MD05G1002700.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006215
Physical Location & Seq
Reverse (-)
1042179 .. 1046269
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1002700.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGCAAAGAGATTACCACTAACCCTCTTCATCTCTGTCGTTGTAATATTGGGCAGTAGTATTAGTAGTAGTGTCCAAATCCAAGCTGTAGGCAACCCTAATTACAGAGAGGCCCTGGCAAAGTCCATACTCTTCTTTCAGGGACAAAGGTCAGGGAGACTCCCCGCCGGTGCTGCCCAACAAATCACATGGAGGTTCAATTCTGGGCTTTCCGATGGTCGTCTGGCAAATGTGGACTTAACTGGAGGATACTATGACGCTGGAGACAATGTCAAGTTCAACTTCCCAATGGCATTCACCACCACAATGCTGTCATGGGGAGCACTTGAATATGGCAAGCGCATTGGCCTCCAGTTACCAGAAGCCCGGGCAGCGATACGGTGGGCCACGGATTATCTTCTAAAGTGTGCTAGGCAAACGCCCGGTAAACTCTATGTTGGGGTCGGTGATCCAAATGTGGACCATAAATGTTGGGAGAGGCCAGAAGACATGGACACGCTTAGAACCGTGTACTCAGTCTCACCAAGCAACCCGGGCTCGGACGTTGCTGGAGAGACAGCCGCTGCATTAGCGGCTGCTTCTTTAGTTTTCCGGAAGGTTGATCCGAAATACTCAAAGTTGCTGTTGAACACGGCCAAGAATGTGATGCAGTTTGCAATTCAGTACCGAGGGGCATACAGTGATTCACTTGGATCTGCAGTCTGCCCATTTTATTGCTCATATTCTGGATATAAGGATGAGCTGCTGTGGGGAGCTGCATGGCTTTTTAGAGCAAGTAATGAACTTTATTATTACAATTTTATGAAATCCTTGGGAGCTAGTGATTCAACAGACATTTTCAGCTGGGACAACAAATTTGCTGGTGCTTATGTTTTATTATCAAGGAGGGCACTGTTGAACAACGACAAAAACTTTGAGCCATATAAACAACAAGCAGAGCAATTTATGTGCCGGATTTTGCCTAACTCACCCTCTTCAAGTACTCAATATACACAAGGAGGGCTCATATATAAGCTGCCTGGAAGTAACCTCCAATATGTGACCTCAATAACATTCTTGCTTACTACCTATTCCAAGTACATGGCTGTTAGAAAGCTCACATTCAACTGTGGTACCCTCGTTGTCACTCCGAAGACCTTAAGAAACCTTGCAAAACAACAGGTGGACTACATTTTAGGGGTAAACCCTCTGAAGATGTCCTACATGGTAGGGTATGGACCCTACTATCCCAAGAGAATTCACCACAGAGGATCCTCGTTGCCTTCATTGGCAAGCCATCCTCAGACCATAGGTTGTGATGGTGGTTTCCAACCATTCTTTTATTCGTTAAACCCAAACCCTAACATCTTAGTTGGAGCCGTTGTCGGAGGTCCAAACCAGAACGATGGGTTCCCAGATGACCGCAGTGATTACAATCACTCTGAGCCTGCAACGTACATAAATGGCGCTATTGTAGGACCCTTGGCATACTTTGCAGGGAGCTTATAA

Protein Analysis

496

Amino Acids

54.56

Weight (kDa)

9.15

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 35 - 486 5.7e-155 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1486
Acc65I GGTACC 1 cut(s) 1112
AccB1I GGYRCC 1 cut(s) 1112
AccIII TCCGGA 1 cut(s) 591
AciI CCGC 4 cut(s) 165, 561, 572, 1402
AclWI GGATC 5 cut(s) 443, 596, 700, 1245, 1258
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 2 cut(s) 854, 1236
AcuI CTGAAG 1 cut(s) 1211
AfaI GTAC 6 cut(s) 512, 665, 982, 1079, 1114, 1436
AfiI CCNNNNNNNGG 1 cut(s) 538
AflII CTTAAG 1 cut(s) 1138
AgsI TTSAA 8 cut(s) 199, 280, 329, 628, 828, 898, 978, 1105
AjnI CCWGG 2 cut(s) 114, 1018
AluBI AGCT 8 cut(s) 86, 742, 755, 818, 843, 1015, 1096, 1482
AluI AGCT 8 cut(s) 86, 742, 755, 818, 843, 1015, 1096, 1482
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 4 cut(s) 151, 258, 523, 548
AlwI GGATC 5 cut(s) 443, 596, 700, 1245, 1258
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 366, 532
Aor13HI TCCGGA 1 cut(s) 591
AoxI GGCC 5 cut(s) 111, 346, 384, 479, 633
ApeKI GCWGC 7 cut(s) 173, 371, 563, 575, 742, 755, 1015
