Prupe.6G180900_v2.0.a1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0006297
Physical Location & Seq
Reverse (-)
18758872 .. 18767755
8884 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G180900.8

Sequence Viewer

Length: 954 bp
ATGAAGCTTGCAGGGTTGAAATCAATCGAGGACGCGCATGAGGAGTCAGTATGGGCGGTGACATGGGTCCCGTCAACGGAGACCCGACCTGCTTTGTTGTTAACCGGATCACTGGACGAGACCGTGAGGCTGTGGCAGCCAGACGAGCTAGTCAACCAGGGCAAGATGACCGGCCACTGCCTCGGTGTCGCTTCAGTGGCAGCTCACCCCTCTGGCCATATTGCTGCCTCGGCTTCACTCGACAGCTTTGTTAGGGTTTTCGATGTCGACTCCAACAACACCATCGCTAGTCTCGAAGCCCCCCCCTCTGAAGTCTGGCAATTACGCTTCAATCCTCAGGGTACCATGCTAGCTGTGGCGGGTGGGGGAAGTGCATCAGTCAAAATTTGGGACACCACCACATGGAATCTCTTTGCCACATTGTCAATTCCTCGCCCAGAAGGAACCAAGCCCACGGATAAAAGCAGCAGCAAGAAGTTTGTGCTATCAGTTGCCTGGAGTCCTGATGGTAGACGCATAGCTTGTGGATCGATGGATGGAACTGTTTCTGTTTTTGACGCGGATCGTGGCAAGTTCCTGCACCATCTGGAAGGCCACTTCATGCCCGTGAGGTCTCTTGTGTTTTCTCCAGTTGAGCCACGGTTACTTTTCACGGGCTCAGATGACACCCATGTGCACATGTATGATGCTGAGGGGAAAACATTGGTTGGGGCCATGTCAGGACATGCGAGCTGGGTTCTGAGTGTGGATGTGAGCACAGATGGGGCGGCTCTTGCAACGGGCTCAAGTGATAGAACTGTGAGGCTGTGGGATATCAACATGAGGGGAGCTGTGCAGACAATGAGCAACCACACTGACCAAGTTTGGGGAGTGGCCTTCCGACCAGGAGGAATGCCTGGTAGACTTGCTAGTGTGTCAGATGACAAGAGCATATCGTTATATGACTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000428 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034968 GO:0035327 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0055087 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
WD40

Protein Analysis

318

Amino Acids

34.1

Weight (kDa)

