Rw3G026630

Nicastrin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0012086
Physical Location & Seq
Reverse (-)
36684431 .. 36696232
11802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026630.1

Sequence Viewer

Length: 2004 bp
ATGGCCGCCAAATTCCTTCAACTGCTTTTGCTCTTGAGTTTTCAGCTTCATCTATCTTTCTCCGGACAGACTAGCATGATGGAGTCAGTTCCTGATCTCCAGAGTTCCATGTACATGACGATTGATGGATATCCTTGTGTTCGACTACTCAATCTTTCTGGGGAGATTGGTTGTTCAAATCCTGGAAGAGAGAAGGTTGTTGCTCCAATTATAAGACTAAATAATGCTAATGAGCTCACTCAGTTGTCTGCAGTTCTGCTTTCAGTGGATGAAATTGAAAGTTTTTTTGCCAGAATATCGAAGGATACGACCTTTGCTAGCAATGTAGCTGGTGTCTTGGTAGAATCAGGGCCTGAGATTCAAAATAAGTTAAAAGGGTTCTCTCCAGCTCTGAAGTTCCCACAATCAGATTTTGCTCCTTACAGAAGCATCAACTACGAATGGAACCCGATTGGATCTGGTATAATGTGGAACACGTATAACTTTCCTGTATTCTTACTCTCCCAGAGTAGCACATTGACCCTGCAGGAGGCTGCCTTAAACAATGAGAAGAGCAAGAAATCTTATACTGCTGATGTGGCTGAGTTTGATTTAGTGATGCAGACAATGAAAGCTGGCACTCATGATTCAAAATCTTGTTTAGAGGAAAATACTTGCCTTCCTTTAGGTGGATACAGTGTTTGGTCATCGCTTCCCCCGATCAATATTTCATCATCAGAGCAGTCCAAGCCCATACTTGCAGTAGCATCAATGGATTCAGCATCATTTTTTCGTGATAAAAACCTTGGTGCTGACTCCCCAATATCTGGACTGATTTCATTGCTGGCAGCAGTTGATGCACTTTCGAAGGGGAATATTGTGGATGACTTTAATAAACAGCTTGTATTTATTGTTTTCACCGGAGAGGCCTGGGGTTACCTTGGTAGCAGGAGATTTTTGCTGGAACTGGATCTGCAGTCTGATGCTGTTAGTGGCCTTAATTACTCATTGATTGAGAGGGTTGTCGAAATAGGTTCCATTGGGAAGGTCCTGAGCCAAGAAGCAAAGAACTTTTTTGTTCATACAGCTGGGACTTCCTCTGCCACAAATGAGACATTGGATGCTTTGAGGATTGCCCAAGATTCAGTCAAGTCTGACCACATTGTCGTCTTGTCTGCAAATGCTTCAAATCCTGGGATACCTCCATCTTCCTTGATGGCTTTTCTGGGAAAGGGCTCTCTAACCTCTGGGGTTGTATTGGAAGACTTTGATACTGTCTTCACCAACAAGTTCTACCAGAGTCACCTTGATGATCTATCAAATATAAACTCTTCAGCTATTGTGGCAGCTGCTTCCCTTGTAGCACGCACCCTTTACATTCTTGCCAGTGGCAATAACAATACAAGCAGTTCGGCTTTAACGTCTATCAATGTGAATGTTTCACTGGTCAAAGAACTAATGGGTTGCCTATTGGACTGTGAGCCAGGTCTTTCTTGTGAGCTGGTTAAAAATTATATAACACCAGCGAATACCTGCCCCAGCAATTATGTTGGCGTTATCGTTGATGAACCTTCATCCACCCCGTATGTTGGATATGTTAGTGATATTTCAAGGTTTGTTTGGAACTTCCTGGCTGAAAAAACTTCTATTTCAAAGGAAAATGCTACCTCTGTCTGTTCTAAAGATTGCAGCAATGATGGTGGAGTCTGCATTAGAGCGGAGACAGATGGAAAGGGAGTTTGTGTTGTCTCCACAACCAGATACGTTCCAGCTTACTCAACAAGGTTGAAATTTGAATCTGGATCCTGGACTGTATTGCCTCCAAATAACTCTGATCCCATGGGGCTGTTGGATGCTGTTTGGACAGAAAGTAACTGGAATACAATTGGGCTCCGAGTTTATACTGTCCAAAATTCTTCTTTCGATAATATTGTTTTACTTGGGAGCATCGCCATTACAGTCTTGGCTTACCTCGCAATAGTGATCACAAGGGCCTTTTTAACGAAGGCTTTAAAGCGTGACTGA

