FvH4_1g30420

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006297
Physical Location & Seq
Forward (+)
24116804 .. 24118472
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30420.t1

Sequence Viewer

Length: 954 bp
ATGAAGCTCGCAGGCCTGAAATCAATCGAGGACGCTCACGACGAGTCGGTATGGGCGGTCACCTGGGTCCCCGCCACCGATGCCCGACCCGCTTTGCTGTTAACTGGGTCACTCGACGAGTCCGTTCGCCTCTGGCAACCCGACGAGCTCGTCAACCGGGGAAAGATCACCGGTCACTGCCTCGGCGTCGCTTCCGTCGCCGCTCACCCTTCCGGTTACATCGCCGCCTCGGCTTCGCTCGATAGCTTCGTCAGGATCTTCGACGTTGATTCCAACTCTACCATCGCCAGCTTCGAGGCGCCGCCGTCGGAAGTCTGGCAGTTACGATTCAATCCCCAGGGTACCATGCTAGCTGCGGCCGGTGGTGGTAGTGCATCAGTCAAGGTCTGGGACACCAGCACATGGAACCTGGTTGCCACATTGTTGATTCCTCGCCCAGAAGGAGCCAAGCCTGCTGACAAAAGCAGCAGCAAGAAGTTTGTGCTATCAGTTGCATGGAGTCCTGATGGGAGACGCATAGCTTGTGGCTCCATGGATGGAACTGTTTCTGTTTTCGACACAGATCGTGGCAAGTTTCTGCACCATCTGGAAGGCCACTCCATGCCCGTAAGGTCTCTTGTGTTTTCCCCTGTTGAACCACGGTTACTCTTCACTGGCTCGGATGATCAACATGTGCACATGTATGATGCTGAGGGGAAAACTCAGGTTGGGTCCATGTCAGGGCATAACGGTTGGGTCTTGAGTGTGGATGCAAGCACAGATGGGGCATCCCTAGCAACCGGCTCAAGTGATAGGACTGTGAAACTGTGGGATATTGGCATGAAGGCGGCTGTGCAGACAATGAGCAACCATAATGACCAAGTCTGGGGAGTGGCCTTTCGACCTGGAGGAATGCCTGGTAGACTTGCTAGTGTCTCTGATGACAAGAGCATCTCACTGTATGACTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000428 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034968 GO:0035327 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0055087 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
WD40

Protein Analysis

318

Amino Acids

33.87

Weight (kDa)

