MD12G1013500.v1.1

Domain of unknown function (DUF309)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013514
Physical Location & Seq
Forward (+)
1422280 .. 1424450
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1013500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGCGTTACCATCTGCTGCCACTCCCACTGTTTTATCTCCCTCGTCAATCTCAAGCCGTCCGATTCGACACTCTCATCTCTTCCCTTCCCAAACCAATCCAATAACTATCACCTGCAGCTTCTCTTCTCCCTCCCCATGCAGCAACACAAGATCAATCCTAGTACCCACCAGCAGCCGGTACTACTGTTGTTTCGGGGTTTCATATAGATTTTCCGGTGCCGGTGAAGGTGAGGAGGAGGAGGACGAAGAGGAGTGTAGCTTTGATGAAGCAGTTGTGCTGTTCAACACAAGGGAATACTACAAGTGCCATGACTTTCTCGAATCGCTTTGGAACAATGCCGAAGAACCGTCCAGAACTCTTATTCATGGCATTCTACAATGCGCAGTGGGATTCCATCACCTATTCAATCAGAACCATAAAGGAGCGATGATGGAGTTGGGAGAAGGACTTTGTAAGCTAAGAAAGATGAATTTTAAAAGTGGATCACCATTTCATGAGTTTGAGCAAGAAATCTCTGCAGCCCTGGATTTTATTTACCAAACGCAGATAGAGTTAGCTGCCTGTACGGATGAAGTTTGTGTTACAATGGATCAATCAGAGAGATCATACCAACTTCTAGGCCGGTATGGTGCAGGTCAGCTACTATATACCCTCCAAAGGGACCCTAATTCTAATCGATTTATGTACAATATTGTCTTCTCCCCTCACACCTCTTATGGGGGTGAGCCACTAAAGGTCAAGCTTCCCACCCTTAATGCAACCACACAACATCTCTTGGCCTGTGAATACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF309

Protein Analysis

266

Amino Acids

29.79

Weight (kDa)

5.27

Isoelectric Point (pI)

