FvH4_2g00590

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006215
Physical Location & Seq
Reverse (-)
609578 .. 614700
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00590.t2

Sequence Viewer

Length: 1461 bp
ATGTCTTTGTTCTTCCTCATCTCTGCAGTGTTATCATTGAGCACTAGTATTGGTAATGTTCACTTCGTACAAGCAGCCAACCCAAACTACAGAGATGCCCTTGCAAAGTCCATACTCTTCTTCCAGGGACAGAGGTCAGGCAAGCTCTCCTCCCAGCAAATTACCTGGAGGTCCAATTCTGCACTTTCCGATGGTCGTCCCGCAAACGTGGACTTAACCGGAGGATACTATGATGGTGGAGACAACGTCAAATTCAACTTCCCAATGGCATTCACCACCACAATGCTATCATGGGGATCACTTGAATATAGCAAGAAAATGGGTTCCCAACTAACGAATGCCCGAACCGCAATCCGATGGTCCGCAAACTACCTTCTAAAGTGTGCTCGAGCCACGCCCGATAGGCTCTACGTTGGGGTCGGGGACCCTAACGTAGACCACAAGTGTTGGGAAAGGCCTGAAGACATGGACACGGCTCGAACCGTATACTGGGTGTCACCTAGTAATCCCGGTTCGGATGTTGCTGGAGAGACGGCGGCCGCATTGGCTGCTGCTTCTATGGTTTTCCGAAAGGTTGACCCCAAGTATTCAAAACTGCTAATAAGGACAGCCAAGAAAGTATTTCAGTTTGCAATTCAGTATAGAGGTGCCTATAGTGATTCACTCGGTTCTGCAGTTTGTCCATTTTACTGCTCATATTCTGGATACAAGGATGAGCTAGTGTGGGGAGCTGCATGGCTTCTTAGAGCAACAAATGACGTCTTTTACTTCAATTTCTTGAAATCCATGGGTGGTGATTCACCGGATATATTCAGCTGGGACAACAAATTTGCTGGTGCTTATGCTCTGCTTTCAAGGAGGGCAGTGTTGAACAATGACAAGAAATTTGACTCGTATAAACAAAAAGCTGAGCAATTTATGTGTCGGATTTTGCCCAACTCACCCTCTTCAAGCACACAATATACAAAAGGAGGGCTCATGTTCAAGCTGCCTCAAAGTAACCTACAATATGTCGCCTCAATCACATTCTTGCTCACTACCTATTCCAAGTACATGTCTGCCACACAGCACACGTTTAACTGCGGCAACCTTGTTGTCACTTCAAGCACCTTAAGAAACCTTGCCAAGAGACAGGTGGACTACATATTAGGGGTAAACCCACTGAAGATGTCCTACATGGTAGGGTATGGACCAAGCTATCCCAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCATTGGCAAGTCATCCAACAACCATAAGTTGTAAAGCTGGTTTCCAATCATTCTATTATTCATCAAATCCAAATCCTAACATCTTAGTTGGAGCTGTTGTTGGAGGTCCAAATCAGAATGATGAGTTTCAAGATGATCGCGATGATTATAGTCATTCTGAGCCTGCAACATACATAAATGGTGCGATAGTCGGACCAATAGCATATTTTGCAAATGTCAAATGA

Protein Analysis

487

Amino Acids

53.84

Weight (kDa)