ApoI RAATTY 2 cut(s) 854, 1236
ArsI GACNNNNNNTTYG 4 cut(s) 839, 871, 896, 928
Asp718I GGTACC 1 cut(s) 1112
AspLEI GCGC 2 cut(s) 342, 1448
AspS9I GGNCC 6 cut(s) 112, 384, 460, 1217, 1370, 1457
AsuC2I CCSGG 5 cut(s) 367, 368, 423, 533, 534
AsuHPI GGTGA 5 cut(s) 289, 458, 513, 960, 1232
AvaI CYCGRG 2 cut(s) 366, 532
AvaII GGWCC 4 cut(s) 460, 1217, 1370, 1457
BaeGI GKGCMC 1 cut(s) 892
BaeI ACNNNNGTAYC 4 cut(s) 1096, 1104, 1129, 1137
BamHI GGATCC 1 cut(s) 1250
BanI GGYRCC 1 cut(s) 1112
BanII GRGCYC 2 cut(s) 539, 1005
BarI GAAGNNNNNNTAC 2 cut(s) 1184, 1216
BbsI GAAGAC 2 cut(s) 492, 1139
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 7 cut(s) 160, 383, 550, 562, 729, 742, 1002
BccI CCATC 4 cut(s) 209, 1284, 1292, 1379
BceAI ACGGC 2 cut(s) 648, 1343
BciT130I CCWGG 2 cut(s) 116, 1020
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 5 cut(s) 367, 368, 423, 533, 534
BcoDI GTCTC 4 cut(s) 151, 258, 523, 548
BfaI CTAG 2 cut(s) 411, 819
BfmI CTRYAG 2 cut(s) 87, 696
BfoI RGCGCY 1 cut(s) 1449
BfrI CTTAAG 1 cut(s) 1138
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 9 cut(s) 174, 372, 561, 564, 573, 576, 743, 756, 1016
BlsI GCNGC 9 cut(s) 175, 373, 562, 565, 574, 577, 744, 757, 1017
BmcAI AGTACT 1 cut(s) 982
Bme1390I CCNGG 7 cut(s) 116, 367, 368, 423, 533, 534, 1020
Bme18I GGWCC 4 cut(s) 460, 1217, 1370, 1457
BmeT110I CYCGRG 2 cut(s) 366, 532
BmgT120I GGNCC 6 cut(s) 112, 384, 460, 1217, 1370, 1457
BmiI GGNNCC 6 cut(s) 1114, 1219, 1252, 1357, 1391, 1459
BmrFI CCNGG 7 cut(s) 116, 367, 368, 423, 533, 534, 1020
BmsI GCATC 1 cut(s) 636
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 2 cut(s) 492, 1139
BpmI CTGGAG 4 cut(s) 264, 282, 335, 570
BpuMI CCSGG 5 cut(s) 367, 368, 423, 533, 534
BsaBI GATNNNNATC 1 cut(s) 1413
BsaJI CCNNGG 7 cut(s) 114, 366, 387, 532, 667, 810, 1461
BsaWI WCCGGW 1 cut(s) 591
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 1347, 1377
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 247, 352
Bse8I GATNNNNATC 1 cut(s) 1413
BseAI TCCGGA 1 cut(s) 591
BseBI CCWGG 2 cut(s) 116, 1020
BseDI CCNNGG 7 cut(s) 114, 366, 387, 532, 667, 810, 1461
BseGI GGATG 2 cut(s) 742, 1276
BseJI GATNNNNATC 1 cut(s) 1413
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMII CTCAG 3 cut(s) 528, 1295, 1413
BseNI ACTGG 2 cut(s) 247, 352
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 7 cut(s) 160, 383, 550, 562, 729, 742, 1002
BseYI CCCAGC 1 cut(s) 843
BshFI GGCC 5 cut(s) 113, 348, 386, 481, 635
BshNI GGYRCC 1 cut(s) 1112
BsiHKAI GWGCWC 1 cut(s) 325
BsiHKCI CYCGRG 2 cut(s) 366, 532
BsiSI CCGG 6 cut(s) 168, 367, 423, 533, 592, 952
BslFI GGGAC 2 cut(s) 156, 860
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 4 cut(s) 151, 258, 523, 548
BsmFI GGGAC 2 cut(s) 156, 860
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 5 cut(s) 113, 348, 386, 481, 635