5.62

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 97
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 402
AccI GTMKAC 3 cut(s) 267, 511, 901
AccII CGCG 2 cut(s) 35, 560
AciI CCGC 4 cut(s) 56, 359, 560, 767
AclWI GGATC 3 cut(s) 115, 535, 570
AcoI YGGCCR 2 cut(s) 172, 214
AcsI RAATTY 1 cut(s) 384
AcuI CTGAAG 2 cut(s) 177, 330
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 3 cut(s) 76, 402, 865
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 2 cut(s) 19, 331
AjnI CCWGG 4 cut(s) 156, 494, 883, 895
AleI CACNNNNGTG 1 cut(s) 671
AluBI AGCT 8 cut(s) 7, 148, 203, 246, 353, 521, 732, 830
AluI AGCT 8 cut(s) 7, 148, 203, 246, 353, 521, 732, 830
Alw21I GWGCWC 2 cut(s) 678, 758
Alw26I GTCTC 4 cut(s) 74, 113, 296, 618
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 3 cut(s) 115, 535, 570
AoxI GGCC 5 cut(s) 172, 214, 592, 711, 873
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 5 cut(s) 136, 200, 224, 465, 468
ApoI RAATTY 1 cut(s) 384
Asp700I GAANNNNTTC 1 cut(s) 544
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 67, 711
AsuHPI GGTGA 2 cut(s) 70, 197
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 336
BaeGI GKGCMC 1 cut(s) 678
BalI TGGCCA 1 cut(s) 216
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 2 cut(s) 659, 785
Bbv12I GWGCWC 2 cut(s) 678, 758
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 5 cut(s) 148, 211, 212, 477, 480
BccI CCATC 6 cut(s) 290, 500, 526, 530, 591, 755
BciT130I CCWGG 4 cut(s) 158, 496, 885, 897
BcoDI GTCTC 4 cut(s) 74, 113, 296, 618
BfaI CTAG 4 cut(s) 149, 288, 350, 909
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 6 cut(s) 137, 201, 225, 466, 469, 768
BlsI GCNGC 6 cut(s) 138, 202, 226, 467, 470, 769
Bme1390I CCNGG 4 cut(s) 158, 496, 885, 897
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 67, 711
BmiI GGNNCC 5 cut(s) 68, 69, 343, 445, 712
BmrFI CCNGG 4 cut(s) 158, 496, 885, 897
BmsI GCATC 2 cut(s) 383, 676
BmtI GCTAGC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 65
BpmI CTGGAG 2 cut(s) 517, 612
Bpu10I CCTNAGC 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 769
Bsa29I ATCGAT 1 cut(s) 530
BsaI GGTCTC 3 cut(s) 74, 113, 618
BsaJI CCNNGG 5 cut(s) 157, 181, 228, 453, 638
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 402, 865
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 117, 629
Bse21I CCTNAGG 1 cut(s) 336
BseBI CCWGG 4 cut(s) 158, 496, 885, 897
BseCI ATCGAT 1 cut(s) 530
BseDI CCNNGG 5 cut(s) 157, 181, 228, 453, 638
BseGI GGATG 2 cut(s) 541, 754
BseLI CCNNNNNNNGG 3 cut(s) 76, 402, 865
BseMII CTCAG 4 cut(s) 350, 672, 681, 731
BseNI ACTGG 2 cut(s) 117, 629
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 5 cut(s) 148, 211, 212, 477, 480
BseYI CCCAGC 1 cut(s) 732
BsgI GTGCAG 2 cut(s) 563, 854
Bsh1236I CGCG 2 cut(s) 35, 560
BshFI GGCC 5 cut(s) 174, 216, 594, 713, 875
BshNI GGYRCC 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 530
BsiHKAI GWGCWC 2 cut(s) 678, 758
BsiSI CCGG 2 cut(s) 105, 171
BslFI GGGAC 2 cut(s) 53, 404
BslI CCNNNNNNNGG 3 cut(s) 76, 402, 865
BsmAI GTCTC 4 cut(s) 74, 113, 296, 618
BsmFI GGGAC 2 cut(s) 53, 404
BsmI GAATGC 1 cut(s) 897
BsnI GGCC 5 cut(s) 174, 216, 594, 713, 875
Bso31I GGTCTC 3 cut(s) 74, 113, 618
Bsp1286I GDGCHC 4 cut(s) 659, 678, 758, 785
Bsp143I GATC 3 cut(s) 107, 527, 562
BspACI CCGC 4 cut(s) 56, 359, 560, 767
BspANI GGCC 5 cut(s) 174, 216, 594, 713, 875
