Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012086

Physical Location & Seq

Genomic Coordinates Reverse (-)

32737045 .. 32747535

Gene Structure 10491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1267200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGGTCATGAAATTCCTTCAACTGCTTTTCTTGCTCAGCTTTCAGCTTCGTCTCTCGTTCTCTGGACATACAAGCAAGATGGAGTCAGTTCCTGATCTTCAGAGTTCAATGTATATGACACTTGATGGATATCCTTGTGTACGGCTACTCAATCTTTCTGGGGAGATTGGTTGTTCAAATCCTGGAAGAGAGAAGGTTGTCGCTCCGATTATAAGATTGACCAATGCTACTGAGTTGTCTCAGTTATCTGCAGTTCTGTTGTCAGTGGATGAAATTCAAAGTTTTCTTGCCAGAATGTTGAATGATTCAAATTTTGCTAAAAATGTTGCTGGTGTCTTGGTTGAATCCAGACCTGAGTTGCAAAATCAGTTGAAAGGATTCTCTCCAGCTCAAAAGTTCCCACAATCTGAATTTGCTCCTTACACTAGCATCAACTATGAATGGAACCCAACTGGCTCTGGTATAATGTGGAACCCATATAACTTTCCTGTATTCTTACTCTCGCAGACTAGCACATTGACCATGCAGGAGGCTGCCATAAAGAATCAGAAGAGCAAGAAATCTTATACTGATGATGTGGCTGAGTTTGATCTGGTAATGCAGACAACGAAATCTGGGACTCATGATTCCGAATCTTGTCTAAAGGAAAACACTTGCCTTCCCTTAGGTGGATACAGTGTTTGGTCATCACTTCCCCCAATCAACATTTCATCACCAGAACACTCCAAGCCCATTATACTTGTACTGACGTCAATGGATTCAGCTTCTTTTTTCCGTGATAAAGCACTCGGTGCCGACTCTCCTATATCTGGGCTGATTTCATTGCTGGCCGCAGTTGATGCACTTTCTCGTGCGGATGATCTGGATGGCTTCAATAAACAGCTTGTTTTCGCTGTTTTCACTGGAGAGGCCTGGGGCTATCTTGGTAGCAGGAGATTTTTGCTTGAACTTGATCTGCAGTCTAATGCTGTTAGTGGTCTTAATTACTCATTGATTGAGAAGGTTGTGGAAATTGGATCTGTCGGAAAGGGCCTGAACCAAGGAGCTAAGAACTTTTTCATTCATACAACTGGGGTTTCATCTTCCACAAATGAGACATTGGACGCTGTGAGGCGTGCCCAAGATTCTGTCAAGTCTGAAAACATTACGATCTCGTCTGCAAATGCTTCAAATCCTGGGATACCTCCATCCTCCTTGATGACTTTTCTGAGAAAGAACTCTCTAACCTCTGGGGTTGTGTTAGAAGATTTTGATAATGTCTTCACCAACAAGTTCTACCACAGTCACCTTGATGATTTATCAAATATCAACTCATCATCCATTGCGGCAGCTGCTTCCCTTGTAGCACGTGCCCTTTACATTCTTGCAAGTGACACCAAAAATTTAAGCAGTTCTGCTCTGACGTCTATCAACGTGAATGCTTCACTAGTTGAAGAACTAGTGGGTTGCCTGGTGGACTGTGAGCCAGGTCTTTCTTGTGATCTAGTGAAAAGCTATATATCACCAGCCAATACCTGCCCGAGCCATTATGTTGGCGTTATCCTTGATGAACCTTCCTCCACACCTGATCTGGGATACGTTGGTGACATTTCAAGGTTCGTTTGGAACTTCCTGGCCGAAAGAACTTCTATTCCGAAGGAAAATGCGAGCTCTGTTTGTACTCAAGACTGCAGTAATGATGGTGAAGTATGTATTAGAGCAGAAACAGAGGGAAAGGGTGTCTGTGTTGTCTCCACAACCAGATATGTTCCGGCATACTCAACACGGTTGAAATTTGAATCTGGTGGAACGTGGACTGTGTTGCCTCCGAATAACTCTGACCCCATGGGGCTGTTGGACGCTGTTTGGACAGAGAGCAACTGGGATGTGATCGGGCTTCGGGTCTACACTGTCCAAAATTCTTCTTTCGATCGTCTCGTCTTGCTCGGGAGCATTGCTGTTACAGTCTTGGCTTACCTTGCAATAGTGATTTTCAAGGGCTGCGTGACGAAGGCTTTAAAACGCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0010467 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0031226 GO:0043170 GO:0044238 GO:0044425 GO:0044459 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04330 ko05010 map04330 map05010

