pycom02g06830

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006297
Physical Location & Seq
Forward (+)
4729682 .. 4731418
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06830.1

Sequence Viewer

Length: 954 bp
ATGAAGCTCGCCGGACTGAAATCAATTGACGACGCGCACGACGAGTCGGTATGGGCGGTAACGTGGGTCCCGGCCACCGATTCCCGACCTGCTTTGCTGCTAACCGGGTCGCTGGACGAGACCGTGAGGCTGTGGCAACCGGACGAGCTGGTTAACCGGGGCAAAATGACCGGCCACTGTCTCGGCGTGGCCTCGGTGGCGGCTCACCCCTCCGGCCGCATCGCCGCCTCGGCCTCGCTCGATAGCTTCGTCAGGATCTTTGATGTTGACACCAACAACACCGTGGCCAGTCTCGAAGCCCCGCCGTCTGAAGTGTGGCAATTACGATTCAATCCCCAGGGTACCATGCTAGCTGTGGCAGGTGGGGGAAGTGCATCCGTCAAAGTTTGGGACACTGCCACATGGAATATAGTAGCCACATTGTCGATTCCTCGCCCTGAAGGAACCAAGCCAACTGACAAAAGCAGCAGCAAGAAGTTTGTGCTATCGGTTGCCTGGAGTCCTGATGGGAGACGCATTGCTTGTGGGTCGATGGATGGAACTGTTTCTGTTTTTGATACAGATCGTGCCAAGTTCCTGCACCACCTGGAAGGCCACTTCATGCCAGTGAGGTCTCTCGTGTTTTCTCCCGTTGAGCCCCGGTTGCTCTTCACAGGCTCGGATGATGCCCATGTGCACATGTATGATGCTGAGGGTAAAACCTTGGTTGGGGCTATGTCAGGTCACGCAGGCTGGGTCCTGAGTGTGGATGTGAGCAGTGATGGGGCGGCTGTTGCAACGGGCTCCAGTGATAGAACTGTGAGGCTGTGGGATATCAGCATGAGGGGAGCTGTGCAGACAATGAGCAACCACACTGACCAAGTCTGGGGAGTGGCGTTTCGACCAGGAGGAATGCCTGGTAGACTTGCTAGTGTGTCCGATGACAAGAGTATATCGCTGTACGACTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000428 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034968 GO:0035327 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0055087 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
WD40

Protein Analysis

318

Amino Acids

33.92

Weight (kDa)