61.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF309 PF03745 88 - 144 6.1e-22 Domain of unknown function (DUF309)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41120
fragaria_vesca FvH4_1g29750
malus_domestica MD12G1013500.v1.1
prunus_persica Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1 Prupe.7G017900_v2.0.a1
pyrus_communis pycom12g01080
rosa_chinensis RchiOBHm_Chr3g0495771
rosa_laevigata RLG00000022587
rosa_multiflora Rmu_co8355459.1_g000001
rosa_roxburghii Rroxscaffold_6G00390020
rosa_rugosa Rorug03G0276400
rosa_samantha Rh3AG323700 Rh3BG359300 Rh3CG355900 Rh3DG359100
rosa_wichuraiana Rw3G028300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 385
Acc36I ACCTGC 2 cut(s) 122, 626
AccB1I GGYRCC 1 cut(s) 218
AclWI GGATC 2 cut(s) 493, 600
AcsI RAATTY 1 cut(s) 472
AfaI GTAC 4 cut(s) 165, 182, 568, 689
AfiI CCNNNNNNNGG 4 cut(s) 177, 660, 661, 720
AgsI TTSAA 2 cut(s) 286, 409
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 6 cut(s) 120, 261, 460, 560, 643, 745
AluI AGCT 6 cut(s) 120, 261, 460, 560, 643, 745
AlwI GGATC 2 cut(s) 493, 600
AoxI GGCC 2 cut(s) 622, 780
ApeKI GCWGC 6 cut(s) 17, 117, 141, 174, 521, 560
ApoI RAATTY 1 cut(s) 472
AspLEI GCGC 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 664
AsuHPI GGTGA 6 cut(s) 103, 236, 242, 392, 480, 737
AvaII GGWCC 1 cut(s) 664
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 691
BbvI GCAGC 6 cut(s) 4, 129, 153, 186, 533, 547
BccI CCATC 3 cut(s) 19, 405, 427
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 527
BfaI CTAG 2 cut(s) 161, 620
BfmI CTRYAG 2 cut(s) 115, 519
BfuAI ACCTGC 2 cut(s) 122, 626
BisI GCNGC 6 cut(s) 18, 118, 142, 175, 522, 561
BlsI GCNGC 6 cut(s) 19, 119, 143, 176, 523, 562
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 664
BmiI GGNNCC 3 cut(s) 220, 665, 666
BmrFI CCNGG 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 691
BpuEI CTTGAG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 679
BsaJI CCNNGG 1 cut(s) 525
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 660, 661, 720
Bse118I RCCGGY 3 cut(s) 177, 221, 624
BseBI CCWGG 1 cut(s) 527
BseCI ATCGAT 1 cut(s) 679
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 4 cut(s) 177, 660, 661, 720
BseRI GAGGAG 4 cut(s) 248, 251, 254, 266
BseXI GCAGC 6 cut(s) 4, 129, 153, 186, 533, 547
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 2 cut(s) 624, 782
BshNI GGYRCC 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 679
BsiSI CCGG 4 cut(s) 178, 216, 222, 625
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 4 cut(s) 177, 660, 661, 720
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 2 cut(s) 624, 782
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 4 cut(s) 152, 485, 592, 605
BspANI GGCC 2 cut(s) 624, 782
BspDI ATCGAT 1 cut(s) 679
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 220, 665, 666
BspMAI CTGCAG 2 cut(s) 119, 523
BspMI ACCTGC 2 cut(s) 122, 626
BspPI GGATC 2 cut(s) 493, 600
BspT107I GGYRCC 1 cut(s) 218
BsrFI RCCGGY 3 cut(s) 177, 221, 624
BsrGI TGTACA 1 cut(s) 687
BssAI RCCGGY 3 cut(s) 177, 221, 624
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 4 cut(s) 152, 485, 592, 605
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 3 cut(s) 31, 188, 351
Bst6I CTCTTC 3 cut(s) 86, 130, 243
BstAUI TGTACA 1 cut(s) 687
BstDEI CTNAG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 577
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 4 cut(s) 155, 488, 595, 608
BstMBI GATC 4 cut(s) 152, 485, 592, 605
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 2 cut(s) 115, 519
BstV1I GCAGC 6 cut(s) 4, 129, 153, 186, 533, 547
BstV2I GAAGAC 1 cut(s) 691
Bsu15I ATCGAT 1 cut(s) 679