5.65

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 7 - 88 4e-06 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 10 - 145 3.7e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 12 - 131 6e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 12 - 141 1.5e-19 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 13 - 138 1.4e-17 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 13 - 141 4.8e-17 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 13 - 140 2.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 25 - 133 4.7e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 31 - 132 6.8e-06 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 31 - 218 7.2e-20 WDHD1 first WD40 domain
Beta-prop_EML PF23409 41 - 233 2.2e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR19_1st PF23389 71 - 234 1.9e-08 WDR19 first beta-propeller
Beta-prop_WDR3_2nd PF25172 85 - 315 1e-26 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 105 - 235 4.7e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 122 - 315 2.1e-35 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 151 - 289 6.5e-19 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 154 - 315 4.3e-26 CDC20/Fizzy WD40 domain
ANAPC4_WD40 PF12894 154 - 206 1e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 154 - 235 1.2e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 155 - 233 2.7e-06 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 156 - 209 2.6e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 157 - 316 5.3e-34 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 158 - 270 1.8e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 158 - 295 2.5e-15 WDR55
Beta-prop_RIG_2nd PF23775 158 - 231 1.9e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 158 - 242 1.6e-19 THOC3 beta-propeller domain
WD40_Gbeta PF25391 159 - 316 5.8e-16 G protein beta WD-40 repeat protein
WD40 PF00400 159 - 186 2.3e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_1st PF25171 173 - 297 1.3e-09 WDR36/Utp21 first beta-propeller
WD40 PF00400 190 - 229 2.8e-06 WD domain, G-beta repeat
EIF3I PF24805 196 - 295 7.7e-12 EIF3I
WD40_Prp19 PF24814 211 - 316 9.7e-16 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 215 - 297 5.6e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 215 - 282 1e-09 WDHD1 first WD40 domain
WD40 PF00400 234 - 271 3.2e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 238 - 316 1.8e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 239 - 316 6.1e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 242 - 316 5.4e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 298, 341
AccB7I CCANNNNNTGG 1 cut(s) 402
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 901
AciI CCGC 8 cut(s) 56, 72, 90, 201, 225, 302, 356, 827
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 357
AcyI GRCGYC 2 cut(s) 186, 299
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 3 cut(s) 402, 720, 865
AflIII ACRYGT 2 cut(s) 670, 678
AgeI ACCGGT 1 cut(s) 170
AgsI TTSAA 2 cut(s) 331, 635
AjnI CCWGG 5 cut(s) 62, 336, 408, 883, 895
AluBI AGCT 6 cut(s) 7, 148, 246, 291, 353, 521
AluI AGCT 6 cut(s) 7, 148, 246, 291, 353, 521
Alw21I GWGCWC 2 cut(s) 150, 678
Alw26I GTCTC 3 cut(s) 505, 618, 919
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 1 cut(s) 263
AoxI GGCC 4 cut(s) 13, 357, 592, 873
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 353, 465, 468
AsiGI ACCGGT 1 cut(s) 170
Asp700I GAANNNNTTC 1 cut(s) 544
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 67, 711
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 52, 160, 197
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 2 cut(s) 67, 711
