RchiOBHm_Chr6g0251081

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006215
Physical Location & Seq
Reverse (-)
6432221 .. 6434443
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22509

Sequence Viewer

Length: 1497 bp
ATGTCTTGTACTGCTCTGTCTTTCTTCTCCCTCTTCATCTTTGTGCTGATATCACTGGACTCCAGTATTGGTAATGTCCAATTAGTCCAGGCAGGCAACCCAAATTACAGAGATGCCCTTGCAAAGTCCATCCTCTTCTTCCAGGGACAGAGGTCAGGCAACCTCCCCTCCGGCGCCGCCCAACAAATCACTTGGAGGTCTAATTCTGGCCTTTCCGATGGCCGTTTCGCACAAGTGGACTTAACTGGAGGATACTACGATGCTGGAGACAATGTCAAGTTCAACTTCCCAATGGCCTTCACCACCACAATGCTTTCATGGGGAGCACTTGAATATGGCAAGAAAATGGGACCCCAACTAGCAAGTACCCGAGCCGCAATCCGATGGTCCGCAACATATCTTCTAAAATGTGCTAGAGCCACACTCAATAGGCTCTATGTTGGGGTCGGGGACCCCAATATAGACCATAAGTGTTGGGAGAGGCCCGAAGACATGGATACGTCCCGAACCGTTTACTGGGTCTCTCCAAGCAATCCAGGCTCGGATGTTGCTGGAGAGACCGCAGCCGCACTGGCGGCTGCTTCTATGGTTTTTCGAAAGGTAGACTCCAAATATTCGAAGTTGCTATTGAGCACAGCCAAGAAGGTGTTTCAATTTGCAATTCAGTACAGAGGTGCCTACAGTGATTCACTTGGTTCTGCAGTTTGCCCATTTTACTGCTCATATTCTGGATACAAGGATGAGTTGGTGTGGGGTGCTGCATGGCTTCTTAGAGCAACAAATGATGCATTTTACTTCAATTTCTTGAAATCCATGGGAGCTGGTGATTCACCAGACATCTTCAGCTGGGATAACAAATTTGCTGGTGCTTATGTTCTGCTATCAAGGAGGGCTGTGTTGAACAATGACAAGAATTTTGACTCGTATAAACAACAAGCTGAGCAATTTATGTGCGGCATTTTGCCCAACTCACCCTCTTCAAGTACACAATACACACAAGGAGGGCTCATATACAAGCTGCCTGGAAGCAACCTCCAATATGTCACCTCAATTACATTCTTGCTCACTACCTATTCCAAGTACATGTCTGCCAGAAAGCACACATTTAACTGTGGAAACCTTGTCGTCACTTCAAGCGCTTTAAGAAACCTTGCCAAGAGACAGGTGGATTACATATTAGGGGTAAACCCTCTGAAGATGTCCTACATGGTAGGGTATGGACCCTACTATCCCAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCATTGGATAGTCATCCGCAAACGATCGGATGTGATGGCGGTTTCCAGCCGTTCTTCTATTCATCAAATCCAAATCCTAACATCTTAGTTGGAGCTGTTGTTGGAGGTCCAAATCAGAATGATGGGTTTCCAGATGACCGAGCCGATTATAGTCATTCAGAGCCTGCAACATACATAAATGGTGCCATAGTTGGACCATTAGCATACTTTGCTGGGAGCAAATCTCAATAG

Protein Analysis

498

Amino Acids

54.64

Weight (kDa)

