FvH4_1g30060

Nicastrin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012086
Physical Location & Seq
Forward (+)
23801115 .. 23813151
12037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30060.t1

Sequence Viewer

Length: 2007 bp
ATGGCCCCCAAATTGCTTCTGCTGCTATTGGCGTTGAGCTTTCAGCTTCATTTGTCTTTCTCCGGAGAGACTAGCATGATGGAGTCAGTTCCTGATCTCCAGAGTTCTATGTATATGATGCTTGACGGATATCCTTGTGTTCGACTACTCAATCTTTCTGGGGAGATTGGTTGTTCGAATCCTGGAAGAGAGAAGGTTGTTGCTCCAATTATCAGACTGAATAAATCCAATGAGCTATCTCAGTTATCTGCAGTTTTGCTTGCAGTGGATGAAATTGAAAGCTTTTTTGCCAGAATATTGAATGATACGACGTTTGCTAGCAACATTGCTGGTGTATTGGTTGAATCAGAGCCTGATATTCAAAATAAGTTAGAAGGATTCTCTCCAGCTTTGAAGTTCCCACAATCAAATTTTGCTCCTTACAGAAGCATCAACTATGAATGGAACCCAATTGGAACTGGTATAATGTGGAACACGTATAACTTTCCTGTATTCTTACTCTCCAAGAGTAGCACATTGACCTTGCAGCAGGCTGCCTTAAACAATGAGAAGAGCAAGAAATCTTATACTGCCGATGTTGCTGAGTTTGATTTGGTTATGCAGACAATGAAAGCTGGCACTCATGATTCAGAATCTTGCTTAGAGGAAAATACATGCCTTCCTTTAGGTGGATACAGTGTTTGGTCATCGCTTCCCCCAATCAATATTTCATCAGCAGAACAGTCCAAGCCCATAGTACTTGCAGCAGCATCAATGGATTCGGCATCCTTTTTTCGTGATAAAAACCTTGGTGCTGACTCCCCGATATCTGGACTGATTTCATTGCTGGCAGCAGTTGATGCACTTTCTAAGGGGGATATTGTGGATGACTTAAATAAACAGCTTGTATTTATTGTTTTCACCGGAGAGGCCTGGGGTTACCTTGGCAGCAGGAGATTTTTGCTGGAACTCGATCTGCAGTCTGATGCTGTTAGTGGCCTTAACTACTCATTGATTGAGACGGTTGTCGAAATTGGTTCCATTGGGAAGGTCCTCAACCAAGAAGCTAAGAACTTCTTCGTTCATACAGCTGGGACTTCCTCTGCCACAAATGCGACACTAGATGCTTTGAGGACTGCACAAGATTCAGTCAAGTCTGACCATATTGTTGTCTTGTCTGCAAATGCTTCAAATCCTGGGATACCTCCATCTTCCTTGATGGCTTTTCTGAGAAAGGACTCTCTAACCTCTGGGGTTGTATTGGAAGACTTTGATACTGTCTTCACAAACAAGTTCTACCACAGTCACCTTGATGATTTATCAAATATAAACTCCTCAGCAATTGTCGCGGCTGCTTCCCTTGTAGCACGCACCCTTTACATTCTTTCCAGTGGCGATAAAAATACAAGCAGTTCTGCTTTAACATCCATCAATGTGAATGTTTCACTGGTTGAAGAACTAATGGGTTGCCTATTAGGCTGTGAGCCAGGTCTTTCTTGTGAGCTGGTAAAAAATTATATAACACCAGCGACTACCTGCCCCAGCAACTATGTTGGCGTTATCCTTGATGAACCTTCATCCACCCCGTATATTGGATATGTTAGTGATATTTCAAGGTTTGTTTGGAACTTCCTGGCTGAAAAAACTTCTATCTCGAAGGAAAATGCTACCTCTGTTTGTTCTAAAGATTGCAATAATGATGGTAAAGTCTGCATCGGAGCAGAGACATATGGAAAGGGAGTCTGTGTCGTCTCCACAACCAGATATGTTCCAGCTTACTCAACACGGTTGAAATTTGGATCTGGATCCTGGACTGTATTGCCTCCAAATAACTCTGATCCCATGGGACTGTTGGATGCCGTTTGGACAGAAAGTAACTGGAATACAATTGGTCTCCGAGTCTATACTGTGCAAAATTCTTCGTTCGATAATATTGTTTTACTTGGGAGCATCGCCATTACAGTCTTGGCTTACCTCGCAATAGTGATTACTAAAGCCTTTTTAACAAAGGCTTTAAAGCGTGATTGA

