Rh6DG005800

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006215
Physical Location & Seq
Reverse (-)
656381 .. 659250
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG005800.1

Sequence Viewer

Length: 1149 bp
ATGGCCTTCACCACCACAATGCTTTCATGGGGAGCACTTGAATATGGCAAGAAAATGGGACCCCAACTAGCAAGTACCCGGGCCGCAATCCGATGGTCCGCAACATATCTTCTAAAATGTGCTAGAGCCACACCCAATAGGCTCTATGTTGGGGTCGGGGACCCCAATATAGACCACAAGTGTTGGGAGAGGCCCGAAGACATGGATACGTCCCGAACCGTTTACTGGGTCTCTCCAAGCAATCCAGGCTCGGATGTTGCTGGAGAGACGGCAGCTGCACTGGCGGCTGCTTCTATGGTTTTTCGAAAGGTAGACTCCAAATATTCGAAGTTGCTATTGAGCACAGCCAAGAAGGTGTTTCAATTTGCAATTCAGTACAGAGGTGCCTACAGTGATTCACTTGGTTCTGCAGTTTGCCCATTTTACTGCTCATATTCTGGATACAAGGATGAGTTGGTGTGGGGTGCAGCATGGCTTCTTAGAGCAACAAATGATGCTTTTTACTTCAATTTCTTGAAATCCATGGGAGCTGGTGATTCACCAGACATCTTCAGCTGGGATAACAAATTTGCTGGTGCTTATGTTCTGCTATCAAGGAGGGCTGTGTTGAACAATGACAAGAATTTTGACTCGTATAAACAACAAGCTGAGCAATTTATGTGCGGCATTTTGCCCAACTCACCCTCTTCAAGTACACAATACACACAAGGAGGGCTCATATACAAGCTGCCTGGAAGTAACCTCCAATATGTCACCTCAATTACATTCTTGCTCACTACCTATTCCAAGTACATGTCTGCCAGAAAGCACACATTTAACTGTGGAAACCTTGTCGTCACTTCAAGCGCTTTAAGAAACCTTGCCAAGAGACAGGTGGATTACATATTAGGGGTAAACCCTCTGAAGATGTCCTACATGGTAGGGTATGGACCCTACTATCCCAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCATTGGATAGTCATCCGCAAACGATCGGATGTGATGGCGGTTTCCAACCGTTCTTCTATTCATCAAATCCAAATCCTAACATCTTAGTTGGAGCTGTTGTTGGAGGTCCAAATCAGAATGATGGGTTTCCAGATGACCGAGCCGATTATAGTCATTCAGAGCCTGCAACATAA

Protein Analysis

382

Amino Acids

42.21

Weight (kDa)

