MD09G1270800.v1.1

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013600
Physical Location & Seq
Forward (+)
34517774 .. 34519337
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1270800.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGTAGCTTTGATACCTCAAGAAAACACGGTTGCTGCTTTGGTATCTCGCACTGGAAGGCATATGCAGCGTTACAGCAAAGGTCGTCGCCAAGTTGTCGGATGTATTCCATATAGATATAAAGCTTCCAAGCAAACTTCCTTAAAGGATGATGCACAAGAACTTGAAGTCCTTGTCATTAGTTCACAGAAGGGCAAGGGGATGCTCTTTCCAAAGGGAGGGTGGGAACAGGACGAATCCAAGGAAGGGGCAGCGTCCCGAGAGACGCTAGAGGAAGCAGGAGTACGAGGACTCATTGGGTGTGAATTGGGTGACTGGAGTTTCAAGAGCAAAAGCCATGATTCTTTCTATGAAGGCTACATGTTCCCTTTACTTGTTCATGAGCAGTTAGATTTCTGGCCAGAAAAGAACCTCAGGCAAAGAATTTGGATGAATGTTGAAGAAGCAAGAGAAGTCTGTCAGCATTGGTGGATGAAAGAAGCTTTGGATAGATTAGTAAATCGACTAACAACTCAGCAACAAAAGCATCAAGGCCCTCTTCTTGAATCTAGGACGGAAAATCTGTTGGCATGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

193

Amino Acids

22.18

Weight (kDa)

7.69

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 54 - 163 1.2e-09 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 218, 246
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 4 cut(s) 167, 325, 440, 545
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
AluBI AGCT 3 cut(s) 8, 125, 482
AluI AGCT 3 cut(s) 8, 125, 482
Alw26I GTCTC 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 2 cut(s) 398, 532
ApeKI GCWGC 3 cut(s) 35, 67, 251
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 258
AxyI CCTNAGG 1 cut(s) 413
BalI TGGCCA 1 cut(s) 400
BarI GAAGNNNNNNTAC 2 cut(s) 267, 299
BbvI GCAGC 3 cut(s) 22, 79, 263
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 2 cut(s) 269, 549
BisI GCNGC 3 cut(s) 36, 68, 252
BlsI GCNGC 3 cut(s) 37, 69, 253
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 533
BmsI GCATC 3 cut(s) 142, 192, 535
BpmI CTGGAG 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 246
Bse1I ACTGG 2 cut(s) 58, 320
Bse21I CCTNAGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 5 cut(s) 107, 154, 207, 435, 477
BseLI CCNNNNNNNGG 2 cut(s) 218, 246
BseMII CTCAG 2 cut(s) 427, 527
BseNI ACTGG 2 cut(s) 58, 320
BseXI GCAGC 3 cut(s) 22, 79, 263
BshFI GGCC 2 cut(s) 400, 534
BsiHKCI CYCGRG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 2 cut(s) 218, 246
BsmAI GTCTC 1 cut(s) 257
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 2 cut(s) 400, 534
BsoBI CYCGRG 1 cut(s) 258
BspANI GGCC 2 cut(s) 400, 534
BspCNI CTCAG 2 cut(s) 426, 526
BspHI TCATGA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 58, 320
BssECI CCNNGG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 543
BstDEI CTNAG 2 cut(s) 413, 513
BstF5I GGATG 5 cut(s) 107, 154, 207, 435, 477
BstMAI GTCTC 1 cut(s) 257
BstMWI GCNNNNNNNGC 2 cut(s) 67, 523
BstNSI RCATGY 2 cut(s) 364, 573
BstV1I GCAGC 3 cut(s) 22, 79, 263
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 2 cut(s) 400, 534
BtsCI GGATG 5 cut(s) 107, 154, 207, 435, 477
BtsIMutI CAGTG 1 cut(s) 51
CciI TCATGA 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 533
CseI GACGC 2 cut(s) 243, 274
Csp6I GTAC 1 cut(s) 284
CviAII CATG 4 cut(s) 338, 361, 380, 570
CviJI RGCY 7 cut(s) 8, 125, 336, 357, 400, 482, 534
CviKI_1 RGCY 7 cut(s) 8, 125, 336, 357, 400, 482, 534
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 2 cut(s) 413, 513
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 413
Eco88I CYCGRG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 4 cut(s) 341, 364, 383, 573
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 1 cut(s) 241
FatI CATG 4 cut(s) 337, 360, 379, 569
FauNDI CATATG 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 36, 68, 252
FokI GGATG 5 cut(s) 114, 161, 214, 442, 484
Fsp4HI GCNGC 3 cut(s) 36, 68, 252
FspBI CTAG 2 cut(s) 269, 549
GluI GCNGC 3 cut(s) 36, 68, 252
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 2 cut(s) 400, 534
HgaI GACGC 2 cut(s) 243, 274
Hin1II CATG 4 cut(s) 341, 364, 383, 573
HindIII AAGCTT 2 cut(s) 123, 480
HinfI GANTC 4 cut(s) 236, 291, 341, 545
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 5 cut(s) 20, 258, 325, 380, 542
Hpy8I GTNNAC 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 4 cut(s) 51, 184, 239, 347
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 67, 155
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 523
HpyF3I CTNAG 2 cut(s) 413, 513
Hsp92II CATG 4 cut(s) 341, 364, 383, 573
LpnPI CCDG 7 cut(s) 39, 215, 264, 301, 382, 400, 414
Lsp1109I GCAGC 3 cut(s) 22, 79, 263
LweI GCATC 3 cut(s) 142, 192, 535
MaeI CTAG 2 cut(s) 269, 549
MaeIII GTNAC 2 cut(s) 71, 311
MboII GAAGA 2 cut(s) 452, 530
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 2 cut(s) 305, 423
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 6 cut(s) 27, 212, 265, 281, 422, 546
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 1 cut(s) 143
Msp20I TGGCCA 1 cut(s) 400
MwoI GCNNNNNNNGC 2 cut(s) 67, 523
NdeI CATATG 1 cut(s) 63
NlaIII CATG 4 cut(s) 341, 364, 383, 573
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 2 cut(s) 364, 573
PagI TCATGA 1 cut(s) 379
PciI ACATGT 1 cut(s) 360
PfeI GAWTC 3 cut(s) 236, 341, 545
PkrI GCNGC 3 cut(s) 37, 69, 253
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PscI ACATGT 1 cut(s) 360
PspPI GGNCC 1 cut(s) 533
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 3 cut(s) 36, 68, 252
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 1 cut(s) 285
SetI ASST 6 cut(s) 10, 19, 85, 127, 414, 484
SfaNI GCATC 3 cut(s) 142, 192, 535
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 305, 423
SspMI CTAG 2 cut(s) 269, 549
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 502
TasI AATT 2 cut(s) 305, 423
TfiI GAWTC 3 cut(s) 236, 341, 545
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 3 cut(s) 35, 67, 251
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 366, 368, 446, 488
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 1 cut(s) 58
XapI RAATTY 1 cut(s) 423
XceI RCATGY 2 cut(s) 364, 573
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XspI CTAG 2 cut(s) 269, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.