Protein Analysis

669

Amino Acids

72.11

Weight (kDa)

4.84

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ncstrn_small PF18266 45 - 202 7.8e-43 Nicastrin small lobe
Peptidase_M28 PF04389 243 - 450 2.1e-08 Peptidase family M28
Nicastrin PF05450 244 - 453 2.7e-74 Nicastrin large lobe
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52640 AT3G52640 AT3G52640
fragaria_vesca FvH4_1g30060
malus_domestica MD17G1267200.v1.1
prunus_persica Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis pycom17g26630
rosa_chinensis RchiOBHm_Chr3g0492061
rosa_laevigata RLG00000022797
rosa_multiflora Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii Rroxscaffold_6G00392600
rosa_rugosa Rorug03G0251500
rosa_samantha Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana Rw3G026630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1523
AccII CGCG 1 cut(s) 1328
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 1 cut(s) 1328
AclWI GGATC 4 cut(s) 1779, 1786, 1792, 1811
AcsI RAATTY 3 cut(s) 409, 1772, 1894
AfaI GTAC 1 cut(s) 738
AfiI CCNNNNNNNGG 3 cut(s) 668, 809, 1571
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 9 cut(s) 278, 301, 344, 362, 394, 1170, 1433, 1593, 1771
AjnI CCWGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
Alw26I GTCTC 5 cut(s) 62, 992, 1697, 1735, 1877
AlwI GGATC 4 cut(s) 1779, 1786, 1792, 1811
AlwNI CAGNNNCTG 2 cut(s) 92, 353
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 3 cut(s) 3, 909, 976
ApeKI GCWGC 8 cut(s) 22, 526, 533, 743, 746, 830, 927, 1331
ApoI RAATTY 3 cut(s) 409, 1772, 1894
Asp700I GAANNNNTTC 1 cut(s) 1055
AspS9I GGNCC 2 cut(s) 4, 1030
AsuHPI GGTGA 2 cut(s) 892, 1277
AsuII TTCGAA 1 cut(s) 176
AsuNHI GCTAGC 1 cut(s) 317
AvaII GGWCC 1 cut(s) 1030
BamHI GGATCC 1 cut(s) 1784
BbsI GAAGAC 2 cut(s) 1251, 1252
BbvCI CCTCAGC 1 cut(s) 1315
BbvI GCAGC 8 cut(s) 9, 520, 538, 755, 758, 842, 939, 1318
BccI CCATC 5 cut(s) 73, 1192, 1195, 1415, 1673
BceAI ACGGC 1 cut(s) 1823
BciT130I CCWGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
BciVI GTATCC 2 cut(s) 665, 1173
BcoDI GTCTC 5 cut(s) 62, 992, 1697, 1735, 1877
BfaI CTAG 3 cut(s) 72, 318, 1100
BfmI CTRYAG 2 cut(s) 249, 956
BfuAI ACCTGC 1 cut(s) 1523
BfuI GTATCC 2 cut(s) 665, 1173
BglI GCCNNNNNGGC 1 cut(s) 1455
BisI GCNGC 9 cut(s) 23, 527, 534, 744, 747, 831, 928, 1329, 1332
BlsI GCNGC 9 cut(s) 24, 528, 535, 745, 748, 832, 929, 1330, 1333
BmcAI AGTACT 1 cut(s) 738
Bme1390I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
Bme18I GGWCC 1 cut(s) 1030
BmgT120I GGNCC 2 cut(s) 4, 1030
BmiI GGNNCC 4 cut(s) 6, 446, 1018, 1786
BmrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
BmtI GCTAGC 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 1004
BpiI GAAGAC 2 cut(s) 1251, 1252
BpmI CTGGAG 2 cut(s) 83, 369
Bpu10I CCTNAGC 1 cut(s) 1315
Bpu14I TTCGAA 1 cut(s) 176
BsaAI YACGTR 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 1783
BsaI GGTCTC 1 cut(s) 1877
BsaJI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1821
BsaWI WCCGGW 2 cut(s) 62, 902
BsaXI ACNNNNNCTCC 2 cut(s) 1710, 1740
