pycom12g01080

Domain of unknown function (DUF309)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013514
Physical Location & Seq
Forward (+)
945036 .. 947170
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01080.1

Sequence Viewer

Length: 762 bp
ATGGCGTTTCCATCTGCTGCCACTCCCACTGTTTTATCTCCCTCGTCAATCTCAAGCCGTCCGATTCGACACTCTCATCTCTTCCCTTCTCAAACCAATCCAATAACTATTACCTGCAGCTTCTCTTCTCCCTCCCCATGCAGCAACACAAGGTCAATCCTAGTACCCACCAGCAGCCGGTACTACTGTTGTTTTCGGGTTTCATATAGATTTTCCGGTGCCGGTGAAGGTGAGGAGGAGGACGACGAAGAGGAGGAGTGTAGCTTTGATGAAGCCGTTGTGCTGTTCAACACAAGGGAATACTACAAGTGCCATGACTTTCTCGAATCGCTTTGGAACAATGCTGAAGAACCGTCCAGAACTCTTATTCATGGCATTCTACAATGCGCAGTGGGATTCCATCACCTATTCAATCAGAACCATAAAGGAGCGATGATGGAGTTGGGAGAAGGACTTTTTCATGAGTTTGAGCAAGAAATCTCTGCAGCCCTGGATTTTATTTACCAAACACAGATAGAGTTAGCTGCCTGTACGGATGAAGTTTGTGTTACAATGGATCAATCAGAGAGATCATACCAACTTCTAGGCCGGTATGGTGCAGGTCAGCTACTATATACCCTCCAAAGGGACCCTAATTCTAATCAATTTATGTACAATATTGTCTTCTCCCCTCACACCTCTTATGGGAGTGAGCCACTAAAGGTCAAGCTTCCTACCCTTAATGCAACCACACAACATCTCTTGGCCTGTGAATACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF309

Protein Analysis

254

Amino Acids

28.56

Weight (kDa)

