RLG00000022797

Nicastrin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012086
Physical Location & Seq
Forward (+)
12331180 .. 12347694
16515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022797

Sequence Viewer

Length: 1998 bp
ATGGCCACCAAATTCCTTCAACTGCTTTTGCTCTTGAGTTTTCAGCTTCATCTATCTTTCTCCGGACAGACTAGCATGATGGAGTCAGTTCCTGATCTCCAGAGTTCCATGTACAAGACGATTGATGGATATCCTTGTGTTCGACTACTCAATCTTTCTGGAGAGATTGGTTGTTCAAATCCTGGAAGAGAGAAGGTTGTTGCTCCAATTATAAGACTAAATAATGCTAATGAGCTCACTCAGTTATCTGCAGTTCTGCTTTCAGTGGATGAAATTGAAAGTTTTTTTGCCAGAATATTGAATGATACGACCTTTGCTAGCAATGTAGCTGGTGTCTTGGTAGAATCAGGGCCTGAGATTCAAAATAAGTTAAAAGGGTTCTCTCCAGCTCTAAAGTTCCCACAATCAGGTTTTGCTCCTTACAGAAGCATCAACTACGAATGGAACCCGATGGGATCTGGTATAATGTGGAACATGTATAACTTTCCTGTATTCTTACTCTCCCAGAGTAGCACATTGACCCTGCAGGAGGCTGCCTTAAACAATGAGAAGAGCAAGAAATCTTATACTGCTGATGTGGCTGAGTTTGATTTGGTGATGCAGACAATGAAAGCTGGCACTCATGATTCAAAATCTTGTTTAGAGGAGAATACTTGCCTTCCTTTAGGTGGATACAGTGTTTGGTCATCGCTTCCTCCGATCAATATTTCATCATCAGAGCAGTCCAAGCCCATTATACTTGCAGTAGCATCAATGGATTCAGCATCATTTTTTCGTGATAAAAACCTTGGTGCTGACTCCCCAATATCTGGACTGATTTCATTGCTGGCAGCTGTTGATGCACTTTCGAAGGGGAATATTGTGGATGACTTCAATAAACAGCTTGTATTTATTGTTTTCACCGGAGAGGCCTGGGGTTACCTTGGTAGCAGGAGATTTTTGCTGGAACTGGATCTGCAGTCTGATGCTGTTAGTGGCCTTAATTACTCATTGATTGAGAGGGTTGTCGAAATAGGTTCCATTGGGAAGGCCCTGAACCAAGAAGCTAAGAACTTTTTCGTTCATACAGCTGGGACTTCCTCTGCCACAAATGAGACATTGGATGCTTTGAGGATTGCCCAAGATTCAGTCAAGTCTGACCACATTGTCGTCTTGTCTGCAAATGCTTCAAATCCTGGGATACCTCCATCTTCCTTGATGGCTTTTATGGGAAAGGACTCTCTAACCTCTGGGGTTGTATTGGAAGACTTTGATACTGTCTTCACCAACAAGTTCTACCAGAGTCACCTTGATGATCTATCAAATATAAACTCTTCAGCTATTGTGGCGGCTGCTTCCCTTGTAGCACGCACCCTTTACATTCTTGCCAGTGGCAATAAAAATACAAGCAGTTCAGCTTTAACGTCTATCAATGTGAATGTTTCACTGGTCGAAGAACTAATGGGTTGCTTATTGGACTGTGAGCCAGGTCTTTCTTGTGAGCTAGTAAAAAATTATATAACACCAGTGAATACCTGCCCCAGCAATTATGTTGGCGTTATCGTTGATGAACCTTCATCCATTGGATATGTTAGTGATATTTCAAGGTTTGTTTGGAACTTCCTGGCTGAAAAAACTTCTATTTCAAAGGAAAATGCTACCTCTGTTTGTTCTAAAGACTGCAGCAATGATGGTAGAGTCTGCATTAGAGCAGAGACAGATGGAAAGGGAGTTTGTGTTGTCTCCACAACCAGATACGTTCCAGCTTACTCAACAAGGTTGAAATTTGAATCTGGATCCTGGACTGTATTGCCTCCAAATAACTCTGATCCCATGGGGCTGTTGGATGCCGTTTGGACGGAAAGTAACTGGAATACAATTGGGCTCCGAGTCTATACTGTCCAAAATTCTTCTTTCGATAATATTGTTTTACTTGGAAGCATCGCCATTACAGTCTTGGCTTACCTCGCAATAGTGATTACAAGGGCCTTTTTAACAAAGGCTTTAAAGCGTGATTGA

