RchiOBHm_Chr6g0251051

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006215
Physical Location & Seq
Reverse (-)
6362908 .. 6366150
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22506

Sequence Viewer

Length: 1464 bp
ATGTCTTCGTTGTTCCTCATCTCTGTGTTGATATCATTGGGCACTAGTATTGGTATAGTCCAAGCAGGCAACCCAAATTACAGAGATGCCCTTGCAAAGTCCATCCTCTTCTTCCAGGGACAGAGGTCAGGCAAGCTCCCCTCCGGCGCTGCCCAGCAAATTACCTGGAGGTCCAATTCTGGACTTTCCGATGGTCAACTCGCAAAAGTGGACTTAACTGGAGGATACTATGATGGTGGAGACAATGTCAAATTCAACTTCCCAATGGCTTTCACTGCGACAATGCTATCATGGGGATCACTTGAATATAGCAAAAAAATGGAGCCCCAACTAACGAATACCCGAGCCGCAATCCGATGGTCCGCAAACTACCTTCTAAAGTGTGCTCGAGCCACGCCCGATAGGCTCTACGTTGGGATCGGGGACCCTAATGTAGACCACGAGTGTTGGGAAAGGCCCGAAGACATGGACACGGCTCGAACCGTGTACTGGGTCTCACCTAGCAATCCTGGTTCGGATGTTGCTGGAGAGACGGCGGCCGCATTGGCTGCTGCTTCTATGGTTTTCCGAAGGGTTGACCTCAAGTATTCAAAATTGTTATTGAGAACAGCCAAGAAAGTATTTCAGTTTGCAATTCAGTACAAAGGTGCCTACAGTGATTCACTTGGTTCTGCTGTTTGCCCATTTTATTGCTCATATTCAGGATACATGGATGAGCTACTTTGGGGTGCTGCATGGCTTCTTAGAGCAACAAATGATGTCTTTTATTTCAATTTCTTGAAATCCTTGGGAGCTGGTGATTCACCTGATATCTTCAGCTGGGACAACAAATTTGCTGGTGCTTATGTTCTGCTATCAAGGAGGGCAGTGTTGAACAATGACAAGAATTTTGACTCATATAAACAACAAGCTGAGCAATTTATGTGCCGGATTTTGCCCAACTCACCCTCTTCAAGCACACAATATACAAAAGGAGGGCTCATATTCAAGCTGCCTGAAAGTAATCTCCAATATGTCACCTCAATAACATTCTTGCTTACTACCTATTCCAAGTACATGTCTGCCACAAAGCACAGGTTTAACTGTGGCAATCTTGTTGTCAATTCAAGCACTTTAAGAAACATTGCCAAGAGACAGGTGGACTACATATTAGGGGTAAACCCACTGAACATGTCCTACATGGTAGGTTATGGACCTCGCTATCCTAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCATTGGCAAGTCATCCAAAAACCATAGGTTGTAAAGCTGGTTTCCAACCATTCTTCTATTCATCAAATCCAAATCCTAACCTCCTAGTTGGAGCTGTTGTTGGAGGTCCAAATCAGAATGATGGGTTTCCAGATGATCGCACCGATTATAGTCATTCAGAGCCTGCAACATACATAAATGGTGCTATTGTCGGACCATTAGCATACTTTGCAAATCTCAATTGA

Protein Analysis

487

Amino Acids

53.77

Weight (kDa)