4.92

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF309 PF03745 89 - 145 5.6e-22 Domain of unknown function (DUF309)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41120
fragaria_vesca FvH4_1g29750
malus_domestica MD12G1013500.v1.1
prunus_persica Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1
pyrus_communis pycom12g01080
rosa_chinensis RchiOBHm_Chr3g0495771
rosa_laevigata RLG00000022587
rosa_multiflora Rmu_co8355459.1_g000001
rosa_roxburghii Rroxscaffold_6G00390020
rosa_rugosa Rorug03G0276400
rosa_samantha Rh3AG323700 Rh3BG359300 Rh3CG355900 Rh3DG359100
rosa_wichuraiana Rw3G028300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 122, 590
AccB1I GGYRCC 1 cut(s) 218
AclWI GGATC 1 cut(s) 564
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 4 cut(s) 165, 182, 532, 653
AfiI CCNNNNNNNGG 4 cut(s) 177, 624, 625, 684
AgsI TTSAA 2 cut(s) 289, 412
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 5 cut(s) 120, 264, 524, 607, 709
AluI AGCT 5 cut(s) 120, 264, 524, 607, 709
AlwI GGATC 1 cut(s) 564
AoxI GGCC 2 cut(s) 586, 744
ApeKI GCWGC 6 cut(s) 17, 117, 141, 174, 485, 524
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 628
AsuHPI GGTGA 3 cut(s) 236, 242, 395
AvaII GGWCC 1 cut(s) 628
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 655
BbvI GCAGC 6 cut(s) 4, 129, 153, 186, 497, 511
BccI CCATC 3 cut(s) 19, 408, 430
BceAI ACGGC 2 cut(s) 42, 260
BciT130I CCWGG 1 cut(s) 491
BfaI CTAG 2 cut(s) 161, 584
BfmI CTRYAG 2 cut(s) 115, 483
BfuAI ACCTGC 2 cut(s) 122, 590
BisI GCNGC 6 cut(s) 18, 118, 142, 175, 486, 525
BlsI GCNGC 6 cut(s) 19, 119, 143, 176, 487, 526
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 3 cut(s) 220, 629, 630
BmrFI CCNGG 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 655
BpuEI CTTGAG 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 624, 625, 684
Bse118I RCCGGY 3 cut(s) 177, 221, 588
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 4 cut(s) 177, 624, 625, 684
BseRI GAGGAG 4 cut(s) 248, 251, 266, 269
BseXI GCAGC 6 cut(s) 4, 129, 153, 186, 497, 511
BsgI GTGCAG 1 cut(s) 618
BshFI GGCC 2 cut(s) 588, 746
BshNI GGYRCC 1 cut(s) 218
BsiSI CCGG 4 cut(s) 178, 216, 222, 589
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 4 cut(s) 177, 624, 625, 684
BsmFI GGGAC 1 cut(s) 641
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 2 cut(s) 588, 746
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 2 cut(s) 556, 569
BspANI GGCC 2 cut(s) 588, 746
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 220, 629, 630
BspMAI CTGCAG 2 cut(s) 119, 487
BspMI ACCTGC 2 cut(s) 122, 590
BspPI GGATC 1 cut(s) 564
BspT107I GGYRCC 1 cut(s) 218
BsrFI RCCGGY 3 cut(s) 177, 221, 588
BsrGI TGTACA 1 cut(s) 651
BssAI RCCGGY 3 cut(s) 177, 221, 588
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 2 cut(s) 556, 569
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 3 cut(s) 31, 188, 354
Bst6I CTCTTC 3 cut(s) 86, 130, 243
BstAUI TGTACA 1 cut(s) 651
BstF5I GGATG 1 cut(s) 541
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 2 cut(s) 559, 572
BstMBI GATC 2 cut(s) 556, 569
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 2 cut(s) 115, 483
BstV1I GCAGC 6 cut(s) 4, 129, 153, 186, 497, 511
BstV2I GAAGAC 1 cut(s) 655
BsuRI GGCC 2 cut(s) 588, 746
BtgZI GCGATG 1 cut(s) 446
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 27, 396
BveI ACCTGC 2 cut(s) 122, 590
CciI TCATGA 1 cut(s) 460
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 3 cut(s) 177, 221, 588
Cfr13I GGNCC 1 cut(s) 628
Csp6I GTAC 4 cut(s) 164, 181, 531, 652
CviAII CATG 4 cut(s) 138, 314, 371, 461
CviQI GTAC 4 cut(s) 164, 181, 531, 652
DpnI GATC 2 cut(s) 558, 571
DpnII GATC 2 cut(s) 556, 569
Eam1104I CTCTTC 3 cut(s) 86, 130, 243
EarI CTCTTC 3 cut(s) 86, 130, 243
Eco47I GGWCC 1 cut(s) 628
Eco57I CTGAAG 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 489
FaeI CATG 4 cut(s) 141, 317, 374, 464
FaqI GGGAC 1 cut(s) 641
FatI CATG 4 cut(s) 137, 313, 370, 460
Fnu4HI GCNGC 6 cut(s) 18, 118, 142, 175, 486, 525
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 6 cut(s) 18, 118, 142, 175, 486, 525
FspBI CTAG 2 cut(s) 161, 584
FspI TGCGCA 1 cut(s) 388
GlaI GCGC 1 cut(s) 388
GluI GCNGC 6 cut(s) 18, 118, 142, 175, 486, 525
HaeIII GGCC 2 cut(s) 588, 746
HapII CCGG 4 cut(s) 178, 216, 222, 589
HhaI GCGC 1 cut(s) 389
Hin1II CATG 4 cut(s) 141, 317, 374, 464
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 3 cut(s) 64, 326, 396
HpaII CCGG 4 cut(s) 178, 216, 222, 589
HphI GGTGA 3 cut(s) 236, 242, 395
Hpy188I TCNGA 3 cut(s) 63, 417, 565
Hpy188III TCNNGA 3 cut(s) 323, 357, 461
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 96, 221, 443
HpyCH4III ACNGT 3 cut(s) 31, 188, 354
HpyCH4V TGCA 5 cut(s) 117, 141, 485, 599, 725
Hsp92II CATG 4 cut(s) 141, 317, 374, 464
HspAI GCGC 1 cut(s) 387
KflI GGGWCCC 1 cut(s) 628
Kzo9I GATC 2 cut(s) 556, 569
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 6 cut(s) 4, 129, 153, 186, 497, 511
MaeI CTAG 2 cut(s) 161, 584
MaeIII GTNAC 1 cut(s) 547
MalI GATC 2 cut(s) 558, 571
MboI GATC 2 cut(s) 556, 569
MboII GAAGA 5 cut(s) 73, 117, 260, 359, 655
MluCI AATT 2 cut(s) 634, 644
MseI TTAA 1 cut(s) 720
MspI CCGG 4 cut(s) 178, 216, 222, 589
MspR9I CCNGG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 491
NdeII GATC 2 cut(s) 556, 569
NlaIII CATG 4 cut(s) 141, 317, 374, 464
NlaIV GGNNCC 3 cut(s) 220, 629, 630
NsbI TGCGCA 1 cut(s) 388
PagI TCATGA 1 cut(s) 460
PctI GAATGC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 64, 326, 396
PkrI GCNGC 6 cut(s) 19, 119, 143, 176, 487, 526
PpuMI RGGWCCY 1 cut(s) 628
Psp5II RGGWCCY 1 cut(s) 628
Psp6I CCWGG 1 cut(s) 489
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 3 cut(s) 220, 629, 630
PspPI GGNCC 1 cut(s) 628
PspPPI RGGWCCY 1 cut(s) 628
PstI CTGCAG 2 cut(s) 119, 487
RsaI GTAC 4 cut(s) 165, 182, 532, 653
RsaNI GTAC 4 cut(s) 164, 181, 531, 652
SaqAI TTAA 1 cut(s) 720
SatI GCNGC 6 cut(s) 18, 118, 142, 175, 486, 525
Sau3AI GATC 2 cut(s) 556, 569
Sau96I GGNCC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 491
SfcI CTRYAG 2 cut(s) 115, 483
SinI GGWCC 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 634, 644
SspI AATATT 1 cut(s) 658
SspMI CTAG 2 cut(s) 161, 584
StyD4I CCNGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 31, 188, 354
TaqI TCGA 2 cut(s) 67, 324
TasI AATT 2 cut(s) 634, 644
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 3 cut(s) 64, 326, 396
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TscAI CASTG 2 cut(s) 34, 396
TseI GCWGC 6 cut(s) 17, 117, 141, 174, 485, 524
TspDTI ATGAA 5 cut(s) 192, 285, 359, 449, 552
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 34, 396
VpaK11BI GGWCC 1 cut(s) 628
XspI CTAG 2 cut(s) 161, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.