Prupe.3G165300_v2.0.a1

Nicastrin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0012086
Physical Location & Seq
Reverse (-)
18421461 .. 18432305
10845 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G165300.2

Sequence Viewer

Length: 2013 bp
ATGGCCATGAAATTCCTTCAACTGCTTTTCTTTTTCAGTTTTCAGCTTCGTCTCTCATTCTCTGGACAGACAATCATGATGGAGTCAGTTCCTGATCTTCAGAGTTCAATGTACATGACACTTGATGGATATCCTTGTGTACGGCTACTCAATCTTTCTGGTGTGATTGGTTGTTCAAATCCTGGAAGAGTGAAGGTTGTCGCTCCGATTATAAGATTAAACAATGCTACTGAGCTGTCTCAGTTATCTGCAGTTTTGCTGTCAGTGGATGAGATTCAAAGTTTTCTTACCAGAATATTGAATGATTCGACTTTTGCTAAAAATGTTGCTGGTATCTTGGTTGAATCAAGCCCTGAGTTCCAAAATAAGTTAAAAGGATTCTCTCCAGATCAAAAGTTCCCACAATCTGAATTTGCTCCTTATCAAAGCATCAACTACGAATGGAACCCAATTGGCTCTGGTATAATGTGGAACCCATATAACTTTCCTGTATTCTTACTCTCTCAGAGTAGCACATTGACCCTGCAGGAGGCTGCCATAAAGAATGAGAAGAACATAAAATCTTATACTGCTGACGTGGCTGAGTTTGATCTGGTGATGCAGACAATGAAAGCTGGGACTCATGATTCAGAATCTTGTCTAAAGGAAAATACTTGCCTTCCCTTAGGTGGATACAGTGTTTGGTCATCGCTTCCCCCAATCAATTTTTCATCGTCAGAGCAGTCTAAGCCCATTATACTCGTGGTGGCATCAATGGATTCAGCTTCATTTTTCCGTGACAAAGGCCTTGGCGCTGACTCCCCTATTTCTGGGCTGATTTCATTGCTGGCAGCAGTTGATGCACTTTCTCATGTGGATGGTCTGGATGACTTTAATAAACAGCTTGTTTTCATTGTTTTCACTGGAGAGGCCTGGGGCTACCTTGGTAGCAGGAGGTTTTTGCTTGAACTTGATCTGCAGTCTGATGCTGTTAGTGGCCTTAATTACTCATTGATTGAGAAGGTTGTGGAAATTGGATCTGTTGGAAAGGGCCTCAACCAAGGAGTTAAGAACTTTTTTGTTCATACAACTGGGGTTTCTTCTGCCACAAATGAGACATTGGATGCTTTAAAGCGTGCCCAAGATTCTGTCAAGTCTGAAAGCTTTACAATCTCATCTGCAAATGCTTCAAATCCTGGGATACCTCCATCCTCATTGATGACTTTTCTGAGAAAGAACTCTCTAACTTCTGGGGTTGTTTTAGAAGATTTTGATACTGTCTTCACCAACAAATTCTACAACAGTCACCTTGATGATATATCCAATGTAAACTCTTCAGCAATTGTGGCAGCAGCTTCCCTTGTAGCACGCACCCTTTACATTCTTGCAAGTGACAACAAAAATTTAAGCAGTTCAGCTATAACATCTATCAACGTGAATGTTTCACTAGTTGAAGAACTAATGGGTTGCCTATTGGACTGTGAGCCAGGTCTTTCTTGTGAGCTGGTGAAAAGCTATATATCACCGGCCAATACCTGCCCGAGCCATTATGTTGGTGTCATCCTTGGTGAACCTTCCTCCCCACCTTATCTTGGATATGTTGATGACATTTCAAGGTTCGTTTGGAATTTCCTGGCCGAAAAAACTTCCATTCCAAGGAAAAATGGGAGTTCTGTTTGTTCTCAAGACTGCAGTAATGAGGGTGAAGTATGCATTAGAGCAGAAACTGAGGGAAAGGGAGTCTGCGTTGTCTCCACAACCAGATATGTTCCAGCATACTCAACACGGTTGAAATATGAATCTGAATCTGGAACTTGGAATGTGTTGCCTCCAAATAACTCAGACCCCATGGGGCTGGTGGACGCTGTTTGGACAGAAAGCAACTGGGATACGATTGGACTCCGGGTCTACACCGTCCAAAATGCTTCTTTCGATCGTCTTGTCTTACTTGGGAGCATTGCCATTACAGTTTTGTCTTGCTTTGCAATAGTGATTACAAAGGCCTTCGTGACAAAGGCTGTAAAACGTGATTGA

Protein Analysis

671

Amino Acids

72.91

Weight (kDa)

4.93

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52640 AT3G52640 AT3G52640