9.3

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 477 5.8e-155 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 762
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 2 cut(s) 435, 486
AccII CGCG 1 cut(s) 1377
AciI CCGC 6 cut(s) 201, 348, 363, 536, 540, 1083
AclWI GGATC 2 cut(s) 304, 1231
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 4 cut(s) 251, 827, 884, 1209
AcuI CTGAAG 2 cut(s) 480, 1184
AcyI GRCGYC 1 cut(s) 759
AfaI GTAC 2 cut(s) 69, 1052
AfiI CCNNNNNNNGG 1 cut(s) 489
AflII CTTAAG 1 cut(s) 1111
AflIII ACRYGT 2 cut(s) 1053, 1071
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 2 cut(s) 123, 164
AluBI AGCT 9 cut(s) 145, 718, 731, 816, 908, 988, 1197, 1274, 1331
AluI AGCT 9 cut(s) 145, 718, 731, 816, 908, 988, 1197, 1274, 1331
Alw21I GWGCWC 2 cut(s) 44, 388
Alw26I GTCTC 3 cut(s) 234, 524, 1123
AlwI GGATC 2 cut(s) 304, 1231
Ama87I CYCGRG 1 cut(s) 387
AoxI GGCC 2 cut(s) 455, 537
ApeKI GCWGC 5 cut(s) 74, 548, 551, 731, 988
ApoI RAATTY 4 cut(s) 251, 827, 884, 1209
ArsI GACNNNNNNTTYG 4 cut(s) 812, 844, 869, 901
AspS9I GGNCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 6 cut(s) 265, 489, 792, 806, 933, 1205
AvaI CYCGRG 1 cut(s) 387
AvaII GGWCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 978
BarI GAAGNNNNNNTAC 2 cut(s) 1157, 1189
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 44, 388
BbvI GCAGC 5 cut(s) 86, 535, 538, 718, 975
BccI CCATC 3 cut(s) 185, 227, 351
BceAI ACGGC 2 cut(s) 489, 549
BciT130I CCWGG 2 cut(s) 125, 166
BciVI GTATCC 2 cut(s) 218, 698
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 3 cut(s) 234, 524, 1123
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 3 cut(s) 45, 501, 719
BfmI CTRYAG 4 cut(s) 24, 88, 652, 672
BfrI CTTAAG 1 cut(s) 1111
BfuI GTATCC 2 cut(s) 218, 698
BglI GCCNNNNNGGC 2 cut(s) 403, 545
BisI GCNGC 8 cut(s) 75, 537, 540, 549, 552, 732, 989, 1084
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 8 cut(s) 76, 538, 541, 550, 553, 733, 990, 1085
Bme1390I CCNGG 3 cut(s) 125, 166, 510
Bme18I GGWCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
BmeT110I CYCGRG 1 cut(s) 387
BmgT120I GGNCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
BmiI GGNNCC 4 cut(s) 325, 425, 426, 649
BmrFI CCNGG 3 cut(s) 125, 166, 510
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 499
BoxI GACNNNNGTC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 187, 546
Bpu1102I GCTNAGC 1 cut(s) 909
BpuMI CCSGG 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 759
BsaJI CCNNGG 2 cut(s) 124, 786
BsaWI WCCGGW 2 cut(s) 218, 802
BsaXI ACNNNNNCTCC 4 cut(s) 231, 261, 1320, 1350
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 1229
BseBI CCWGG 2 cut(s) 125, 166
BseDI CCNNGG 2 cut(s) 124, 786
BseGI GGATG 3 cut(s) 523, 718, 1249
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 1229
BseMII CTCAG 2 cut(s) 900, 1386
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 1 cut(s) 139
BseX3I CGGCCG 1 cut(s) 537
BseXI GCAGC 5 cut(s) 86, 535, 538, 718, 975
