pycom15g18890

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006297
Physical Location & Seq
Forward (+)
13399039 .. 13400699
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g18890.2

Sequence Viewer

Length: 954 bp
ATGAAGCTAGCCGGACTGAAATCAGTCGAGGACGCGCACGATGAGTCGGTATGGGCGGTGACGTGGGTGCCGGCCACCGAGTCCCGACCCTCATTGCTGCTAACCGGGTCACTGGACGAGACCGTGAGGCTGTGGCAACCGGACGAGCTGGTGAACCGGGGAAAAATGACCGGCCACTGCCTCGGCGTGGCTTCGGTGGCGGCCCACCCCTCCGGCCGCATCGCCGCCTCGGCTTCGCTGGATAGCTTCGTCAGAGTATTTGATGTTGATACTAACAATACCGTGGCCAGTCTCGAAGCCCCGCCGTCTGAAGTGTGGCAATTAAGATTCAATCCCCAGGGTACCATGCTAGCTGTGGCAGGTGGGGGAAGTGCATCAGTCAAAGTTTGGGACACTGCCACATGGCATATAGTAGCCACATTGTCAATTCCTCGCCCCGAAGGAACCAAGCCAACTGATAAAAGCAGCAGCAAGAAGTTTGTGCTATCGGTTGCCTGGAGTCCTGATGGGAGACGCATTGCTTGTGGCTCTATGGATGGAACTGTTTCTGTTTTCGATGCAGATCGTGCCAAGTTCCTGCACCACCTGGAAGGCCACTTCATGCCAGTGAGGTCTCTCGTGTTTTCACCAGTTGAGCCAAGGTTACTCTTCACAGGCTCAGATGATACCCATGTGCACATGTATGACGCTGAAGGAAAAACCTTGGTTGGAGCTATGTCAGGTCACGCAGGATGGGTCCTGAGTGTGGATGTGAGTAGTGACGGGGCGGCTGTTGCAACGGGCTCAAGTGATAGAACCGTGAGGCTGTGGGATATCAGCATGAGGGGAGCTGTGCAGACAATGAGCAACCACACTGACCAAGTCTGGGGAGTGGCGTTTCGACCAGGAGGAATGCCTGGTAGACTTGCAAGTGTGTCAGATGACAAGAGCATATCGCTGTACGACTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000428 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009909 GO:0009910 GO:0009987 GO:0010452 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0016043 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034968 GO:0035327 GO:0036211 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0055087 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0080090 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
WD40

Protein Analysis

318

Amino Acids

33.96

Weight (kDa)