9.18

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 27 - 478 9.8e-154 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 435
AciI CCGC 4 cut(s) 348, 363, 536, 540
AclWI GGATC 3 cut(s) 304, 425, 1234
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 4 cut(s) 251, 830, 886, 1212
AcuI CTGAAG 1 cut(s) 799
AfaI GTAC 3 cut(s) 488, 641, 1055
AfiI CCNNNNNNNGG 1 cut(s) 489
AflIII ACRYGT 2 cut(s) 1056, 1170
AgsI TTSAA 9 cut(s) 256, 305, 591, 772, 781, 874, 954, 988, 1107
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 3 cut(s) 114, 164, 508
AluBI AGCT 8 cut(s) 136, 718, 794, 819, 911, 991, 1277, 1334
AluI AGCT 8 cut(s) 136, 718, 794, 819, 911, 991, 1277, 1334
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 4 cut(s) 234, 499, 524, 1126
AlwI GGATC 3 cut(s) 304, 425, 1234
AlwNI CAGNNNCTG 1 cut(s) 1403
Ama87I CYCGRG 2 cut(s) 342, 387
AoxI GGCC 2 cut(s) 455, 537
ApeKI GCWGC 5 cut(s) 149, 548, 551, 731, 991
ApoI RAATTY 4 cut(s) 251, 830, 886, 1212
ArsI GACNNNNNNTTYG 4 cut(s) 815, 847, 872, 904
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 7 cut(s) 171, 360, 424, 456, 1193, 1346, 1433
AsuHPI GGTGA 6 cut(s) 489, 795, 809, 936, 1009, 1208
AvaI CYCGRG 2 cut(s) 342, 387
AvaII GGWCC 6 cut(s) 171, 360, 424, 1193, 1346, 1433
BaeGI GKGCMC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 2 cut(s) 327, 981
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 5 cut(s) 136, 535, 538, 718, 978
BccI CCATC 5 cut(s) 110, 185, 227, 351, 1355
BceAI ACGGC 2 cut(s) 489, 549
BciT130I CCWGG 3 cut(s) 116, 166, 510
BciVI GTATCC 2 cut(s) 218, 698
BcoDI GTCTC 4 cut(s) 234, 499, 524, 1126
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 3 cut(s) 45, 501, 1325
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 2 cut(s) 218, 698
BglI GCCNNNNNGGC 2 cut(s) 403, 545
BisI GCNGC 8 cut(s) 150, 348, 537, 540, 549, 552, 732, 992
BlpI GCTNAGC 1 cut(s) 912
BlsI GCNGC 8 cut(s) 151, 349, 538, 541, 550, 553, 733, 993
Bme1390I CCNGG 3 cut(s) 116, 166, 510
Bme18I GGWCC 6 cut(s) 171, 360, 424, 1193, 1346, 1433
BmeT110I CYCGRG 2 cut(s) 342, 387
BmgT120I GGNCC 7 cut(s) 171, 360, 424, 456, 1193, 1346, 1433
BmiI GGNNCC 4 cut(s) 324, 425, 426, 649
BmrFI CCNGG 3 cut(s) 116, 166, 510
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 499
BoxI GACNNNNGTC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 3 cut(s) 187, 240, 546
Bpu1102I GCTNAGC 1 cut(s) 912
BpuEI CTTGAG 1 cut(s) 566
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 115, 786
BsaXI ACNNNNNCTCC 4 cut(s) 231, 261, 1323, 1353
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 2 cut(s) 223, 494
Bse3DI GCAATG 2 cut(s) 1122, 1232
BseBI CCWGG 3 cut(s) 116, 166, 510
BseDI CCNNGG 2 cut(s) 115, 786
BseGI GGATG 4 cut(s) 102, 523, 718, 1252
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 2 cut(s) 1122, 1232
BseMII CTCAG 1 cut(s) 903