fragaria_vesca FvH4_1g30060
malus_domestica MD17G1267200.v1.1
prunus_persica Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis pycom17g26630
rosa_chinensis RchiOBHm_Chr3g0492061
rosa_laevigata RLG00000022797
rosa_multiflora Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii Rroxscaffold_6G00392600
rosa_rugosa Rorug03G0251500
rosa_samantha Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana Rw3G026630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 1523
AccI GTMKAC 1 cut(s) 1887
AclWI GGATC 1 cut(s) 1024
AcoI YGGCCR 3 cut(s) 3, 1506, 1614
AcsI RAATTY 5 cut(s) 11, 410, 1271, 1381, 1606
AcuI CTGAAG 2 cut(s) 83, 1299
AfaI GTAC 2 cut(s) 113, 141
AfiI CCNNNNNNNGG 5 cut(s) 529, 668, 809, 1571, 1635
AhdI GACNNNNNGTC 1 cut(s) 1883
AhlI ACTAGT 1 cut(s) 1426
AjiI CACGTC 1 cut(s) 577
AjnI CCWGG 5 cut(s) 181, 911, 1174, 1465, 1611
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
Alw26I GTCTC 4 cut(s) 56, 243, 1088, 1735
AlwI GGATC 1 cut(s) 1024
AlwNI CAGNNNCTG 2 cut(s) 92, 1706
Ama87I CYCGRG 1 cut(s) 1519
AoxI GGCC 8 cut(s) 3, 784, 909, 976, 1030, 1506, 1614, 1980
ApeKI GCWGC 4 cut(s) 533, 830, 1328, 1331
ApoI RAATTY 5 cut(s) 11, 410, 1271, 1381, 1606
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 1 cut(s) 1030
AsuC2I CCSGG 1 cut(s) 1883
AsuHPI GGTGA 7 cut(s) 607, 1255, 1277, 1494, 1498, 1559, 1694
AvaI CYCGRG 1 cut(s) 1519
AxyI CCTNAGG 1 cut(s) 664
BaeGI GKGCMC 1 cut(s) 1120
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 1252
BbvI GCAGC 4 cut(s) 520, 842, 1340, 1343
BccI CCATC 4 cut(s) 73, 119, 851, 1195
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 5 cut(s) 183, 913, 1176, 1467, 1613
BciVI GTATCC 3 cut(s) 665, 1173, 1861
BcnI CCSGG 1 cut(s) 1883
BcoDI GTCTC 4 cut(s) 56, 243, 1088, 1735
BcuI ACTAGT 1 cut(s) 1426
BfaI CTAG 1 cut(s) 1427
BfmI CTRYAG 4 cut(s) 249, 524, 956, 1669
BfoI RGCGCY 1 cut(s) 795
BfuAI ACCTGC 1 cut(s) 1523
BfuI GTATCC 3 cut(s) 665, 1173, 1861
BisI GCNGC 4 cut(s) 534, 831, 1329, 1332
BlsI GCNGC 4 cut(s) 535, 832, 1330, 1333
Bme1390I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1883
BmeRI GACNNNNNGTC 1 cut(s) 1883
BmeT110I CYCGRG 1 cut(s) 1519
BmgBI CACGTC 1 cut(s) 577
BmgT120I GGNCC 1 cut(s) 1030
BmiI GGNNCC 2 cut(s) 446, 473
BmrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1883
BmrI ACTGGG 2 cut(s) 1080, 1873
BmsI GCATC 6 cut(s) 438, 588, 758, 829, 955, 1093
BmuI ACTGGG 2 cut(s) 1080, 1873
BpiI GAAGAC 1 cut(s) 1252