5.77

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 7 - 88 2e-06 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 144 3.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 12 - 97 7.8e-06 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 12 - 142 6.2e-20 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 12 - 131 9.7e-16 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 13 - 140 8.8e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 13 - 141 8e-19 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 15 - 138 1.6e-09 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 25 - 132 3.4e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 31 - 218 4.4e-21 WDHD1 first WD40 domain
Beta-prop_EML PF23409 41 - 233 1.1e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR19_1st PF23389 70 - 236 2.5e-09 WDR19 first beta-propeller
Beta-prop_WDR3_2nd PF25172 85 - 315 1.1e-25 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 95 - 236 3.7e-07 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 107 - 271 3.7e-13 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 121 - 289 9e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 122 - 315 1.8e-32 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 152 - 243 1.2e-07 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 153 - 315 6.8e-25 CDC20/Fizzy WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 155 - 233 2.1e-06 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 156 - 209 1.5e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 157 - 316 2.6e-33 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 158 - 270 6.5e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 158 - 295 3.1e-14 WDR55
Beta-prop_RIG_2nd PF23775 158 - 196 9.7e-06 RIG second beta-propeller
Beta-prop_THOC3 PF25174 158 - 241 1.2e-17 THOC3 beta-propeller domain
WD40_Gbeta PF25391 159 - 316 2.2e-14 G protein beta WD-40 repeat protein
WD40 PF00400 159 - 186 1.7e-07 WD domain, G-beta repeat
EIF3I PF24805 196 - 295 3.9e-11 EIF3I
WD40_Prp19 PF24814 212 - 316 1.5e-15 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 215 - 296 6.3e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 216 - 315 1e-09 WDHD1 first WD40 domain
Beta-prop_IFT122_1st PF23381 222 - 306 1e-05 IFT122 first beta-propeller
WD40 PF00400 235 - 271 6e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 237 - 316 3.4e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 238 - 316 3.6e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 242 - 316 4.9e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 350
Acc36I ACCTGC 2 cut(s) 97, 350
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AccI GTMKAC 1 cut(s) 901
AccII CGCG 1 cut(s) 35
AciI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 4 cut(s) 72, 172, 214, 285
AcuI CTGAAG 2 cut(s) 330, 459
AfaI GTAC 2 cut(s) 343, 941
AfiI CCNNNNNNNGG 3 cut(s) 708, 745, 865
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 1 cut(s) 331
AjnI CCWGG 5 cut(s) 336, 494, 585, 883, 895
AluBI AGCT 5 cut(s) 7, 148, 246, 353, 830
AluI AGCT 5 cut(s) 7, 148, 246, 353, 830
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 5 cut(s) 113, 185, 296, 505, 618
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 1 cut(s) 263
AoxI GGCC 7 cut(s) 72, 172, 189, 214, 231, 285, 592
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 97, 465, 468
Asp700I GAANNNNTTC 1 cut(s) 544
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 67, 736
AsuC2I CCSGG 4 cut(s) 71, 106, 158, 640
AsuHPI GGTGA 1 cut(s) 197
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 2 cut(s) 67, 736
BaeGI GKGCMC 1 cut(s) 678
BalI TGGCCA 1 cut(s) 287
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 2 cut(s) 639, 785
BauI CACGAG 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 678
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 84, 477, 480
BccI CCATC 4 cut(s) 500, 526, 530, 755
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 5 cut(s) 338, 496, 587, 885, 897
BcnI CCSGG 4 cut(s) 71, 106, 158, 640
BcoDI GTCTC 5 cut(s) 113, 185, 296, 505, 618
BfaI CTAG 2 cut(s) 350, 909
BfuAI ACCTGC 2 cut(s) 97, 350
BglI GCCNNNNNGGC 2 cut(s) 197, 230
BisI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
BlsI GCNGC 7 cut(s) 99, 202, 218, 226, 467, 470, 769
Bme1390I CCNGG 9 cut(s) 71, 106, 158, 338, 496, 587, 640, 885, 897
Bme18I GGWCC 2 cut(s) 67, 736
BmgT120I GGNCC 2 cut(s) 67, 736
BmiI GGNNCC 6 cut(s) 68, 69, 343, 445, 737, 784
BmrFI CCNGG 9 cut(s) 71, 106, 158, 338, 496, 587, 640, 885, 897
BmsI GCATC 4 cut(s) 228, 383, 655, 676
BmtI GCTAGC 1 cut(s) 353
BpmI CTGGAG 2 cut(s) 517, 769
Bpu10I CCTNAGC 1 cut(s) 690