Protein Analysis

666

Amino Acids

72.03

Weight (kDa)

4.92

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ncstrn_small PF18266 45 - 202 7.6e-43 Nicastrin small lobe
Peptidase_M28 PF04389 243 - 450 3.5e-06 Peptidase family M28
Nicastrin PF05450 244 - 453 1.2e-72 Nicastrin large lobe
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52640 AT3G52640 AT3G52640
fragaria_vesca FvH4_1g30060
malus_domestica MD17G1267200.v1.1
prunus_persica Prupe.3G165300_v2.0.a1 Prupe.3G165300_v2.0.a1
pyrus_communis pycom17g26630
rosa_chinensis RchiOBHm_Chr3g0492061
rosa_laevigata RLG00000022797
rosa_multiflora Rmu_sc0003846.1_g000020 Rmu_sc0042516.1_g000001
rosa_roxburghii Rroxscaffold_6G00392600
rosa_rugosa Rorug03G0251500
rosa_samantha Rh3AG301700 Rh3BG337500 Rh3CG335000 Rh3DG337100
rosa_wichuraiana Rw3G026630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 212
AasI GACNNNNNNGTC 1 cut(s) 1145
Acc36I ACCTGC 1 cut(s) 1523
AccB7I CCANNNNNTGG 1 cut(s) 809
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 1 cut(s) 1328
AclWI GGATC 5 cut(s) 463, 960, 1770, 1783, 1802
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 11, 1763, 1885
AcuI CTGAAG 1 cut(s) 1299
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 4 cut(s) 407, 529, 668, 809
AflIII ACRYGT 1 cut(s) 474
AjnI CCWGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 3 cut(s) 1088, 1688, 1726
AlwI GGATC 5 cut(s) 463, 960, 1770, 1783, 1802
AlwNI CAGNNNCTG 2 cut(s) 92, 353
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 6 cut(s) 3, 350, 909, 976, 1029, 1965
ApeKI GCWGC 4 cut(s) 533, 830, 1331, 1662
ApoI RAATTY 3 cut(s) 11, 1763, 1885
Asp700I GAANNNNTTC 1 cut(s) 1055
AspS9I GGNCC 3 cut(s) 350, 1030, 1965
AsuHPI GGTGA 4 cut(s) 607, 892, 1255, 1277
AsuII TTCGAA 1 cut(s) 848
AsuNHI GCTAGC 1 cut(s) 317
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1775
BanII GRGCYC 2 cut(s) 237, 1866
BbsI GAAGAC 2 cut(s) 1251, 1252
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 4 cut(s) 520, 842, 1318, 1674
BccI CCATC 7 cut(s) 73, 119, 445, 1192, 1195, 1664, 1694
BceAI ACGGC 1 cut(s) 1814
BciT130I CCWGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
BciVI GTATCC 2 cut(s) 665, 1173
BcoDI GTCTC 3 cut(s) 1088, 1688, 1726
BfaI CTAG 3 cut(s) 72, 318, 1484
BfmI CTRYAG 4 cut(s) 249, 524, 956, 1660
BfuAI ACCTGC 1 cut(s) 1523
BfuI GTATCC 2 cut(s) 665, 1173
BisI GCNGC 5 cut(s) 534, 831, 1329, 1332, 1663
BlsI GCNGC 5 cut(s) 535, 832, 1330, 1333, 1664
Bme1390I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
BmgT120I GGNCC 3 cut(s) 350, 1030, 1965
BmiI GGNNCC 4 cut(s) 446, 1018, 1777, 1865
BmrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
BmsI GCATC 9 cut(s) 438, 588, 758, 773, 829, 955, 1093, 1816, 1929
BmtI GCTAGC 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 1251, 1252
BpmI CTGGAG 3 cut(s) 83, 180, 369
Bpu14I TTCGAA 1 cut(s) 848
BpuEI CTTGAG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1812
BsaWI WCCGGW 2 cut(s) 62, 902
BsaXI ACNNNNNCTCC 2 cut(s) 1701, 1731
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 529, 668, 809
Bse1I ACTGG 5 cut(s) 954, 1368, 1431, 1505, 1853
Bse3DI GCAATG 3 cut(s) 328, 821, 1672
Bse8I GATNNNNATC 1 cut(s) 129
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