Protein Analysis

667

Amino Acids

72.02

Weight (kDa)

4.91

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ncstrn_small PF18266 45 - 202 7.2e-44 Nicastrin small lobe
Peptidase_M28 PF04389 239 - 449 4.3e-06 Peptidase family M28
Nicastrin PF05450 245 - 452 4.4e-71 Nicastrin large lobe
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52640 AT3G52640 AT3G52640
fragaria_vesca FvH4_1g30060
malus_domestica MD17G1267200.v1.1
prunus_persica Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis pycom17g26630
rosa_chinensis RchiOBHm_Chr3g0492061
rosa_laevigata RLG00000022797
rosa_multiflora Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii Rroxscaffold_6G00392600
rosa_rugosa Rorug03G0251500
rosa_samantha Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana Rw3G026630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 212
AasI GACNNNNNNGTC 1 cut(s) 1142
Acc36I ACCTGC 1 cut(s) 1520
AccB7I CCANNNNNTGG 1 cut(s) 806
AccBSI CCGCTC 1 cut(s) 1697
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 2 cut(s) 6, 1697
AclWI GGATC 5 cut(s) 463, 957, 1776, 1789, 1808
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 11, 1769, 1891
AcuI CTGAAG 2 cut(s) 413, 1296
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 4 cut(s) 529, 668, 806, 1568
AflIII ACRYGT 1 cut(s) 474
AjnI CCWGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 1085, 1694, 1732
AlwI GGATC 5 cut(s) 463, 957, 1776, 1789, 1808
AlwNI CAGNNNCTG 2 cut(s) 92, 353
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 5 cut(s) 3, 350, 906, 973, 1971
ApeKI GCWGC 5 cut(s) 533, 827, 1325, 1328, 1668
ApoI RAATTY 3 cut(s) 11, 1769, 1891
AspS9I GGNCC 3 cut(s) 350, 1027, 1971
AsuHPI GGTGA 3 cut(s) 889, 1252, 1274
AsuII TTCGAA 1 cut(s) 845
AsuNHI GCTAGC 1 cut(s) 317
AvaII GGWCC 1 cut(s) 1027
BamHI GGATCC 1 cut(s) 1781
BanII GRGCYC 3 cut(s) 237, 1217, 1872
BbsI GAAGAC 2 cut(s) 1248, 1249
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 5 cut(s) 520, 839, 1315, 1337, 1680
BccI CCATC 6 cut(s) 73, 119, 1189, 1192, 1670, 1700
BciT130I CCWGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
BciVI GTATCC 3 cut(s) 298, 665, 1170
BclI TGATCA 1 cut(s) 1962
BcoDI GTCTC 3 cut(s) 1085, 1694, 1732
BfaI CTAG 2 cut(s) 72, 318
BfmI CTRYAG 3 cut(s) 249, 524, 953
BfuAI ACCTGC 1 cut(s) 1520
BfuI GTATCC 3 cut(s) 298, 665, 1170
BisI GCNGC 6 cut(s) 6, 534, 828, 1326, 1329, 1669
BlsI GCNGC 6 cut(s) 7, 535, 829, 1327, 1330, 1670
Bme1390I CCNGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
Bme18I GGWCC 1 cut(s) 1027
BmgT120I GGNCC 3 cut(s) 350, 1027, 1971
BmiI GGNNCC 4 cut(s) 446, 1015, 1783, 1871
BmrFI CCNGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
BmsI GCATC 9 cut(s) 438, 588, 755, 770, 826, 952, 1090, 1822, 1935
BmtI GCTAGC 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 1248, 1249
BpmI CTGGAG 2 cut(s) 83, 369
Bpu10I CCTNAGC 1 cut(s) 1031
Bpu14I TTCGAA 1 cut(s) 845
BpuEI CTTGAG 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 5 cut(s) 784, 909, 919, 1172, 1818
BsaWI WCCGGW 2 cut(s) 62, 899
BsaXI ACNNNNNCTCC 2 cut(s) 1707, 1737
Bsc4I CCNNNNNNNGG 4 cut(s) 529, 668, 806, 1568
Bse1I ACTGG 4 cut(s) 951, 1365, 1428, 1859
Bse3DI GCAATG 3 cut(s) 328, 818, 1678
Bse8I GATNNNNATC 1 cut(s) 129
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