5.86

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 350
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 67, 341
AccI GTMKAC 1 cut(s) 901
AccII CGCG 1 cut(s) 35
AciI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
AcoI YGGCCR 4 cut(s) 72, 172, 214, 285
AcuI CTGAAG 2 cut(s) 330, 711
AfaI GTAC 2 cut(s) 343, 941
AfiI CCNNNNNNNGG 3 cut(s) 187, 745, 865
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 1 cut(s) 331
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 5 cut(s) 336, 494, 585, 883, 895
AluBI AGCT 6 cut(s) 7, 148, 246, 353, 713, 830
AluI AGCT 6 cut(s) 7, 148, 246, 353, 713, 830
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 4 cut(s) 113, 296, 505, 618
Alw44I GTGCAC 1 cut(s) 674
AoxI GGCC 6 cut(s) 72, 172, 201, 214, 285, 592
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 97, 465, 468
Asp700I GAANNNNTTC 1 cut(s) 544
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 202, 736
AsuC2I CCSGG 2 cut(s) 106, 158
AsuHPI GGTGA 3 cut(s) 70, 163, 618
AsuNHI GCTAGC 2 cut(s) 7, 349
AvaII GGWCC 1 cut(s) 736
BaeGI GKGCMC 1 cut(s) 678
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BalI TGGCCA 1 cut(s) 287
BanI GGYRCC 2 cut(s) 67, 341
BanII GRGCYC 1 cut(s) 785
BauI CACGAG 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 3 cut(s) 84, 477, 480
BccI CCATC 3 cut(s) 500, 530, 726
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 5 cut(s) 338, 496, 587, 885, 897
BcnI CCSGG 2 cut(s) 106, 158
BcoDI GTCTC 4 cut(s) 113, 296, 505, 618
BfaI CTAG 2 cut(s) 8, 350
BfuAI ACCTGC 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
BlsI GCNGC 7 cut(s) 99, 202, 218, 226, 467, 470, 769
Bme1390I CCNGG 7 cut(s) 106, 158, 338, 496, 587, 885, 897
Bme18I GGWCC 1 cut(s) 736
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 202, 736
BmiI GGNNCC 4 cut(s) 69, 343, 445, 737
BmrFI CCNGG 7 cut(s) 106, 158, 338, 496, 587, 885, 897
BmsI GCATC 3 cut(s) 228, 383, 547
BmtI GCTAGC 2 cut(s) 11, 353
BpmI CTGGAG 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 769
BpuMI CCSGG 2 cut(s) 106, 158
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 113, 618
BsaJI CCNNGG 8 cut(s) 157, 181, 228, 282, 336, 337, 638, 702
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 745, 865
Bse118I RCCGGY 2 cut(s) 70, 170
Bse1I ACTGG 4 cut(s) 117, 288, 605, 629
Bse3DI GCAATG 2 cut(s) 92, 516
Bse8I GATNNNNATC 1 cut(s) 561
BseBI CCWGG 5 cut(s) 338, 496, 587, 885, 897
BseDI CCNNGG 8 cut(s) 157, 181, 228, 282, 336, 337, 638, 702
BseGI GGATG 3 cut(s) 541, 737, 754
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 3 cut(s) 187, 745, 865
BseMI GCAATG 2 cut(s) 92, 516
BseMII CTCAG 2 cut(s) 672, 731
BseNI ACTGG 4 cut(s) 117, 288, 605, 629
BseSI GKGCMC 1 cut(s) 678
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 3 cut(s) 84, 477, 480
BsgI GTGCAG 2 cut(s) 563, 854
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 6 cut(s) 74, 174, 203, 216, 287, 594
BshNI GGYRCC 2 cut(s) 67, 341
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 7 cut(s) 12, 71, 105, 140, 157, 171, 213
BslFI GGGAC 2 cut(s) 67, 404
BslI CCNNNNNNNGG 3 cut(s) 187, 745, 865
BsmAI GTCTC 4 cut(s) 113, 296, 505, 618
BsmBI CGTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 67, 404
BsmI GAATGC 1 cut(s) 897
BsnI GGCC 6 cut(s) 74, 174, 203, 216, 287, 594
Bso31I GGTCTC 2 cut(s) 113, 618
Bsp1286I GDGCHC 2 cut(s) 678, 785
Bsp143I GATC 1 cut(s) 562
BspACI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
BspANI GGCC 6 cut(s) 74, 174, 203, 216, 287, 594
BspCNI CTCAG 2 cut(s) 671, 732
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 4 cut(s) 69, 343, 445, 737