BseNI ACTGG 2 cut(s) 223, 494
BseSI GKGCMC 1 cut(s) 44
BseX3I CGGCCG 1 cut(s) 537
BseXI GCAGC 5 cut(s) 136, 535, 538, 718, 978
BseYI CCCAGC 2 cut(s) 153, 819
Bsh1285I CGRYCG 1 cut(s) 540
BshFI GGCC 2 cut(s) 457, 539
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 2 cut(s) 342, 387
BsiSI CCGG 2 cut(s) 144, 928
BslFI GGGAC 3 cut(s) 132, 437, 836
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 4 cut(s) 234, 499, 524, 1126
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 3 cut(s) 132, 437, 836
BsnI GGCC 2 cut(s) 457, 539
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 2 cut(s) 342, 387
Bsp1286I GDGCHC 4 cut(s) 44, 327, 388, 981
Bsp143I GATC 4 cut(s) 296, 417, 1226, 1375
Bsp1720I GCTNAGC 1 cut(s) 912
BspACI CCGC 4 cut(s) 348, 363, 536, 540
BspANI GGCC 2 cut(s) 457, 539
BspCNI CTCAG 1 cut(s) 904
BspLI GGNNCC 4 cut(s) 324, 425, 426, 649
BspPI GGATC 3 cut(s) 304, 425, 1234
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 2 cut(s) 1122, 1232
BsrI ACTGG 2 cut(s) 223, 494
BssECI CCNNGG 2 cut(s) 115, 786
BssMI GATC 4 cut(s) 296, 417, 1226, 1375
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 786
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 3 cut(s) 116, 166, 510
Bst4CI ACNGT 3 cut(s) 484, 656, 1085
Bst6I CTCTTC 2 cut(s) 113, 955
BstAPI GCANNNNNTGC 2 cut(s) 548, 1448
BstC8I GCNNGC 3 cut(s) 67, 134, 1404
BstDEI CTNAG 3 cut(s) 743, 912, 1206
BstF5I GGATG 4 cut(s) 102, 523, 718, 1252
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 4 cut(s) 299, 420, 1229, 1378
BstMAI GTCTC 4 cut(s) 234, 499, 524, 1126
BstMBI GATC 4 cut(s) 296, 417, 1226, 1375
BstMCI CGRYCG 1 cut(s) 540
BstMWI GCNNNNNNNGC 5 cut(s) 275, 403, 545, 548, 1448
BstNI CCWGG 3 cut(s) 116, 166, 510
BstNSI RCATGY 2 cut(s) 1060, 1174
BstPAI GACNNNNGTC 1 cut(s) 124
BstSCI CCNGG 3 cut(s) 114, 164, 508
BstSFI CTRYAG 1 cut(s) 652
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 5 cut(s) 136, 535, 538, 718, 978
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 1226
BstYI RGATCY 1 cut(s) 1226
BstZI CGGCCG 1 cut(s) 537
BsuI GTATCC 2 cut(s) 218, 698
BsuRI GGCC 2 cut(s) 457, 539
BtsCI GGATG 4 cut(s) 102, 523, 718, 1252
BtsI GCAGTG 2 cut(s) 273, 873
BtsIMutI CAGTG 4 cut(s) 273, 661, 873, 1163
Cac8I GCNNGC 3 cut(s) 67, 134, 1404
CaiI CAGNNNCTG 1 cut(s) 1403
CciNI GCGGCCGC 1 cut(s) 537
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 7 cut(s) 171, 360, 424, 456, 1193, 1346, 1433
Csp6I GTAC 3 cut(s) 487, 640, 1054
CspCI CAANNNNNGTGG 2 cut(s) 428, 463
CviAII CATG 7 cut(s) 291, 466, 709, 735, 1057, 1171, 1180
CviQI GTAC 3 cut(s) 487, 640, 1054
DdeI CTNAG 3 cut(s) 743, 912, 1206
DpnI GATC 4 cut(s) 298, 419, 1228, 1377
DpnII GATC 4 cut(s) 296, 417, 1226, 1375
EaeI YGGCCR 1 cut(s) 537
EagI CGGCCG 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 113, 955
EarI CTCTTC 2 cut(s) 113, 955