BpmI CTGGAG 2 cut(s) 369, 924
BpuEI CTTGAG 1 cut(s) 1647
BpuMI CCSGG 1 cut(s) 1883
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 8 cut(s) 787, 912, 922, 1039, 1175, 1543, 1634, 1827
BsaXI ACNNNNNCTCC 2 cut(s) 1710, 1740
Bsc4I CCNNNNNNNGG 5 cut(s) 529, 668, 809, 1571, 1635
Bse118I RCCGGY 1 cut(s) 1504
Bse1I ACTGG 3 cut(s) 907, 1075, 1868
Bse21I CCTNAGG 1 cut(s) 664
Bse3DI GCAATG 2 cut(s) 821, 1935
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 5 cut(s) 183, 913, 1176, 1467, 1613
BseDI CCNNGG 8 cut(s) 787, 912, 922, 1039, 1175, 1543, 1634, 1827
BseGI GGATG 6 cut(s) 274, 862, 871, 1108, 1187, 1539
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 5 cut(s) 529, 668, 809, 1571, 1635
BseMI GCAATG 2 cut(s) 821, 1935
BseMII CTCAG 8 cut(s) 222, 254, 345, 518, 573, 1199, 1698, 1833
BseNI ACTGG 3 cut(s) 907, 1075, 1868
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 4 cut(s) 520, 842, 1340, 1343
BseYI CCCAGC 1 cut(s) 614
Bsh1285I CGRYCG 1 cut(s) 1915
BshFI GGCC 8 cut(s) 5, 786, 911, 978, 1032, 1508, 1616, 1982
BsiEI CGRYCG 1 cut(s) 1915
BsiHKCI CYCGRG 1 cut(s) 1519
BsiSI CCGG 2 cut(s) 1505, 1882
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 5 cut(s) 529, 668, 809, 1571, 1635
BsmAI GTCTC 4 cut(s) 56, 243, 1088, 1735
BsmBI CGTCTC 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 631
BsnI GGCC 8 cut(s) 5, 786, 911, 978, 1032, 1508, 1616, 1982
BsoBI CYCGRG 1 cut(s) 1519
Bsp1286I GDGCHC 1 cut(s) 1120
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
Bsp19I CCATGG 1 cut(s) 1827
BspANI GGCC 8 cut(s) 5, 786, 911, 978, 1032, 1508, 1616, 1982
BspCNI CTCAG 8 cut(s) 223, 253, 346, 517, 574, 1200, 1699, 1832
BspHI TCATGA 2 cut(s) 75, 622
BspLI GGNNCC 2 cut(s) 446, 473
BspMAI CTGCAG 4 cut(s) 253, 528, 960, 1673
BspMI ACCTGC 1 cut(s) 1523
BspPI GGATC 1 cut(s) 1024
BsrDI GCAATG 2 cut(s) 821, 1935
BsrFI RCCGGY 1 cut(s) 1504
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 907, 1075, 1868
BssAI RCCGGY 1 cut(s) 1504
BssECI CCNNGG 8 cut(s) 787, 912, 922, 1039, 1175, 1543, 1634, 1827
BssMI GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
BssSI CACGAG 1 cut(s) 740
BssT1I CCWWGG 6 cut(s) 787, 922, 1039, 1543, 1634, 1827
Bst2BI CACGAG 1 cut(s) 740
Bst2UI CCWGG 5 cut(s) 183, 913, 1176, 1467, 1613
Bst4CI ACNGT 7 cut(s) 677, 1258, 1283, 1460, 1767, 1894, 1948
Bst6I CTCTTC 2 cut(s) 181, 1318
BstAPI GCANNNNNTGC 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 828, 1116, 1348