BaeGI GKGCMC 1 cut(s) 678
BanI GGYRCC 2 cut(s) 298, 341
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 150, 678
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 340, 477, 480
BccI CCATC 5 cut(s) 290, 500, 530, 591, 755
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 5 cut(s) 64, 338, 410, 885, 897
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 3 cut(s) 505, 618, 919
BfaI CTAG 3 cut(s) 350, 773, 909
BfoI RGCGCY 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 8 cut(s) 201, 225, 302, 354, 357, 466, 469, 828
BlsI GCNGC 8 cut(s) 202, 226, 303, 355, 358, 467, 470, 829
Bme1390I CCNGG 6 cut(s) 64, 158, 338, 410, 885, 897
Bme18I GGWCC 2 cut(s) 67, 711
BmgT120I GGNCC 2 cut(s) 67, 711
BmiI GGNNCC 8 cut(s) 68, 69, 300, 343, 407, 445, 529, 712
BmrFI CCNGG 6 cut(s) 64, 158, 338, 410, 885, 897
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 6 cut(s) 70, 383, 676, 739, 776, 939
BmtI GCTAGC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 1 cut(s) 690
BpuEI CTTGAG 2 cut(s) 760, 769
BpuMI CCSGG 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 186, 299
BsaI GGTCTC 1 cut(s) 618
BsaJI CCNNGG 8 cut(s) 63, 157, 181, 228, 336, 337, 531, 638
BsaWI WCCGGW 2 cut(s) 170, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 402, 720, 865
Bse118I RCCGGY 3 cut(s) 170, 359, 779
Bse1I ACTGG 2 cut(s) 109, 658
BseBI CCWGG 5 cut(s) 64, 338, 410, 885, 897
BseDI CCNNGG 8 cut(s) 63, 157, 181, 228, 336, 337, 531, 638
BseGI GGATG 4 cut(s) 541, 667, 754, 767
BseLI CCNNNNNNNGG 3 cut(s) 402, 720, 865
BseMII CTCAG 2 cut(s) 681, 716
BseNI ACTGG 2 cut(s) 109, 658
BseSI GKGCMC 1 cut(s) 678
BseX3I CGGCCG 1 cut(s) 357
BseXI GCAGC 3 cut(s) 340, 477, 480
BsgI GTGCAG 2 cut(s) 563, 854
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 4 cut(s) 15, 359, 594, 875
BshNI GGYRCC 2 cut(s) 298, 341
BshTI ACCGGT 1 cut(s) 170
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 150, 678
BsiSI CCGG 5 cut(s) 157, 171, 213, 360, 780
BslFI GGGAC 2 cut(s) 53, 404
BslI CCNNNNNNNGG 3 cut(s) 402, 720, 865
BsmAI GTCTC 3 cut(s) 505, 618, 919
BsmBI CGTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 53, 404
BsmI GAATGC 1 cut(s) 897
BsnI GGCC 4 cut(s) 15, 359, 594, 875
Bso31I GGTCTC 1 cut(s) 618
Bsp1286I GDGCHC 2 cut(s) 150, 678
Bsp143I GATC 4 cut(s) 165, 255, 562, 664
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 8 cut(s) 56, 72, 90, 201, 225, 302, 356, 827
BspANI GGCC 4 cut(s) 15, 359, 594, 875
BspCNI CTCAG 2 cut(s) 682, 715
BspLI GGNNCC 8 cut(s) 68, 69, 300, 343, 407, 445, 529, 712
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 1 cut(s) 263
BspT107I GGYRCC 2 cut(s) 298, 341
BspTNI GGTCTC 1 cut(s) 618
BsrBI CCGCTC 1 cut(s) 203
BsrFI RCCGGY 3 cut(s) 170, 359, 779
BsrI ACTGG 2 cut(s) 109, 658
BssAI RCCGGY 3 cut(s) 170, 359, 779
BssECI CCNNGG 8 cut(s) 63, 157, 181, 228, 336, 337, 531, 638
BssMI GATC 4 cut(s) 165, 255, 562, 664
BssNI GRCGYC 2 cut(s) 186, 299
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 5 cut(s) 64, 338, 410, 885, 897
Bst4CI ACNGT 6 cut(s) 544, 642, 731, 799, 807, 939
Bst6I CTCTTC 1 cut(s) 653
BstACI GRCGYC 2 cut(s) 186, 299
BstC8I GCNNGC 6 cut(s) 9, 13, 289, 351, 453, 754
BstDEI CTNAG 2 cut(s) 690, 702
BstDSI CCRYGG 2 cut(s) 531, 638
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 4 cut(s) 541, 667, 754, 767
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 4 cut(s) 168, 258, 565, 667
BstMAI GTCTC 3 cut(s) 505, 618, 919
BstMBI GATC 4 cut(s) 165, 255, 562, 664
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 6 cut(s) 80, 89, 197, 230, 452, 773
BstNI CCWGG 5 cut(s) 64, 338, 410, 885, 897