BsuRI GGCC 2 cut(s) 624, 782
BsuTUI ATCGAT 1 cut(s) 679
BtgZI GCGATG 1 cut(s) 443
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 27, 393
BveI ACCTGC 2 cut(s) 122, 626
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 3 cut(s) 177, 221, 624
Cfr13I GGNCC 1 cut(s) 664
ClaI ATCGAT 1 cut(s) 679
Csp6I GTAC 4 cut(s) 164, 181, 567, 688
CviAII CATG 4 cut(s) 138, 311, 368, 497
CviQI GTAC 4 cut(s) 164, 181, 567, 688
DdeI CTNAG 1 cut(s) 461
DpnI GATC 4 cut(s) 154, 487, 594, 607
DpnII GATC 4 cut(s) 152, 485, 592, 605
DraI TTTAAA 1 cut(s) 478
Eam1104I CTCTTC 3 cut(s) 86, 130, 243
EarI CTCTTC 3 cut(s) 86, 130, 243
Eco47I GGWCC 1 cut(s) 664
EcoO109I RGGNCCY 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 525
FaeI CATG 4 cut(s) 141, 314, 371, 500
FaqI GGGAC 1 cut(s) 677
FatI CATG 4 cut(s) 137, 310, 367, 496
Fnu4HI GCNGC 6 cut(s) 18, 118, 142, 175, 522, 561
FokI GGATG 1 cut(s) 584
Fsp4HI GCNGC 6 cut(s) 18, 118, 142, 175, 522, 561
FspBI CTAG 2 cut(s) 161, 620
FspI TGCGCA 1 cut(s) 385
GlaI GCGC 1 cut(s) 385
GluI GCNGC 6 cut(s) 18, 118, 142, 175, 522, 561
HaeIII GGCC 2 cut(s) 624, 782
HapII CCGG 4 cut(s) 178, 216, 222, 625
HhaI GCGC 1 cut(s) 386
Hin1II CATG 4 cut(s) 141, 314, 371, 500
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 3 cut(s) 64, 323, 393
HpaII CCGG 4 cut(s) 178, 216, 222, 625
HphI GGTGA 6 cut(s) 103, 236, 242, 392, 480, 737
Hpy188I TCNGA 3 cut(s) 63, 414, 601
Hpy188III TCNNGA 3 cut(s) 320, 354, 497
HpyAV CCTTC 3 cut(s) 96, 221, 440
HpyCH4III ACNGT 3 cut(s) 31, 188, 351
HpyCH4V TGCA 5 cut(s) 117, 141, 521, 635, 761
HpyF3I CTNAG 1 cut(s) 461
Hsp92II CATG 4 cut(s) 141, 314, 371, 500
HspAI GCGC 1 cut(s) 384
KflI GGGWCCC 1 cut(s) 664
Kzo9I GATC 4 cut(s) 152, 485, 592, 605
LmnI GCTCC 1 cut(s) 425
Lsp1109I GCAGC 6 cut(s) 4, 129, 153, 186, 533, 547
MaeI CTAG 2 cut(s) 161, 620
MaeIII GTNAC 2 cut(s) 6, 583
MalI GATC 4 cut(s) 154, 487, 594, 607
MboI GATC 4 cut(s) 152, 485, 592, 605
MboII GAAGA 5 cut(s) 73, 117, 260, 356, 691
MluCI AATT 2 cut(s) 472, 670
MseI TTAA 2 cut(s) 477, 756
MspI CCGG 4 cut(s) 178, 216, 222, 625
MspR9I CCNGG 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 372
MvaI CCWGG 1 cut(s) 527
NdeII GATC 4 cut(s) 152, 485, 592, 605
NlaIII CATG 4 cut(s) 141, 314, 371, 500
NlaIV GGNNCC 3 cut(s) 220, 665, 666
NsbI TGCGCA 1 cut(s) 385
PagI TCATGA 1 cut(s) 496
PaqCI CACCTGC 1 cut(s) 122
PctI GAATGC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 64, 323, 393
PkrI GCNGC 6 cut(s) 19, 119, 143, 176, 523, 562
PpuMI RGGWCCY 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 3 cut(s) 220, 665, 666
PspPI GGNCC 1 cut(s) 664
PspPPI RGGWCCY 1 cut(s) 664
PstI CTGCAG 2 cut(s) 119, 523
RsaI GTAC 4 cut(s) 165, 182, 568, 689
RsaNI GTAC 4 cut(s) 164, 181, 567, 688
SaqAI TTAA 2 cut(s) 477, 756
SatI GCNGC 6 cut(s) 18, 118, 142, 175, 522, 561
Sau3AI GATC 4 cut(s) 152, 485, 592, 605
Sau96I GGNCC 1 cut(s) 664
ScrFI CCNGG 1 cut(s) 527
SfcI CTRYAG 2 cut(s) 115, 519
SinI GGWCC 1 cut(s) 664
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 472, 670
SspI AATATT 1 cut(s) 694
SspMI CTAG 2 cut(s) 161, 620
StyD4I CCNGG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 31, 188, 351
TaqI TCGA 3 cut(s) 67, 321, 679
TasI AATT 2 cut(s) 472, 670
TatI WGTACW 1 cut(s) 687
TfiI GAWTC 3 cut(s) 64, 323, 393
Tru1I TTAA 2 cut(s) 477, 756
Tru9I TTAA 2 cut(s) 477, 756
TscAI CASTG 2 cut(s) 34, 393
TseI GCWGC 6 cut(s) 17, 117, 141, 174, 521, 560
TspDTI ATGAA 6 cut(s) 192, 282, 356, 485, 485, 588
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 2 cut(s) 34, 393
VpaK11BI GGWCC 1 cut(s) 664
XapI RAATTY 1 cut(s) 472
XspI CTAG 2 cut(s) 161, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.