9.03

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 382 7.6e-124 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 1 cut(s) 312
AciI CCGC 6 cut(s) 84, 99, 284, 663, 992, 1014
AclWI GGATC 1 cut(s) 970
AcsI RAATTY 3 cut(s) 566, 622, 948
AcuI CTGAAG 2 cut(s) 535, 923
AfaI GTAC 4 cut(s) 76, 377, 694, 791
AfeI AGCGCT 1 cut(s) 847
AfiI CCNNNNNNNGG 1 cut(s) 225
AflIII ACRYGT 1 cut(s) 792
AgsI TTSAA 7 cut(s) 41, 362, 508, 517, 610, 690, 843
AjnI CCWGG 2 cut(s) 244, 730
AluBI AGCT 6 cut(s) 275, 530, 555, 647, 727, 1070
AluI AGCT 6 cut(s) 275, 530, 555, 647, 727, 1070
Alw21I GWGCWC 2 cut(s) 37, 344
Alw26I GTCTC 3 cut(s) 235, 260, 862
AlwI GGATC 1 cut(s) 970
AlwNI CAGNNNCTG 1 cut(s) 1139
Ama87I CYCGRG 1 cut(s) 78
Aor51HI AGCGCT 1 cut(s) 847
AoxI GGCC 3 cut(s) 3, 81, 191
ApeKI GCWGC 5 cut(s) 272, 275, 287, 467, 727
ApoI RAATTY 3 cut(s) 566, 622, 948
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AspLEI GCGC 1 cut(s) 848
AspS9I GGNCC 7 cut(s) 59, 81, 96, 160, 192, 929, 1082
AsuC2I CCSGG 2 cut(s) 79, 80
AsuHPI GGTGA 5 cut(s) 531, 545, 672, 745, 944
AsuII TTCGAA 2 cut(s) 304, 326
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 5 cut(s) 59, 96, 160, 929, 1082
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 717
BarI GAAGNNNNNNTAC 2 cut(s) 896, 928
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 37, 344
BbvI GCAGC 5 cut(s) 262, 274, 284, 479, 714
BccI CCATC 3 cut(s) 87, 1004, 1091
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 2 cut(s) 246, 732
BciVI GTATCC 2 cut(s) 199, 434
BcnI CCSGG 2 cut(s) 79, 80
BcoDI GTCTC 3 cut(s) 235, 260, 862
BfaI CTAG 2 cut(s) 68, 123
BfmI CTRYAG 2 cut(s) 388, 408
BfoI RGCGCY 1 cut(s) 849
BfuI GTATCC 2 cut(s) 199, 434
BisI GCNGC 8 cut(s) 84, 273, 276, 285, 288, 468, 664, 728
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 8 cut(s) 85, 274, 277, 286, 289, 469, 665, 729
Bme1390I CCNGG 4 cut(s) 79, 80, 246, 732
Bme18I GGWCC 5 cut(s) 59, 96, 160, 929, 1082
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 7 cut(s) 59, 81, 96, 160, 192, 929, 1082
BmiI GGNNCC 6 cut(s) 60, 61, 161, 162, 385, 931
BmrFI CCNGG 4 cut(s) 79, 80, 246, 732
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 484
BmuI ACTGGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 2 cut(s) 304, 326
BpuMI CCSGG 2 cut(s) 79, 80
BsaBI GATNNNNATC 1 cut(s) 987
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 78, 522
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 230, 285
Bse3DI GCAATG 1 cut(s) 968
Bse8I GATNNNNATC 1 cut(s) 987
BseBI CCWGG 2 cut(s) 246, 732
BseDI CCNNGG 2 cut(s) 78, 522
BseGI GGATG 4 cut(s) 259, 454, 988, 1010
BseJI GATNNNNATC 1 cut(s) 987
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 968
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 2 cut(s) 230, 285
BseXI GCAGC 5 cut(s) 262, 274, 284, 479, 714
BseYI CCCAGC 1 cut(s) 555
BsgI GTGCAG 2 cut(s) 261, 486
Bsh1285I CGRYCG 1 cut(s) 1002
BshFI GGCC 3 cut(s) 5, 83, 193
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 1002
BsiHKAI GWGCWC 2 cut(s) 37, 344
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 3 cut(s) 72, 173, 196
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 3 cut(s) 235, 260, 862
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 3 cut(s) 72, 173, 196
BsnI GGCC 3 cut(s) 5, 83, 193
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 2 cut(s) 304, 326
Bsp1286I GDGCHC 3 cut(s) 37, 344, 717
Bsp143I GATC 2 cut(s) 962, 999
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 6 cut(s) 84, 99, 284, 663, 992, 1014