BpuMI CCSGG 4 cut(s) 71, 106, 158, 640
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 113, 618
BsaJI CCNNGG 8 cut(s) 157, 192, 228, 282, 336, 337, 638, 702
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 708, 745, 865
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 3 cut(s) 288, 605, 786
Bse3DI GCAATG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 5 cut(s) 338, 496, 587, 885, 897
BseDI CCNNGG 8 cut(s) 157, 192, 228, 282, 336, 337, 638, 702
BseGI GGATG 4 cut(s) 374, 541, 667, 754
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 3 cut(s) 708, 745, 865
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 681, 731
BseNI ACTGG 3 cut(s) 288, 605, 786
BseSI GKGCMC 1 cut(s) 678
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 3 cut(s) 84, 477, 480
BseYI CCCAGC 1 cut(s) 732
BsgI GTGCAG 2 cut(s) 563, 854
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 7 cut(s) 74, 174, 191, 216, 233, 287, 594
BshNI GGYRCC 1 cut(s) 341
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 8 cut(s) 12, 71, 105, 140, 157, 171, 213, 640
BslFI GGGAC 2 cut(s) 53, 404
BslI CCNNNNNNNGG 3 cut(s) 708, 745, 865
BsmAI GTCTC 5 cut(s) 113, 185, 296, 505, 618
BsmBI CGTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 53, 404
BsmI GAATGC 1 cut(s) 897
BsnI GGCC 7 cut(s) 74, 174, 191, 216, 233, 287, 594
Bso31I GGTCTC 2 cut(s) 113, 618
Bsp1286I GDGCHC 3 cut(s) 639, 678, 785
Bsp143I GATC 2 cut(s) 255, 562
BspACI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
BspANI GGCC 7 cut(s) 74, 174, 191, 216, 233, 287, 594
BspCNI CTCAG 2 cut(s) 682, 732
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 6 cut(s) 68, 69, 343, 445, 737, 784
BspMI ACCTGC 2 cut(s) 97, 350
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 1 cut(s) 263
BspQI GCTCTTC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 341
BspTNI GGTCTC 2 cut(s) 113, 618
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 3 cut(s) 288, 605, 786
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 8 cut(s) 157, 192, 228, 282, 336, 337, 638, 702
BssMI GATC 2 cut(s) 255, 562
BssSI CACGAG 1 cut(s) 617
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 5 cut(s) 338, 496, 587, 885, 897
Bst4CI ACNGT 5 cut(s) 124, 179, 283, 544, 799
Bst6I CTCTTC 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 9, 351, 730
BstDEI CTNAG 2 cut(s) 690, 740
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 4 cut(s) 374, 541, 667, 754
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 2 cut(s) 258, 565
BstMAI GTCTC 5 cut(s) 113, 185, 296, 505, 618
BstMBI GATC 2 cut(s) 255, 562
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 197, 230, 643, 773
BstNI CCWGG 5 cut(s) 338, 496, 587, 885, 897
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 9 cut(s) 69, 104, 156, 336, 494, 585, 638, 883, 895
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 84, 477, 480
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 7 cut(s) 74, 174, 191, 216, 233, 287, 594
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 4 cut(s) 374, 541, 667, 754
BtsI GCAGTG 2 cut(s) 393, 763
BtsIMutI CAGTG 6 cut(s) 175, 393, 612, 763, 793, 852
BveI ACCTGC 2 cut(s) 97, 350
Cac8I GCNNGC 3 cut(s) 9, 351, 730
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 67, 736
CseI GACGC 2 cut(s) 41, 522
Csp6I GTAC 2 cut(s) 342, 940
CviAII CATG 6 cut(s) 346, 402, 601, 671, 679, 820
CviQI GTAC 2 cut(s) 342, 940
DdeI CTNAG 2 cut(s) 690, 740
DpnI GATC 2 cut(s) 257, 564
DpnII GATC 2 cut(s) 255, 562
EaeI YGGCCR 4 cut(s) 72, 172, 214, 285
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 702
Eco24I GRGCYC 2 cut(s) 639, 785
Eco31I GGTCTC 2 cut(s) 113, 618
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 2 cut(s) 67, 736
Eco52I CGGCCG 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 330, 459
EcoO109I RGGNCCY 2 cut(s) 67, 736
EcoRII CCWGG 5 cut(s) 336, 494, 585, 883, 895
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 702
EcoT38I GRGCYC 2 cut(s) 639, 785
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 505
FaeI CATG 6 cut(s) 349, 405, 604, 674, 682, 823
FaqI GGGAC 2 cut(s) 53, 404
FatI CATG 6 cut(s) 345, 401, 600, 670, 678, 819
FauI CCCGC 1 cut(s) 309
FblI GTMKAC 1 cut(s) 901
Fnu4HI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
FokI GGATG 4 cut(s) 361, 548, 674, 761
FriOI GRGCYC 2 cut(s) 639, 785
Fsp4HI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
FspBI CTAG 2 cut(s) 350, 909
GlaI GCGC 1 cut(s) 36
GluI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 2 cut(s) 517, 769
HaeIII GGCC 7 cut(s) 74, 174, 191, 216, 233, 287, 594
HapII CCGG 8 cut(s) 12, 71, 105, 140, 157, 171, 213, 640
HgaI GACGC 2 cut(s) 41, 522
