FvH4_1g29750

Domain of unknown function (DUF309)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013514
Physical Location & Seq
Reverse (-)
23174244 .. 23175531
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29750.t1

Sequence Viewer

Length: 780 bp
ATGGCATTTCCAGCTTCTGCTATCTGCAGTCCAACTAGTCCTGTTGTTTCTCTCTCGTCCATCTCAAACTCTCTCGCCCCCTATCAAGCCAACCCAACCGCCCTCTTCTCTACTGATTATTGTTCAAAGCCTCTCAGCAGCTACACAAGTGAACACAGTTACAGTTGCAGGCACTACTCATCTTGTTTCCGTATTTCATATCGATCCTCTGATGCTGAAGAAGGTGAAGACGAAGCTGAAGGTTGCAGCTTTGATGAAGCAGTCGTCCTTTTCAACACAAGAGAATACTATAAGTGCCATGACTTTCTTGAGTCACTTTGGAACAAGGCTGAAGAACCCACCAGAACTCTTTTTCATGGTATTTTACAATGCGCTGTGGGATTCCACCACCTATTCAACCAGAACCATAAAGGGGCTATGATGGAGCTGGGAGAGGGACTGTGTAAGCTTAGAAAGATGAACTTCAAAACCGGGCCATTTTACGAGTTTGAGCAACAAATCTCTGCAGCTCTGGATTTCATTTACCAAACGCAAATAGAGTTAGCCGCCTGTGGAGATGATGTTTGTGTTGCGATGGATCGATCAGAGAGATCGTACCAACTTCTAGGCCGGTATGCTGCAGGACAGCTACTCTATACCCTGCAACTGCAAGCTGTTGACGACTCTGAAACCATGTACATCGTGTTCCGGCCTCAAAGGTCTTATGGCGGTGAGCCACCAAAGGTGAAGCTTCCAACTCTCGATGCTACCACACAACATCTCAGCAGTCACGGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF309

Protein Analysis

260

Amino Acids

29.06

Weight (kDa)

