RLG00000022859

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013600
Physical Location & Seq
Forward (+)
13435112 .. 13436306
1195 bp
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UTR
Exon/CDS
Intron
RLM00000022859

Sequence Viewer

Length: 567 bp
ATGGTAGCTATAGTATCTCAAGAAAACATGGTTTCTCGCACAGGAAGGCACTTGCAGCGCTACAGCAAAGGTCGTCGCCAAGTCGTAGGATGTATTCCATATAGATATAAAAATTCCAAGCGGAGTCTCTCAAAGGATGATGATGCAAAAGAACTAGAAGTTCTTGTCATTACTTCACAAAAGGGCCAAGGGAGGCTCTTCCCAAAGGGAGGGTGGGAATTAGATGAATCCAAGGAAGGGGCAGCCTCTCGAGAGACGCTGGAGGAAGCTGGGGTACGAGGAACTATTGAGTGTGAATTGGGTAAGTGGAATTTCAAGAGCAAATCCCATGATACTTACTACGTAGGTTACATGTTCCCATTACACGTTCAGGAGCAGTTAGATTTCTGGCCGGAGAAGAACCTGCGTGAAAGATTTTGGATGAGTGTTCAAGAAGCAAGAGACGCTTGTCAGCATTGGTGGATGAAAGAAGCCTTAGATACATTAGTAAGTCGTCTCACATCTCAACAACAACATGAAGACCAAGAATTAGGCCCTGTTCTTAAACCAATGAGGGCAAATCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

189

Amino Acids

21.92

Weight (kDa)