BseDI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1812
BseGI GGATG 5 cut(s) 274, 871, 1108, 1556, 1831
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 4 cut(s) 407, 529, 668, 809
BseMI GCAATG 3 cut(s) 328, 821, 1672
BseMII CTCAG 3 cut(s) 254, 345, 573
BseNI ACTGG 5 cut(s) 954, 1368, 1431, 1505, 1853
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 4 cut(s) 520, 842, 1318, 1674
BseYI CCCAGC 2 cut(s) 1070, 1520
BshFI GGCC 6 cut(s) 5, 352, 911, 978, 1031, 1967
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 2 cut(s) 63, 903
BslFI GGGAC 1 cut(s) 1087
BslI CCNNNNNNNGG 4 cut(s) 407, 529, 668, 809
BsmAI GTCTC 3 cut(s) 1088, 1688, 1726
BsmFI GGGAC 1 cut(s) 1087
BsnI GGCC 6 cut(s) 5, 352, 911, 978, 1031, 1967
Bsp119I TTCGAA 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 237, 1866
Bsp13I TCCGGA 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
Bsp19I CCATGG 1 cut(s) 1812
BspACI CCGC 1 cut(s) 1328
BspANI GGCC 6 cut(s) 5, 352, 911, 978, 1031, 1967
BspCNI CTCAG 3 cut(s) 253, 346, 574
BspEI TCCGGA 1 cut(s) 62
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 4 cut(s) 446, 1018, 1777, 1865
BspMAI CTGCAG 4 cut(s) 253, 528, 960, 1664
BspMI ACCTGC 1 cut(s) 1523
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 5 cut(s) 463, 960, 1770, 1783, 1802
BspQI GCTCTTC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 848
BsrDI GCAATG 3 cut(s) 328, 821, 1672
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 5 cut(s) 954, 1368, 1431, 1505, 1853
BssECI CCNNGG 5 cut(s) 787, 912, 922, 1175, 1812
BssMI GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
BssT1I CCWWGG 3 cut(s) 787, 922, 1812
Bst2UI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
Bst4CI ACNGT 6 cut(s) 677, 1258, 1460, 1786, 1879, 1933
Bst6I CTCTTC 3 cut(s) 181, 545, 1318
BstAUI TGTACA 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 848
BstC8I GCNNGC 4 cut(s) 319, 616, 828, 1348
BstDEI CTNAG 4 cut(s) 240, 354, 582, 1047
BstDSI CCRYGG 1 cut(s) 1812
BstEII GGTNACC 1 cut(s) 917
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 5 cut(s) 274, 871, 1108, 1556, 1831
BstKTI GATC 7 cut(s) 97, 458, 702, 955, 1297, 1778, 1810
BstMAI GTCTC 3 cut(s) 1088, 1688, 1726
BstMBI GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
BstMWI GCNNNNNNNGC 5 cut(s) 578, 727, 839, 1325, 1946
BstNI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
BstNSI RCATGY 1 cut(s) 478
BstPI GGTNACC 1 cut(s) 917
BstSCI CCNGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
BstSFI CTRYAG 4 cut(s) 249, 524, 956, 1660
BstV1I GCAGC 4 cut(s) 520, 842, 1318, 1674
BstV2I GAAGAC 2 cut(s) 1251, 1252
BstX2I RGATCY 3 cut(s) 455, 952, 1775
BstYI RGATCY 3 cut(s) 455, 952, 1775
BsuI GTATCC 2 cut(s) 665, 1173
BsuRI GGCC 6 cut(s) 5, 352, 911, 978, 1031, 1967
BtgI CCRYGG 1 cut(s) 1812
BtgZI GCGATG 2 cut(s) 672, 1906
BtsCI GGATG 5 cut(s) 274, 871, 1108, 1556, 1831
BtsIMutI CAGTG 5 cut(s) 270, 682, 1375, 1424, 1512
BveI ACCTGC 1 cut(s) 1523
Cac8I GCNNGC 4 cut(s) 319, 616, 828, 1348
CaiI CAGNNNCTG 2 cut(s) 92, 353
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 3 cut(s) 350, 1030, 1965
Csp6I GTAC 1 cut(s) 112
CviAII CATG 5 cut(s) 76, 109, 475, 623, 1813
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 4 cut(s) 240, 354, 582, 1047
