FvH4_1g30240

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013600
Physical Location & Seq
Forward (+)
23960738 .. 23962337
1600 bp
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UTR
Exon/CDS
Intron
FvH4_1g30240.t1

Sequence Viewer

Length: 711 bp
ATGGTTTTGATTTCCAAGGAAGAAAAGAAAAAAAAAGGATTCTGTTCCTTCTGTTTTAATTGCTTATTATATAAGGAAGACCCCGGCCTGCTGGTTTCGTTTAGCTCCAAAGAATCTCTTGTATCCGTAACCATGGTAGCTGTGGTATCATCTCAAGAAAACATGGTTTCTCGCACAGGAAGGCACCTGCAGCGCTACAACAAAGGTCGTCGCCAAGTCGTAGGATGTATTCCATATAGATACAAAATGGCCAAGCAGAGTCTCTCAAAGGATGATGGTGCAAAAGAACTACAAGTTCTTGTGATTACTTCACAAAAGGGCAAAGGGATGCTCTTCCCTAAGGGAGGGTGGGAATTAGATGAATCCAAGGAAGGGGCAGCCTCTCGAGAGACGCTGGAGGAAGCCGGGGTGCGAGGAACCATTGAGTGTGAATTGGGTAAGTGGAGCTTCAAGAGCAAATCCCATGATGCTCACTATGAAGGTTACATGTTCCCATTACATGTTCAGGAGCAGTTAGATTTCTGGCCAGAGAAGAATCTGCGCCAAAGAATATGGATGAGTGCACAGGAAGCAAGAGAAGTTTGTCAACACTGGTGGATGAAAGAAGCCTTGGATAGATTAGTAAATCGGCTCACATCTCAACAACAACAACAGCAACATGAAGACCAAGAATTAGGCCCTGTTCTTAAATCAATGAGGGCCAGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

237

Amino Acids

27.21

Weight (kDa)

