Rroxscaffold_5G00351200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006297
Physical Location & Seq
Forward (+)
27214926 .. 27216438
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351200.1

Sequence Viewer

Length: 690 bp
ATGACCGGACACTGCCTCGGCGTGGCTTCCGTGGCGGCTCACCCCTTGGGCTTCATCGCCGCCTCTGCTTCGCTTGATAGCTTCGTTAGGATCTTCGACGTTGACTCCAACACCACCATCGCCAGTTTCGAGGCGCCGCCCTCCGAAGTCTGGCAATTACGCTTCAATCCCCAGAAGGAACCAAGCCTGCTGACAAAAGCAGCAGCAAGAAGTTTGTGCTATCGGTTGCCTGGAGTCCTGATGGGGAGATGCATAGCTTGTGGCTCCATGGATGGAACTGTTTCTTTTTTCGACACAGATCGCGGCAAGTTCCTGCACCACCTGGAAGGCCACTGCATACCCGTGAGGTCTCTTGTGTTTTCCCCTGTTGGACCGCGGTTACTCTTCACTGGCTCGGACGATCAACATGTGCACATGTATGATGCTGAGGGGAAAACCCAGGTTGGGTCCATGTCAGGGCATAACGGCTGGGTCTTGAGTGTCGATGCAAGCACAGATGGGGCAGCCCTTGCAACTGGTTCAAGTGATAGAACTGTGAAGCTGTGGGATATTGGCATGAAGGCAGCTGTGCAGACAATGAGCAACCATAATGACCAAGTCTGGGGAGTGGCCTTTCGACCTGGAGGAATGCCTGGCAGACTTGCTAGTGTCTCTGATGACAAGAGCATATCACTGTATGACTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000428 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034968 GO:0035327 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0055087 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
WD40

Protein Analysis

229

Amino Acids

24.51

Weight (kDa)