Pfam Domains

Protein Families

Nicastrin

Protein Analysis

670

Amino Acids

72.6

Weight (kDa)

5.05

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ncstrn_small	PF18266	45 - 202	5.3e-43	Nicastrin small lobe
Nicastrin	PF05450	244 - 453	8.6e-74	Nicastrin large lobe

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012086)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52640 AT3G52640 AT3G52640
fragaria_vesca	FvH4_1g30060
malus_domestica	MD17G1267200.v1.1
prunus_persica	Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis	pycom17g26630
rosa_chinensis	RchiOBHm_Chr3g0492061
rosa_laevigata	RLG00000022797
rosa_multiflora	Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392600
rosa_rugosa	Rorug03G0251500
rosa_samantha	Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana	Rw3G026630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	212
AatII	GACGTC	2 cut(s)	752, 1406
Acc36I	ACCTGC	1 cut(s)	1523
AccB1I	GGYRCC	1 cut(s)	791
AccI	GTMKAC	1 cut(s)	1884
AccII	CGCG	1 cut(s)	2004
AciI	CCGC	3 cut(s)	831, 854, 1325
AclWI	GGATC	1 cut(s)	1024
AcoI	YGGCCR	2 cut(s)	828, 1614
AcsI	RAATTY	7 cut(s)	11, 273, 310, 410, 1381, 1772, 1897
AcuI	CTGAAG	1 cut(s)	83
AcvI	CACGTG	1 cut(s)	1349
AcyI	GRCGYC	2 cut(s)	749, 1403
AdeI	CACNNNGTG	1 cut(s)	791
AfaI	GTAC	3 cut(s)	141, 744, 1660
AfiI	CCNNNNNNNGG	3 cut(s)	668, 809, 1571
AhlI	ACTAGT	2 cut(s)	1426, 1438
AjnI	CCWGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
AjuI	GAANNNNNNNTTGG	2 cut(s)	691, 723
AloI	GAACNNNNNNTCC	2 cut(s)	1615, 1647
AluBI	AGCT	9 cut(s)	39, 46, 389, 764, 883, 1046, 1331, 1494, 1650
AluI	AGCT	9 cut(s)	39, 46, 389, 764, 883, 1046, 1331, 1494, 1650
Alw21I	GWGCWC	1 cut(s)	1652
Alw26I	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
AlwI	GGATC	1 cut(s)	1024
AlwNI	CAGNNNCTG	1 cut(s)	92
Ama87I	CYCGRG	2 cut(s)	1519, 1925
AoxI	GGCC	4 cut(s)	828, 909, 1030, 1614
ApeKI	GCWGC	4 cut(s)	533, 1328, 1331, 1980
ApoI	RAATTY	7 cut(s)	11, 273, 310, 410, 1381, 1772, 1897
Asp700I	GAANNNNTTC	2 cut(s)	377, 1055
AspS9I	GGNCC	1 cut(s)	1030
AsuHPI	GGTGA	6 cut(s)	705, 1255, 1277, 1494, 1595, 1694
AvaI	CYCGRG	2 cut(s)	1519, 1925
AxyI	CCTNAGG	1 cut(s)	664
BaeGI	GKGCMC	2 cut(s)	1120, 1354