BseYI CCCAGC 2 cut(s) 153, 816
BsgI GTGCAG 1 cut(s) 165
Bsh1236I CGCG 1 cut(s) 1377
Bsh1285I CGRYCG 1 cut(s) 540
BshFI GGCC 2 cut(s) 457, 539
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 44, 388
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 3 cut(s) 219, 510, 803
BslFI GGGAC 4 cut(s) 141, 183, 437, 833
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 3 cut(s) 234, 524, 1123
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 4 cut(s) 141, 183, 437, 833
BsmI GAATGC 2 cut(s) 269, 343
BsnI GGCC 2 cut(s) 457, 539
BsoBI CYCGRG 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 44, 388, 978
Bsp143I GATC 3 cut(s) 296, 1223, 1372
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp19I CCATGG 1 cut(s) 786
Bsp68I TCGCGA 1 cut(s) 1377
BspACI CCGC 6 cut(s) 201, 348, 363, 536, 540, 1083
BspANI GGCC 2 cut(s) 457, 539
BspCNI CTCAG 2 cut(s) 901, 1387
BspFNI CGCG 1 cut(s) 1377
BspLI GGNNCC 4 cut(s) 325, 425, 426, 649
BspMAI CTGCAG 2 cut(s) 28, 676
BspPI GGATC 2 cut(s) 304, 1231
BspT107I GGYRCC 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 1111
BsrDI GCAATG 1 cut(s) 1229
BsrI ACTGG 1 cut(s) 494
BssECI CCNNGG 2 cut(s) 124, 786
BssMI GATC 3 cut(s) 296, 1223, 1372
BssNAI GTATAC 1 cut(s) 487
BssNI GRCGYC 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 786
Bst1107I GTATAC 1 cut(s) 487
Bst2UI CCWGG 2 cut(s) 125, 166
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 2 cut(s) 122, 952
BstACI GRCGYC 1 cut(s) 759
BstAFI CTTAAG 1 cut(s) 1111
BstAPI GCANNNNNTGC 2 cut(s) 548, 1445
BstC8I GCNNGC 2 cut(s) 143, 1401
BstDEI CTNAG 4 cut(s) 743, 909, 1321, 1395
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 3 cut(s) 523, 718, 1249
BstFNI CGCG 1 cut(s) 1377
BstKTI GATC 3 cut(s) 299, 1226, 1375
BstMAI GTCTC 3 cut(s) 234, 524, 1123
BstMBI GATC 3 cut(s) 296, 1223, 1372
BstMCI CGRYCG 1 cut(s) 540
BstMWI GCNNNNNNNGC 5 cut(s) 347, 403, 545, 548, 1445
BstNI CCWGG 2 cut(s) 125, 166
BstNSI RCATGY 1 cut(s) 1057
BstPAI GACNNNNGTC 1 cut(s) 133
BstSCI CCNGG 3 cut(s) 123, 164, 508
BstSFI CTRYAG 4 cut(s) 24, 88, 652, 672
BstUI CGCG 1 cut(s) 1377
BstV1I GCAGC 5 cut(s) 86, 535, 538, 718, 975
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 1223
BstYI RGATCY 1 cut(s) 1223
BstZ17I GTATAC 1 cut(s) 487
BstZI CGGCCG 1 cut(s) 537
BsuI GTATCC 2 cut(s) 218, 698
BsuRI GGCC 2 cut(s) 457, 539
BtgI CCRYGG 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 1392
BtsCI GGATG 3 cut(s) 523, 718, 1249
BtsI GCAGTG 2 cut(s) 33, 870
BtsIMutI CAGTG 3 cut(s) 33, 870, 1160
BtuMI TCGCGA 1 cut(s) 1377
Cac8I GCNNGC 2 cut(s) 143, 1401
CciNI GCGGCCGC 1 cut(s) 537
Cfr13I GGNCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
Csp6I GTAC 2 cut(s) 68, 1051
CspCI CAANNNNNGTGG 2 cut(s) 428, 463
CviAII CATG 7 cut(s) 291, 466, 735, 787, 979, 1054, 1177
CviQI GTAC 2 cut(s) 68, 1051
DdeI CTNAG 4 cut(s) 743, 909, 1321, 1395
DpnI GATC 3 cut(s) 298, 1225, 1374
DpnII GATC 3 cut(s) 296, 1223, 1372
EaeI YGGCCR 1 cut(s) 537
EagI CGGCCG 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 122, 952