BspCNI CTCAG 4 cut(s) 349, 671, 682, 732
BspDI ATCGAT 1 cut(s) 530
BspFNI CGCG 2 cut(s) 35, 560
BspLI GGNNCC 5 cut(s) 68, 69, 343, 445, 712
BspMI ACCTGC 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 3 cut(s) 115, 535, 570
BspT107I GGYRCC 1 cut(s) 341
BspTNI GGTCTC 3 cut(s) 74, 113, 618
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 2 cut(s) 117, 629
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 5 cut(s) 157, 181, 228, 453, 638
BssMI GATC 3 cut(s) 107, 527, 562
Bst2UI CCWGG 4 cut(s) 158, 496, 885, 897
Bst4CI ACNGT 4 cut(s) 124, 544, 642, 799
BstC8I GCNNGC 3 cut(s) 9, 351, 730
BstDEI CTNAG 4 cut(s) 336, 658, 690, 740
BstDSI CCRYGG 2 cut(s) 453, 638
BstF5I GGATG 2 cut(s) 541, 754
BstFNI CGCG 2 cut(s) 35, 560
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 3 cut(s) 110, 530, 565
BstMAI GTCTC 4 cut(s) 74, 113, 296, 618
BstMBI GATC 3 cut(s) 107, 527, 562
BstMWI GCNNNNNNNGC 5 cut(s) 136, 145, 197, 230, 773
BstNI CCWGG 4 cut(s) 158, 496, 885, 897
BstNSI RCATGY 2 cut(s) 682, 728
BstPAI GACNNNNGTC 1 cut(s) 65
BstSCI CCNGG 4 cut(s) 156, 494, 883, 895
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 2 cut(s) 35, 560
BstV1I GCAGC 5 cut(s) 148, 211, 212, 477, 480
Bsu15I ATCGAT 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 5 cut(s) 174, 216, 594, 713, 875
BsuTUI ATCGAT 1 cut(s) 530
BtgI CCRYGG 2 cut(s) 453, 638
BtgZI GCGATG 1 cut(s) 268
BtsCI GGATG 2 cut(s) 541, 754
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 4 cut(s) 110, 175, 201, 852
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 9, 351, 730
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 67, 711
ClaI ATCGAT 1 cut(s) 530
CseI GACGC 3 cut(s) 41, 522, 566
Csp6I GTAC 1 cut(s) 342
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 4 cut(s) 336, 658, 690, 740
DpnI GATC 3 cut(s) 109, 529, 564
DpnII GATC 3 cut(s) 107, 527, 562
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 172, 214
Eco24I GRGCYC 2 cut(s) 659, 785
Eco31I GGTCTC 3 cut(s) 74, 113, 618
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 177, 330
Eco81I CCTNAGG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 4 cut(s) 156, 494, 883, 895
EcoRV GATATC 1 cut(s) 814
EcoT38I GRGCYC 2 cut(s) 659, 785
FaqI GGGAC 2 cut(s) 53, 404
FauI CCCGC 1 cut(s) 352
FblI GTMKAC 3 cut(s) 267, 511, 901
Fnu4HI GCNGC 6 cut(s) 137, 201, 225, 466, 469, 768
FokI GGATG 2 cut(s) 548, 761
FriOI GRGCYC 2 cut(s) 659, 785
Fsp4HI GCNGC 6 cut(s) 137, 201, 225, 466, 469, 768
FspBI CTAG 4 cut(s) 149, 288, 350, 909
GlaI GCGC 1 cut(s) 36
GluI GCNGC 6 cut(s) 137, 201, 225, 466, 469, 768
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 2 cut(s) 517, 612
HaeIII GGCC 5 cut(s) 174, 216, 594, 713, 875
HapII CCGG 2 cut(s) 105, 171
HgaI GACGC 3 cut(s) 41, 522, 566
HhaI GCGC 1 cut(s) 37
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 4 cut(s) 75, 102, 154, 268
HindII GTYRAC 4 cut(s) 75, 102, 154, 268
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 4 cut(s) 44, 269, 406, 499
HpaI GTTAAC 1 cut(s) 102
HpaII CCGG 2 cut(s) 105, 171
HphI GGTGA 2 cut(s) 70, 197
Hpy166II GTNNAC 7 cut(s) 75, 102, 154, 268, 512, 676, 902
Hpy188I TCNGA 5 cut(s) 310, 661, 741, 881, 919
Hpy188III TCNNGA 5 cut(s) 293, 503, 587, 720, 951
Hpy8I GTNNAC 7 cut(s) 75, 102, 154, 268, 512, 676, 902
HpyAV CCTTC 3 cut(s) 434, 584, 886
HpyCH4III ACNGT 4 cut(s) 124, 544, 642, 799
HpyCH4V TGCA 6 cut(s) 11, 374, 580, 676, 776, 835