BaeI	ACNNNNGTAYC	2 cut(s)	1567, 1600
BanI	GGYRCC	1 cut(s)	791
BanII	GRGCYC	1 cut(s)	1652
BauI	CACGAG	1 cut(s)	849
BbrPI	CACGTG	1 cut(s)	1349
BbsI	GAAGAC	1 cut(s)	1252
Bbv12I	GWGCWC	1 cut(s)	1652
BbvI	GCAGC	4 cut(s)	520, 1318, 1340, 1967
BccI	CCATC	5 cut(s)	73, 119, 860, 1195, 1673
BceAI	ACGGC	1 cut(s)	158
BciT130I	CCWGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
BciVI	GTATCC	3 cut(s)	665, 1173, 1568
BcoDI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BcuI	ACTAGT	2 cut(s)	1426, 1438
BfaI	CTAG	5 cut(s)	426, 510, 1427, 1439, 1484
BfmI	CTRYAG	3 cut(s)	249, 956, 1669
BfuAI	ACCTGC	1 cut(s)	1523
BfuI	GTATCC	3 cut(s)	665, 1173, 1568
BisI	GCNGC	6 cut(s)	534, 831, 1326, 1329, 1332, 1981
BlpI	GCTNAGC	1 cut(s)	35
BlsI	GCNGC	6 cut(s)	535, 832, 1327, 1330, 1333, 1982
Bme1390I	CCNGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
BmeT110I	CYCGRG	2 cut(s)	1519, 1925
BmgT120I	GGNCC	1 cut(s)	1030
BmiI	GGNNCC	3 cut(s)	446, 473, 793
BmrFI	CCNGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
BmrI	ACTGGG	2 cut(s)	1080, 1870
BmsI	GCATC	2 cut(s)	438, 829
BmuI	ACTGGG	2 cut(s)	1080, 1870
BpiI	GAAGAC	1 cut(s)	1252
BpmI	CTGGAG	2 cut(s)	369, 924
Bpu1102I	GCTNAGC	1 cut(s)	35
BpuEI	CTTGAG	1 cut(s)	1647
BsaAI	YACGTR	1 cut(s)	1349
BsaBI	GATNNNNATC	1 cut(s)	129
BsaHI	GRCGYC	2 cut(s)	749, 1403
BsaJI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
Bsc4I	CCNNNNNNNGG	3 cut(s)	668, 809, 1571
Bse1I	ACTGG	4 cut(s)	457, 907, 1075, 1865
Bse21I	CCTNAGG	1 cut(s)	664
Bse3DI	GCAATG	3 cut(s)	821, 1320, 1932
Bse8I	GATNNNNATC	1 cut(s)	129
BseBI	CCWGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
BseDI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
BseGI	GGATG	6 cut(s)	274, 862, 871, 1187, 1316, 1870
BseJI	GATNNNNATC	1 cut(s)	129
BseLI	CCNNNNNNNGG	3 cut(s)	668, 809, 1571
BseMI	GCAATG	3 cut(s)	821, 1320, 1932
BseMII	CTCAG	6 cut(s)	49, 222, 254, 345, 573, 1199
BseNI	ACTGG	4 cut(s)	457, 907, 1075, 1865
BseSI	GKGCMC	2 cut(s)	1120, 1354
BseXI	GCAGC	4 cut(s)	520, 1318, 1340, 1967
Bsh1236I	CGCG	1 cut(s)	2004
Bsh1285I	CGRYCG	1 cut(s)	1912
BshFI	GGCC	4 cut(s)	830, 911, 1032, 1616
BshNI	GGYRCC	1 cut(s)	791
BsiEI	CGRYCG	1 cut(s)	1912