EarI CTCTTC 2 cut(s) 122, 952
EclXI CGGCCG 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 786
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 978
Eco47I GGWCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
Eco52I CGGCCG 1 cut(s) 537
Eco57I CTGAAG 2 cut(s) 480, 1184
Eco88I CYCGRG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 2 cut(s) 123, 164
EcoT14I CCWWGG 1 cut(s) 786
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 786
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 7 cut(s) 294, 469, 738, 790, 982, 1057, 1180
FaqI GGGAC 4 cut(s) 141, 183, 437, 833
FatI CATG 7 cut(s) 290, 465, 734, 786, 978, 1053, 1176
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 2 cut(s) 435, 486
Fnu4HI GCNGC 8 cut(s) 75, 537, 540, 549, 552, 732, 989, 1084
FokI GGATG 3 cut(s) 530, 725, 1236
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 8 cut(s) 75, 537, 540, 549, 552, 732, 989, 1084
FspBI CTAG 3 cut(s) 45, 501, 719
GluI GCNGC 8 cut(s) 75, 537, 540, 549, 552, 732, 989, 1084
GsaI CCCAGC 2 cut(s) 157, 820
GsuI CTGGAG 2 cut(s) 187, 546
HaeIII GGCC 2 cut(s) 457, 539
HapII CCGG 3 cut(s) 219, 510, 803
Hin1I GRCGYC 1 cut(s) 759
Hin1II CATG 7 cut(s) 294, 469, 738, 790, 982, 1057, 1180
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HinfI GANTC 3 cut(s) 659, 797, 890
HpaII CCGG 3 cut(s) 219, 510, 803
HphI GGTGA 6 cut(s) 265, 489, 792, 806, 933, 1205
Hpy166II GTNNAC 7 cut(s) 61, 211, 436, 487, 577, 1138, 1156
Hpy188I TCNGA 8 cut(s) 190, 356, 517, 569, 927, 1353, 1396, 1430
Hpy188III TCNNGA 4 cut(s) 702, 778, 1367, 1376
Hpy8I GTNNAC 7 cut(s) 61, 211, 436, 487, 577, 1138, 1156
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 6 cut(s) 207, 246, 411, 432, 759, 1073
HpyCH4V TGCA 8 cut(s) 26, 104, 182, 632, 674, 734, 1403, 1448
HpyF10VI GCNNNNNNNGC 5 cut(s) 347, 403, 545, 548, 1445
HpyF3I CTNAG 4 cut(s) 743, 909, 1321, 1395
HpySE526I ACGT 6 cut(s) 207, 246, 411, 432, 759, 1073
Hsp92I GRCGYC 1 cut(s) 759
Hsp92II CATG 7 cut(s) 294, 469, 738, 790, 982, 1057, 1180
KflI GGGWCCC 1 cut(s) 424
Kzo9I GATC 3 cut(s) 296, 1223, 1372
LmnI GCTCC 2 cut(s) 728, 1328
Lsp1109I GCAGC 5 cut(s) 86, 535, 538, 718, 975
LweI GCATC 1 cut(s) 85
MaeI CTAG 3 cut(s) 45, 501, 719
MaeII ACGT 6 cut(s) 207, 246, 411, 432, 759, 1073
MaeIII GTNAC 3 cut(s) 495, 998, 1096
MalI GATC 3 cut(s) 298, 1225, 1374
MboI GATC 3 cut(s) 296, 1223, 1372
MboII GAAGA 7 cut(s) 4, 109, 112, 473, 939, 1177, 1218
MflI RGATCY 1 cut(s) 1223
MhlI GDGCHC 3 cut(s) 44, 388, 978
MluCI AATT 9 cut(s) 159, 175, 251, 633, 772, 827, 884, 914, 1209
MlyI GAGTC 1 cut(s) 884
MmeI TCCRAC 5 cut(s) 905, 1277, 1306, 1318, 1408
MseI TTAA 3 cut(s) 215, 1077, 1112
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 816
MspCI CTTAAG 1 cut(s) 1111
MspI CCGG 3 cut(s) 219, 510, 803
MspR9I CCNGG 3 cut(s) 125, 166, 510
Mva1269I GAATGC 2 cut(s) 269, 343
MvaI CCWGG 2 cut(s) 125, 166
MvnI CGCG 1 cut(s) 1377
MwoI GCNNNNNNNGC 5 cut(s) 347, 403, 545, 548, 1445