6.1

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 10 - 201 8.3e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 82 - 227 4.8e-29 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 82 - 154 5.9e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 82 - 228 1.6e-29 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 82 - 227 4.5e-20 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 82 - 182 1.8e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 82 - 227 1.5e-17 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 84 - 228 2.6e-12 G protein beta WD-40 repeat protein
WDR55 PF24796 84 - 207 9.5e-14 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 85 - 209 3.3e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 86 - 209 1.6e-08 WDR36/Utp21 first beta-propeller
WD40 PF00400 102 - 141 2.4e-06 WD domain, G-beta repeat
EIF3I PF24805 108 - 207 8.7e-12 EIF3I
Beta-prop_IFT122_1st PF23381 111 - 218 9.2e-07 IFT122 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 115 - 225 2.7e-07 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 125 - 228 7.8e-16 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 127 - 194 6.5e-10 WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 146 - 228 5.8e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 146 - 183 2.5e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 151 - 228 3.5e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 154 - 228 3.2e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AccII CGCG 2 cut(s) 303, 376
AciI CCGC 6 cut(s) 35, 60, 137, 303, 374, 376
AclWI GGATC 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 134
AfiI CCNNNNNNNGG 5 cut(s) 22, 150, 444, 456, 601
AflIII ACRYGT 2 cut(s) 406, 414
AgsI TTSAA 2 cut(s) 166, 522
AjnI CCWGG 5 cut(s) 229, 321, 438, 619, 631
AluBI AGCT 4 cut(s) 81, 257, 541, 566
AluI AGCT 4 cut(s) 81, 257, 541, 566
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 354, 655
Alw44I GTGCAC 1 cut(s) 410
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 328, 609
ApaLI GTGCAC 1 cut(s) 410
ApeKI GCWGC 4 cut(s) 200, 203, 503, 563
Asp700I GAANNNNTTC 1 cut(s) 280
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 2 cut(s) 371, 447
AsuHPI GGTGA 1 cut(s) 32
AvaII GGWCC 2 cut(s) 371, 447
BaeGI GKGCMC 1 cut(s) 414
BanI GGYRCC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 212, 215, 515, 575
BccI CCATC 4 cut(s) 125, 235, 266, 491
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 5 cut(s) 231, 323, 440, 621, 633
BcoDI GTCTC 2 cut(s) 354, 655
BfaI CTAG 1 cut(s) 645
BfoI RGCGCY 1 cut(s) 137
BisI GCNGC 8 cut(s) 36, 60, 137, 201, 204, 304, 504, 564
BlsI GCNGC 8 cut(s) 37, 61, 138, 202, 205, 305, 505, 565
Bme1390I CCNGG 5 cut(s) 231, 323, 440, 621, 633
Bme18I GGWCC 2 cut(s) 371, 447
BmgT120I GGNCC 2 cut(s) 371, 447
BmiI GGNNCC 4 cut(s) 135, 180, 265, 448
BmrFI CCNGG 5 cut(s) 231, 323, 440, 621, 633
BmsI GCATC 3 cut(s) 239, 412, 475
BpmI CTGGAG 2 cut(s) 252, 642
Bpu10I CCTNAGC 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 496
BsaHI GRCGYC 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 6 cut(s) 16, 30, 45, 267, 374, 438
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 150, 444, 456, 601
Bse1I ACTGG 3 cut(s) 123, 394, 520
BseBI CCWGG 5 cut(s) 231, 323, 440, 621, 633
BseDI CCNNGG 6 cut(s) 16, 30, 45, 267, 374, 438
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 5 cut(s) 22, 150, 444, 456, 601
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 3 cut(s) 123, 394, 520
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 4 cut(s) 212, 215, 515, 575
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 299, 590
Bsh1236I CGCG 2 cut(s) 303, 376
BshFI GGCC 2 cut(s) 330, 611
BshNI GGYRCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 5 cut(s) 22, 150, 444, 456, 601
BsmAI GTCTC 2 cut(s) 354, 655
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 2 cut(s) 330, 611
Bso31I GGTCTC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 3 cut(s) 90, 298, 400
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 6 cut(s) 35, 60, 137, 303, 374, 376
BspANI GGCC 2 cut(s) 330, 611
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 2 cut(s) 303, 376
BspLI GGNNCC 4 cut(s) 135, 180, 265, 448
BspPI GGATC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 354
BsrI ACTGG 3 cut(s) 123, 394, 520
BssECI CCNNGG 6 cut(s) 16, 30, 45, 267, 374, 438
BssMI GATC 3 cut(s) 90, 298, 400
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 2 cut(s) 45, 267
Bst2UI CCWGG 5 cut(s) 231, 323, 440, 621, 633
Bst4CI ACNGT 3 cut(s) 280, 535, 675
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 188, 490
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 3 cut(s) 30, 267, 374
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 2 cut(s) 303, 376
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 3 cut(s) 93, 301, 403
BstMAI GTCTC 2 cut(s) 354, 655
BstMBI GATC 3 cut(s) 90, 298, 400
BstMWI GCNNNNNNNGC 3 cut(s) 32, 65, 509
BstNI CCWGG 5 cut(s) 231, 323, 440, 621, 633
BstNSI RCATGY 2 cut(s) 410, 418
BstSCI CCNGG 5 cut(s) 229, 321, 438, 619, 631
BstSLI GKGCMC 1 cut(s) 414
BstUI CGCG 2 cut(s) 303, 376
BstV1I GCAGC 4 cut(s) 212, 215, 515, 575
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 2 cut(s) 330, 611