8.94

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 96 - 205 1.8e-08 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 183
AcoI YGGCCR 2 cut(s) 249, 524
AfeI AGCGCT 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 344, 372
AflIII ACRYGT 2 cut(s) 486, 499
AgsI TTSAA 1 cut(s) 451
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 3 cut(s) 105, 140, 447
AluI AGCT 3 cut(s) 105, 140, 447
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 2 cut(s) 266, 383
Alw44I GTGCAC 1 cut(s) 561
Ama87I CYCGRG 1 cut(s) 384
Aor51HI AGCGCT 1 cut(s) 194
AoxI GGCC 5 cut(s) 85, 249, 524, 676, 699
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 2 cut(s) 190, 377
AspLEI GCGC 2 cut(s) 195, 543
AspS9I GGNCC 2 cut(s) 677, 699
AsuC2I CCSGG 2 cut(s) 84, 406
AvaI CYCGRG 1 cut(s) 384
AxyI CCTNAGG 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 565
BalI TGGCCA 2 cut(s) 251, 526
BanI GGYRCC 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 84, 669
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 2 cut(s) 202, 389
BccI CCATC 1 cut(s) 269
BciVI GTATCC 1 cut(s) 133
BcnI CCSGG 2 cut(s) 84, 406
BcoDI GTCTC 2 cut(s) 266, 383
BfmI CTRYAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 195
BfuI GTATCC 1 cut(s) 133
BisI GCNGC 2 cut(s) 191, 378
BlsI GCNGC 2 cut(s) 192, 379
Bme1390I CCNGG 2 cut(s) 84, 406
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 2 cut(s) 677, 699
BmiI GGNNCC 2 cut(s) 185, 418
BmrFI CCNGG 2 cut(s) 84, 406
BmsI GCATC 2 cut(s) 318, 457
BpiI GAAGAC 2 cut(s) 84, 669
BpmI CTGGAG 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 138
BpuMI CCSGG 2 cut(s) 84, 406
BsaJI CCNNGG 6 cut(s) 15, 82, 132, 366, 405, 609
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 372
Bse1I ACTGG 2 cut(s) 596, 702
Bse21I CCTNAGG 1 cut(s) 339
BseDI CCNNGG 6 cut(s) 15, 82, 132, 366, 405, 609
BseGI GGATG 5 cut(s) 230, 277, 333, 561, 603
BseLI CCNNNNNNNGG 2 cut(s) 344, 372
BseNI ACTGG 2 cut(s) 596, 702
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 2 cut(s) 202, 389
BshFI GGCC 5 cut(s) 87, 251, 526, 678, 701
BshNI GGYRCC 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 1 cut(s) 384
BsiSI CCGG 2 cut(s) 84, 405
BslI CCNNNNNNNGG 2 cut(s) 344, 372
BsmAI GTCTC 2 cut(s) 266, 383
BsmBI CGTCTC 1 cut(s) 383
BsnI GGCC 5 cut(s) 87, 251, 526, 678, 701
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 565
Bsp19I CCATGG 1 cut(s) 132
BspANI GGCC 5 cut(s) 87, 251, 526, 678, 701
BspLI GGNNCC 2 cut(s) 185, 418
BspMAI CTGCAG 1 cut(s) 192
BspMI ACCTGC 1 cut(s) 195
BspQI GCTCTTC 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 183
BsrI ACTGG 2 cut(s) 596, 702
BssECI CCNNGG 6 cut(s) 15, 82, 132, 366, 405, 609
BssT1I CCWWGG 4 cut(s) 15, 132, 366, 609
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 5 cut(s) 230, 277, 333, 561, 603
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 543
BstMAI GTCTC 2 cut(s) 266, 383
BstMWI GCNNNNNNNGC 3 cut(s) 190, 453, 569
BstNSI RCATGY 2 cut(s) 490, 503
BstSCI CCNGG 2 cut(s) 82, 404
BstSFI CTRYAG 1 cut(s) 188
BstSLI GKGCMC 1 cut(s) 565
BstV1I GCAGC 2 cut(s) 202, 389
BstV2I GAAGAC 2 cut(s) 84, 669
Bsu36I CCTNAGG 1 cut(s) 339
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 5 cut(s) 87, 251, 526, 678, 701
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 5 cut(s) 230, 277, 333, 561, 603
BtsIMutI CAGTG 1 cut(s) 589
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 89
CfoI GCGC 2 cut(s) 195, 543
Cfr13I GGNCC 2 cut(s) 677, 699
CseI GACGC 1 cut(s) 400
CspCI CAANNNNNGTGG 2 cut(s) 575, 610
CviAII CATG 6 cut(s) 133, 163, 464, 487, 500, 659