Bsc4I CCNNNNNNNGG 3 cut(s) 668, 809, 1571
Bse1I ACTGG 4 cut(s) 463, 1368, 1431, 1862
Bse3DI GCAATG 2 cut(s) 324, 821
Bse8I GATNNNNATC 1 cut(s) 1783
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
BseDI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1821
BseGI GGATG 6 cut(s) 274, 764, 871, 1403, 1556, 1840
BseJI GATNNNNATC 1 cut(s) 1783
BseLI CCNNNNNNNGG 3 cut(s) 668, 809, 1571
BseMI GCAATG 2 cut(s) 324, 821
BseMII CTCAG 4 cut(s) 254, 573, 1199, 1329
BseNI ACTGG 4 cut(s) 463, 1368, 1431, 1862
BseRI GAGGAG 1 cut(s) 1303
BseXI GCAGC 8 cut(s) 9, 520, 538, 755, 758, 842, 939, 1318
BseYI CCCAGC 2 cut(s) 1070, 1520
BsgI GTGCAG 1 cut(s) 1101
Bsh1236I CGCG 1 cut(s) 1328
BshFI GGCC 3 cut(s) 5, 911, 978
BsiSI CCGG 2 cut(s) 63, 903
BslFI GGGAC 2 cut(s) 1087, 1839
BslI CCNNNNNNNGG 3 cut(s) 668, 809, 1571
BsmAI GTCTC 5 cut(s) 62, 992, 1697, 1735, 1877
BsmBI CGTCTC 2 cut(s) 992, 1735
BsmFI GGGAC 2 cut(s) 1087, 1839
BsnI GGCC 3 cut(s) 5, 911, 978
Bso31I GGTCTC 1 cut(s) 1877
Bsp119I TTCGAA 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 5 cut(s) 94, 952, 1778, 1784, 1816
Bsp19I CCATGG 1 cut(s) 1821
BspACI CCGC 1 cut(s) 1328
BspANI GGCC 3 cut(s) 5, 911, 978
BspCNI CTCAG 4 cut(s) 253, 574, 1200, 1328
BspEI TCCGGA 1 cut(s) 62
BspFNI CGCG 1 cut(s) 1328
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 4 cut(s) 6, 446, 1018, 1786
BspMAI CTGCAG 2 cut(s) 253, 960
BspMI ACCTGC 1 cut(s) 1523
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 4 cut(s) 1779, 1786, 1792, 1811
BspQI GCTCTTC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 1877
BsrDI GCAATG 2 cut(s) 324, 821
BsrI ACTGG 4 cut(s) 463, 1368, 1431, 1862
BssECI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1821
BssMI GATC 5 cut(s) 94, 952, 1778, 1784, 1816
BssT1I CCWWGG 3 cut(s) 787, 922, 1821
Bst2UI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
Bst6I CTCTTC 2 cut(s) 181, 545
BstAPI GCANNNNNTGC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 6 cut(s) 261, 319, 531, 616, 828, 1348
BstDEI CTNAG 7 cut(s) 240, 582, 640, 849, 1047, 1208, 1315
BstDSI CCRYGG 1 cut(s) 1821
BstEII GGTNACC 1 cut(s) 917
BstF5I GGATG 6 cut(s) 274, 764, 871, 1403, 1556, 1840
BstFNI CGCG 1 cut(s) 1328
BstKTI GATC 5 cut(s) 97, 955, 1781, 1787, 1819
BstMAI GTCTC 5 cut(s) 62, 992, 1697, 1735, 1877
BstMBI GATC 5 cut(s) 94, 952, 1778, 1784, 1816
BstMWI GCNNNNNNNGC 7 cut(s) 22, 578, 839, 1091, 1325, 1455, 1955
BstNI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
BstNSI RCATGY 1 cut(s) 657
BstPAI GACNNNNGTC 1 cut(s) 1004
BstPI GGTNACC 1 cut(s) 917
BstSCI CCNGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
BstSFI CTRYAG 2 cut(s) 249, 956
BstUI CGCG 1 cut(s) 1328
BstV1I GCAGC 8 cut(s) 9, 520, 538, 755, 758, 842, 939, 1318
BstV2I GAAGAC 2 cut(s) 1251, 1252
BstX2I RGATCY 2 cut(s) 1778, 1784
BstYI RGATCY 2 cut(s) 1778, 1784
BsuI GTATCC 2 cut(s) 665, 1173
BsuRI GGCC 3 cut(s) 5, 911, 978
BtgI CCRYGG 1 cut(s) 1821
BtgZI GCGATG 2 cut(s) 672, 1915
BtsCI GGATG 6 cut(s) 274, 764, 871, 1403, 1556, 1840
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 4 cut(s) 270, 682, 1375, 1424