BspANI GGCC 3 cut(s) 5, 83, 193
BspCNI CTCAG 1 cut(s) 640
BspLI GGNNCC 6 cut(s) 60, 61, 161, 162, 385, 931
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 970
BspT104I TTCGAA 2 cut(s) 304, 326
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 968
BsrI ACTGG 2 cut(s) 230, 285
BssECI CCNNGG 2 cut(s) 78, 522
BssMI GATC 2 cut(s) 962, 999
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 246, 732
Bst4CI ACNGT 4 cut(s) 220, 392, 821, 1026
Bst6I CTCTTC 1 cut(s) 691
BstBI TTCGAA 2 cut(s) 304, 326
BstC8I GCNNGC 1 cut(s) 1140
BstDEI CTNAG 3 cut(s) 479, 648, 1060
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 259, 454, 988, 1010
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 1 cut(s) 848
BstKTI GATC 2 cut(s) 965, 1002
BstMAI GTCTC 3 cut(s) 235, 260, 862
BstMBI GATC 2 cut(s) 962, 999
BstMCI CGRYCG 1 cut(s) 1002
BstMWI GCNNNNNNNGC 3 cut(s) 246, 281, 284
BstNI CCWGG 2 cut(s) 246, 732
BstNSI RCATGY 1 cut(s) 796
BstSCI CCNGG 4 cut(s) 77, 78, 244, 730
BstSFI CTRYAG 2 cut(s) 388, 408
BstV1I GCAGC 5 cut(s) 262, 274, 284, 479, 714
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
BsuI GTATCC 2 cut(s) 199, 434
BsuRI GGCC 3 cut(s) 5, 83, 193
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 4 cut(s) 259, 454, 988, 1010
BtsIMutI CAGTG 2 cut(s) 278, 397
Cac8I GCNNGC 1 cut(s) 1140
CaiI CAGNNNCTG 1 cut(s) 1139
CfoI GCGC 1 cut(s) 848
Cfr13I GGNCC 7 cut(s) 59, 81, 96, 160, 192, 929, 1082
Cfr9I CCCGGG 1 cut(s) 78
Csp6I GTAC 4 cut(s) 75, 376, 693, 790
CspCI CAANNNNNGTGG 2 cut(s) 164, 199
CviAII CATG 6 cut(s) 27, 202, 471, 523, 793, 916
CviQI GTAC 4 cut(s) 75, 376, 693, 790
DdeI CTNAG 3 cut(s) 479, 648, 1060
DpnI GATC 2 cut(s) 964, 1001
DpnII GATC 2 cut(s) 962, 999
Eam1104I CTCTTC 1 cut(s) 691
EarI CTCTTC 1 cut(s) 691
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 5 cut(s) 59, 96, 160, 929, 1082
Eco47III AGCGCT 1 cut(s) 847
Eco57I CTGAAG 2 cut(s) 535, 923
Eco88I CYCGRG 1 cut(s) 78
EcoO109I RGGNCCY 2 cut(s) 59, 160
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 2 cut(s) 244, 730
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 6 cut(s) 30, 205, 474, 526, 796, 919
FaqI GGGAC 3 cut(s) 72, 173, 196
FatI CATG 6 cut(s) 26, 201, 470, 522, 792, 915
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 8 cut(s) 84, 273, 276, 285, 288, 468, 664, 728
FokI GGATG 4 cut(s) 266, 461, 975, 1017
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 8 cut(s) 84, 273, 276, 285, 288, 468, 664, 728
FspBI CTAG 2 cut(s) 68, 123
GlaI GCGC 1 cut(s) 847
GluI GCNGC 8 cut(s) 84, 273, 276, 285, 288, 468, 664, 728
GsaI CCCAGC 1 cut(s) 559
GsuI CTGGAG 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 3 cut(s) 5, 83, 193
HapII CCGG 1 cut(s) 79
HhaI GCGC 1 cut(s) 848
Hin1II CATG 6 cut(s) 30, 205, 474, 526, 796, 919
Hin6I GCGC 1 cut(s) 846
HinP1I GCGC 1 cut(s) 846
HinfI GANTC 4 cut(s) 314, 395, 536, 629
HpaII CCGG 1 cut(s) 79
HphI GGTGA 5 cut(s) 531, 545, 672, 745, 944
Hpy166II GTNNAC 4 cut(s) 223, 313, 695, 895
Hpy188I TCNGA 6 cut(s) 92, 253, 903, 1004, 1092, 1135
Hpy188III TCNNGA 4 cut(s) 213, 438, 514, 1106
Hpy8I GTNNAC 4 cut(s) 223, 313, 695, 895
HpyAV CCTTC 2 cut(s) 16, 346
HpyCH4III ACNGT 4 cut(s) 220, 392, 821, 1026
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 5 cut(s) 278, 368, 410, 467, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 281, 284
HpyF3I CTNAG 3 cut(s) 479, 648, 1060
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 6 cut(s) 30, 205, 474, 526, 796, 919