BstDSI CCRYGG 1 cut(s) 1827
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 6 cut(s) 274, 862, 871, 1108, 1187, 1539
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 6 cut(s) 97, 391, 592, 955, 1019, 1915
BstMAI GTCTC 4 cut(s) 56, 243, 1088, 1735
BstMBI GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
BstMCI CGRYCG 1 cut(s) 1915
BstMWI GCNNNNNNNGC 4 cut(s) 578, 727, 839, 1325
BstNI CCWGG 5 cut(s) 183, 913, 1176, 1467, 1613
BstSCI CCNGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1881
BstSFI CTRYAG 4 cut(s) 249, 524, 956, 1669
BstSLI GKGCMC 1 cut(s) 1120
BstV1I GCAGC 4 cut(s) 520, 842, 1340, 1343
BstV2I GAAGAC 1 cut(s) 1252
BstX2I RGATCY 1 cut(s) 1016
BstXI CCANNNNNNTGG 2 cut(s) 1532, 1834
BstYI RGATCY 1 cut(s) 1016
Bsu36I CCTNAGG 1 cut(s) 664
BsuI GTATCC 3 cut(s) 665, 1173, 1861
BsuRI GGCC 8 cut(s) 5, 786, 911, 978, 1032, 1508, 1616, 1982
BtgI CCRYGG 1 cut(s) 1827
BtgZI GCGATG 1 cut(s) 672
BtrI CACGTC 1 cut(s) 577
BtsCI GGATG 6 cut(s) 274, 862, 871, 1108, 1187, 1539
BtsIMutI CAGTG 3 cut(s) 270, 682, 900
BveI ACCTGC 1 cut(s) 1523
Cac8I GCNNGC 3 cut(s) 828, 1116, 1348
CaiI CAGNNNCTG 2 cut(s) 92, 1706
CciI TCATGA 2 cut(s) 75, 622
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 1504
Cfr13I GGNCC 1 cut(s) 1030
CseI GACGC 1 cut(s) 1850
Csp6I GTAC 2 cut(s) 112, 140
CviAII CATG 6 cut(s) 7, 76, 115, 623, 851, 1828
CviQI GTAC 2 cut(s) 112, 140
DpnI GATC 6 cut(s) 96, 390, 591, 954, 1018, 1914
DpnII GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
DraI TTTAAA 1 cut(s) 1110
DriI GACNNNNNGTC 1 cut(s) 1883
EaeI YGGCCR 3 cut(s) 3, 1506, 1614
Eam1104I CTCTTC 2 cut(s) 181, 1318
Eam1105I GACNNNNNGTC 1 cut(s) 1883
EarI CTCTTC 2 cut(s) 181, 1318
Eco130I CCWWGG 6 cut(s) 787, 922, 1039, 1543, 1634, 1827
Eco147I AGGCCT 3 cut(s) 786, 911, 1982
Eco32I GATATC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 83, 1299
Eco81I CCTNAGG 1 cut(s) 664
Eco88I CYCGRG 1 cut(s) 1519
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 1 cut(s) 1030
EcoRII CCWGG 5 cut(s) 181, 911, 1174, 1465, 1611
EcoRV GATATC 1 cut(s) 131
EcoT14I CCWWGG 6 cut(s) 787, 922, 1039, 1543, 1634, 1827
EcoT22I ATGCAT 1 cut(s) 1694
ErhI CCWWGG 6 cut(s) 787, 922, 1039, 1543, 1634, 1827
Esp3I CGTCTC 1 cut(s) 56
FaeI CATG 6 cut(s) 10, 79, 118, 626, 854, 1831
FaqI GGGAC 1 cut(s) 631
FatI CATG 6 cut(s) 6, 75, 114, 622, 850, 1827
FblI GTMKAC 1 cut(s) 1887
Fnu4HI GCNGC 4 cut(s) 534, 831, 1329, 1332