BsiHKAI	GWGCWC	1 cut(s)	1652
BsiHKCI	CYCGRG	2 cut(s)	1519, 1925
BsiSI	CCGG	1 cut(s)	1751
BslFI	GGGAC	1 cut(s)	631
BslI	CCNNNNNNNGG	3 cut(s)	668, 809, 1571
BsmAI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BsmBI	CGTCTC	2 cut(s)	56, 1919
BsmFI	GGGAC	1 cut(s)	631
BsmI	GAATGC	1 cut(s)	1423
BsnI	GGCC	4 cut(s)	830, 911, 1032, 1616
BsoBI	CYCGRG	2 cut(s)	1519, 1925
Bsp1286I	GDGCHC	3 cut(s)	1120, 1354, 1652
Bsp1720I	GCTNAGC	1 cut(s)	35
Bsp19I	CCATGG	1 cut(s)	1824
BspACI	CCGC	3 cut(s)	831, 854, 1325
BspANI	GGCC	4 cut(s)	830, 911, 1032, 1616
BspCNI	CTCAG	6 cut(s)	48, 223, 253, 346, 574, 1200
BspFNI	CGCG	1 cut(s)	2004
BspHI	TCATGA	2 cut(s)	6, 622
BspLI	GGNNCC	3 cut(s)	446, 473, 793
BspMAI	CTGCAG	3 cut(s)	253, 960, 1673
BspMI	ACCTGC	1 cut(s)	1523
BspPI	GGATC	1 cut(s)	1024
BspQI	GCTCTTC	1 cut(s)	545
BspT107I	GGYRCC	1 cut(s)	791
BsrDI	GCAATG	3 cut(s)	821, 1320, 1932
BsrI	ACTGG	4 cut(s)	457, 907, 1075, 1865
BssECI	CCNNGG	4 cut(s)	912, 1039, 1175, 1824
BssNI	GRCGYC	2 cut(s)	749, 1403
BssSI	CACGAG	1 cut(s)	849
BssT1I	CCWWGG	2 cut(s)	1039, 1824
Bst2BI	CACGAG	1 cut(s)	849
Bst2UI	CCWGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
Bst4CI	ACNGT	7 cut(s)	677, 1283, 1460, 1767, 1798, 1891, 1945
Bst6I	CTCTTC	2 cut(s)	181, 545
BstACI	GRCGYC	2 cut(s)	749, 1403
BstAPI	GCANNNNNTGC	2 cut(s)	791, 839
BstBAI	YACGTR	1 cut(s)	1349
BstC8I	GCNNGC	3 cut(s)	828, 1116, 1648
BstDEI	CTNAG	8 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208
BstDSI	CCRYGG	1 cut(s)	1824
BstF5I	GGATG	6 cut(s)	274, 862, 871, 1187, 1316, 1870
BstFNI	CGCG	1 cut(s)	2004
BstMAI	GTCTC	5 cut(s)	56, 243, 1088, 1735, 1919
BstMCI	CGRYCG	1 cut(s)	1912
BstMWI	GCNNNNNNNGC	5 cut(s)	31, 791, 839, 1331, 1958
BstNI	CCWGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
BstSCI	CCNGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
BstSFI	CTRYAG	3 cut(s)	249, 956, 1669
BstSLI	GKGCMC	2 cut(s)	1120, 1354
BstUI	CGCG	1 cut(s)	2004
BstV1I	GCAGC	4 cut(s)	520, 1318, 1340, 1967
BstV2I	GAAGAC	1 cut(s)	1252
BstX2I	RGATCY	1 cut(s)	1016
BstXI	CCANNNNNNTGG	1 cut(s)	1532
BstYI	RGATCY	1 cut(s)	1016
Bsu36I	CCTNAGG	1 cut(s)	664
BsuI	GTATCC	3 cut(s)	665, 1173, 1568
BsuRI	GGCC	4 cut(s)	830, 911, 1032, 1616