BveI ACCTGC 1 cut(s) 1523
Cac8I GCNNGC 6 cut(s) 261, 319, 531, 616, 828, 1348
CaiI CAGNNNCTG 2 cut(s) 92, 353
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 2 cut(s) 4, 1030
Csp6I GTAC 1 cut(s) 737
CviAII CATG 4 cut(s) 76, 623, 654, 1822
CviQI GTAC 1 cut(s) 737
DdeI CTNAG 7 cut(s) 240, 582, 640, 849, 1047, 1208, 1315
DpnI GATC 5 cut(s) 96, 954, 1780, 1786, 1818
DpnII GATC 5 cut(s) 94, 952, 1778, 1784, 1816
DraI TTTAAA 1 cut(s) 1995
Eam1104I CTCTTC 2 cut(s) 181, 545
EarI CTCTTC 2 cut(s) 181, 545
Eco130I CCWWGG 3 cut(s) 787, 922, 1821
Eco147I AGGCCT 1 cut(s) 911
Eco31I GGTCTC 1 cut(s) 1877
Eco32I GATATC 2 cut(s) 131, 807
Eco47I GGWCC 1 cut(s) 1030
Eco91I GGTNACC 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 1030
EcoO65I GGTNACC 1 cut(s) 917
EcoRII CCWGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
EcoRV GATATC 2 cut(s) 131, 807
EcoT14I CCWWGG 3 cut(s) 787, 922, 1821
ErhI CCWWGG 3 cut(s) 787, 922, 1821
Esp3I CGTCTC 2 cut(s) 992, 1735
FaeI CATG 4 cut(s) 79, 626, 657, 1825
FalI AAGNNNNNCTT 2 cut(s) 1040, 1072
FaqI GGGAC 2 cut(s) 1087, 1839
FatI CATG 4 cut(s) 75, 622, 653, 1821
FauNDI CATATG 1 cut(s) 1708
Fnu4HI GCNGC 9 cut(s) 23, 527, 534, 744, 747, 831, 928, 1329, 1332
FokI GGATG 6 cut(s) 281, 751, 878, 1390, 1543, 1847
Fsp4HI GCNGC 9 cut(s) 23, 527, 534, 744, 747, 831, 928, 1329, 1332
FspBI CTAG 3 cut(s) 72, 318, 1100
GluI GCNGC 9 cut(s) 23, 527, 534, 744, 747, 831, 928, 1329, 1332
GsaI CCCAGC 2 cut(s) 1074, 1524
GsuI CTGGAG 2 cut(s) 83, 369
HaeIII GGCC 3 cut(s) 5, 911, 978
HapII CCGG 2 cut(s) 63, 903
Hin1II CATG 4 cut(s) 79, 626, 657, 1825
HindIII AAGCTT 1 cut(s) 280
HpaII CCGG 2 cut(s) 63, 903
HphI GGTGA 2 cut(s) 892, 1277
Hpy188I TCNGA 9 cut(s) 215, 349, 631, 964, 1138, 1209, 1697, 1816, 1877
Hpy188III TCNNGA 8 cut(s) 63, 92, 100, 623, 776, 810, 1633, 1782
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 6 cut(s) 187, 368, 668, 1021, 1563, 1630
HpyCH4IV ACGT 2 cut(s) 311, 476
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 578, 839, 1091, 1325, 1455, 1955
HpyF3I CTNAG 7 cut(s) 240, 582, 640, 849, 1047, 1208, 1315
HpySE526I ACGT 2 cut(s) 311, 476
Hsp92II CATG 4 cut(s) 79, 626, 657, 1825
Kpn2I TCCGGA 1 cut(s) 62
Kzo9I GATC 5 cut(s) 94, 952, 1778, 1784, 1816
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 4 cut(s) 208, 421, 1697, 1926
Lsp1109I GCAGC 8 cut(s) 9, 520, 538, 755, 758, 842, 939, 1318
MaeI CTAG 3 cut(s) 72, 318, 1100
MaeII ACGT 2 cut(s) 311, 476
MaeIII GTNAC 3 cut(s) 917, 1283, 1853
MalI GATC 5 cut(s) 96, 954, 1780, 1786, 1818
MboI GATC 5 cut(s) 94, 952, 1778, 1784, 1816
MboII GAAGA 8 cut(s) 198, 562, 1048, 1182, 1252, 1256, 1445, 1890
MfeI CAATTG 3 cut(s) 450, 1320, 1866
MflI RGATCY 2 cut(s) 1778, 1784
MlyI GAGTC 5 cut(s) 92, 791, 1211, 1728, 1887
MmeI TCCRAC 1 cut(s) 1812
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 1055
MseI TTAA 6 cut(s) 539, 872, 981, 1400, 1982, 1994
MslI CAYNNNNRTG 2 cut(s) 1290, 1412
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 2 cut(s) 63, 903
MspR9I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