BseDI CCNNGG 5 cut(s) 784, 909, 919, 1172, 1818
BseGI GGATG 5 cut(s) 274, 868, 1105, 1553, 1837
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 4 cut(s) 529, 668, 806, 1568
BseMI GCAATG 3 cut(s) 328, 818, 1678
BseMII CTCAG 4 cut(s) 254, 345, 573, 1022
BseNI ACTGG 4 cut(s) 951, 1365, 1428, 1859
BseXI GCAGC 5 cut(s) 520, 839, 1315, 1337, 1680
BseYI CCCAGC 2 cut(s) 1067, 1517
BshFI GGCC 5 cut(s) 5, 352, 908, 975, 1973
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 2 cut(s) 63, 900
BslFI GGGAC 1 cut(s) 1084
BslI CCNNNNNNNGG 4 cut(s) 529, 668, 806, 1568
BsmAI GTCTC 3 cut(s) 1085, 1694, 1732
BsmFI GGGAC 1 cut(s) 1084
BsnI GGCC 5 cut(s) 5, 352, 908, 975, 1973
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 3 cut(s) 237, 1217, 1872
Bsp13I TCCGGA 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
Bsp19I CCATGG 1 cut(s) 1818
BspACI CCGC 2 cut(s) 6, 1697
BspANI GGCC 5 cut(s) 5, 352, 908, 975, 1973
BspCNI CTCAG 4 cut(s) 253, 346, 574, 1023
BspEI TCCGGA 1 cut(s) 62
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 4 cut(s) 446, 1015, 1783, 1871
BspMAI CTGCAG 3 cut(s) 253, 528, 957
BspMI ACCTGC 1 cut(s) 1520
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 5 cut(s) 463, 957, 1776, 1789, 1808
BspQI GCTCTTC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 1697
BsrDI GCAATG 3 cut(s) 328, 818, 1678
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 4 cut(s) 951, 1365, 1428, 1859
BssECI CCNNGG 5 cut(s) 784, 909, 919, 1172, 1818
BssMI GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
BssT1I CCWWGG 3 cut(s) 784, 919, 1818
Bst2UI CCWGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
Bst4CI ACNGT 6 cut(s) 677, 1255, 1457, 1792, 1885, 1939
Bst6I CTCTTC 3 cut(s) 181, 545, 1315
BstAPI GCANNNNNTGC 1 cut(s) 836
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 845
BstC8I GCNNGC 4 cut(s) 319, 616, 825, 1345
BstDEI CTNAG 4 cut(s) 240, 354, 582, 1031
BstDSI CCRYGG 1 cut(s) 1818
BstEII GGTNACC 1 cut(s) 914
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 5 cut(s) 274, 868, 1105, 1553, 1837
BstKTI GATC 8 cut(s) 97, 458, 702, 952, 1294, 1784, 1816, 1965
BstMAI GTCTC 3 cut(s) 1085, 1694, 1732
BstMBI GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
BstMWI GCNNNNNNNGC 5 cut(s) 578, 727, 836, 1322, 1952
BstNI CCWGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
BstSFI CTRYAG 3 cut(s) 249, 524, 953
BstV1I GCAGC 5 cut(s) 520, 839, 1315, 1337, 1680
BstV2I GAAGAC 2 cut(s) 1248, 1249
BstX2I RGATCY 3 cut(s) 455, 949, 1781
BstYI RGATCY 3 cut(s) 455, 949, 1781
BsuI GTATCC 3 cut(s) 298, 665, 1170
BsuRI GGCC 5 cut(s) 5, 352, 908, 975, 1973
BtgI CCRYGG 1 cut(s) 1818
BtgZI GCGATG 2 cut(s) 672, 1912
BtsCI GGATG 5 cut(s) 274, 868, 1105, 1553, 1837
BtsIMutI CAGTG 4 cut(s) 270, 682, 1372, 1421
BveI ACCTGC 1 cut(s) 1520
Cac8I GCNNGC 4 cut(s) 319, 616, 825, 1345
CaiI CAGNNNCTG 2 cut(s) 92, 353
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 3 cut(s) 350, 1027, 1971
Csp6I GTAC 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 1660, 1695
CviAII CATG 5 cut(s) 76, 109, 115, 623, 1819
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 4 cut(s) 240, 354, 582, 1031
DpnI GATC 8 cut(s) 96, 457, 701, 951, 1293, 1783, 1815, 1964
DpnII GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