BspMI ACCTGC 1 cut(s) 350
BspOI GCTAGC 2 cut(s) 11, 353
BspT107I GGYRCC 2 cut(s) 67, 341
BspTNI GGTCTC 2 cut(s) 113, 618
BsrDI GCAATG 2 cut(s) 92, 516
BsrFI RCCGGY 2 cut(s) 70, 170
BsrI ACTGG 4 cut(s) 117, 288, 605, 629
BssAI RCCGGY 2 cut(s) 70, 170
BssECI CCNNGG 8 cut(s) 157, 181, 228, 282, 336, 337, 638, 702
BssMI GATC 1 cut(s) 562
BssSI CACGAG 1 cut(s) 617
BssT1I CCWWGG 2 cut(s) 638, 702
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 5 cut(s) 338, 496, 587, 885, 897
Bst4CI ACNGT 4 cut(s) 124, 283, 544, 799
Bst6I CTCTTC 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 9, 72, 351
BstDEI CTNAG 2 cut(s) 658, 740
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 3 cut(s) 541, 737, 754
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 1 cut(s) 565
BstMAI GTCTC 4 cut(s) 113, 296, 505, 618
BstMBI GATC 1 cut(s) 562
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 197, 230, 566, 773
BstNI CCWGG 5 cut(s) 338, 496, 587, 885, 897
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 7 cut(s) 104, 156, 336, 494, 585, 883, 895
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 84, 477, 480
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 6 cut(s) 74, 174, 203, 216, 287, 594
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 3 cut(s) 541, 737, 754
BtsI GCAGTG 2 cut(s) 175, 393
BtsIMutI CAGTG 5 cut(s) 110, 175, 393, 612, 852
BveI ACCTGC 1 cut(s) 350
Cac8I GCNNGC 3 cut(s) 9, 72, 351
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 2 cut(s) 70, 170
Cfr13I GGNCC 2 cut(s) 202, 736
CseI GACGC 3 cut(s) 41, 522, 695
Csp6I GTAC 2 cut(s) 342, 940
CviAII CATG 6 cut(s) 346, 402, 601, 671, 679, 820
CviQI GTAC 2 cut(s) 342, 940
DdeI CTNAG 2 cut(s) 658, 740
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
EaeI YGGCCR 4 cut(s) 72, 172, 214, 285
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 638, 702
Eco24I GRGCYC 1 cut(s) 785
Eco31I GGTCTC 2 cut(s) 113, 618
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 736
Eco52I CGGCCG 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 330, 711
EcoO109I RGGNCCY 1 cut(s) 736
EcoRII CCWGG 5 cut(s) 336, 494, 585, 883, 895
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 638, 702
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 2 cut(s) 638, 702
Esp3I CGTCTC 1 cut(s) 505
FaeI CATG 6 cut(s) 349, 405, 604, 674, 682, 823
FaqI GGGAC 2 cut(s) 67, 404
FatI CATG 6 cut(s) 345, 401, 600, 670, 678, 819
FauI CCCGC 1 cut(s) 309
FblI GTMKAC 1 cut(s) 901
Fnu4HI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
FokI GGATG 3 cut(s) 548, 744, 761
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
FspBI CTAG 2 cut(s) 8, 350
GlaI GCGC 1 cut(s) 36
GluI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
GsuI CTGGAG 1 cut(s) 517
HaeIII GGCC 6 cut(s) 74, 174, 203, 216, 287, 594
HapII CCGG 7 cut(s) 12, 71, 105, 140, 157, 171, 213
HgaI GACGC 3 cut(s) 41, 522, 695
HhaI GCGC 1 cut(s) 37
Hin1II CATG 6 cut(s) 349, 405, 604, 674, 682, 823
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HinfI GANTC 4 cut(s) 44, 80, 327, 499
HpaII CCGG 7 cut(s) 12, 71, 105, 140, 157, 171, 213
HphI GGTGA 3 cut(s) 70, 163, 618
Hpy166II GTNNAC 3 cut(s) 154, 676, 902
Hpy188I TCNGA 4 cut(s) 254, 310, 661, 919
Hpy188III TCNNGA 5 cut(s) 84, 293, 503, 739, 951
Hpy8I GTNNAC 3 cut(s) 154, 676, 902
HpyAV CCTTC 3 cut(s) 434, 584, 686
HpyCH4III ACNGT 4 cut(s) 124, 283, 544, 799
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 7 cut(s) 374, 560, 580, 676, 776, 835, 908
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 230, 566, 773