FokI GGATG 6 cut(s) 281, 869, 878, 1115, 1174, 1526
Fsp4HI GCNGC 4 cut(s) 534, 831, 1329, 1332
FspBI CTAG 1 cut(s) 1427
GlaI GCGC 1 cut(s) 793
GluI GCNGC 4 cut(s) 534, 831, 1329, 1332
GsaI CCCAGC 1 cut(s) 618
GsuI CTGGAG 2 cut(s) 369, 924
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 8 cut(s) 5, 786, 911, 978, 1032, 1508, 1616, 1982
HapII CCGG 2 cut(s) 1505, 1882
HgaI GACGC 1 cut(s) 1850
HhaI GCGC 1 cut(s) 794
Hin1II CATG 6 cut(s) 10, 79, 118, 626, 854, 1831
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HindIII AAGCTT 1 cut(s) 1141
HpaII CCGG 2 cut(s) 1505, 1882
HphI GGTGA 7 cut(s) 607, 1255, 1277, 1494, 1498, 1559, 1694
Hpy166II GTNNAC 5 cut(s) 140, 1309, 1550, 1840, 1888
Hpy188III TCNNGA 9 cut(s) 63, 76, 92, 386, 623, 863, 1664, 1788, 1987
Hpy8I GTNNAC 5 cut(s) 140, 1309, 1550, 1840, 1888
HpyAV CCTTC 6 cut(s) 26, 187, 668, 994, 1563, 1993
HpyCH4III ACNGT 7 cut(s) 677, 1258, 1283, 1460, 1767, 1894, 1948
HpyCH4IV ACGT 3 cut(s) 576, 1413, 2005
HpyF10VI GCNNNNNNNGC 4 cut(s) 578, 727, 839, 1325
HpySE526I ACGT 3 cut(s) 576, 1413, 2005
Hsp92II CATG 6 cut(s) 10, 79, 118, 626, 854, 1831
HspAI GCGC 1 cut(s) 792
Kzo9I GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
LmnI GCTCC 3 cut(s) 208, 421, 1932
Lsp1109I GCAGC 4 cut(s) 520, 842, 1340, 1343
LweI GCATC 6 cut(s) 438, 588, 758, 829, 955, 1093
MaeI CTAG 1 cut(s) 1427
MaeII ACGT 3 cut(s) 576, 1413, 2005
MaeIII GTNAC 4 cut(s) 776, 1283, 1370, 1987
MalI GATC 6 cut(s) 96, 390, 591, 954, 1018, 1914
MboI GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
MboII GAAGA 8 cut(s) 89, 198, 562, 1071, 1252, 1256, 1305, 1445
MfeI CAATTG 2 cut(s) 450, 1320
MflI RGATCY 1 cut(s) 1016
MhlI GDGCHC 1 cut(s) 1120
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 92, 613, 791, 1728, 1872
MmeI TCCRAC 1 cut(s) 1003
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1694
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 218, 371, 873, 981, 1047, 1109, 1385
MslI CAYNNNNRTG 2 cut(s) 855, 1290
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 1505, 1882
MspR9I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1883
MunI CAATTG 2 cut(s) 450, 1320
MvaI CCWGG 5 cut(s) 183, 913, 1176, 1467, 1613
MwoI GCNNNNNNNGC 4 cut(s) 578, 727, 839, 1325
NciI CCSGG 1 cut(s) 1883
NcoI CCATGG 1 cut(s) 1827
NdeII GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
NlaIII CATG 6 cut(s) 10, 79, 118, 626, 854, 1831
NlaIV GGNNCC 2 cut(s) 446, 473
