MD17G1269000.v1.1

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013600
Physical Location & Seq
Forward (+)
32909109 .. 32910498
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1269000.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGTAGCTTTGATACCCCAAGAAAACATGGTTGCTGCTTTGGTATCTCGCACTGGAAGGCATATGCAGCGTTACAGCAAAGGTCATCGCCAAGTTGTAGGATGTATTCCGTATAGATATAAAGCTTCCAAGCAAACTCCCTTTAAGAATGATGCAAAAGAACTTGAAGTTCTTGTCATTAGCTCGCAGAAGGGAAAGGGGATGCTCTTTCCAAAGGGAGGATGGGAACTAGACGAATCCAAGGAAGGGGCAGCGGCACGAGAGACGCTAGAGGAAGCAGGAGTACGGGGACTCATTGAGCGTGAATTGGGTGAATGGAGTTTCAATAGCAAAAGCCATGATTCTTACAAAGAAGGCTACATGTTCCCTTTACTTGTTCAGGAGCAGTTGGATTTCTGGCCGGAGAAGAACCTCAGGCAAAGAATTTGGATGAGTGTTGAAGATGCAAGAGAAGTTTGTCAGCATTGGTGGATGAAAGAAGCCTTGGACAGATTAGTAAATCGACTAACAACTCAGCAACAAAAGGATCAAGGCCCTCTTCTTAAATCTAGGAAGGAAAATCTGATCGCATGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

193

Amino Acids

22.27

Weight (kDa)