HspAI GCGC 1 cut(s) 846
KflI GGGWCCC 2 cut(s) 59, 160
Kzo9I GATC 2 cut(s) 962, 999
LmnI GCTCC 3 cut(s) 32, 527, 1067
Lsp1109I GCAGC 5 cut(s) 262, 274, 284, 479, 714
LweI GCATC 1 cut(s) 484
MaeI CTAG 2 cut(s) 68, 123
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 3 cut(s) 737, 751, 835
MalI GATC 2 cut(s) 964, 1001
MboI GATC 2 cut(s) 962, 999
MboII GAAGA 7 cut(s) 101, 209, 541, 678, 916, 957, 1021
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 3 cut(s) 37, 344, 717
MluCI AATT 8 cut(s) 362, 369, 508, 566, 622, 653, 759, 948
MlyI GAGTC 2 cut(s) 308, 623
MmeI TCCRAC 3 cut(s) 1045, 1045, 1057
MseI TTAA 2 cut(s) 816, 851
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 275, 555
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 4 cut(s) 79, 80, 246, 732
MvaI CCWGG 2 cut(s) 246, 732
MwoI GCNNNNNNNGC 3 cut(s) 246, 281, 284
NciI CCSGG 2 cut(s) 79, 80
NcoI CCATGG 1 cut(s) 522
NdeII GATC 2 cut(s) 962, 999
NlaIII CATG 6 cut(s) 30, 205, 474, 526, 796, 919
NlaIV GGNNCC 6 cut(s) 60, 61, 161, 162, 385, 931
NmuCI GTSAC 2 cut(s) 751, 835
NspI RCATGY 1 cut(s) 796
NspV TTCGAA 2 cut(s) 304, 326
PciI ACATGT 1 cut(s) 792
PfeI GAWTC 2 cut(s) 395, 536
PkrI GCNGC 8 cut(s) 85, 274, 277, 286, 289, 469, 665, 729
Ple19I CGATCG 1 cut(s) 1002
PleI GAGTC 2 cut(s) 308, 623
PpsI GAGTC 2 cut(s) 308, 623
PpuMI RGGWCCY 2 cut(s) 59, 160
PscI ACATGT 1 cut(s) 792
Psp5II RGGWCCY 2 cut(s) 59, 160
Psp6I CCWGG 2 cut(s) 244, 730
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 244, 730
PspN4I GGNNCC 6 cut(s) 60, 61, 161, 162, 385, 931
PspPI GGNCC 7 cut(s) 59, 81, 96, 160, 192, 929, 1082
PspPPI RGGWCCY 2 cut(s) 59, 160
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 1139
PsuI RGATCY 1 cut(s) 962
PvuI CGATCG 1 cut(s) 1002
PvuII CAGCTG 2 cut(s) 275, 555
RsaI GTAC 4 cut(s) 76, 377, 694, 791
RsaNI GTAC 4 cut(s) 75, 376, 693, 790
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 2 cut(s) 816, 851
SatI GCNGC 8 cut(s) 84, 273, 276, 285, 288, 468, 664, 728
Sau3AI GATC 2 cut(s) 962, 999
Sau96I GGNCC 7 cut(s) 59, 81, 96, 160, 192, 929, 1082
SchI GAGTC 2 cut(s) 308, 623
ScrFI CCNGG 4 cut(s) 79, 80, 246, 732
SduI GDGCHC 3 cut(s) 37, 344, 717
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 2 cut(s) 388, 408
SfuI TTCGAA 2 cut(s) 304, 326
SinI GGWCC 5 cut(s) 59, 96, 160, 929, 1082
SmaI CCCGGG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 17
Sse9I AATT 8 cut(s) 362, 369, 508, 566, 622, 653, 759, 948
SsiI CCGC 6 cut(s) 84, 99, 284, 663, 992, 1014
SspI AATATT 1 cut(s) 323
SspMI CTAG 2 cut(s) 68, 123
StyD4I CCNGG 4 cut(s) 77, 78, 244, 730
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 4 cut(s) 220, 392, 821, 1026
TaiI ACGT 1 cut(s) 212
TaqI TCGA 2 cut(s) 304, 326
TaqII GACCGA 1 cut(s) 1128
TasI AATT 8 cut(s) 362, 369, 508, 566, 622, 653, 759, 948
TatI WGTACW 3 cut(s) 375, 692, 789
TauI GCSGC 3 cut(s) 86, 287, 666
TfiI GAWTC 2 cut(s) 395, 536
Tru1I TTAA 2 cut(s) 816, 851
Tru9I TTAA 2 cut(s) 816, 851
TscAI CASTG 2 cut(s) 285, 397
TseFI GTSAC 2 cut(s) 751, 835
TseI GCWGC 5 cut(s) 272, 275, 287, 467, 727
Tsp45I GTSAC 2 cut(s) 751, 835
TspDTI ATGAA 3 cut(s) 15, 957, 1026
TspMI CCCGGG 1 cut(s) 78
TspRI CASTG 2 cut(s) 285, 397
VpaK11BI GGWCC 5 cut(s) 59, 96, 160, 929, 1082
XapI RAATTY 3 cut(s) 566, 622, 948
XceI RCATGY 1 cut(s) 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XmaI CCCGGG 1 cut(s) 78
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 2 cut(s) 68, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.