9.01

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 36 - 487 8.8e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 173, 674, 1448
AccI GTMKAC 1 cut(s) 603
AciI CCGC 9 cut(s) 177, 375, 390, 561, 567, 575, 954, 1283, 1305
AclWI GGATC 1 cut(s) 1261
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 3 cut(s) 857, 913, 1239
AcuI CTGAAG 2 cut(s) 826, 1214
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 5 cut(s) 10, 367, 668, 985, 1082
AfeI AGCGCT 1 cut(s) 1138
AfiI CCNNNNNNNGG 1 cut(s) 516
AflIII ACRYGT 1 cut(s) 1083
AgsI TTSAA 8 cut(s) 283, 332, 653, 799, 808, 901, 981, 1134
AjnI CCWGG 4 cut(s) 87, 141, 535, 1021
AluBI AGCT 5 cut(s) 821, 846, 938, 1018, 1361
AluI AGCT 5 cut(s) 821, 846, 938, 1018, 1361
Alw21I GWGCWC 2 cut(s) 328, 635
Alw26I GTCTC 4 cut(s) 261, 526, 551, 1153
AlwI GGATC 1 cut(s) 1261
AlwNI CAGNNNCTG 1 cut(s) 1430
Ama87I CYCGRG 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 1138
AoxI GGCC 4 cut(s) 208, 220, 294, 482
ApeKI GCWGC 4 cut(s) 563, 578, 758, 1018
ApoI RAATTY 3 cut(s) 857, 913, 1239
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AspLEI GCGC 2 cut(s) 176, 1139
AspS9I GGNCC 7 cut(s) 350, 387, 451, 483, 1220, 1373, 1460
AsuHPI GGTGA 6 cut(s) 292, 822, 836, 963, 1036, 1235
AsuII TTCGAA 2 cut(s) 595, 617
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 6 cut(s) 350, 387, 451, 1220, 1373, 1460
BanI GGYRCC 3 cut(s) 173, 674, 1448
BanII GRGCYC 1 cut(s) 1008
BarI GAAGNNNNNNTAC 2 cut(s) 1187, 1219
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 328, 635
BbvI GCAGC 4 cut(s) 565, 575, 745, 1005
BccI CCATC 5 cut(s) 137, 212, 378, 1295, 1382
BceAI ACGGC 2 cut(s) 207, 1300
BciT130I CCWGG 4 cut(s) 89, 143, 537, 1023
BciVI GTATCC 3 cut(s) 245, 490, 725
BcoDI GTCTC 4 cut(s) 261, 526, 551, 1153
BfaI CTAG 2 cut(s) 359, 414
BfmI CTRYAG 2 cut(s) 679, 699
BfoI RGCGCY 2 cut(s) 177, 1140
BfuI GTATCC 3 cut(s) 245, 490, 725
BglI GCCNNNNNGGC 1 cut(s) 572
BisI GCNGC 9 cut(s) 177, 375, 564, 567, 576, 579, 759, 955, 1019
BlpI GCTNAGC 1 cut(s) 939
BlsI GCNGC 9 cut(s) 178, 376, 565, 568, 577, 580, 760, 956, 1020
Bme1390I CCNGG 4 cut(s) 89, 143, 537, 1023
Bme18I GGWCC 6 cut(s) 350, 387, 451, 1220, 1373, 1460
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 7 cut(s) 350, 387, 451, 483, 1220, 1373, 1460
BmiI GGNNCC 8 cut(s) 175, 351, 352, 452, 453, 676, 1222, 1450
BmrFI CCNGG 4 cut(s) 89, 143, 537, 1023
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 3 cut(s) 103, 250, 775
BmuI ACTGGG 1 cut(s) 526
BoxI GACNNNNGTC 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 495
BplI GAGNNNNNCTC 3 cut(s) 408, 440, 1474
BpmI CTGGAG 4 cut(s) 46, 267, 285, 573
Bpu1102I GCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 2 cut(s) 595, 617
BsaBI GATNNNNATC 1 cut(s) 1278
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 2 cut(s) 526, 551
BsaJI CCNNGG 2 cut(s) 142, 813
BsaXI ACNNNNNCTCC 6 cut(s) 152, 182, 258, 288, 1350, 1380
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 5 cut(s) 60, 63, 250, 521, 576