BtgI	CCRYGG	1 cut(s)	1824
BtsCI	GGATG	6 cut(s)	274, 862, 871, 1187, 1316, 1870
BtsIMutI	CAGTG	4 cut(s)	270, 682, 900, 1887
BveI	ACCTGC	1 cut(s)	1523
Cac8I	GCNNGC	3 cut(s)	828, 1116, 1648
CaiI	CAGNNNCTG	1 cut(s)	92
CciI	TCATGA	2 cut(s)	6, 622
Cfr13I	GGNCC	1 cut(s)	1030
CseI	GACGC	2 cut(s)	1112, 1847
Csp6I	GTAC	3 cut(s)	140, 743, 1659
CviAII	CATG	4 cut(s)	7, 523, 623, 1825
CviQI	GTAC	3 cut(s)	140, 743, 1659
DdeI	CTNAG	8 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208
DraI	TTTAAA	1 cut(s)	1998
DraIII	CACNNNGTG	1 cut(s)	791
EaeI	YGGCCR	2 cut(s)	828, 1614
Eam1104I	CTCTTC	2 cut(s)	181, 545
EarI	CTCTTC	2 cut(s)	181, 545
Ecl136II	GAGCTC	1 cut(s)	1650
Eco130I	CCWWGG	2 cut(s)	1039, 1824
Eco147I	AGGCCT	1 cut(s)	911
Eco24I	GRGCYC	1 cut(s)	1652
Eco32I	GATATC	1 cut(s)	131
Eco53kI	GAGCTC	1 cut(s)	1650
Eco57I	CTGAAG	1 cut(s)	83
Eco72I	CACGTG	1 cut(s)	1349
Eco81I	CCTNAGG	1 cut(s)	664
Eco88I	CYCGRG	2 cut(s)	1519, 1925
EcoICRI	GAGCTC	1 cut(s)	1650
EcoO109I	RGGNCCY	1 cut(s)	1030
EcoRII	CCWGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
EcoRV	GATATC	1 cut(s)	131
EcoT14I	CCWWGG	2 cut(s)	1039, 1824
EcoT38I	GRGCYC	1 cut(s)	1652
ErhI	CCWWGG	2 cut(s)	1039, 1824
Esp3I	CGTCTC	2 cut(s)	56, 1919
FaeI	CATG	4 cut(s)	10, 526, 626, 1828
FaqI	GGGAC	1 cut(s)	631
FatI	CATG	4 cut(s)	6, 522, 622, 1824
FblI	GTMKAC	1 cut(s)	1884
Fnu4HI	GCNGC	6 cut(s)	534, 831, 1326, 1329, 1332, 1981
FokI	GGATG	6 cut(s)	281, 869, 878, 1174, 1303, 1877
FriOI	GRGCYC	1 cut(s)	1652
Fsp4HI	GCNGC	6 cut(s)	534, 831, 1326, 1329, 1332, 1981
FspBI	CTAG	5 cut(s)	426, 510, 1427, 1439, 1484
GluI	GCNGC	6 cut(s)	534, 831, 1326, 1329, 1332, 1981
GsuI	CTGGAG	2 cut(s)	369, 924
HaeIII	GGCC	4 cut(s)	830, 911, 1032, 1616
HapII	CCGG	1 cut(s)	1751
HgaI	GACGC	2 cut(s)	1112, 1847
Hin1I	GRCGYC	2 cut(s)	749, 1403
Hin1II	CATG	4 cut(s)	10, 526, 626, 1828
HpaII	CCGG	1 cut(s)	1751
HphI	GGTGA	6 cut(s)	705, 1255, 1277, 1494, 1595, 1694
Hpy166II	GTNNAC	4 cut(s)	140, 1456, 1794, 1885
Hpy188III	TCNNGA	8 cut(s)	7, 63, 92, 348, 623, 863, 1664, 1927
Hpy8I	GTNNAC	4 cut(s)	140, 1456, 1794, 1885
HpyAV	CCTTC	7 cut(s)	26, 187, 668, 994, 1563, 1630, 1984
HpyCH4III	ACNGT	7 cut(s)	677, 1283, 1460, 1767, 1798, 1891, 1945