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 1 cut(s) 786
NdeII GATC 3 cut(s) 296, 1223, 1372
NlaIII CATG 7 cut(s) 294, 469, 738, 790, 982, 1057, 1180
NlaIV GGNNCC 4 cut(s) 325, 425, 426, 649
NmuCI GTSAC 2 cut(s) 495, 1096
NotI GCGGCCGC 1 cut(s) 537
NruI TCGCGA 1 cut(s) 1377
NspI RCATGY 1 cut(s) 1057
PaeR7I CTCGAG 1 cut(s) 387
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 1053
PctI GAATGC 2 cut(s) 269, 343
PfeI GAWTC 2 cut(s) 659, 797
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 8 cut(s) 76, 538, 541, 550, 553, 733, 990, 1085
PleI GAGTC 1 cut(s) 884
PpsI GAGTC 1 cut(s) 884
PpuMI RGGWCCY 1 cut(s) 424
PscI ACATGT 1 cut(s) 1053
PshAI GACNNNNGTC 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 123, 164
PspFI CCCAGC 2 cut(s) 153, 816
PspGI CCWGG 2 cut(s) 123, 164
PspN4I GGNNCC 4 cut(s) 325, 425, 426, 649
PspPI GGNCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
PspPPI RGGWCCY 1 cut(s) 424
PspXI VCTCGAGB 1 cut(s) 387
PsrI GAACNNNNNNTAC 2 cut(s) 496, 528
PstI CTGCAG 2 cut(s) 28, 676
PsuI RGATCY 1 cut(s) 1223
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 816
RruI TCGCGA 1 cut(s) 1377
RsaI GTAC 2 cut(s) 69, 1052
RsaNI GTAC 2 cut(s) 68, 1051
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 3 cut(s) 215, 1077, 1112
SatI GCNGC 8 cut(s) 75, 537, 540, 549, 552, 732, 989, 1084
Sau3AI GATC 3 cut(s) 296, 1223, 1372
Sau96I GGNCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 3 cut(s) 125, 166, 510
SduI GDGCHC 3 cut(s) 44, 388, 978
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 4 cut(s) 24, 88, 652, 672
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
SlaI CTCGAG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 2 cut(s) 387, 1111
SmoI CTYRAG 2 cut(s) 387, 1111
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 9 cut(s) 159, 175, 251, 633, 772, 827, 884, 914, 1209
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 6 cut(s) 201, 348, 363, 536, 540, 1083
SspMI CTAG 3 cut(s) 45, 501, 719
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 3 cut(s) 123, 164, 508
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 6 cut(s) 210, 249, 414, 435, 762, 1076
TaqI TCGA 2 cut(s) 388, 478
TasI AATT 9 cut(s) 159, 175, 251, 633, 772, 827, 884, 914, 1209
TatI WGTACW 1 cut(s) 1050
TauI GCSGC 3 cut(s) 539, 542, 1086
TfiI GAWTC 2 cut(s) 659, 797
Tru1I TTAA 3 cut(s) 215, 1077, 1112
Tru9I TTAA 3 cut(s) 215, 1077, 1112
TscAI CASTG 3 cut(s) 33, 870, 1167
TseFI GTSAC 2 cut(s) 495, 1096
TseI GCWGC 5 cut(s) 74, 548, 551, 731, 988
Tsp45I GTSAC 2 cut(s) 495, 1096
TspDTI ATGAA 2 cut(s) 1218, 1287
TspRI CASTG 3 cut(s) 33, 870, 1167
Tth111I GACNNNGTC 1 cut(s) 245
Vha464I CTTAAG 1 cut(s) 1111
VpaK11BI GGWCC 6 cut(s) 171, 360, 424, 1190, 1343, 1430
XapI RAATTY 4 cut(s) 251, 827, 884, 1209
XceI RCATGY 1 cut(s) 1057
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XhoI CTCGAG 1 cut(s) 387
XmiI GTMKAC 2 cut(s) 435, 486
XspI CTAG 3 cut(s) 45, 501, 719
ZraI GACGTC 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.