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 145, 197, 230, 773
HpyF3I CTNAG 4 cut(s) 336, 658, 690, 740
HspAI GCGC 1 cut(s) 35
KflI GGGWCCC 1 cut(s) 67
KpnI GGTACC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 102
Kzo9I GATC 3 cut(s) 107, 527, 562
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 5 cut(s) 148, 211, 212, 477, 480
LweI GCATC 2 cut(s) 383, 676
MaeI CTAG 4 cut(s) 149, 288, 350, 909
MaeIII GTNAC 2 cut(s) 58, 642
MalI GATC 3 cut(s) 109, 529, 564
MboI GATC 3 cut(s) 107, 527, 562
MhlI GDGCHC 4 cut(s) 659, 678, 758, 785
MlsI TGGCCA 1 cut(s) 216
MluCI AATT 3 cut(s) 320, 384, 426
MluNI TGGCCA 1 cut(s) 216
MlyI GAGTC 3 cut(s) 53, 263, 508
MmeI TCCRAC 2 cut(s) 297, 904
Mox20I TGGCCA 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 544
MscI TGGCCA 1 cut(s) 216
MseI TTAA 1 cut(s) 101
MslI CAYNNNNRTG 3 cut(s) 605, 671, 681
Msp20I TGGCCA 1 cut(s) 216
MspI CCGG 2 cut(s) 105, 171
MspR9I CCNGG 4 cut(s) 158, 496, 885, 897
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 4 cut(s) 158, 496, 885, 897
MvnI CGCG 2 cut(s) 35, 560
MwoI GCNNNNNNNGC 5 cut(s) 136, 145, 197, 230, 773
NdeII GATC 3 cut(s) 107, 527, 562
NheI GCTAGC 1 cut(s) 349
NlaIV GGNNCC 5 cut(s) 68, 69, 343, 445, 712
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 2 cut(s) 682, 728
OliI CACNNNNGTG 1 cut(s) 671
PciI ACATGT 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 1 cut(s) 897
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 1 cut(s) 406
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 6 cut(s) 138, 202, 226, 467, 470, 769
PleI GAGTC 3 cut(s) 52, 263, 507
PpsI GAGTC 3 cut(s) 52, 263, 507
PpuMI RGGWCCY 1 cut(s) 67
PscI ACATGT 1 cut(s) 678
PshAI GACNNNNGTC 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 4 cut(s) 156, 494, 883, 895
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 4 cut(s) 156, 494, 883, 895
PspN4I GGNNCC 5 cut(s) 68, 69, 343, 445, 712
PspPI GGNCC 2 cut(s) 67, 711
PspPPI RGGWCCY 1 cut(s) 67
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 3 cut(s) 605, 671, 681
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 6 cut(s) 137, 201, 225, 466, 469, 768
Sau3AI GATC 3 cut(s) 107, 527, 562
Sau96I GGNCC 2 cut(s) 67, 711
SchI GAGTC 3 cut(s) 53, 263, 508
ScrFI CCNGG 4 cut(s) 158, 496, 885, 897
SduI GDGCHC 4 cut(s) 659, 678, 758, 785
SfaNI GCATC 2 cut(s) 383, 676
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 3 cut(s) 605, 671, 681
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
Sse9I AATT 3 cut(s) 320, 384, 426
SsiI CCGC 4 cut(s) 56, 359, 560, 767
SspMI CTAG 4 cut(s) 149, 288, 350, 909
StyD4I CCNGG 4 cut(s) 156, 494, 883, 895
TaaI ACNGT 4 cut(s) 124, 544, 642, 799
TaqI TCGA 6 cut(s) 27, 240, 261, 267, 294, 530
TasI AATT 3 cut(s) 320, 384, 426
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 4 cut(s) 117, 182, 201, 859
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 5 cut(s) 136, 200, 224, 465, 468
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 17, 589
TspGWI ACGGA 2 cut(s) 92, 470
TspRI CASTG 4 cut(s) 117, 182, 201, 859
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 1 cut(s) 384
XceI RCATGY 2 cut(s) 682, 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmiI GTMKAC 3 cut(s) 267, 511, 901
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 4 cut(s) 149, 288, 350, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.