8.38

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 51 - 160 4.9e-10 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 411
AciI CCGC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 2 cut(s) 112, 310
AfaI GTAC 1 cut(s) 276
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 209, 237
AflIII ACRYGT 2 cut(s) 351, 364
AgsI TTSAA 2 cut(s) 316, 431
AluBI AGCT 2 cut(s) 8, 269
AluI AGCT 2 cut(s) 8, 269
Alw26I GTCTC 4 cut(s) 131, 248, 435, 500
Ama87I CYCGRG 1 cut(s) 249
Aor51HI AGCGCT 1 cut(s) 59
AoxI GGCC 3 cut(s) 184, 389, 532
ApeKI GCWGC 2 cut(s) 55, 242
ApoI RAATTY 2 cut(s) 112, 310
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 2 cut(s) 184, 533
AvaI CYCGRG 1 cut(s) 249
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 67, 254
BcoDI GTCTC 4 cut(s) 131, 248, 435, 500
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 2 cut(s) 9, 61
BfoI RGCGCY 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 411
BisI GCNGC 2 cut(s) 56, 243
BlsI GCNGC 2 cut(s) 57, 244
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 184, 533
BmsI GCATC 1 cut(s) 133
BoxI GACNNNNGTC 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 187, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 237
BseDI CCNNGG 2 cut(s) 187, 231
BseGI GGATG 4 cut(s) 95, 142, 426, 468
BseLI CCNNNNNNNGG 2 cut(s) 209, 237
BseXI GCAGC 2 cut(s) 67, 254
BseYI CCCAGC 1 cut(s) 269
BshFI GGCC 3 cut(s) 186, 391, 534
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 209, 237
BsmAI GTCTC 4 cut(s) 131, 248, 435, 500
BsmBI CGTCTC 3 cut(s) 248, 435, 500
BsnI GGCC 3 cut(s) 186, 391, 534
BsoBI CYCGRG 1 cut(s) 249
BspACI CCGC 1 cut(s) 121
BspANI GGCC 3 cut(s) 186, 391, 534
BspMI ACCTGC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 187, 231
BssT1I CCWWGG 2 cut(s) 187, 231
Bst6I CTCTTC 1 cut(s) 203
BstBAI YACGTR 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 475
BstF5I GGATG 4 cut(s) 95, 142, 426, 468
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstMAI GTCTC 4 cut(s) 131, 248, 435, 500
BstMWI GCNNNNNNNGC 2 cut(s) 55, 443
BstNSI RCATGY 1 cut(s) 355
BstPAI GACNNNNGTC 1 cut(s) 447
BstSFI CTRYAG 2 cut(s) 9, 61
BstSNI TACGTA 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 67, 254
BstV2I GAAGAC 1 cut(s) 525
BsuRI GGCC 3 cut(s) 186, 391, 534
BtsCI GGATG 4 cut(s) 95, 142, 426, 468
BveI ACCTGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 2 cut(s) 184, 533
CseI GACGC 2 cut(s) 265, 452
Csp6I GTAC 1 cut(s) 275
CviAII CATG 4 cut(s) 28, 329, 352, 515
CviJI RGCY 8 cut(s) 8, 186, 196, 245, 269, 391, 473, 534
CviKI_1 RGCY 8 cut(s) 8, 186, 196, 245, 269, 391, 473, 534
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco105I TACGTA 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 187, 231
Eco47III AGCGCT 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 533
EcoT14I CCWWGG 2 cut(s) 187, 231
ErhI CCWWGG 2 cut(s) 187, 231
Esp3I CGTCTC 3 cut(s) 248, 435, 500
FaeI CATG 4 cut(s) 31, 332, 355, 518
FaiI YATR 8 cut(s) 11, 29, 100, 102, 108, 330, 353, 516
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FatI CATG 4 cut(s) 27, 328, 351, 514
Fnu4HI GCNGC 2 cut(s) 56, 243
FokI GGATG 4 cut(s) 102, 149, 433, 475
Fsp4HI GCNGC 2 cut(s) 56, 243
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 59
GluI GCNGC 2 cut(s) 56, 243
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 3 cut(s) 186, 391, 534
HapII CCGG 1 cut(s) 392
HgaI GACGC 2 cut(s) 265, 452
HhaI GCGC 1 cut(s) 60
Hin1II CATG 4 cut(s) 31, 332, 355, 518
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 2 cut(s) 124, 227
HpaII CCGG 1 cut(s) 392
Hpy188III TCNNGA 6 cut(s) 20, 249, 251, 316, 371, 431
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 2 cut(s) 39, 230
HpyCH4IV ACGT 2 cut(s) 342, 366
HpyCH4V TGCA 2 cut(s) 55, 146
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 443
HpyF3I CTNAG 1 cut(s) 475
HpySE526I ACGT 2 cut(s) 342, 366
Hsp92II CATG 4 cut(s) 31, 332, 355, 518
HspAI GCGC 1 cut(s) 58
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 8 cut(s) 27, 245, 255, 356, 373, 405, 416, 549
Lsp1109I GCAGC 2 cut(s) 67, 254
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 155
MaeII ACGT 2 cut(s) 342, 366
MaeIII GTNAC 1 cut(s) 347
MboII GAAGA 3 cut(s) 190, 409, 530
MluCI AATT 5 cut(s) 112, 218, 296, 310, 527
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 6 cut(s) 186, 203, 256, 256, 272, 546
MseI TTAA 1 cut(s) 543
MspI CCGG 1 cut(s) 392
MwoI GCNNNNNNNGC 2 cut(s) 55, 443
NlaIII CATG 4 cut(s) 31, 332, 355, 518
NspI RCATGY 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 249
PciI ACATGT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 57, 244
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
Ppu21I YACGTR 1 cut(s) 343
PscI ACATGT 1 cut(s) 351
PshAI GACNNNNGTC 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 269
PspPI GGNCC 2 cut(s) 184, 533
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 2 cut(s) 56, 243
Sau96I GGNCC 2 cut(s) 184, 533
SchI GAGTC 1 cut(s) 133
SetI ASST 7 cut(s) 10, 73, 271, 345, 349, 369, 405
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 2 cut(s) 9, 61
Sfr274I CTCGAG 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 18, 249
SmoI CTYRAG 2 cut(s) 18, 249
SnaBI TACGTA 1 cut(s) 343
Sse9I AATT 5 cut(s) 112, 218, 296, 310, 527
SsiI CCGC 1 cut(s) 121
SspMI CTAG 1 cut(s) 155
StyI CCWWGG 2 cut(s) 187, 231
TaiI ACGT 2 cut(s) 345, 369
TaqI TCGA 1 cut(s) 250
TasI AATT 5 cut(s) 112, 218, 296, 310, 527
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TseI GCWGC 2 cut(s) 55, 242
TspDTI ATGAA 3 cut(s) 240, 479, 531
XapI RAATTY 2 cut(s) 112, 310
XceI RCATGY 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XhoI CTCGAG 1 cut(s) 249
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.