MunI CAATTG 3 cut(s) 450, 1320, 1866
MvaI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
MvnI CGCG 1 cut(s) 1328
MwoI GCNNNNNNNGC 7 cut(s) 22, 578, 839, 1091, 1325, 1455, 1955
NcoI CCATGG 1 cut(s) 1821
NdeI CATATG 1 cut(s) 1708
NdeII GATC 5 cut(s) 94, 952, 1778, 1784, 1816
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 4 cut(s) 79, 626, 657, 1825
NlaIV GGNNCC 4 cut(s) 6, 446, 1018, 1786
NmuCI GTSAC 1 cut(s) 1283
NspI RCATGY 1 cut(s) 657
NspV TTCGAA 1 cut(s) 176
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 911
PciSI GCTCTTC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 7 cut(s) 178, 344, 378, 626, 632, 758, 1124
PfoI TCCNGGA 2 cut(s) 181, 1787
PkrI GCNGC 9 cut(s) 24, 528, 535, 745, 748, 832, 929, 1330, 1333
PleI GAGTC 5 cut(s) 91, 791, 1211, 1727, 1886
PpsI GAGTC 5 cut(s) 91, 791, 1211, 1727, 1886
Ppu21I YACGTR 1 cut(s) 477
PpuMI RGGWCCY 1 cut(s) 1030
PshAI GACNNNNGTC 1 cut(s) 1004
Psp5II RGGWCCY 1 cut(s) 1030
Psp6I CCWGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 2 cut(s) 1070, 1520
PspGI CCWGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
PspN4I GGNNCC 4 cut(s) 6, 446, 1018, 1786
PspPI GGNCC 2 cut(s) 4, 1030
PspPPI RGGWCCY 1 cut(s) 1030
PstI CTGCAG 2 cut(s) 253, 960
PstNI CAGNNNCTG 2 cut(s) 92, 353
PsuI RGATCY 2 cut(s) 1778, 1784
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 1 cut(s) 738
RsaNI GTAC 1 cut(s) 737
RseI CAYNNNNRTG 2 cut(s) 1290, 1412
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 6 cut(s) 539, 872, 981, 1400, 1982, 1994
SatI GCNGC 9 cut(s) 23, 527, 534, 744, 747, 831, 928, 1329, 1332
Sau3AI GATC 5 cut(s) 94, 952, 1778, 1784, 1816
Sau96I GGNCC 2 cut(s) 4, 1030
ScaI AGTACT 1 cut(s) 738
SchI GAGTC 5 cut(s) 92, 791, 1211, 1728, 1887
ScrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1789
SfcI CTRYAG 2 cut(s) 249, 956
SfuI TTCGAA 1 cut(s) 176
SinI GGWCC 1 cut(s) 1030
SmiMI CAYNNNNRTG 2 cut(s) 1290, 1412
SseBI AGGCCT 1 cut(s) 911
SsiI CCGC 1 cut(s) 1328
SspI AATATT 3 cut(s) 297, 706, 1912
SspMI CTAG 3 cut(s) 72, 318, 1100
StuI AGGCCT 1 cut(s) 911
StyD4I CCNGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1787
StyI CCWWGG 3 cut(s) 787, 922, 1821
TaiI ACGT 2 cut(s) 314, 479
TaqI TCGA 6 cut(s) 142, 176, 951, 1008, 1634, 1905
TatI WGTACW 1 cut(s) 736
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 7 cut(s) 178, 344, 378, 626, 632, 758, 1124
Tru1I TTAA 6 cut(s) 539, 872, 981, 1400, 1982, 1994
Tru9I TTAA 6 cut(s) 539, 872, 981, 1400, 1982, 1994
TscAI CASTG 4 cut(s) 270, 682, 1375, 1431
TseFI GTSAC 1 cut(s) 1283
TseI GCWGC 8 cut(s) 22, 526, 533, 743, 746, 830, 927, 1331
Tsp45I GTSAC 1 cut(s) 1283
TspDTI ATGAA 9 cut(s) 38, 285, 453, 623, 699, 810, 1052, 1545, 1563
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 4 cut(s) 270, 682, 1375, 1431
VpaK11BI GGWCC 1 cut(s) 1030
XapI RAATTY 3 cut(s) 409, 1772, 1894
XceI RCATGY 1 cut(s) 657
XcmI CCANNNNNNNNNTGG 2 cut(s) 1828, 1942
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 3 cut(s) 72, 318, 1100
ZrmI AGTACT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.