Bse3DI GCAATG 1 cut(s) 1259
Bse8I GATNNNNATC 1 cut(s) 1278
BseBI CCWGG 4 cut(s) 89, 143, 537, 1023
BseDI CCNNGG 2 cut(s) 142, 813
BseGI GGATG 5 cut(s) 129, 550, 745, 1279, 1301
BseJI GATNNNNATC 1 cut(s) 1278
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 1259
BseMII CTCAG 1 cut(s) 930
BseNI ACTGG 5 cut(s) 60, 63, 250, 521, 576
BseXI GCAGC 4 cut(s) 565, 575, 745, 1005
BseYI CCCAGC 2 cut(s) 846, 1478
Bsh1285I CGRYCG 1 cut(s) 1293
BshFI GGCC 4 cut(s) 210, 222, 296, 484
BshNI GGYRCC 3 cut(s) 173, 674, 1448
BsiEI CGRYCG 1 cut(s) 1293
BsiHKAI GWGCWC 2 cut(s) 328, 635
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 4 cut(s) 159, 363, 464, 487
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 4 cut(s) 261, 526, 551, 1153
BsmFI GGGAC 4 cut(s) 159, 363, 464, 487
BsnI GGCC 4 cut(s) 210, 222, 296, 484
Bso31I GGTCTC 2 cut(s) 526, 551
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 2 cut(s) 595, 617
Bsp1286I GDGCHC 3 cut(s) 328, 635, 1008
Bsp143I GATC 2 cut(s) 1253, 1290
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 813
BspACI CCGC 9 cut(s) 177, 375, 390, 561, 567, 575, 954, 1283, 1305
BspANI GGCC 4 cut(s) 210, 222, 296, 484
BspCNI CTCAG 1 cut(s) 931
BspLI GGNNCC 8 cut(s) 175, 351, 352, 452, 453, 676, 1222, 1450
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 1 cut(s) 1261
BspT104I TTCGAA 2 cut(s) 595, 617
BspT107I GGYRCC 3 cut(s) 173, 674, 1448
BspTNI GGTCTC 2 cut(s) 526, 551
BsrDI GCAATG 1 cut(s) 1259
BsrI ACTGG 5 cut(s) 60, 63, 250, 521, 576
BssECI CCNNGG 2 cut(s) 142, 813
BssMI GATC 2 cut(s) 1253, 1290
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 4 cut(s) 89, 143, 537, 1023
Bst4CI ACNGT 3 cut(s) 511, 683, 1112
Bst6I CTCTTC 3 cut(s) 38, 140, 982
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstBI TTCGAA 2 cut(s) 595, 617
BstC8I GCNNGC 2 cut(s) 94, 1431
BstDEI CTNAG 3 cut(s) 770, 939, 1351
BstDSI CCRYGG 1 cut(s) 813
BstF5I GGATG 5 cut(s) 129, 550, 745, 1279, 1301
BstH2I RGCGCY 2 cut(s) 177, 1140
BstHHI GCGC 2 cut(s) 176, 1139
BstKTI GATC 2 cut(s) 1256, 1293
BstMAI GTCTC 4 cut(s) 261, 526, 551, 1153
BstMBI GATC 2 cut(s) 1253, 1290
BstMCI CGRYCG 1 cut(s) 1293
BstMWI GCNNNNNNNGC 4 cut(s) 537, 572, 575, 1475
BstNI CCWGG 4 cut(s) 89, 143, 537, 1023
BstNSI RCATGY 1 cut(s) 1087
BstPAI GACNNNNGTC 1 cut(s) 151
BstSCI CCNGG 4 cut(s) 87, 141, 535, 1021
BstSFI CTRYAG 2 cut(s) 679, 699
BstV1I GCAGC 4 cut(s) 565, 575, 745, 1005
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 1253
BstYI RGATCY 1 cut(s) 1253
BsuI GTATCC 3 cut(s) 245, 490, 725
BsuRI GGCC 4 cut(s) 210, 222, 296, 484
BtgI CCRYGG 1 cut(s) 813
BtsCI GGATG 5 cut(s) 129, 550, 745, 1279, 1301
BtsIMutI CAGTG 3 cut(s) 53, 569, 688
Cac8I GCNNGC 2 cut(s) 94, 1431
CaiI CAGNNNCTG 1 cut(s) 1430
CfoI GCGC 2 cut(s) 176, 1139
Cfr13I GGNCC 7 cut(s) 350, 387, 451, 483, 1220, 1373, 1460
Csp6I GTAC 5 cut(s) 9, 366, 667, 984, 1081
CviAII CATG 6 cut(s) 318, 493, 762, 814, 1084, 1207
CviQI GTAC 5 cut(s) 9, 366, 667, 984, 1081
DdeI CTNAG 3 cut(s) 770, 939, 1351