DdeI CTNAG 1 cut(s) 339
EaeI YGGCCR 2 cut(s) 249, 524
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 4 cut(s) 15, 132, 366, 609
Eco47III AGCGCT 1 cut(s) 194
Eco81I CCTNAGG 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 677
EcoT14I CCWWGG 4 cut(s) 15, 132, 366, 609
ErhI CCWWGG 4 cut(s) 15, 132, 366, 609
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 6 cut(s) 136, 166, 467, 490, 503, 662
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 431, 463
FatI CATG 6 cut(s) 132, 162, 463, 486, 499, 658
Fnu4HI GCNGC 2 cut(s) 191, 378
FokI GGATG 5 cut(s) 237, 284, 340, 568, 610
Fsp4HI GCNGC 2 cut(s) 191, 378
GlaI GCGC 2 cut(s) 194, 542
GluI GCNGC 2 cut(s) 191, 378
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 5 cut(s) 87, 251, 526, 678, 701
HapII CCGG 2 cut(s) 84, 405
HgaI GACGC 1 cut(s) 400
HhaI GCGC 2 cut(s) 195, 543
Hin1II CATG 6 cut(s) 136, 166, 467, 490, 503, 662
Hin6I GCGC 2 cut(s) 193, 541
HinP1I GCGC 2 cut(s) 193, 541
HincII GTYRAC 1 cut(s) 587
HindII GTYRAC 1 cut(s) 587
HinfI GANTC 5 cut(s) 39, 113, 259, 362, 535
HpaII CCGG 2 cut(s) 84, 405
Hpy166II GTNNAC 2 cut(s) 563, 587
Hpy188III TCNNGA 5 cut(s) 155, 384, 386, 451, 506
Hpy8I GTNNAC 2 cut(s) 563, 587
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 4 cut(s) 58, 174, 365, 473
HpyCH4V TGCA 3 cut(s) 190, 281, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 453, 569
HpyF3I CTNAG 1 cut(s) 339
Hsp92II CATG 6 cut(s) 136, 166, 467, 490, 503, 662
HspAI GCGC 2 cut(s) 193, 541
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 3 cut(s) 110, 444, 508
Lsp1109I GCAGC 2 cut(s) 202, 389
LweI GCATC 2 cut(s) 318, 457
MaeIII GTNAC 2 cut(s) 127, 482
MboII GAAGA 5 cut(s) 32, 89, 325, 544, 674
MhlI GDGCHC 1 cut(s) 565
MlsI TGGCCA 2 cut(s) 251, 526
MluCI AATT 4 cut(s) 58, 353, 431, 671
MluNI TGGCCA 2 cut(s) 251, 526
MlyI GAGTC 1 cut(s) 268
MnlI CCTC 5 cut(s) 338, 391, 391, 407, 690
Mox20I TGGCCA 2 cut(s) 251, 526
MscI TGGCCA 2 cut(s) 251, 526
MseI TTAA 2 cut(s) 57, 687
Msp20I TGGCCA 2 cut(s) 251, 526
MspI CCGG 2 cut(s) 84, 405
MspR9I CCNGG 2 cut(s) 84, 406
MwoI GCNNNNNNNGC 3 cut(s) 190, 453, 569
NciI CCSGG 2 cut(s) 84, 406
NcoI CCATGG 1 cut(s) 132
NlaIII CATG 6 cut(s) 136, 166, 467, 490, 503, 662
NlaIV GGNNCC 2 cut(s) 185, 418
NspI RCATGY 2 cut(s) 490, 503
PaeR7I CTCGAG 1 cut(s) 384
PaqCI CACCTGC 1 cut(s) 195
PciI ACATGT 2 cut(s) 486, 499
PciSI GCTCTTC 1 cut(s) 338
PfeI GAWTC 4 cut(s) 39, 113, 362, 535
PkrI GCNGC 2 cut(s) 192, 379
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
PscI ACATGT 2 cut(s) 486, 499
PspN4I GGNNCC 2 cut(s) 185, 418
PspPI GGNCC 2 cut(s) 677, 699
PstI CTGCAG 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 2 cut(s) 57, 687
SatI GCNGC 2 cut(s) 191, 378
Sau96I GGNCC 2 cut(s) 677, 699
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 84, 406
SduI GDGCHC 1 cut(s) 565
SetI ASST 6 cut(s) 107, 142, 189, 208, 449, 484
SfaNI GCATC 2 cut(s) 318, 457
SfcI CTRYAG 1 cut(s) 188
Sfr274I CTCGAG 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 153, 384
SmoI CTYRAG 2 cut(s) 153, 384
Sse9I AATT 4 cut(s) 58, 353, 431, 671
StyD4I CCNGG 2 cut(s) 82, 404
StyI CCWWGG 4 cut(s) 15, 132, 366, 609
TaqI TCGA 1 cut(s) 385
TasI AATT 4 cut(s) 58, 353, 431, 671
TfiI GAWTC 4 cut(s) 39, 113, 362, 535
Tru1I TTAA 2 cut(s) 57, 687
Tru9I TTAA 2 cut(s) 57, 687
TscAI CASTG 1 cut(s) 596
TseI GCWGC 2 cut(s) 190, 377
TspDTI ATGAA 4 cut(s) 375, 492, 614, 675
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 561
XagI CCTNNNNNAGG 1 cut(s) 342
XceI RCATGY 2 cut(s) 490, 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.