HpyF3I CTNAG 2 cut(s) 658, 740
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 6 cut(s) 349, 405, 604, 674, 682, 823
HspAI GCGC 1 cut(s) 35
KpnI GGTACC 1 cut(s) 345
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 1 cut(s) 562
LmnI GCTCC 2 cut(s) 710, 827
Lsp1109I GCAGC 3 cut(s) 84, 477, 480
LweI GCATC 3 cut(s) 228, 383, 547
MaeI CTAG 2 cut(s) 8, 350
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 5 cut(s) 58, 108, 642, 722, 758
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 678, 785
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 2 cut(s) 320, 426
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 3 cut(s) 53, 89, 508
MmeI TCCRAC 1 cut(s) 688
Mox20I TGGCCA 1 cut(s) 287
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 544
MscI TGGCCA 1 cut(s) 287
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 605, 681
Msp20I TGGCCA 1 cut(s) 287
MspI CCGG 7 cut(s) 12, 71, 105, 140, 157, 171, 213
MspR9I CCNGG 7 cut(s) 106, 158, 338, 496, 587, 885, 897
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 5 cut(s) 338, 496, 587, 885, 897
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 4 cut(s) 197, 230, 566, 773
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 2 cut(s) 106, 158
NdeII GATC 1 cut(s) 562
NgoMIV GCCGGC 1 cut(s) 70
NheI GCTAGC 2 cut(s) 7, 349
NlaIII CATG 6 cut(s) 349, 405, 604, 674, 682, 823
NlaIV GGNNCC 4 cut(s) 69, 343, 445, 737
NmeAIII GCCGAG 2 cut(s) 162, 209
NmuCI GTSAC 4 cut(s) 58, 108, 722, 758
NspI RCATGY 1 cut(s) 682
PaqCI CACCTGC 1 cut(s) 350
PasI CCCWGGG 1 cut(s) 337
PciI ACATGT 1 cut(s) 678
PctI GAATGC 1 cut(s) 897
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 544
PfeI GAWTC 1 cut(s) 327
PflFI GACNNNGTC 1 cut(s) 860
PkrI GCNGC 7 cut(s) 99, 202, 218, 226, 467, 470, 769
PleI GAGTC 3 cut(s) 52, 88, 507
PpsI GAGTC 3 cut(s) 52, 88, 507
PpuMI RGGWCCY 1 cut(s) 736
PscI ACATGT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 736
Psp6I CCWGG 5 cut(s) 336, 494, 585, 883, 895
PspGI CCWGG 5 cut(s) 336, 494, 585, 883, 895
PspN4I GGNNCC 4 cut(s) 69, 343, 445, 737
PspPI GGNCC 2 cut(s) 202, 736
PspPPI RGGWCCY 1 cut(s) 736
PsyI GACNNNGTC 1 cut(s) 860
RsaI GTAC 2 cut(s) 343, 941
RsaNI GTAC 2 cut(s) 342, 940
RseI CAYNNNNRTG 2 cut(s) 605, 681
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 7 cut(s) 98, 201, 217, 225, 466, 469, 768
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 2 cut(s) 202, 736
SchI GAGTC 3 cut(s) 53, 89, 508
ScrFI CCNGG 7 cut(s) 106, 158, 338, 496, 587, 885, 897
SduI GDGCHC 2 cut(s) 678, 785
SfaNI GCATC 3 cut(s) 228, 383, 547
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 2 cut(s) 605, 681
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
Sse9I AATT 2 cut(s) 320, 426
SsiI CCGC 6 cut(s) 56, 200, 217, 225, 302, 767
SspMI CTAG 2 cut(s) 8, 350
StyD4I CCNGG 7 cut(s) 104, 156, 336, 494, 585, 883, 895
StyI CCWWGG 2 cut(s) 638, 702
TaaI ACNGT 4 cut(s) 124, 283, 544, 799
TaiI ACGT 1 cut(s) 65
TaqI TCGA 4 cut(s) 27, 294, 555, 880
TasI AATT 2 cut(s) 320, 426
TauI GCSGC 4 cut(s) 203, 219, 227, 770
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 5 cut(s) 117, 182, 400, 612, 859
TseFI GTSAC 4 cut(s) 58, 108, 722, 758
TseI GCWGC 3 cut(s) 97, 465, 468
Tsp45I GTSAC 4 cut(s) 58, 108, 722, 758
TspDTI ATGAA 2 cut(s) 17, 589
TspRI CASTG 5 cut(s) 117, 182, 400, 612, 859
Tth111I GACNNNGTC 1 cut(s) 860
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 736
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 901
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 2 cut(s) 8, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.