NmuCI GTSAC 4 cut(s) 776, 1283, 1370, 1987
NsiI ATGCAT 1 cut(s) 1694
PagI TCATGA 2 cut(s) 75, 622
PceI AGGCCT 3 cut(s) 786, 911, 1982
PfoI TCCNGGA 1 cut(s) 181
PkrI GCNGC 4 cut(s) 535, 832, 1330, 1333
Ple19I CGATCG 1 cut(s) 1915
PleI GAGTC 5 cut(s) 91, 613, 791, 1727, 1872
PpsI GAGTC 5 cut(s) 91, 613, 791, 1727, 1872
PsiI TTATAA 1 cut(s) 212
Psp6I CCWGG 5 cut(s) 181, 911, 1174, 1465, 1611
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 5 cut(s) 181, 911, 1174, 1465, 1611
PspN4I GGNNCC 2 cut(s) 446, 473
PspPI GGNCC 1 cut(s) 1030
PstI CTGCAG 4 cut(s) 253, 528, 960, 1673
PstNI CAGNNNCTG 2 cut(s) 92, 1706
PsuI RGATCY 1 cut(s) 1016
PvuI CGATCG 1 cut(s) 1915
RsaI GTAC 2 cut(s) 113, 141
RsaNI GTAC 2 cut(s) 112, 140
RseI CAYNNNNRTG 2 cut(s) 855, 1290
SaqAI TTAA 7 cut(s) 218, 371, 873, 981, 1047, 1109, 1385
SatI GCNGC 4 cut(s) 534, 831, 1329, 1332
Sau3AI GATC 6 cut(s) 94, 388, 589, 952, 1016, 1912
Sau96I GGNCC 1 cut(s) 1030
SbfI CCTGCAGG 1 cut(s) 528
SchI GAGTC 5 cut(s) 92, 613, 791, 1728, 1872
ScrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1613, 1883
SdaI CCTGCAGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 1120
SfaNI GCATC 6 cut(s) 438, 588, 758, 829, 955, 1093
SfcI CTRYAG 4 cut(s) 249, 524, 956, 1669
SmiMI CAYNNNNRTG 2 cut(s) 855, 1290
SmlI CTYRAG 1 cut(s) 1662
SmoI CTYRAG 1 cut(s) 1662
SpeI ACTAGT 1 cut(s) 1426
Sse8387I CCTGCAGG 1 cut(s) 528
SseBI AGGCCT 3 cut(s) 786, 911, 1982
SspI AATATT 1 cut(s) 297
SspMI CTAG 1 cut(s) 1427
StuI AGGCCT 3 cut(s) 786, 911, 1982
StyD4I CCNGG 6 cut(s) 181, 911, 1174, 1465, 1611, 1881
StyI CCWWGG 6 cut(s) 787, 922, 1039, 1543, 1634, 1827
TaaI ACNGT 7 cut(s) 677, 1258, 1283, 1460, 1767, 1894, 1948
TaiI ACGT 3 cut(s) 579, 1416, 2008
TaqI TCGA 2 cut(s) 308, 1911
TatI WGTACW 1 cut(s) 111
Tru1I TTAA 7 cut(s) 218, 371, 873, 981, 1047, 1109, 1385
Tru9I TTAA 7 cut(s) 218, 371, 873, 981, 1047, 1109, 1385
TscAI CASTG 3 cut(s) 270, 682, 907
TseFI GTSAC 4 cut(s) 776, 1283, 1370, 1987
TseI GCWGC 4 cut(s) 533, 830, 1328, 1331
Tsp45I GTSAC 4 cut(s) 776, 1283, 1370, 1987
TspDTI ATGAA 8 cut(s) 23, 623, 699, 756, 810, 880, 1052, 1791
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 3 cut(s) 270, 682, 907
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 5 cut(s) 11, 410, 1271, 1381, 1606
XcmI CCANNNNNNNNNTGG 2 cut(s) 739, 1834
XmiI GTMKAC 1 cut(s) 1887
XspI CTAG 1 cut(s) 1427
Zsp2I ATGCAT 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.