BsoBI CYCGRG 2 cut(s) 366, 532
Bsp1286I GDGCHC 4 cut(s) 325, 539, 892, 1005
Bsp13I TCCGGA 1 cut(s) 591
Bsp143I GATC 4 cut(s) 448, 601, 692, 1250
BspACI CCGC 4 cut(s) 165, 561, 572, 1402
BspANI GGCC 5 cut(s) 113, 348, 386, 481, 635
BspCNI CTCAG 3 cut(s) 527, 1294, 1414
BspEI TCCGGA 1 cut(s) 591
BspLI GGNNCC 6 cut(s) 1114, 1219, 1252, 1357, 1391, 1459
BspMAI CTGCAG 1 cut(s) 700
BspPI GGATC 5 cut(s) 443, 596, 700, 1245, 1258
BspT107I GGYRCC 1 cut(s) 1112
BspTI CTTAAG 1 cut(s) 1138
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 247, 352
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 7 cut(s) 114, 366, 387, 532, 667, 810, 1461
BssMI GATC 4 cut(s) 448, 601, 692, 1250
BssT1I CCWWGG 2 cut(s) 810, 1461
Bst2UI CCWGG 2 cut(s) 116, 1020
Bst4CI ACNGT 5 cut(s) 381, 508, 680, 894, 1109
Bst6I CTCTTC 3 cut(s) 32, 137, 979
BstAFI CTTAAG 1 cut(s) 1138
BstAPI GCANNNNNTGC 1 cut(s) 1472
BstC8I GCNNGC 3 cut(s) 338, 1273, 1428
BstDEI CTNAG 5 cut(s) 500, 514, 1281, 1348, 1422
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 742, 1276
BstH2I RGCGCY 1 cut(s) 1449
BstHHI GCGC 2 cut(s) 342, 1448
BstKTI GATC 4 cut(s) 451, 604, 695, 1253
BstMAI GTCTC 4 cut(s) 151, 258, 523, 548
BstMBI GATC 4 cut(s) 448, 601, 692, 1250
BstMWI GCNNNNNNNGC 6 cut(s) 173, 371, 534, 569, 572, 1472
BstNI CCWGG 2 cut(s) 116, 1020
BstPAI GACNNNNGTC 1 cut(s) 148
BstSCI CCNGG 7 cut(s) 114, 365, 366, 421, 531, 532, 1018
BstSFI CTRYAG 2 cut(s) 87, 696
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 7 cut(s) 160, 383, 550, 562, 729, 742, 1002
BstV2I GAAGAC 2 cut(s) 492, 1139
BstX2I RGATCY 2 cut(s) 692, 1250
BstXI CCANNNNNNTGG 2 cut(s) 1081, 1385
BstYI RGATCY 2 cut(s) 692, 1250
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 5 cut(s) 113, 348, 386, 481, 635
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 742, 1276
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 3 cut(s) 685, 890, 1411
Cac8I GCNNGC 3 cut(s) 338, 1273, 1428
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 2 cut(s) 342, 1448
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 6 cut(s) 112, 384, 460, 1217, 1370, 1457
Cfr9I CCCGGG 2 cut(s) 366, 532
CseI GACGC 1 cut(s) 266
Csp6I GTAC 6 cut(s) 511, 664, 981, 1078, 1113, 1435
CviAII CATG 6 cut(s) 189, 315, 490, 759, 1081, 1204
CviQI GTAC 6 cut(s) 511, 664, 981, 1078, 1113, 1435
DdeI CTNAG 5 cut(s) 500, 514, 1281, 1348, 1422
DpnI GATC 4 cut(s) 450, 603, 694, 1252
DpnII GATC 4 cut(s) 448, 601, 692, 1250
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 3 cut(s) 32, 137, 979
EarI CTCTTC 3 cut(s) 32, 137, 979
Eco130I CCWWGG 2 cut(s) 810, 1461
Eco24I GRGCYC 2 cut(s) 539, 1005
Eco47I GGWCC 4 cut(s) 460, 1217, 1370, 1457
Eco57I CTGAAG 1 cut(s) 1211
Eco88I CYCGRG 2 cut(s) 366, 532
EcoO109I RGGNCCY 2 cut(s) 112, 1457
EcoRI GAATTC 1 cut(s) 1236
