3 cut(s) 87, 125, 872
RsaNI GTAC 3 cut(s) 86, 124, 871
RseI CAYNNNNRTG 1 cut(s) 541
SacI GAGCTC 2 cut(s) 739, 903
SaqAI TTAA 5 cut(s) 177, 576, 672, 702, 905
SatI GCNGC 1 cut(s) 625
Sau3AI GATC 6 cut(s) 132, 211, 340, 580, 772, 874
SchI GAGTC 2 cut(s) 671, 922
SduI GDGCHC 3 cut(s) 459, 739, 903
SfaNI GCATC 4 cut(s) 15, 601, 666, 859
SfuI TTCGAA 1 cut(s) 727
SmiMI CAYNNNNRTG 1 cut(s) 541
SmlI CTYRAG 2 cut(s) 671, 680
SmoI CTYRAG 2 cut(s) 671, 680
Sse9I AATT 3 cut(s) 263, 424, 937
SsiI CCGC 1 cut(s) 242
SspMI CTAG 3 cut(s) 194, 485, 693
SstI GAGCTC 2 cut(s) 739, 903
StyI CCWWGG 2 cut(s) 388, 692
TaaI ACNGT 1 cut(s) 520
TaqI TCGA 4 cut(s) 135, 308, 528, 727
TasI AATT 3 cut(s) 263, 424, 937
Tru1I TTAA 5 cut(s) 177, 576, 672, 702, 905
Tru9I TTAA 5 cut(s) 177, 576, 672, 702, 905
TseI GCWGC 1 cut(s) 624
TspDTI ATGAA 5 cut(s) 45, 637, 806, 948, 977
TspGWI ACGGA 3 cut(s) 141, 260, 849
Vha464I CTTAAG 1 cut(s) 671
XapI RAATTY 2 cut(s) 263, 937
XmaJI CCTAGG 1 cut(s) 692
XspI CTAG 3 cut(s) 194, 485, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.