DpnI GATC 7 cut(s) 96, 457, 701, 954, 1296, 1777, 1809
DpnII GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
DraI TTTAAA 1 cut(s) 1986
DrdI GACNNNNNNGTC 1 cut(s) 1145
DseDI GACNNNNNNGTC 1 cut(s) 1145
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 181, 545, 1318
EarI CTCTTC 3 cut(s) 181, 545, 1318
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 787, 922, 1812
Eco147I AGGCCT 1 cut(s) 911
Eco24I GRGCYC 2 cut(s) 237, 1866
Eco32I GATATC 1 cut(s) 131
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 1299
Eco91I GGTNACC 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 3 cut(s) 350, 1030, 1965
EcoO65I GGTNACC 1 cut(s) 917
EcoRII CCWGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
EcoRV GATATC 1 cut(s) 131
EcoT14I CCWWGG 3 cut(s) 787, 922, 1812
EcoT38I GRGCYC 2 cut(s) 237, 1866
ErhI CCWWGG 3 cut(s) 787, 922, 1812
FaeI CATG 5 cut(s) 79, 112, 478, 626, 1816
FaqI GGGAC 1 cut(s) 1087
FatI CATG 5 cut(s) 75, 108, 474, 622, 1812
Fnu4HI GCNGC 5 cut(s) 534, 831, 1329, 1332, 1663
FokI GGATG 5 cut(s) 281, 878, 1115, 1543, 1838
FriOI GRGCYC 2 cut(s) 237, 1866
Fsp4HI GCNGC 5 cut(s) 534, 831, 1329, 1332, 1663
FspBI CTAG 3 cut(s) 72, 318, 1484
GluI GCNGC 5 cut(s) 534, 831, 1329, 1332, 1663
GsaI CCCAGC 2 cut(s) 1074, 1524
GsuI CTGGAG 3 cut(s) 83, 180, 369
HaeIII GGCC 6 cut(s) 5, 352, 911, 978, 1031, 1967
HapII CCGG 2 cut(s) 63, 903
Hin1II CATG 5 cut(s) 79, 112, 478, 626, 1816
HpaII CCGG 2 cut(s) 63, 903
HphI GGTGA 4 cut(s) 607, 892, 1255, 1277
Hpy188I TCNGA 6 cut(s) 699, 718, 964, 1138, 1807, 1868
Hpy188III TCNNGA 9 cut(s) 34, 63, 92, 100, 159, 623, 776, 810, 1773
HpyAV CCTTC 6 cut(s) 26, 187, 668, 844, 1021, 1563
HpyCH4III ACNGT 6 cut(s) 677, 1258, 1460, 1786, 1879, 1933
HpyCH4IV ACGT 2 cut(s) 1403, 1737
HpyCH4V TGCA 9 cut(s) 251, 526, 601, 743, 842, 958, 1160, 1662, 1683
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 727, 839, 1325, 1946
HpyF3I CTNAG 4 cut(s) 240, 354, 582, 1047
HpySE526I ACGT 2 cut(s) 1403, 1737
Hsp92II CATG 5 cut(s) 79, 112, 478, 626, 1816
Kpn2I TCCGGA 1 cut(s) 62
Kzo9I GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
LguI GCTCTTC 1 cut(s) 545
LmnI GCTCC 3 cut(s) 208, 421, 1869
Lsp1109I GCAGC 4 cut(s) 520, 842, 1318, 1674
LweI GCATC 9 cut(s) 438, 588, 758, 773, 829, 955, 1093, 1816, 1929
MaeI CTAG 3 cut(s) 72, 318, 1484
MaeII ACGT 2 cut(s) 1403, 1737
MaeIII GTNAC 3 cut(s) 917, 1283, 1844
MalI GATC 7 cut(s) 96, 457, 701, 954, 1296, 1777, 1809
MboI GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
MboII GAAGA 8 cut(s) 198, 562, 1182, 1252, 1256, 1305, 1445, 1881
MfeI CAATTG 1 cut(s) 1857
MflI RGATCY 3 cut(s) 455, 952, 1775
MhlI GDGCHC 2 cut(s) 237, 1866
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 11, 207, 273, 982, 1492, 1525, 1763, 1857, 1885
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 92, 791, 1211, 1291, 1686, 1878
MmeI TCCRAC 1 cut(s) 1803
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 371, 539, 981, 1400, 1973, 1985
MslI CAYNNNNRTG 1 cut(s) 1290
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 833, 1070
MspI CCGG 2 cut(s) 63, 903
MspR9I CCNGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
MunI CAATTG 1 cut(s) 1857
MvaI CCWGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