5.24

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF309 PF03745 84 - 140 7.1e-22 Domain of unknown function (DUF309)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41120
fragaria_vesca FvH4_1g29750
malus_domestica MD12G1013500.v1.1
prunus_persica Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1
pyrus_communis pycom12g01080
rosa_chinensis RchiOBHm_Chr3g0495771
rosa_laevigata RLG00000022587
rosa_multiflora Rmu_co8355459.1_g000001
rosa_roxburghii Rroxscaffold_6G00390020
rosa_rugosa Rorug03G0276400
rosa_samantha Rh3AG323700 Rh3BG359300 Rh3CG355900 Rh3DG359100
rosa_wichuraiana Rw3G028300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 99, 546, 708
AclWI GGATC 2 cut(s) 198, 585
AcuI CTGAAG 3 cut(s) 237, 258, 351
AfaI GTAC 2 cut(s) 596, 677
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 4 cut(s) 126, 274, 397, 466
AhlI ACTAGT 1 cut(s) 35
AlwI GGATC 2 cut(s) 198, 585
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 4 cut(s) 473, 607, 689, 772
ApeKI GCWGC 4 cut(s) 138, 246, 506, 617
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 3 cut(s) 236, 722, 736
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 150, 258, 518, 604
BccI CCATC 3 cut(s) 68, 415, 568
BcnI CCSGG 1 cut(s) 472
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 2 cut(s) 36, 605
BfmI CTRYAG 3 cut(s) 25, 504, 618
BisI GCNGC 5 cut(s) 139, 247, 507, 546, 618
BlsI GCNGC 5 cut(s) 140, 248, 508, 547, 619
Bme1390I CCNGG 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 473
BmrFI CCNGG 1 cut(s) 472
BmsI GCATC 2 cut(s) 202, 733
BpiI GAAGAC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 472
Bsa29I ATCGAT 2 cut(s) 202, 580
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 609
BseCI ATCGAT 2 cut(s) 202, 580
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 148, 775
BseXI GCAGC 4 cut(s) 150, 258, 518, 604
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 4 cut(s) 475, 609, 691, 774
BshVI ATCGAT 2 cut(s) 202, 580
BsiSI CCGG 3 cut(s) 471, 610, 688
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 4 cut(s) 475, 609, 691, 774
Bsp1407I TGTACA 1 cut(s) 675
Bsp143I GATC 4 cut(s) 203, 577, 581, 590
BspACI CCGC 3 cut(s) 99, 546, 708
BspANI GGCC 4 cut(s) 475, 609, 691, 774
BspCNI CTCAG 2 cut(s) 147, 774
BspDI ATCGAT 2 cut(s) 202, 580
BspMAI CTGCAG 3 cut(s) 29, 508, 622
BspPI GGATC 2 cut(s) 198, 585
BsrFI RCCGGY 1 cut(s) 609
BsrGI TGTACA 1 cut(s) 675
BssAI RCCGGY 1 cut(s) 609
BssMI GATC 4 cut(s) 203, 577, 581, 590
Bst4CI ACNGT 3 cut(s) 158, 164, 441
Bst6I CTCTTC 1 cut(s) 110
BstAUI TGTACA 1 cut(s) 675
BstC8I GCNNGC 2 cut(s) 170, 651
BstDEI CTNAG 3 cut(s) 134, 449, 761
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 4 cut(s) 206, 580, 584, 593
BstMBI GATC 4 cut(s) 203, 577, 581, 590
BstMWI GCNNNNNNNGC 2 cut(s) 11, 771
BstSCI CCNGG 1 cut(s) 470
BstSFI CTRYAG 3 cut(s) 25, 504, 618
BstV1I GCAGC 4 cut(s) 150, 258, 518, 604
BstV2I GAAGAC 1 cut(s) 234
Bsu15I ATCGAT 2 cut(s) 202, 580
BsuRI GGCC 4 cut(s) 475, 609, 691, 774
BsuTUI ATCGAT 2 cut(s) 202, 580
BtgZI GCGATG 1 cut(s) 587
Cac8I GCNNGC 2 cut(s) 170, 651
CaiI CAGNNNCTG 1 cut(s) 17
CfoI GCGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 609
Cfr13I GGNCC 1 cut(s) 473
ClaI ATCGAT 2 cut(s) 202, 580
Csp6I GTAC 2 cut(s) 595, 676
CviAII CATG 3 cut(s) 299, 356, 673
CviQI GTAC 2 cut(s) 595, 676
DdeI CTNAG 3 cut(s) 134, 449, 761
DpnI GATC 4 cut(s) 205, 579, 583, 592
DpnII GATC 4 cut(s) 203, 577, 581, 590
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco57I CTGAAG 3 cut(s) 237, 258, 351
FaeI CATG 3 cut(s) 302, 359, 676
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 450
FatI CATG 3 cut(s) 298, 355, 672
Fnu4HI GCNGC 5 cut(s) 139, 247, 507, 546, 618
Fsp4HI GCNGC 5 cut(s) 139, 247, 507, 546, 618
FspBI CTAG 2 cut(s) 36, 605
GlaI GCGC 1 cut(s) 373
GluI GCNGC 5 cut(s) 139, 247, 507, 546, 618
GsaI CCCAGC 1 cut(s) 431
HaeIII GGCC 4 cut(s) 475, 609, 691, 774
HapII CCGG 3 cut(s) 471, 610, 688
HhaI GCGC 1 cut(s) 374
Hin1II CATG 3 cut(s) 302, 359, 676
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 2 cut(s) 446, 728
HinfI GANTC 3 cut(s) 311, 381, 662
HpaII CCGG 3 cut(s) 471, 610, 688
HphI GGTGA 3 cut(s) 236, 722, 736
Hpy166II GTNNAC 2 cut(s) 152, 658
Hpy188I TCNGA 4 cut(s) 211, 586, 667, 779
Hpy188III TCNNGA 3 cut(s) 308, 512, 740
Hpy8I GTNNAC 2 cut(s) 152, 658
HpyAV CCTTC 2 cut(s) 215, 233
HpyCH4III ACNGT 3 cut(s) 158, 164, 441
HpyCH4V TGCA 7 cut(s) 27, 168, 246, 506, 620, 643, 649
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 771
HpyF3I CTNAG 3 cut(s) 134, 449, 761
Hsp92II CATG 3 cut(s) 302, 359, 676
HspAI GCGC 1 cut(s) 372
Kzo9I GATC 4 cut(s) 203, 577, 581, 590
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 4 cut(s) 150, 258, 518, 604
LweI GCATC 2 cut(s) 202, 733
MaeI CTAG 2 cut(s) 36, 605
MaeIII GTNAC 3 cut(s) 158, 312, 767
MalI GATC 4 cut(s) 205, 579, 583, 592
MboI GATC 4 cut(s) 203, 577, 581, 590
MboII GAAGA 4 cut(s) 97, 230, 239, 344
MlyI GAGTC 2 cut(s) 320, 656
MmeI TCCRAC 2 cut(s) 56, 758
MnlI CCTC 5 cut(s) 113, 141, 217, 427, 702
MspI CCGG 3 cut(s) 471, 610, 688
MspR9I CCNGG 1 cut(s) 472
MwoI GCNNNNNNNGC 2 cut(s) 11, 771
NciI CCSGG 1 cut(s) 472
NdeII GATC 4 cut(s) 203, 577, 581, 590
NlaIII CATG 3 cut(s) 302, 359, 676
NmuCI GTSAC 2 cut(s) 312, 767
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 5 cut(s) 140, 248, 508, 547, 619
PleI GAGTC 2 cut(s) 319, 656
PpsI GAGTC 2 cut(s) 319, 656
PspFI CCCAGC 1 cut(s) 427
PspPI GGNCC 1 cut(s) 473
PsrI GAACNNNNNNTAC 2 cut(s) 668, 700
PstI CTGCAG 3 cut(s) 29, 508, 622
PstNI CAGNNNCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 596, 677
RsaNI GTAC 2 cut(s) 595, 676
SatI GCNGC 5 cut(s) 139, 247, 507, 546, 618
Sau3AI GATC 4 cut(s) 203, 577, 581, 590
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 2 cut(s) 320, 656
ScrFI CCNGG 1 cut(s) 472
SfaNI GCATC 2 cut(s) 202, 733
SfcI CTRYAG 3 cut(s) 25, 504, 618
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SpeI ACTAGT 1 cut(s) 35
SsiI CCGC 3 cut(s) 99, 546, 708
SspMI CTAG 2 cut(s) 36, 605
StyD4I CCNGG 1 cut(s) 470
TaaI ACNGT 3 cut(s) 158, 164, 441
TaqI TCGA 3 cut(s) 202, 580, 741
TatI WGTACW 1 cut(s) 675
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 381
TseFI GTSAC 2 cut(s) 312, 767
TseI GCWGC 4 cut(s) 138, 246, 506, 617
Tsp45I GTSAC 2 cut(s) 312, 767
TspDTI ATGAA 5 cut(s) 186, 270, 344, 473, 508
TspGWI ACGGA 1 cut(s) 179
XspI CTAG 2 cut(s) 36, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.