BtgI CCRYGG 3 cut(s) 30, 267, 374
BtgZI GCGATG 2 cut(s) 40, 103
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 2 cut(s) 10, 331
BtsIMutI CAGTG 4 cut(s) 10, 331, 387, 671
Cac8I GCNNGC 2 cut(s) 188, 490
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 371, 447
Cfr42I CCGCGG 1 cut(s) 377
CviAII CATG 5 cut(s) 268, 407, 415, 451, 556
DdeI CTNAG 1 cut(s) 426
DinI GGCGCC 1 cut(s) 135
DpnI GATC 3 cut(s) 92, 300, 402
DpnII GATC 3 cut(s) 90, 298, 400
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 45, 267
Eco31I GGTCTC 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 371, 447
EcoRII CCWGG 5 cut(s) 229, 321, 438, 619, 631
EcoT14I CCWWGG 2 cut(s) 45, 267
EcoT22I ATGCAT 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 135
EheI GGCGCC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 45, 267
FaeI CATG 5 cut(s) 271, 410, 418, 454, 559
FatI CATG 5 cut(s) 267, 406, 414, 450, 555
Fnu4HI GCNGC 8 cut(s) 36, 60, 137, 201, 204, 304, 504, 564
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 8 cut(s) 36, 60, 137, 201, 204, 304, 504, 564
FspBI CTAG 1 cut(s) 645
GlaI GCGC 1 cut(s) 135
GluI GCNGC 8 cut(s) 36, 60, 137, 201, 204, 304, 504, 564
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 2 cut(s) 252, 642
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 2 cut(s) 330, 611
HapII CCGG 1 cut(s) 6
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 5 cut(s) 271, 410, 418, 454, 559
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 2 cut(s) 104, 234
HpaII CCGG 1 cut(s) 6
HphI GGTGA 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 103, 412
Hpy188I TCNGA 3 cut(s) 145, 397, 655
Hpy188III TCNNGA 3 cut(s) 238, 475, 687
Hpy8I GTNNAC 2 cut(s) 103, 412
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 3 cut(s) 169, 320, 553
HpyCH4III ACNGT 3 cut(s) 280, 535, 675
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 7 cut(s) 252, 316, 336, 412, 488, 512, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 65, 509
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 5 cut(s) 271, 410, 418, 454, 559
HspAI GCGC 1 cut(s) 134
KasI GGCGCC 1 cut(s) 133
KspI CCGCGG 1 cut(s) 377
Kzo9I GATC 3 cut(s) 90, 298, 400
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 4 cut(s) 212, 215, 515, 575
LweI GCATC 3 cut(s) 239, 412, 475
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 378
MalI GATC 3 cut(s) 92, 300, 402
MboI GATC 3 cut(s) 90, 298, 400
MboII GAAGA 2 cut(s) 85, 376
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 1 cut(s) 155
Mly113I GGCGCC 1 cut(s) 134
MlyI GAGTC 2 cut(s) 98, 243
MmeI TCCRAC 2 cut(s) 132, 349
MnlI CCTC 7 cut(s) 26, 73, 124, 151, 339, 421, 617
Mph1103I ATGCAT 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 280
MslI CAYNNNNRTG 2 cut(s) 341, 417
MspA1I CMGCKG 2 cut(s) 376, 566
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 5 cut(s) 231, 323, 440, 621, 633
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 5 cut(s) 231, 323, 440, 621, 633
MvnI CGCG 2 cut(s) 303, 376
MwoI GCNNNNNNNGC 3 cut(s) 32, 65, 509
NarI GGCGCC 1 cut(s) 134
NcoI CCATGG 1 cut(s) 267
NdeII GATC 3 cut(s) 90, 298, 400
NlaIII CATG 5 cut(s) 271, 410, 418, 454, 559
NlaIV GGNNCC 4 cut(s) 135, 180, 265, 448
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 2 cut(s) 410, 418
PciI ACATGT 2 cut(s) 406, 414
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 280
PflFI GACNNNGTC 1 cut(s) 596
PkrI GCNGC 8 cut(s) 37, 61, 138, 202, 205, 305, 505, 565
PleI GAGTC 2 cut(s) 98, 242
PluTI GGCGCC 1 cut(s) 137
PpsI GAGTC 2 cut(s) 98, 242
PscI ACATGT 2 cut(s) 406, 414
Psp6I CCWGG 5 cut(s) 229, 321, 438, 619, 631
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 5 cut(s) 229, 321, 438, 619, 631
PspN4I GGNNCC 4 cut(s) 135, 180, 265, 448
PspPI GGNCC 2 cut(s) 371, 447
PsuI RGATCY 1 cut(s) 90
PsyI GACNNNGTC 1 cut(s) 596
PvuII CAGCTG 1 cut(s) 566
RseI CAYNNNNRTG 2 cut(s) 341, 417
SacII CCGCGG 1 cut(s) 377
SatI GCNGC 8 cut(s) 36, 60, 137, 201, 204, 304, 504, 564
Sau3AI GATC 3 cut(s) 90, 298, 400
Sau96I GGNCC 2 cut(s) 371, 447
SchI GAGTC 2 cut(s) 98, 243
ScrFI CCNGG 5 cut(s) 231, 323, 440, 621, 633
SduI GDGCHC 1 cut(s) 414
SetI ASST 9 cut(s) 83, 102, 259, 324, 350, 444, 543, 568, 622
SfaNI GCATC 3 cut(s) 239, 412, 475
SfoI GGCGCC 1 cut(s) 135
Sfr303I CCGCGG 1 cut(s) 377
SgrBI CCGCGG 1 cut(s) 377
SinI GGWCC 2 cut(s) 371, 447
SmiMI CAYNNNNRTG 2 cut(s) 341, 417
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 1 cut(s) 155
SsiI CCGC 6 cut(s) 35, 60, 137, 303, 374, 376
SspDI GGCGCC 1 cut(s) 133
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 5 cut(s) 229, 321, 438, 619, 631
StyI CCWWGG 2 cut(s) 45, 267
TaaI ACNGT 3 cut(s) 280, 535, 675
TaiI ACGT 1 cut(s) 102
TaqI TCGA 5 cut(s) 96, 129, 291, 483, 616
TasI AATT 1 cut(s) 155
TauI GCSGC 4 cut(s) 38, 62, 139, 306
TscAI CASTG 4 cut(s) 17, 338, 394, 678
TseI GCWGC 4 cut(s) 200, 203, 503, 563
TspDTI ATGAA 2 cut(s) 43, 572
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 4 cut(s) 17, 338, 394, 678
Tth111I GACNNNGTC 1 cut(s) 596
VneI GTGCAC 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 371, 447
XceI RCATGY 2 cut(s) 410, 418
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 1 cut(s) 645
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.