DraI TTTAAA 1 cut(s) 1992
DrdI GACNNNNNNGTC 1 cut(s) 1142
DseDI GACNNNNNNGTC 1 cut(s) 1142
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 181, 545, 1315
EarI CTCTTC 3 cut(s) 181, 545, 1315
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 784, 919, 1818
Eco147I AGGCCT 1 cut(s) 908
Eco24I GRGCYC 3 cut(s) 237, 1217, 1872
Eco32I GATATC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 1027
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 413, 1296
Eco91I GGTNACC 1 cut(s) 914
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 3 cut(s) 350, 1027, 1971
EcoO65I GGTNACC 1 cut(s) 914
EcoRII CCWGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
EcoRV GATATC 1 cut(s) 131
EcoT14I CCWWGG 3 cut(s) 784, 919, 1818
EcoT38I GRGCYC 3 cut(s) 237, 1217, 1872
ErhI CCWWGG 3 cut(s) 784, 919, 1818
FaeI CATG 5 cut(s) 79, 112, 118, 626, 1822
FaqI GGGAC 1 cut(s) 1084
FatI CATG 5 cut(s) 75, 108, 114, 622, 1818
FbaI TGATCA 1 cut(s) 1962
Fnu4HI GCNGC 6 cut(s) 6, 534, 828, 1326, 1329, 1669
FokI GGATG 5 cut(s) 281, 875, 1112, 1540, 1844
FriOI GRGCYC 3 cut(s) 237, 1217, 1872
Fsp4HI GCNGC 6 cut(s) 6, 534, 828, 1326, 1329, 1669
FspBI CTAG 2 cut(s) 72, 318
GluI GCNGC 6 cut(s) 6, 534, 828, 1326, 1329, 1669
GsaI CCCAGC 2 cut(s) 1071, 1521
GsuI CTGGAG 2 cut(s) 83, 369
HaeIII GGCC 5 cut(s) 5, 352, 908, 975, 1973
HapII CCGG 2 cut(s) 63, 900
Hin1II CATG 5 cut(s) 79, 112, 118, 626, 1822
HpaII CCGG 2 cut(s) 63, 900
HphI GGTGA 3 cut(s) 889, 1252, 1274
Hpy188I TCNGA 7 cut(s) 393, 409, 718, 961, 1135, 1813, 1874
Hpy188III TCNNGA 9 cut(s) 34, 63, 92, 100, 623, 773, 807, 1030, 1779
HpyAV CCTTC 8 cut(s) 26, 187, 295, 668, 841, 1018, 1560, 1978
HpyCH4III ACNGT 6 cut(s) 677, 1255, 1457, 1792, 1885, 1939
HpyCH4IV ACGT 3 cut(s) 476, 1400, 1743
HpyCH4V TGCA 9 cut(s) 251, 526, 601, 740, 839, 955, 1157, 1668, 1689
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 727, 836, 1322, 1952
HpyF3I CTNAG 4 cut(s) 240, 354, 582, 1031
HpySE526I ACGT 3 cut(s) 476, 1400, 1743
Hsp92II CATG 5 cut(s) 79, 112, 118, 626, 1822
Kpn2I TCCGGA 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 1962
Kzo9I GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 4 cut(s) 208, 421, 1875, 1923
Lsp1109I GCAGC 5 cut(s) 520, 839, 1315, 1337, 1680
LweI GCATC 9 cut(s) 438, 588, 755, 770, 826, 952, 1090, 1822, 1935
MaeI CTAG 2 cut(s) 72, 318
MaeII ACGT 3 cut(s) 476, 1400, 1743
MaeIII GTNAC 4 cut(s) 914, 1280, 1850, 1997
MalI GATC 8 cut(s) 96, 457, 701, 951, 1293, 1783, 1815, 1964
MbiI CCGCTC 1 cut(s) 1697
MboI GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
MboII GAAGA 7 cut(s) 198, 562, 1179, 1249, 1253, 1302, 1887
MfeI CAATTG 1 cut(s) 1863
MflI RGATCY 3 cut(s) 455, 949, 1781
MhlI GDGCHC 3 cut(s) 237, 1217, 1872
MluCI AATT 9 cut(s) 11, 207, 273, 979, 1489, 1522, 1769, 1863, 1891
MlyI GAGTC 4 cut(s) 92, 788, 1288, 1692
MmeI TCCRAC 2 cut(s) 1549, 1809
MroI TCCGGA 1 cut(s) 62
MseI TTAA 8 cut(s) 371, 539, 870, 978, 1397, 1485, 1979, 1991
MslI CAYNNNNRTG 2 cut(s) 113, 1287
MspA1I CMGCKG 2 cut(s) 1067, 1328
MspI CCGG 2 cut(s) 63, 900
MspR9I CCNGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
MunI CAATTG 1 cut(s) 1863
MvaI CCWGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
MwoI GCNNNNNNNGC 5 cut(s) 578, 727, 836, 1322, 1952