EcoRII CCWGG 2 cut(s) 114, 1018
EcoT14I CCWWGG 2 cut(s) 810, 1461
EcoT38I GRGCYC 2 cut(s) 539, 1005
ErhI CCWWGG 2 cut(s) 810, 1461
FaeI CATG 6 cut(s) 192, 318, 493, 762, 1084, 1207
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 2 cut(s) 156, 860
FatI CATG 6 cut(s) 188, 314, 489, 758, 1080, 1203
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 9 cut(s) 174, 372, 561, 564, 573, 576, 743, 756, 1016
FokI GGATG 2 cut(s) 749, 1263
FriOI GRGCYC 2 cut(s) 539, 1005
Fsp4HI GCNGC 9 cut(s) 174, 372, 561, 564, 573, 576, 743, 756, 1016
FspBI CTAG 2 cut(s) 411, 819
GlaI GCGC 2 cut(s) 341, 1447
GluI GCNGC 9 cut(s) 174, 372, 561, 564, 573, 576, 743, 756, 1016
GsaI CCCAGC 1 cut(s) 847
GsuI CTGGAG 4 cut(s) 264, 282, 335, 570
HaeII RGCGCY 1 cut(s) 1449
HaeIII GGCC 5 cut(s) 113, 348, 386, 481, 635
HapII CCGG 6 cut(s) 168, 367, 423, 533, 592, 952
HgaI GACGC 1 cut(s) 266
HhaI GCGC 2 cut(s) 342, 1448
Hin1II CATG 6 cut(s) 192, 318, 493, 762, 1084, 1207
Hin6I GCGC 2 cut(s) 340, 1446
HinP1I GCGC 2 cut(s) 340, 1446
HinfI GANTC 3 cut(s) 159, 683, 824
HpaII CCGG 6 cut(s) 168, 367, 423, 533, 592, 952
HphI GGTGA 5 cut(s) 289, 458, 513, 960, 1232
Hpy166II GTNNAC 6 cut(s) 235, 428, 460, 511, 1165, 1183
Hpy188I TCNGA 8 cut(s) 214, 541, 606, 1131, 1191, 1284, 1367, 1423
Hpy188III TCNNGA 2 cut(s) 592, 726
Hpy8I GTNNAC 6 cut(s) 235, 428, 460, 511, 1165, 1183
HpyAV CCTTC 2 cut(s) 589, 1272
HpyCH4III ACNGT 5 cut(s) 381, 508, 680, 894, 1109
HpyCH4IV ACGT 2 cut(s) 543, 1433
HpyCH4V TGCA 8 cut(s) 566, 649, 656, 698, 758, 1151, 1430, 1475
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 371, 534, 569, 572, 1472
HpyF3I CTNAG 5 cut(s) 500, 514, 1281, 1348, 1422
HpySE526I ACGT 2 cut(s) 543, 1433
Hsp92II CATG 6 cut(s) 192, 318, 493, 762, 1084, 1207
HspAI GCGC 2 cut(s) 340, 1446
Kpn2I TCCGGA 1 cut(s) 591
KpnI GGTACC 1 cut(s) 1116
Kzo9I GATC 4 cut(s) 448, 601, 692, 1250
LmnI GCTCC 5 cut(s) 320, 752, 815, 1355, 1479
Lsp1109I GCAGC 7 cut(s) 160, 383, 550, 562, 729, 742, 1002
LweI GCATC 1 cut(s) 636
MaeI CTAG 2 cut(s) 411, 819
MaeII ACGT 2 cut(s) 543, 1433
MaeIII GTNAC 4 cut(s) 354, 1025, 1039, 1123
MalI GATC 4 cut(s) 450, 603, 694, 1252
MboI GATC 4 cut(s) 448, 601, 692, 1250
MboII GAAGA 7 cut(s) 19, 124, 389, 497, 966, 1144, 1204
MflI RGATCY 2 cut(s) 692, 1250
MhlI GDGCHC 4 cut(s) 325, 539, 892, 1005
MluCI AATT 7 cut(s) 100, 199, 657, 796, 854, 941, 1236
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 3 cut(s) 1333, 1333, 1345
MroI TCCGGA 1 cut(s) 591
MseI TTAA 3 cut(s) 239, 1139, 1328
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 563, 843
MspCI CTTAAG 1 cut(s) 1138
MspI CCGG 6 cut(s) 168, 367, 423, 533, 592, 952
MspR9I CCNGG 7 cut(s) 116, 367, 368, 423, 533, 534, 1020
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 116, 1020
MwoI GCNNNNNNNGC 6 cut(s) 173, 371, 534, 569, 572, 1472