EclXI CGGCCG 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 786
Eco24I GRGCYC 2 cut(s) 327, 981
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 2 cut(s) 33, 811
Eco47I GGWCC 6 cut(s) 171, 360, 424, 1193, 1346, 1433
Eco52I CGGCCG 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 799
Eco88I CYCGRG 2 cut(s) 342, 387
EcoO109I RGGNCCY 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 1212
EcoRII CCWGG 3 cut(s) 114, 164, 508
EcoRV GATATC 2 cut(s) 33, 811
EcoT14I CCWWGG 1 cut(s) 786
EcoT38I GRGCYC 2 cut(s) 327, 981
ErhI CCWWGG 1 cut(s) 786
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 7 cut(s) 294, 469, 712, 738, 1060, 1174, 1183
FaqI GGGAC 3 cut(s) 132, 437, 836
FatI CATG 7 cut(s) 290, 465, 708, 734, 1056, 1170, 1179
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 8 cut(s) 150, 348, 537, 540, 549, 552, 732, 992
FokI GGATG 4 cut(s) 89, 530, 725, 1239
FriOI GRGCYC 2 cut(s) 327, 981
Fsp4HI GCNGC 8 cut(s) 150, 348, 537, 540, 549, 552, 732, 992
FspBI CTAG 3 cut(s) 45, 501, 1325
GlaI GCGC 1 cut(s) 148
GluI GCNGC 8 cut(s) 150, 348, 537, 540, 549, 552, 732, 992
GsaI CCCAGC 2 cut(s) 157, 823
GsuI CTGGAG 3 cut(s) 187, 240, 546
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 457, 539
HapII CCGG 2 cut(s) 144, 928
HhaI GCGC 1 cut(s) 149
Hin1II CATG 7 cut(s) 294, 469, 712, 738, 1060, 1174, 1183
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 2 cut(s) 197, 577
HindII GTYRAC 2 cut(s) 197, 577
HinfI GANTC 3 cut(s) 659, 800, 893
HpaII CCGG 2 cut(s) 144, 928
HphI GGTGA 6 cut(s) 489, 795, 809, 936, 1009, 1208
Hpy166II GTNNAC 7 cut(s) 197, 211, 436, 487, 577, 1141, 1159
Hpy188I TCNGA 7 cut(s) 190, 356, 517, 569, 1356, 1399, 1433
Hpy188III TCNNGA 4 cut(s) 180, 702, 778, 1370
Hpy8I GTNNAC 7 cut(s) 197, 211, 436, 487, 577, 1141, 1159
HpyAV CCTTC 2 cut(s) 383, 564
HpyCH4III ACNGT 3 cut(s) 484, 656, 1085
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 95, 632, 734, 1406, 1451
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 403, 545, 548, 1448
HpyF3I CTNAG 3 cut(s) 743, 912, 1206
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 7 cut(s) 294, 469, 712, 738, 1060, 1174, 1183
HspAI GCGC 1 cut(s) 147
KflI GGGWCCC 1 cut(s) 424
Kzo9I GATC 4 cut(s) 296, 417, 1226, 1375
LmnI GCTCC 4 cut(s) 141, 322, 791, 1331
Lsp1109I GCAGC 5 cut(s) 136, 535, 538, 718, 978
LweI GCATC 1 cut(s) 76
MaeI CTAG 3 cut(s) 45, 501, 1325
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 1 cut(s) 1015
MalI GATC 4 cut(s) 298, 419, 1228, 1377
MboI GATC 4 cut(s) 296, 417, 1226, 1375
MboII GAAGA 7 cut(s) 100, 103, 473, 805, 942, 1221, 1285
MfeI CAATTG 1 cut(s) 1459
MflI RGATCY 1 cut(s) 1226
MhlI GDGCHC 4 cut(s) 44, 327, 388, 981
MlyI GAGTC 1 cut(s) 887
MmeI TCCRAC 4 cut(s) 1309, 1309, 1321, 1411
MseI TTAA 3 cut(s) 215, 1080, 1115
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 819