BstNSI RCATGY 2 cut(s) 674, 682
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 6 cut(s) 62, 156, 336, 408, 883, 895
BstSLI GKGCMC 1 cut(s) 678
BstV1I GCAGC 3 cut(s) 340, 477, 480
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 357
BsuRI GGCC 4 cut(s) 15, 359, 594, 875
BtgI CCRYGG 2 cut(s) 531, 638
BtgZI GCGATG 2 cut(s) 205, 268
BtsCI GGATG 4 cut(s) 541, 667, 754, 767
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 3 cut(s) 175, 651, 935
Cac8I GCNNGC 6 cut(s) 9, 13, 289, 351, 453, 754
CfoI GCGC 1 cut(s) 301
Cfr10I RCCGGY 3 cut(s) 170, 359, 779
Cfr13I GGNCC 2 cut(s) 67, 711
CseI GACGC 3 cut(s) 41, 175, 522
CsiI ACCWGGT 1 cut(s) 408
Csp6I GTAC 1 cut(s) 342
CspAI ACCGGT 1 cut(s) 170
CviAII CATG 9 cut(s) 346, 402, 495, 532, 601, 671, 679, 715, 820
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 2 cut(s) 690, 702
DinI GGCGCC 1 cut(s) 300
DpnI GATC 4 cut(s) 167, 257, 564, 666
DpnII GATC 4 cut(s) 165, 255, 562, 664
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 357
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 531
Eco147I AGGCCT 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 618
Eco47I GGWCC 2 cut(s) 67, 711
Eco52I CGGCCG 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 148
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 67
EcoO65I GGTNACC 1 cut(s) 58
EcoRII CCWGG 5 cut(s) 62, 336, 408, 883, 895
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 300
EheI GGCGCC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 1 cut(s) 505
FaeI CATG 9 cut(s) 349, 405, 498, 535, 604, 674, 682, 718, 823
FaqI GGGAC 2 cut(s) 53, 404
FatI CATG 9 cut(s) 345, 401, 494, 531, 600, 670, 678, 714, 819
FauI CCCGC 2 cut(s) 79, 97
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 901
Fnu4HI GCNGC 8 cut(s) 201, 225, 302, 354, 357, 466, 469, 828
FokI GGATG 4 cut(s) 548, 674, 754, 761
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 8 cut(s) 201, 225, 302, 354, 357, 466, 469, 828
FspBI CTAG 3 cut(s) 350, 773, 909
GlaI GCGC 1 cut(s) 300
GluI GCNGC 8 cut(s) 201, 225, 302, 354, 357, 466, 469, 828
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 4 cut(s) 15, 359, 594, 875
HapII CCGG 5 cut(s) 157, 171, 213, 360, 780
HgaI GACGC 3 cut(s) 41, 175, 522
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 2 cut(s) 186, 299
Hin1II CATG 9 cut(s) 349, 405, 498, 535, 604, 674, 682, 718, 823
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 2 cut(s) 102, 154
HindII GTYRAC 2 cut(s) 102, 154
HinfI GANTC 6 cut(s) 44, 119, 269, 327, 427, 499
HpaI GTTAAC 1 cut(s) 102
HpaII CCGG 5 cut(s) 157, 171, 213, 360, 780
HphI GGTGA 3 cut(s) 52, 160, 197
Hpy166II GTNNAC 4 cut(s) 102, 154, 676, 902
Hpy188I TCNGA 3 cut(s) 310, 661, 919
Hpy188III TCNNGA 6 cut(s) 38, 253, 503, 587, 739, 951
Hpy8I GTNNAC 4 cut(s) 102, 154, 676, 902
Hpy99I CGWCG 7 cut(s) 44, 119, 146, 191, 200, 266, 310
HpyAV CCTTC 4 cut(s) 219, 434, 584, 817
HpyCH4III ACNGT 6 cut(s) 544, 642, 731, 799, 807, 939
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 6 cut(s) 374, 494, 580, 676, 752, 835
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 89, 197, 230, 452, 773
HpyF3I CTNAG 2 cut(s) 690, 702
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 2 cut(s) 186, 299
Hsp92II CATG 9 cut(s) 349, 405, 498, 535, 604, 674, 682, 718, 823
HspAI GCGC 1 cut(s) 299
KasI GGCGCC 1 cut(s) 298
KflI GGGWCCC 1 cut(s) 67
KpnI GGTACC 1 cut(s) 345
Ksp22I TGATCA 1 cut(s) 664
KspAI GTTAAC 1 cut(s) 102
Kzo9I GATC 4 cut(s) 165, 255, 562, 664
LmnI GCTCC 2 cut(s) 443, 533
Lsp1109I GCAGC 3 cut(s) 340, 477, 480
LweI GCATC 6 cut(s) 70, 383, 676, 739, 776, 939
MabI ACCWGGT 1 cut(s) 408
MaeI CTAG 3 cut(s) 350, 773, 909