DinI GGCGCC 1 cut(s) 175
DpnI GATC 2 cut(s) 1255, 1292
DpnII GATC 2 cut(s) 1253, 1290
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 3 cut(s) 38, 140, 982
EarI CTCTTC 3 cut(s) 38, 140, 982
Eco130I CCWWGG 1 cut(s) 813
Eco24I GRGCYC 1 cut(s) 1008
Eco31I GGTCTC 2 cut(s) 526, 551
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 6 cut(s) 350, 387, 451, 1220, 1373, 1460
Eco47III AGCGCT 1 cut(s) 1138
Eco57I CTGAAG 2 cut(s) 826, 1214
Eco88I CYCGRG 1 cut(s) 369
EcoO109I RGGNCCY 2 cut(s) 350, 451
EcoRI GAATTC 1 cut(s) 1239
EcoRII CCWGG 4 cut(s) 87, 141, 535, 1021
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 813
EcoT22I ATGCAT 1 cut(s) 790
EcoT38I GRGCYC 1 cut(s) 1008
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 6 cut(s) 321, 496, 765, 817, 1087, 1210
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 4 cut(s) 159, 363, 464, 487
FatI CATG 6 cut(s) 317, 492, 761, 813, 1083, 1206
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 9 cut(s) 177, 375, 564, 567, 576, 579, 759, 955, 1019
FokI GGATG 5 cut(s) 116, 557, 752, 1266, 1308
FriOI GRGCYC 1 cut(s) 1008
Fsp4HI GCNGC 9 cut(s) 177, 375, 564, 567, 576, 579, 759, 955, 1019
FspBI CTAG 2 cut(s) 359, 414
GlaI GCGC 2 cut(s) 175, 1138
GluI GCNGC 9 cut(s) 177, 375, 564, 567, 576, 579, 759, 955, 1019
GsaI CCCAGC 2 cut(s) 850, 1482
GsuI CTGGAG 4 cut(s) 46, 267, 285, 573
HaeII RGCGCY 2 cut(s) 177, 1140
HaeIII GGCC 4 cut(s) 210, 222, 296, 484
HapII CCGG 1 cut(s) 171
HhaI GCGC 2 cut(s) 176, 1139
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 321, 496, 765, 817, 1087, 1210
Hin6I GCGC 2 cut(s) 174, 1137
HinP1I GCGC 2 cut(s) 174, 1137
HinfI GANTC 5 cut(s) 59, 605, 686, 827, 920
HpaII CCGG 1 cut(s) 171
HphI GGTGA 6 cut(s) 292, 822, 836, 963, 1036, 1235
Hpy166II GTNNAC 5 cut(s) 238, 514, 604, 986, 1186
Hpy188I TCNGA 7 cut(s) 217, 383, 544, 1194, 1295, 1383, 1426
Hpy188III TCNNGA 4 cut(s) 504, 729, 805, 1397
Hpy8I GTNNAC 5 cut(s) 238, 514, 604, 986, 1186
HpyAV CCTTC 2 cut(s) 307, 637
HpyCH4III ACNGT 3 cut(s) 511, 683, 1112
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 6 cut(s) 122, 659, 701, 761, 788, 1433
HpyF10VI GCNNNNNNNGC 4 cut(s) 537, 572, 575, 1475
HpyF3I CTNAG 3 cut(s) 770, 939, 1351
HpySE526I ACGT 1 cut(s) 500
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 321, 496, 765, 817, 1087, 1210
HspAI GCGC 2 cut(s) 174, 1137
KasI GGCGCC 1 cut(s) 173
KflI GGGWCCC 2 cut(s) 350, 451
Kzo9I GATC 2 cut(s) 1253, 1290
LmnI GCTCC 4 cut(s) 323, 818, 1358, 1482
Lsp1109I GCAGC 4 cut(s) 565, 575, 745, 1005
LweI GCATC 3 cut(s) 103, 250, 775
MaeI CTAG 2 cut(s) 359, 414
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 2 cut(s) 1042, 1126
MalI GATC 2 cut(s) 1255, 1292
MboI GATC 2 cut(s) 1253, 1290
MflI RGATCY 1 cut(s) 1253
MhlI GDGCHC 3 cut(s) 328, 635, 1008
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 3 cut(s) 53, 599, 914
MmeI TCCRAC 3 cut(s) 1336, 1348, 1438
Mph1103I ATGCAT 1 cut(s) 790
MseI TTAA 3 cut(s) 242, 1107, 1142
MslI CAYNNNNRTG 2 cut(s) 41, 308