NcoI CCATGG 1 cut(s) 1818
NdeII GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 5 cut(s) 79, 112, 118, 626, 1822
NlaIV GGNNCC 4 cut(s) 446, 1015, 1783, 1871
NmuCI GTSAC 2 cut(s) 1280, 1997
NspV TTCGAA 1 cut(s) 845
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 908
PciSI GCTCTTC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 3 cut(s) 305, 695, 1397
PfeI GAWTC 6 cut(s) 344, 358, 626, 755, 1121, 1775
PflMI CCANNNNNTGG 1 cut(s) 806
PfoI TCCNGGA 2 cut(s) 181, 1784
PkrI GCNGC 6 cut(s) 7, 535, 829, 1327, 1330, 1670
PleI GAGTC 4 cut(s) 91, 788, 1287, 1691
PpsI GAGTC 4 cut(s) 91, 788, 1287, 1691
Ppu21I YACGTR 1 cut(s) 477
PpuMI RGGWCCY 1 cut(s) 1027
PsiI TTATAA 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 1027
Psp6I CCWGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
PspEI GGTNACC 1 cut(s) 914
PspFI CCCAGC 2 cut(s) 1067, 1517
PspGI CCWGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
PspN4I GGNNCC 4 cut(s) 446, 1015, 1783, 1871
PspPI GGNCC 3 cut(s) 350, 1027, 1971
PspPPI RGGWCCY 1 cut(s) 1027
PstI CTGCAG 3 cut(s) 253, 528, 957
PstNI CAGNNNCTG 2 cut(s) 92, 353
PsuI RGATCY 3 cut(s) 455, 949, 1781
PvuII CAGCTG 2 cut(s) 1067, 1328
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 2 cut(s) 113, 1287
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 8 cut(s) 371, 539, 870, 978, 1397, 1485, 1979, 1991
SatI GCNGC 6 cut(s) 6, 534, 828, 1326, 1329, 1669
Sau3AI GATC 8 cut(s) 94, 455, 699, 949, 1291, 1781, 1813, 1962
Sau96I GGNCC 3 cut(s) 350, 1027, 1971
SbfI CCTGCAGG 1 cut(s) 528
SchI GAGTC 4 cut(s) 92, 788, 1288, 1692
ScrFI CCNGG 6 cut(s) 183, 910, 1173, 1464, 1610, 1786
SdaI CCTGCAGG 1 cut(s) 528
SduI GDGCHC 3 cut(s) 237, 1217, 1872
SfaNI GCATC 9 cut(s) 438, 588, 755, 770, 826, 952, 1090, 1822, 1935
SfcI CTRYAG 3 cut(s) 249, 524, 953
SfuI TTCGAA 1 cut(s) 845
SinI GGWCC 1 cut(s) 1027
SmiMI CAYNNNNRTG 2 cut(s) 113, 1287
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse8387I CCTGCAGG 1 cut(s) 528
Sse9I AATT 9 cut(s) 11, 207, 273, 979, 1489, 1522, 1769, 1863, 1891
SseBI AGGCCT 1 cut(s) 908
SsiI CCGC 2 cut(s) 6, 1697
SspI AATATT 3 cut(s) 706, 856, 1909
SspMI CTAG 2 cut(s) 72, 318
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 908
StyD4I CCNGG 6 cut(s) 181, 908, 1171, 1462, 1608, 1784
StyI CCWWGG 3 cut(s) 784, 919, 1818
TaaI ACNGT 6 cut(s) 677, 1255, 1457, 1792, 1885, 1939
TaiI ACGT 3 cut(s) 479, 1403, 1746
TaqI TCGA 5 cut(s) 142, 299, 845, 1005, 1902
TasI AATT 9 cut(s) 11, 207, 273, 979, 1489, 1522, 1769, 1863, 1891
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 6 cut(s) 344, 358, 626, 755, 1121, 1775
Tru1I TTAA 8 cut(s) 371, 539, 870, 978, 1397, 1485, 1979, 1991
Tru9I TTAA 8 cut(s) 371, 539, 870, 978, 1397, 1485, 1979, 1991
TscAI CASTG 4 cut(s) 270, 682, 1372, 1428
TseFI GTSAC 2 cut(s) 1280, 1997
TseI GCWGC 5 cut(s) 533, 827, 1325, 1328, 1668
Tsp45I GTSAC 2 cut(s) 1280, 1997
TspDTI ATGAA 8 cut(s) 38, 285, 623, 699, 807, 1049, 1542, 1560
TspRI CASTG 4 cut(s) 270, 682, 1372, 1428
Van91I CCANNNNNTGG 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 1027
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 3 cut(s) 11, 1769, 1891
XcmI CCANNNNNNNNNTGG 2 cut(s) 1825, 1939
XspI CTAG 2 cut(s) 72, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.