8.93

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 54 - 163 1.4e-10 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 254
AclWI GGATC 1 cut(s) 534
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 218, 246
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 3 cut(s) 167, 325, 440
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 3 cut(s) 8, 125, 183
AluI AGCT 3 cut(s) 8, 125, 183
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 534
AoxI GGCC 2 cut(s) 398, 532
ApeKI GCWGC 3 cut(s) 35, 67, 251
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 267, 299
BauI CACGAG 1 cut(s) 258
BbvI GCAGC 3 cut(s) 22, 79, 263
BccI CCATC 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 3 cut(s) 230, 269, 549
BisI GCNGC 4 cut(s) 36, 68, 252, 255
BlsI GCNGC 4 cut(s) 37, 69, 253, 256
BmgT120I GGNCC 1 cut(s) 533
BmsI GCATC 3 cut(s) 142, 192, 433
BsaJI CCNNGG 2 cut(s) 240, 483
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 246
Bse1I ACTGG 1 cut(s) 58
Bse21I CCTNAGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 240, 483
BseGI GGATG 5 cut(s) 107, 207, 227, 435, 477
BseLI CCNNNNNNNGG 2 cut(s) 218, 246
BseMII CTCAG 2 cut(s) 427, 527
BseNI ACTGG 1 cut(s) 58
BseXI GCAGC 3 cut(s) 22, 79, 263
BshFI GGCC 2 cut(s) 400, 534
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 218, 246
BsmAI GTCTC 1 cut(s) 257
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 2 cut(s) 400, 534
Bsp143I GATC 2 cut(s) 526, 564
BspACI CCGC 1 cut(s) 254
BspANI GGCC 2 cut(s) 400, 534
BspCNI CTCAG 2 cut(s) 426, 526
BspPI GGATC 1 cut(s) 534
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 240, 483
BssMI GATC 2 cut(s) 526, 564
BssSI CACGAG 1 cut(s) 258
BssT1I CCWWGG 2 cut(s) 240, 483
Bst2BI CACGAG 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 543
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 413, 513
BstF5I GGATG 5 cut(s) 107, 207, 227, 435, 477
BstKTI GATC 2 cut(s) 529, 567
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 2 cut(s) 526, 564
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNSI RCATGY 2 cut(s) 364, 573
BstV1I GCAGC 3 cut(s) 22, 79, 263
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 2 cut(s) 400, 534
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 5 cut(s) 107, 207, 227, 435, 477
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 533
CseI GACGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 284
CviAII CATG 4 cut(s) 28, 338, 361, 570
CviJI RGCY 8 cut(s) 8, 125, 183, 336, 357, 400, 482, 534
CviKI_1 RGCY 8 cut(s) 8, 125, 183, 336, 357, 400, 482, 534
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 2 cut(s) 413, 513
DpnI GATC 2 cut(s) 528, 566
DpnII GATC 2 cut(s) 526, 564
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco130I CCWWGG 2 cut(s) 240, 483
Eco81I CCTNAGG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 533
EcoT14I CCWWGG 2 cut(s) 240, 483
ErhI CCWWGG 2 cut(s) 240, 483
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 4 cut(s) 31, 341, 364, 573
FaiI YATR 9 cut(s) 29, 63, 65, 114, 120, 339, 362, 571, 577
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 1 cut(s) 303
FatI CATG 4 cut(s) 27, 337, 360, 569
FauNDI CATATG 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 36, 68, 252, 255
FokI GGATG 5 cut(s) 114, 214, 234, 442, 484
Fsp4HI GCNGC 4 cut(s) 36, 68, 252, 255
FspBI CTAG 3 cut(s) 230, 269, 549
GluI GCNGC 4 cut(s) 36, 68, 252, 255
HaeIII GGCC 2 cut(s) 400, 534
HapII CCGG 1 cut(s) 401
HgaI GACGC 1 cut(s) 274
Hin1II CATG 4 cut(s) 31, 341, 364, 573
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 3 cut(s) 236, 291, 341
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 564
Hpy188III TCNNGA 1 cut(s) 380
HpyAV CCTTC 5 cut(s) 51, 184, 239, 347, 547
HpyCH4V TGCA 3 cut(s) 67, 155, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 2 cut(s) 413, 513
Hsp92II CATG 4 cut(s) 31, 341, 364, 573
Kzo9I GATC 2 cut(s) 526, 564
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 6 cut(s) 39, 264, 365, 382, 400, 414
Lsp1109I GCAGC 3 cut(s) 22, 79, 263
LweI GCATC 3 cut(s) 142, 192, 433
MaeI CTAG 3 cut(s) 230, 269, 549
MaeIII GTNAC 1 cut(s) 71
MalI GATC 2 cut(s) 528, 566
MboI GATC 2 cut(s) 526, 564
MboII GAAGA 3 cut(s) 418, 452, 530
MluCI AATT 2 cut(s) 305, 423
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 4 cut(s) 212, 265, 422, 546
MseI TTAA 2 cut(s) 144, 543
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 67
NdeI CATATG 1 cut(s) 63
NdeII GATC 2 cut(s) 526, 564
NlaIII CATG 4 cut(s) 31, 341, 364, 573
NspI RCATGY 2 cut(s) 364, 573
PciI ACATGT 1 cut(s) 360
PfeI GAWTC 2 cut(s) 236, 341
PkrI GCNGC 4 cut(s) 37, 69, 253, 256
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PscI ACATGT 1 cut(s) 360
PspPI GGNCC 1 cut(s) 533
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 144, 543
SatI GCNGC 4 cut(s) 36, 68, 252, 255
Sau3AI GATC 2 cut(s) 526, 564
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 1 cut(s) 285
SetI ASST 5 cut(s) 10, 85, 127, 185, 414
SfaNI GCATC 3 cut(s) 142, 192, 433
Sse9I AATT 2 cut(s) 305, 423
SsiI CCGC 1 cut(s) 254
SspMI CTAG 3 cut(s) 230, 269, 549
StyI CCWWGG 2 cut(s) 240, 483
TaqI TCGA 1 cut(s) 502
TasI AATT 2 cut(s) 305, 423
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 236, 341
Tru1I TTAA 2 cut(s) 144, 543
Tru9I TTAA 2 cut(s) 144, 543
TscAI CASTG 1 cut(s) 58
TseI GCWGC 3 cut(s) 35, 67, 251
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 58
XapI RAATTY 1 cut(s) 423
XceI RCATGY 2 cut(s) 364, 573
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XspI CTAG 3 cut(s) 230, 269, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.