MspA1I CMGCKG 1 cut(s) 846
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 4 cut(s) 89, 143, 537, 1023
MvaI CCWGG 4 cut(s) 89, 143, 537, 1023
MwoI GCNNNNNNNGC 4 cut(s) 537, 572, 575, 1475
NarI GGCGCC 1 cut(s) 174
NcoI CCATGG 1 cut(s) 813
NdeII GATC 2 cut(s) 1253, 1290
NlaIII CATG 6 cut(s) 321, 496, 765, 817, 1087, 1210
NlaIV GGNNCC 8 cut(s) 175, 351, 352, 452, 453, 676, 1222, 1450
NmuCI GTSAC 2 cut(s) 1042, 1126
NsiI ATGCAT 1 cut(s) 790
NspI RCATGY 1 cut(s) 1087
NspV TTCGAA 2 cut(s) 595, 617
PciI ACATGT 1 cut(s) 1083
PfeI GAWTC 2 cut(s) 686, 827
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 9 cut(s) 178, 376, 565, 568, 577, 580, 760, 956, 1020
Ple19I CGATCG 1 cut(s) 1293
PleI GAGTC 3 cut(s) 53, 599, 914
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 3 cut(s) 53, 599, 914
PpuMI RGGWCCY 2 cut(s) 350, 451
PscI ACATGT 1 cut(s) 1083
PshAI GACNNNNGTC 1 cut(s) 151
Psp5II RGGWCCY 2 cut(s) 350, 451
Psp6I CCWGG 4 cut(s) 87, 141, 535, 1021
PspFI CCCAGC 2 cut(s) 846, 1478
PspGI CCWGG 4 cut(s) 87, 141, 535, 1021
PspN4I GGNNCC 8 cut(s) 175, 351, 352, 452, 453, 676, 1222, 1450
PspPI GGNCC 7 cut(s) 350, 387, 451, 483, 1220, 1373, 1460
PspPPI RGGWCCY 2 cut(s) 350, 451
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 1 cut(s) 1253
PsyI GACNNNGTC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 1293
PvuII CAGCTG 1 cut(s) 846
RsaI GTAC 5 cut(s) 10, 367, 668, 985, 1082
RsaNI GTAC 5 cut(s) 9, 366, 667, 984, 1081
RseI CAYNNNNRTG 2 cut(s) 41, 308
SaqAI TTAA 3 cut(s) 242, 1107, 1142
SatI GCNGC 9 cut(s) 177, 375, 564, 567, 576, 579, 759, 955, 1019
Sau3AI GATC 2 cut(s) 1253, 1290
Sau96I GGNCC 7 cut(s) 350, 387, 451, 483, 1220, 1373, 1460
SchI GAGTC 3 cut(s) 53, 599, 914
ScrFI CCNGG 4 cut(s) 89, 143, 537, 1023
SduI GDGCHC 3 cut(s) 328, 635, 1008
SfaNI GCATC 3 cut(s) 103, 250, 775
SfcI CTRYAG 2 cut(s) 679, 699
SfoI GGCGCC 1 cut(s) 175
SfuI TTCGAA 2 cut(s) 595, 617
SinI GGWCC 6 cut(s) 350, 387, 451, 1220, 1373, 1460
SmiMI CAYNNNNRTG 2 cut(s) 41, 308
SsiI CCGC 9 cut(s) 177, 375, 390, 561, 567, 575, 954, 1283, 1305
SspDI GGCGCC 1 cut(s) 173
SspI AATATT 1 cut(s) 614
SspMI CTAG 2 cut(s) 359, 414
StyD4I CCNGG 4 cut(s) 87, 141, 535, 1021
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 3 cut(s) 511, 683, 1112
TaiI ACGT 1 cut(s) 503
TaqI TCGA 2 cut(s) 595, 617
TaqII GACCGA 1 cut(s) 1419
TatI WGTACW 4 cut(s) 8, 666, 983, 1080
TauI GCSGC 5 cut(s) 179, 377, 569, 578, 957
TfiI GAWTC 2 cut(s) 686, 827
Tru1I TTAA 3 cut(s) 242, 1107, 1142
Tru9I TTAA 3 cut(s) 242, 1107, 1142
TscAI CASTG 3 cut(s) 60, 576, 688
TseFI GTSAC 2 cut(s) 1042, 1126
TseI GCWGC 4 cut(s) 563, 578, 758, 1018
Tsp45I GTSAC 2 cut(s) 1042, 1126
TspDTI ATGAA 4 cut(s) 25, 306, 1248, 1317
TspRI CASTG 3 cut(s) 60, 576, 688
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 6 cut(s) 350, 387, 451, 1220, 1373, 1460
XapI RAATTY 3 cut(s) 857, 913, 1239
XceI RCATGY 1 cut(s) 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 2 cut(s) 359, 414
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.