NciI CCSGG 5 cut(s) 367, 368, 423, 533, 534
NdeII GATC 4 cut(s) 448, 601, 692, 1250
NlaIII CATG 6 cut(s) 192, 318, 493, 762, 1084, 1207
NlaIV GGNNCC 6 cut(s) 1114, 1219, 1252, 1357, 1391, 1459
NmuCI GTSAC 2 cut(s) 1039, 1123
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 683, 824
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 9 cut(s) 175, 373, 562, 565, 574, 577, 744, 757, 1017
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
PpuMI RGGWCCY 1 cut(s) 1457
PshAI GACNNNNGTC 1 cut(s) 148
PsiI TTATAA 1 cut(s) 1486
Psp5II RGGWCCY 1 cut(s) 1457
Psp6I CCWGG 2 cut(s) 114, 1018
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 2 cut(s) 114, 1018
PspN4I GGNNCC 6 cut(s) 1114, 1219, 1252, 1357, 1391, 1459
PspPI GGNCC 6 cut(s) 112, 384, 460, 1217, 1370, 1457
PspPPI RGGWCCY 1 cut(s) 1457
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 2 cut(s) 692, 1250
PsyI GACNNNGTC 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 843
RsaI GTAC 6 cut(s) 512, 665, 982, 1079, 1114, 1436
RsaNI GTAC 6 cut(s) 511, 664, 981, 1078, 1113, 1435
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 3 cut(s) 239, 1139, 1328
SatI GCNGC 9 cut(s) 174, 372, 561, 564, 573, 576, 743, 756, 1016
Sau3AI GATC 4 cut(s) 448, 601, 692, 1250
Sau96I GGNCC 6 cut(s) 112, 384, 460, 1217, 1370, 1457
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 7 cut(s) 116, 367, 368, 423, 533, 534, 1020
SduI GDGCHC 4 cut(s) 325, 539, 892, 1005
SfaNI GCATC 1 cut(s) 636
SfcI CTRYAG 2 cut(s) 87, 696
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 4 cut(s) 460, 1217, 1370, 1457
SmaI CCCGGG 2 cut(s) 368, 534
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 1138
SmoI CTYRAG 1 cut(s) 1138
SrfI GCCCGGGC 1 cut(s) 368
Sse9I AATT 7 cut(s) 100, 199, 657, 796, 854, 941, 1236
SsiI CCGC 4 cut(s) 165, 561, 572, 1402
SspI AATATT 1 cut(s) 48
SspMI CTAG 2 cut(s) 411, 819
StyD4I CCNGG 7 cut(s) 114, 365, 366, 421, 531, 532, 1018
StyI CCWWGG 2 cut(s) 810, 1461
TaaI ACNGT 5 cut(s) 381, 508, 680, 894, 1109
TaiI ACGT 2 cut(s) 546, 1436
TasI AATT 7 cut(s) 100, 199, 657, 796, 854, 941, 1236
TatI WGTACW 3 cut(s) 510, 980, 1077
TauI GCSGC 2 cut(s) 563, 575
TfiI GAWTC 2 cut(s) 683, 824
Tru1I TTAA 3 cut(s) 239, 1139, 1328
Tru9I TTAA 3 cut(s) 239, 1139, 1328
TscAI CASTG 3 cut(s) 685, 897, 1411
TseFI GTSAC 2 cut(s) 1039, 1123
TseI GCWGC 7 cut(s) 173, 371, 563, 575, 742, 755, 1015
Tsp45I GTSAC 2 cut(s) 1039, 1123
TspDTI ATGAA 4 cut(s) 19, 795, 818, 1254
TspGWI ACGGA 1 cut(s) 404
TspMI CCCGGG 2 cut(s) 366, 532
TspRI CASTG 3 cut(s) 685, 897, 1411
Tth111I GACNNNGTC 1 cut(s) 269
Vha464I CTTAAG 1 cut(s) 1138
VpaK11BI GGWCC 4 cut(s) 460, 1217, 1370, 1457
XapI RAATTY 2 cut(s) 854, 1236
XmaI CCCGGG 2 cut(s) 366, 532
XspI CTAG 2 cut(s) 411, 819
ZrmI AGTACT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.