Rh3BG359300

Domain of unknown function (DUF309)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013514
Physical Location & Seq
Forward (+)
43133469 .. 43135687
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG359300.1

Sequence Viewer

Length: 579 bp
ATGGCGTTTCCAGCTTCTGCTATCTGCAGTTTAACTAGTCCTGTTTTTTCTCGCTCATCGACCTCAAACTCTCTCCTCCCTTCTCAAACCAACCCAATCACCGTCTGCTCTTCTGAATATTATTCAAAGCCTTTCAGCAGCTACACAAGTCAACACAGCTGCAGGCACTACTCGTCTTGTTTTCGTATTTCATATCGATTCTCAGATGCTGGAGAAGGTGAAGACGAAGCTGAGGATTGCAGCTTTGATGAAGCAGTGGTCCTTTTCAACACAAGAGAATACTACAAGTGCCATGACTTTCTTGAGTCACTTTGGAACAAAGCTGAAGAACCCACCAGAACTCTTTTTCACGGTATTCTACAATGCGCCGTGGGATTCCATCACCTATTCAACCAGAACCATAAAGGAGCTATGATGGAGCTGGGAGAGGGACTGTGTAAGCTTAGAAAGATGAATTTCAAAACTGGGCCATTTTACGAGTTTGAGCAACAAATCTCTGCAGCTCTCGATTTCATTTACCAAACACAAATAGAGTTAGCCGCCTGTGAGTCTCTTATCTCTAAATTGAAATCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF309

Protein Analysis

192

Amino Acids

21.83

Weight (kDa)

5.62

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF309 PF03745 82 - 138 4.1e-22 Domain of unknown function (DUF309)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41120
fragaria_vesca FvH4_1g29750
malus_domestica MD12G1013500.v1.1
prunus_persica Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1
pyrus_communis pycom12g01080
rosa_chinensis RchiOBHm_Chr3g0495771
rosa_laevigata RLG00000022587
rosa_multiflora Rmu_co8355459.1_g000001
rosa_roxburghii Rroxscaffold_6G00390020
rosa_rugosa Rorug03G0276400
rosa_samantha Rh3AG323700 Rh3BG359300 Rh3CG355900 Rh3DG359100
rosa_wichuraiana Rw3G028300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 540
AcsI RAATTY 1 cut(s) 454
AcuI CTGAAG 1 cut(s) 345
AgsI TTSAA 5 cut(s) 126, 268, 391, 460, 568
AhlI ACTAGT 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 555
AlwNI CAGNNNCTG 2 cut(s) 17, 209
AoxI GGCC 1 cut(s) 467
ApeKI GCWGC 4 cut(s) 138, 159, 240, 500
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 259, 467
AsuHPI GGTGA 3 cut(s) 91, 230, 374
AvaII GGWCC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 146, 150, 252, 512
BccI CCATC 2 cut(s) 387, 409
BceAI ACGGC 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 555
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 3 cut(s) 25, 160, 498
BisI GCNGC 5 cut(s) 139, 160, 241, 501, 540
BlsI GCNGC 5 cut(s) 140, 161, 242, 502, 541
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 259, 467
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 369
Bse1I ACTGG 1 cut(s) 469
BseCI ATCGAT 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 216, 222
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 4 cut(s) 146, 150, 252, 512
BseYI CCCAGC 1 cut(s) 421
BshFI GGCC 1 cut(s) 469
BshVI ATCGAT 1 cut(s) 196
BslFI GGGAC 1 cut(s) 444
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 1 cut(s) 469
BspACI CCGC 1 cut(s) 540
BspANI GGCC 1 cut(s) 469
BspCNI CTCAG 2 cut(s) 215, 223
BspDI ATCGAT 1 cut(s) 196
BspMAI CTGCAG 3 cut(s) 29, 164, 502
BspQI GCTCTTC 1 cut(s) 115
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 103, 353, 435
Bst6I CTCTTC 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 3 cut(s) 202, 231, 443
BstDSI CCRYGG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstMAI GTCTC 1 cut(s) 555
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSFI CTRYAG 3 cut(s) 25, 160, 498
BstV1I GCAGC 4 cut(s) 146, 150, 252, 512
BstV2I GAAGAC 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 196
BsuRI GGCC 1 cut(s) 469
BsuTUI ATCGAT 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 164
CaiI CAGNNNCTG 2 cut(s) 17, 209
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 259, 467
ClaI ATCGAT 1 cut(s) 196
CviAII CATG 1 cut(s) 293
DdeI CTNAG 3 cut(s) 202, 231, 443
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 345
FaeI CATG 1 cut(s) 296
FaiI YATR 4 cut(s) 193, 294, 402, 413
FaqI GGGAC 1 cut(s) 444
FatI CATG 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 139, 160, 241, 501, 540
Fsp4HI GCNGC 5 cut(s) 139, 160, 241, 501, 540
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 367
GluI GCNGC 5 cut(s) 139, 160, 241, 501, 540
GsaI CCCAGC 1 cut(s) 425
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 469
HhaI GCGC 1 cut(s) 368
Hin1II CATG 1 cut(s) 296
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 198, 305, 375, 548
HphI GGTGA 3 cut(s) 91, 230, 374
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 2 cut(s) 115, 205
Hpy188III TCNNGA 2 cut(s) 302, 506
Hpy8I GTNNAC 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 90, 209
HpyCH4III ACNGT 3 cut(s) 103, 353, 435
HpyCH4V TGCA 4 cut(s) 27, 162, 240, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 202, 231, 443
Hsp92II CATG 1 cut(s) 296
HspAI GCGC 1 cut(s) 366
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 407, 418
LpnPI CCDG 9 cut(s) 24, 54, 148, 195, 349, 407, 407, 450, 556
Lsp1109I GCAGC 4 cut(s) 146, 150, 252, 512
LweI GCATC 1 cut(s) 196
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 306
MboII GAAGA 3 cut(s) 102, 233, 338
MluCI AATT 2 cut(s) 454, 563
MlyI GAGTC 2 cut(s) 314, 557
MnlI CCTC 4 cut(s) 73, 86, 226, 421
MseI TTAA 2 cut(s) 32, 577
MspA1I CMGCKG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 11
NlaIII CATG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 198, 375
PkrI GCNGC 5 cut(s) 140, 161, 242, 502, 541
PleI GAGTC 2 cut(s) 313, 556
PpsI GAGTC 2 cut(s) 313, 556
PspFI CCCAGC 1 cut(s) 421
PspPI GGNCC 2 cut(s) 259, 467
PstI CTGCAG 3 cut(s) 29, 164, 502
PstNI CAGNNNCTG 2 cut(s) 17, 209
PvuII CAGCTG 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 2 cut(s) 32, 577
SatI GCNGC 5 cut(s) 139, 160, 241, 501, 540
Sau96I GGNCC 2 cut(s) 259, 467
SchI GAGTC 2 cut(s) 314, 557
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 3 cut(s) 25, 160, 498
SinI GGWCC 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 2 cut(s) 454, 563
SsiI CCGC 1 cut(s) 540
SspI AATATT 1 cut(s) 119
SspMI CTAG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 103, 353, 435
TaqI TCGA 3 cut(s) 59, 196, 507
TasI AATT 2 cut(s) 454, 563
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 2 cut(s) 198, 375
Tru1I TTAA 2 cut(s) 32, 577
Tru9I TTAA 2 cut(s) 32, 577
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 4 cut(s) 138, 159, 240, 500
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 4 cut(s) 180, 264, 467, 502
TspRI CASTG 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 454
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.