MspI CCGG 2 cut(s) 144, 928
MspR9I CCNGG 3 cut(s) 116, 166, 510
MunI CAATTG 1 cut(s) 1459
MvaI CCWGG 3 cut(s) 116, 166, 510
MwoI GCNNNNNNNGC 5 cut(s) 275, 403, 545, 548, 1448
NdeII GATC 4 cut(s) 296, 417, 1226, 1375
NlaIII CATG 7 cut(s) 294, 469, 712, 738, 1060, 1174, 1183
NlaIV GGNNCC 4 cut(s) 324, 425, 426, 649
NmuCI GTSAC 1 cut(s) 1015
NotI GCGGCCGC 1 cut(s) 537
NspI RCATGY 2 cut(s) 1060, 1174
PaeR7I CTCGAG 1 cut(s) 387
PciI ACATGT 2 cut(s) 1056, 1170
PfeI GAWTC 2 cut(s) 659, 800
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 8 cut(s) 151, 349, 538, 541, 550, 553, 733, 993
PleI GAGTC 1 cut(s) 887
PpsI GAGTC 1 cut(s) 887
PpuMI RGGWCCY 1 cut(s) 424
PscI ACATGT 2 cut(s) 1056, 1170
PshAI GACNNNNGTC 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 3 cut(s) 114, 164, 508
PspFI CCCAGC 2 cut(s) 153, 819
PspGI CCWGG 3 cut(s) 114, 164, 508
PspN4I GGNNCC 4 cut(s) 324, 425, 426, 649
PspPI GGNCC 7 cut(s) 171, 360, 424, 456, 1193, 1346, 1433
PspPPI RGGWCCY 1 cut(s) 424
PspXI VCTCGAGB 1 cut(s) 387
PsrI GAACNNNNNNTAC 2 cut(s) 1160, 1192
PstNI CAGNNNCTG 1 cut(s) 1403
PsuI RGATCY 1 cut(s) 1226
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 819
RsaI GTAC 3 cut(s) 488, 641, 1055
RsaNI GTAC 3 cut(s) 487, 640, 1054
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 3 cut(s) 215, 1080, 1115
SatI GCNGC 8 cut(s) 150, 348, 537, 540, 549, 552, 732, 992
Sau3AI GATC 4 cut(s) 296, 417, 1226, 1375
Sau96I GGNCC 7 cut(s) 171, 360, 424, 456, 1193, 1346, 1433
SchI GAGTC 1 cut(s) 887
ScrFI CCNGG 3 cut(s) 116, 166, 510
SduI GDGCHC 4 cut(s) 44, 327, 388, 981
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 6 cut(s) 171, 360, 424, 1193, 1346, 1433
SlaI CTCGAG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 387, 581
SmoI CTYRAG 2 cut(s) 387, 581
SpeI ACTAGT 1 cut(s) 44
SsiI CCGC 4 cut(s) 348, 363, 536, 540
SspMI CTAG 3 cut(s) 45, 501, 1325
StyD4I CCNGG 3 cut(s) 114, 164, 508
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 3 cut(s) 484, 656, 1085
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 388, 478
TatI WGTACW 3 cut(s) 486, 639, 1053
TauI GCSGC 3 cut(s) 350, 539, 542
TfiI GAWTC 2 cut(s) 659, 800
Tru1I TTAA 3 cut(s) 215, 1080, 1115
Tru9I TTAA 3 cut(s) 215, 1080, 1115
TscAI CASTG 4 cut(s) 280, 661, 873, 1170
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 5 cut(s) 149, 548, 551, 731, 991
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 2 cut(s) 1221, 1290
TspRI CASTG 4 cut(s) 280, 661, 873, 1170
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 6 cut(s) 171, 360, 424, 1193, 1346, 1433
XapI RAATTY 4 cut(s) 251, 830, 886, 1212
XceI RCATGY 2 cut(s) 1060, 1174
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XhoI CTCGAG 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 3 cut(s) 45, 501, 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.