MwoI GCNNNNNNNGC 5 cut(s) 578, 727, 839, 1325, 1946
NcoI CCATGG 1 cut(s) 1812
NdeII GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 5 cut(s) 79, 112, 478, 626, 1816
NlaIV GGNNCC 4 cut(s) 446, 1018, 1777, 1865
NmuCI GTSAC 1 cut(s) 1283
NspI RCATGY 1 cut(s) 478
NspV TTCGAA 1 cut(s) 848
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 911
PciI ACATGT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 695
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 6 cut(s) 344, 358, 626, 758, 1124, 1769
PflMI CCANNNNNTGG 1 cut(s) 809
PfoI TCCNGGA 2 cut(s) 181, 1778
PkrI GCNGC 5 cut(s) 535, 832, 1330, 1333, 1664
PleI GAGTC 6 cut(s) 91, 791, 1211, 1290, 1685, 1877
PpsI GAGTC 6 cut(s) 91, 791, 1211, 1290, 1685, 1877
PscI ACATGT 1 cut(s) 474
PsiI TTATAA 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 2 cut(s) 1070, 1520
PspGI CCWGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
PspN4I GGNNCC 4 cut(s) 446, 1018, 1777, 1865
PspPI GGNCC 3 cut(s) 350, 1030, 1965
PstI CTGCAG 4 cut(s) 253, 528, 960, 1664
PstNI CAGNNNCTG 2 cut(s) 92, 353
PsuI RGATCY 3 cut(s) 455, 952, 1775
PvuII CAGCTG 2 cut(s) 833, 1070
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 1290
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 545
SaqAI TTAA 6 cut(s) 371, 539, 981, 1400, 1973, 1985
SatI GCNGC 5 cut(s) 534, 831, 1329, 1332, 1663
Sau3AI GATC 7 cut(s) 94, 455, 699, 952, 1294, 1775, 1807
Sau96I GGNCC 3 cut(s) 350, 1030, 1965
SbfI CCTGCAGG 1 cut(s) 528
SchI GAGTC 6 cut(s) 92, 791, 1211, 1291, 1686, 1878
ScrFI CCNGG 6 cut(s) 183, 913, 1176, 1467, 1604, 1780
SdaI CCTGCAGG 1 cut(s) 528
SduI GDGCHC 2 cut(s) 237, 1866
SfaNI GCATC 9 cut(s) 438, 588, 758, 773, 829, 955, 1093, 1816, 1929
SfcI CTRYAG 4 cut(s) 249, 524, 956, 1660
SfuI TTCGAA 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 1290
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse8387I CCTGCAGG 1 cut(s) 528
Sse9I AATT 9 cut(s) 11, 207, 273, 982, 1492, 1525, 1763, 1857, 1885
SseBI AGGCCT 1 cut(s) 911
SsiI CCGC 1 cut(s) 1328
SspI AATATT 4 cut(s) 297, 706, 859, 1903
SspMI CTAG 3 cut(s) 72, 318, 1484
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 911
StyD4I CCNGG 6 cut(s) 181, 911, 1174, 1465, 1602, 1778
StyI CCWWGG 3 cut(s) 787, 922, 1812
TaaI ACNGT 6 cut(s) 677, 1258, 1460, 1786, 1879, 1933
TaiI ACGT 2 cut(s) 1406, 1740
TaqI TCGA 5 cut(s) 142, 848, 1008, 1431, 1896
TasI AATT 9 cut(s) 11, 207, 273, 982, 1492, 1525, 1763, 1857, 1885
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 6 cut(s) 344, 358, 626, 758, 1124, 1769
Tru1I TTAA 6 cut(s) 371, 539, 981, 1400, 1973, 1985
Tru9I TTAA 6 cut(s) 371, 539, 981, 1400, 1973, 1985
TscAI CASTG 5 cut(s) 270, 682, 1375, 1431, 1512
TseFI GTSAC 1 cut(s) 1283
TseI GCWGC 4 cut(s) 533, 830, 1331, 1662
Tsp45I GTSAC 1 cut(s) 1283
TspDTI ATGAA 8 cut(s) 38, 285, 623, 699, 810, 1052, 1545, 1563
TspGWI ACGGA 1 cut(s) 1853
TspRI CASTG 5 cut(s) 270, 682, 1375, 1431, 1512
Van91I CCANNNNNTGG 1 cut(s) 809
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 3 cut(s) 11, 1763, 1885
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 2 cut(s) 1819, 1933
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 3 cut(s) 72, 318, 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.