HpyCH4IV	ACGT	6 cut(s)	749, 1348, 1403, 1413, 1578, 1790
HpyF10VI	GCNNNNNNNGC	5 cut(s)	31, 791, 839, 1331, 1958
HpyF3I	CTNAG	8 cut(s)	35, 231, 240, 354, 582, 664, 1047, 1208
HpySE526I	ACGT	6 cut(s)	749, 1348, 1403, 1413, 1578, 1790
Hsp92I	GRCGYC	2 cut(s)	749, 1403
Hsp92II	CATG	4 cut(s)	10, 526, 626, 1828
LguI	GCTCTTC	1 cut(s)	545
LmnI	GCTCC	4 cut(s)	208, 421, 1043, 1929
Lsp1109I	GCAGC	4 cut(s)	520, 1318, 1340, 1967
LweI	GCATC	2 cut(s)	438, 829
MaeI	CTAG	5 cut(s)	426, 510, 1427, 1439, 1484
MaeII	ACGT	6 cut(s)	749, 1348, 1403, 1413, 1578, 1790
MaeIII	GTNAC	5 cut(s)	1283, 1370, 1583, 1939, 1984
MboII	GAAGA	8 cut(s)	89, 198, 562, 1074, 1252, 1256, 1445, 1893
MflI	RGATCY	1 cut(s)	1016
MhlI	GDGCHC	3 cut(s)	1120, 1354, 1652
MluCI	AATT	9 cut(s)	11, 273, 310, 410, 982, 1011, 1381, 1772, 1897
MlyI	GAGTC	3 cut(s)	92, 613, 791
MmeI	TCCRAC	2 cut(s)	1003, 1815
MnlI	CCTC	9 cut(s)	523, 901, 1104, 1194, 1201, 1237, 1567, 1702, 1815
MroXI	GAANNNNTTC	2 cut(s)	377, 1055
MseI	TTAA	3 cut(s)	981, 1385, 1997
MslI	CAYNNNNRTG	1 cut(s)	1290
MspA1I	CMGCKG	1 cut(s)	1331
MspI	CCGG	1 cut(s)	1751
MspR9I	CCNGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
Mva1269I	GAATGC	1 cut(s)	1423
MvaI	CCWGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
MvnI	CGCG	1 cut(s)	2004
MwoI	GCNNNNNNNGC	5 cut(s)	31, 791, 839, 1331, 1958
NcoI	CCATGG	1 cut(s)	1824
NlaIII	CATG	4 cut(s)	10, 526, 626, 1828
NlaIV	GGNNCC	3 cut(s)	446, 473, 793
NmuCI	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
PagI	TCATGA	2 cut(s)	6, 622
PceI	AGGCCT	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	545
PcsI	WCGNNNNNNNCGW	1 cut(s)	1914
PctI	GAATGC	1 cut(s)	1423
PdmI	GAANNNNTTC	2 cut(s)	377, 1055
PfeI	GAWTC	9 cut(s)	305, 344, 378, 544, 626, 632, 758, 1124, 1778
PfoI	TCCNGGA	1 cut(s)	181
PkrI	GCNGC	6 cut(s)	535, 832, 1327, 1330, 1333, 1982
Ple19I	CGATCG	1 cut(s)	1912
PleI	GAGTC	3 cut(s)	91, 613, 791
PmaCI	CACGTG	1 cut(s)	1349
PmlI	CACGTG	1 cut(s)	1349
PpsI	GAGTC	3 cut(s)	91, 613, 791
Ppu21I	YACGTR	1 cut(s)	1349
PsiI	TTATAA	1 cut(s)	212
Psp124BI	GAGCTC	1 cut(s)	1652
Psp6I	CCWGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
PspCI	CACGTG	1 cut(s)	1349