HhaI GCGC 1 cut(s) 37
Hin1II CATG 6 cut(s) 349, 405, 604, 674, 682, 823
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 2 cut(s) 154, 268
HindII GTYRAC 2 cut(s) 154, 268
HinfI GANTC 5 cut(s) 44, 80, 327, 427, 499
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 8 cut(s) 12, 71, 105, 140, 157, 171, 213, 640
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 4 cut(s) 154, 268, 676, 902
Hpy188I TCNGA 3 cut(s) 310, 661, 919
Hpy188III TCNNGA 6 cut(s) 84, 253, 293, 503, 739, 951
Hpy8I GTNNAC 4 cut(s) 154, 268, 676, 902
Hpy99I CGWCG 2 cut(s) 35, 44
HpyAV CCTTC 2 cut(s) 434, 584
HpyCH4III ACNGT 5 cut(s) 124, 179, 283, 544, 799
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 374, 580, 676, 776, 835
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 230, 643, 773
HpyF3I CTNAG 2 cut(s) 690, 740
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 6 cut(s) 349, 405, 604, 674, 682, 823
HspAI GCGC 1 cut(s) 35
KflI GGGWCCC 1 cut(s) 67
KpnI GGTACC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 255, 562
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 2 cut(s) 788, 827
Lsp1109I GCAGC 3 cut(s) 84, 477, 480
LweI GCATC 4 cut(s) 228, 383, 655, 676
MaeI CTAG 2 cut(s) 350, 909
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 58, 722
MalI GATC 2 cut(s) 257, 564
MboI GATC 2 cut(s) 255, 562
MboII GAAGA 1 cut(s) 640
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 3 cut(s) 639, 678, 785
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 2 cut(s) 24, 320
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 2 cut(s) 53, 508
Mox20I TGGCCA 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 544
MscI TGGCCA 1 cut(s) 287
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 2 cut(s) 605, 681
Msp20I TGGCCA 1 cut(s) 287
MspI CCGG 8 cut(s) 12, 71, 105, 140, 157, 171, 213, 640
MspR9I CCNGG 9 cut(s) 71, 106, 158, 338, 496, 587, 640, 885, 897
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 5 cut(s) 338, 496, 587, 885, 897
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 4 cut(s) 197, 230, 643, 773
NciI CCSGG 4 cut(s) 71, 106, 158, 640
NdeII GATC 2 cut(s) 255, 562
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 6 cut(s) 349, 405, 604, 674, 682, 823
NlaIV GGNNCC 6 cut(s) 68, 69, 343, 445, 737, 784
NmeAIII GCCGAG 2 cut(s) 162, 209
NmuCI GTSAC 1 cut(s) 722
NspI RCATGY 1 cut(s) 682
PaqCI CACCTGC 1 cut(s) 350
PasI CCCWGGG 1 cut(s) 337
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 653
PcsI WCGNNNNNNNCGW 3 cut(s) 36, 39, 246
PctI GAATGC 1 cut(s) 897
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 3 cut(s) 80, 327, 427
PflFI GACNNNGTC 1 cut(s) 860
PkrI GCNGC 7 cut(s) 99, 202, 218, 226, 467, 470, 769
PleI GAGTC 2 cut(s) 52, 507
PpsI GAGTC 2 cut(s) 52, 507
PpuMI RGGWCCY 2 cut(s) 67, 736
PscI ACATGT 1 cut(s) 678
Psp5II RGGWCCY 2 cut(s) 67, 736
Psp6I CCWGG 5 cut(s) 336, 494, 585, 883, 895
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 5 cut(s) 336, 494, 585, 883, 895
PspN4I GGNNCC 6 cut(s) 68, 69, 343, 445, 737, 784
PspPI GGNCC 2 cut(s) 67, 736
PspPPI RGGWCCY 2 cut(s) 67, 736
PsuI RGATCY 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 860
RsaI GTAC 2 cut(s) 343, 941
RsaNI GTAC 2 cut(s) 342, 940
RseI CAYNNNNRTG 2 cut(s) 605, 681
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
Sau3AI GATC 2 cut(s) 255, 562
Sau96I GGNCC 2 cut(s) 67, 736
SchI GAGTC 2 cut(s) 53, 508
ScrFI CCNGG 9 cut(s) 71, 106, 158, 338, 496, 587, 640, 885, 897
SduI GDGCHC 3 cut(s) 639, 678, 785
SfaNI GCATC 4 cut(s) 228, 383, 655, 676
SinI GGWCC 2 cut(s) 67, 736
SmiMI CAYNNNNRTG 2 cut(s) 605, 681
Sse9I AATT 2 cut(s) 24, 320
SsiI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
SspMI CTAG 2 cut(s) 350, 909
StyD4I CCNGG 9 cut(s) 69, 104, 156, 336, 494, 585, 638, 883, 895
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 5 cut(s) 124, 179, 283, 544, 799
TaiI ACGT 1 cut(s) 65
TaqI TCGA 5 cut(s) 240, 294, 425, 530, 880
TasI AATT 2 cut(s) 24, 320
TauI GCSGC 4 cut(s) 203, 219, 227, 770
TfiI GAWTC 3 cut(s) 80, 327, 427
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 6 cut(s) 182, 400, 612, 763, 793, 859
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 3 cut(s) 97, 465, 468
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 2 cut(s) 17, 589
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 6 cut(s) 182, 400, 612, 763, 793, 859
Tth111I GACNNNGTC 1 cut(s) 860
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 67, 736
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 280, 352
XmiI GTMKAC 1 cut(s) 901
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 2 cut(s) 350, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.