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 6 cut(s) 58, 108, 173, 215, 321, 642
MalI GATC 4 cut(s) 167, 257, 564, 666
MbiI CCGCTC 1 cut(s) 203
MboI GATC 4 cut(s) 165, 255, 562, 664
MboII GAAGA 2 cut(s) 250, 640
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 2 cut(s) 150, 678
Mly113I GGCGCC 1 cut(s) 299
MlyI GAGTC 3 cut(s) 53, 128, 508
MmeI TCCRAC 2 cut(s) 288, 297
MnlI CCTC 8 cut(s) 22, 140, 191, 238, 289, 441, 685, 881
MroXI GAANNNNTTC 1 cut(s) 544
MseI TTAA 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 5 cut(s) 157, 171, 213, 360, 780
MspR9I CCNGG 6 cut(s) 64, 158, 338, 410, 885, 897
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 5 cut(s) 64, 338, 410, 885, 897
MwoI GCNNNNNNNGC 6 cut(s) 80, 89, 197, 230, 452, 773
NarI GGCGCC 1 cut(s) 299
NciI CCSGG 1 cut(s) 158
NcoI CCATGG 1 cut(s) 531
NdeII GATC 4 cut(s) 165, 255, 562, 664
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 9 cut(s) 349, 405, 498, 535, 604, 674, 682, 718, 823
NlaIV GGNNCC 8 cut(s) 68, 69, 300, 343, 407, 445, 529, 712
NmeAIII GCCGAG 2 cut(s) 162, 209
NmuCI GTSAC 3 cut(s) 58, 108, 173
NspI RCATGY 2 cut(s) 674, 682
PasI CCCWGGG 1 cut(s) 337
PceI AGGCCT 1 cut(s) 15
PciI ACATGT 2 cut(s) 670, 678
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 120, 246, 291
PctI GAATGC 1 cut(s) 897
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 3 cut(s) 269, 327, 427
PflFI GACNNNGTC 1 cut(s) 860
PflMI CCANNNNNTGG 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 8 cut(s) 202, 226, 303, 355, 358, 467, 470, 829
PleI GAGTC 3 cut(s) 52, 127, 507
PluTI GGCGCC 1 cut(s) 302
PpsI GAGTC 3 cut(s) 52, 127, 507
PpuMI RGGWCCY 1 cut(s) 67
PscI ACATGT 2 cut(s) 670, 678
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 5 cut(s) 62, 336, 408, 883, 895
PspEI GGTNACC 1 cut(s) 58
PspGI CCWGG 5 cut(s) 62, 336, 408, 883, 895
PspN4I GGNNCC 8 cut(s) 68, 69, 300, 343, 407, 445, 529, 712
PspPI GGNCC 2 cut(s) 67, 711
PspPPI RGGWCCY 1 cut(s) 67
PsrI GAACNNNNNNTAC 2 cut(s) 627, 659
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 860
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 8 cut(s) 201, 225, 302, 354, 357, 466, 469, 828
Sau3AI GATC 4 cut(s) 165, 255, 562, 664
Sau96I GGNCC 2 cut(s) 67, 711
SchI GAGTC 3 cut(s) 53, 128, 508
ScrFI CCNGG 6 cut(s) 64, 158, 338, 410, 885, 897
SduI GDGCHC 2 cut(s) 150, 678
SexAI ACCWGGT 1 cut(s) 408
SfaNI GCATC 6 cut(s) 70, 383, 676, 739, 776, 939
SfoI GGCGCC 1 cut(s) 300
SinI GGWCC 2 cut(s) 67, 711
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 2 cut(s) 739, 784
SmoI CTYRAG 2 cut(s) 739, 784
SseBI AGGCCT 1 cut(s) 15
SsiI CCGC 8 cut(s) 56, 72, 90, 201, 225, 302, 356, 827
SspDI GGCGCC 1 cut(s) 298
SspMI CTAG 3 cut(s) 350, 773, 909
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 15
StyD4I CCNGG 6 cut(s) 62, 156, 336, 408, 883, 895
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 6 cut(s) 544, 642, 731, 799, 807, 939
TaiI ACGT 1 cut(s) 267
TaqI TCGA 7 cut(s) 27, 114, 240, 261, 294, 555, 880
TauI GCSGC 5 cut(s) 203, 227, 304, 359, 830
TfiI GAWTC 3 cut(s) 269, 327, 427
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 3 cut(s) 182, 658, 942
TseFI GTSAC 3 cut(s) 58, 108, 173
TseI GCWGC 3 cut(s) 353, 465, 468
Tsp45I GTSAC 3 cut(s) 58, 108, 173
TspDTI ATGAA 2 cut(s) 17, 836
TspGWI ACGGA 2 cut(s) 112, 184
TspRI CASTG 3 cut(s) 182, 658, 942
Tth111I GACNNNGTC 1 cut(s) 860
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 67, 711
XceI RCATGY 2 cut(s) 674, 682
XmiI GTMKAC 1 cut(s) 901
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 3 cut(s) 350, 773, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.