PspGI	CCWGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
PspN4I	GGNNCC	3 cut(s)	446, 473, 793
PspPI	GGNCC	1 cut(s)	1030
PstI	CTGCAG	3 cut(s)	253, 960, 1673
PstNI	CAGNNNCTG	1 cut(s)	92
PsuI	RGATCY	1 cut(s)	1016
PvuI	CGATCG	1 cut(s)	1912
PvuII	CAGCTG	1 cut(s)	1331
RsaI	GTAC	3 cut(s)	141, 744, 1660
RsaNI	GTAC	3 cut(s)	140, 743, 1659
RseI	CAYNNNNRTG	1 cut(s)	1290
SacI	GAGCTC	1 cut(s)	1652
SapI	GCTCTTC	1 cut(s)	545
SaqAI	TTAA	3 cut(s)	981, 1385, 1997
SatI	GCNGC	6 cut(s)	534, 831, 1326, 1329, 1332, 1981
Sau96I	GGNCC	1 cut(s)	1030
SchI	GAGTC	3 cut(s)	92, 613, 791
ScrFI	CCNGG	6 cut(s)	183, 913, 1176, 1451, 1467, 1613
SduI	GDGCHC	3 cut(s)	1120, 1354, 1652
SfaNI	GCATC	2 cut(s)	438, 829
SfcI	CTRYAG	3 cut(s)	249, 956, 1669
SmiMI	CAYNNNNRTG	1 cut(s)	1290
SmlI	CTYRAG	1 cut(s)	1662
SmoI	CTYRAG	1 cut(s)	1662
SpeI	ACTAGT	2 cut(s)	1426, 1438
Sse9I	AATT	9 cut(s)	11, 273, 310, 410, 982, 1011, 1381, 1772, 1897
SseBI	AGGCCT	1 cut(s)	911
SsiI	CCGC	3 cut(s)	831, 854, 1325
SspMI	CTAG	5 cut(s)	426, 510, 1427, 1439, 1484
SstI	GAGCTC	1 cut(s)	1652
StuI	AGGCCT	1 cut(s)	911
StyD4I	CCNGG	6 cut(s)	181, 911, 1174, 1449, 1465, 1611
StyI	CCWWGG	2 cut(s)	1039, 1824
TaaI	ACNGT	7 cut(s)	677, 1283, 1460, 1767, 1798, 1891, 1945
TaiI	ACGT	6 cut(s)	752, 1351, 1406, 1416, 1581, 1793
TaqI	TCGA	1 cut(s)	1908
TasI	AATT	9 cut(s)	11, 273, 310, 410, 982, 1011, 1381, 1772, 1897
TatI	WGTACW	2 cut(s)	742, 1658
TauI	GCSGC	2 cut(s)	833, 1328
TfiI	GAWTC	9 cut(s)	305, 344, 378, 544, 626, 632, 758, 1124, 1778
Tru1I	TTAA	3 cut(s)	981, 1385, 1997
Tru9I	TTAA	3 cut(s)	981, 1385, 1997
TscAI	CASTG	4 cut(s)	270, 682, 907, 1894
TseFI	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
TseI	GCWGC	4 cut(s)	533, 1328, 1331, 1980
Tsp45I	GTSAC	4 cut(s)	1283, 1370, 1583, 1984
TspDTI	ATGAA	9 cut(s)	23, 285, 453, 699, 810, 1048, 1052, 1068, 1563
TspGWI	ACGGA	1 cut(s)	764
TspRI	CASTG	4 cut(s)	270, 682, 907, 1894
XapI	RAATTY	7 cut(s)	11, 273, 310, 410, 1381, 1772, 1897
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1567, 1831
XmiI	GTMKAC	1 cut(s)	1884
XmnI	GAANNNNTTC	2 cut(s)	377, 1055
XspI	CTAG	5 cut(s)	426, 510, 1427, 1439, 1484
ZraI	GACGTC	2 cut(s)	750, 1404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.