Rroxscaffold_7G00217080

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006215
Physical Location & Seq
Forward (+)
67632002 .. 67635454
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217080.1

Sequence Viewer

Length: 1470 bp
ATGGCAATGTCTTCGTTGTTCCTCATCTCTGTGCTGATATCATTGGGCATTAGTATTGGTTTAGTCCAAGCAGGCAACCCAAATTACAGAGATGCCCTTGCAAAGTCCATCCTCTTCTTCCAGGGACAGAGGTCAGGCAAGCTACCCTCCGGCGCTGCCCAGCAAATTACCTGGAGGTCCAATTCTGGACTTTCCGATGGTCAACTCGCAAAAGTGGACTTAACTGGAGGATACTATGACGGTGGAGACAATGTCAAATTCAACTTCCCAATGGCTTTCACTGCCACAATGCTATCATGGGGATCACTTGAATATAGCGAAAAAATGGGGCCCCAACTAACGAATACCCGGGCCGCAATCCGATGGTCCGCAAACTACCTTCTAAAGTGTGCTCGAGCCACGCCCGATAGGCTCTACGTTGGGATCGGGGACCCTAATGTAGACCACAAGTGTTGGGAAAGGCCCGAAGACATGGACACGGCTCGAACCGTGTACTGGGTCTCACCTAGCAATCCTGGTTCAGATGTTGCTGGAGAGACGGCGGCCGCATTGGCTGCTGGTTCTATGGTTTTCCGAAGTGTTGACCCTAAGTATTCAAAATTGTTATTAAGAACAGCCAAGAAAGTATTTCAGTTTGCAATTCAGTACAGAGGTGCCTACAGTGATTCACTTGGTTCTGCTGTTTGCCCATTTTATTGCTCATATTCAGGATACAAGGATGAGCTAGTTTGGGGTGCTGCATGGCTTCTTAGAGTAACAAATGATGTCTTTTATTTCAATTTCTTGAAATCCTTGGGAGCTGATGATTCACCAGATATATTCAGCTGGGACAACAAATTTGCTGGTGCTTATGTTCTGCTATCAAGGAGCGCAGTGTTGAACAATGACAAGAATTTTGACTCATATAAACAACAAGCTGAGCAATTTATGTGCCGGATTTTGCCCAACTCACCCTCTTCAAGCACACAATATACAAAAGGAGGGCTCATATTCAAGCTGCCTGAAAGTAACCTCCAATATGTCACCTCAATAACATTCTTGCTTACTACCTATTCCAAGTACATGTCTGCCACAAAGCACAGGTTTAACTGTGGCAATCTTGTTGTCACTTCAAGCACTTTAAGAAACATTGCCAAGAGACAGGTGGACTACATATTAGGGGTAAACCCACTGAACATGTCCTACATGGTAGGTTATGGACCTCGCTATCCTAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCGTTGGCAAGTCATCCAAAAACCATAGGTTGTAAAGCTGGTTTCCAACCATTCTTCTATTCATCAAATCCAAATCCTAACCTCCTAGTTGGAGCTGTTGTTGGAGGTCCAAATCAGAATGATGGGTTTTCAGATGATCGCACCGATTATGGTCATTCAGAGCCTGCAACATGCATAAATGGTGCTATAGTCGGACCATTAGCATACTTTGCAAATCTCAATTGA

Protein Analysis

489

Amino Acids

53.73

Weight (kDa)

9.09

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 29 - 480 6.5e-153 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 653
AccI GTMKAC 1 cut(s) 441
AciI CCGC 4 cut(s) 354, 369, 542, 546
AclWI GGATC 3 cut(s) 310, 431, 1240
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 4 cut(s) 257, 836, 892, 1218
AfaI GTAC 3 cut(s) 494, 647, 1061
AfiI CCNNNNNNNGG 1 cut(s) 495
AflIII ACRYGT 2 cut(s) 1062, 1176
AgsI TTSAA 9 cut(s) 262, 311, 597, 778, 787, 880, 960, 994, 1113
AjnI CCWGG 3 cut(s) 120, 170, 514
AluBI AGCT 8 cut(s) 142, 724, 800, 825, 917, 997, 1283, 1340
AluI AGCT 8 cut(s) 142, 724, 800, 825, 917, 997, 1283, 1340
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 4 cut(s) 240, 505, 530, 1132
AlwI GGATC 3 cut(s) 310, 431, 1240
AlwNI CAGNNNCTG 1 cut(s) 1409
Ama87I CYCGRG 2 cut(s) 348, 393
AoxI GGCC 4 cut(s) 329, 351, 461, 543
ApaI GGGCCC 1 cut(s) 333
ApeKI GCWGC 4 cut(s) 155, 554, 737, 997
ApoI RAATTY 4 cut(s) 257, 836, 892, 1218
ArsI GACNNNNNNTTYG 4 cut(s) 821, 853, 878, 910
AspLEI GCGC 2 cut(s) 155, 872
AsuC2I CCSGG 2 cut(s) 349, 350
AsuHPI GGTGA 5 cut(s) 495, 801, 942, 1015, 1214
AvaI CYCGRG 2 cut(s) 348, 393
AvaII GGWCC 6 cut(s) 177, 366, 430, 1199, 1352, 1439
BaeGI GKGCMC 1 cut(s) 333
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 2 cut(s) 333, 987
BbsI GAAGAC 2 cut(s) 3, 474
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 4 cut(s) 142, 541, 724, 984
BccI CCATC 4 cut(s) 116, 191, 357, 1361
BceAI ACGGC 2 cut(s) 495, 555
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 122, 172, 516
BciVI GTATCC 2 cut(s) 224, 704
BcnI CCSGG 2 cut(s) 349, 350
BcoDI GTCTC 4 cut(s) 240, 505, 530, 1132
BfaI CTAG 3 cut(s) 507, 725, 1331
BfmI CTRYAG 2 cut(s) 658, 1431
BfoI RGCGCY 1 cut(s) 156
BfuI GTATCC 2 cut(s) 224, 704
BglI GCCNNNNNGGC 2 cut(s) 409, 551
BisI GCNGC 7 cut(s) 156, 354, 543, 546, 555, 738, 998
BlpI GCTNAGC 1 cut(s) 918
BlsI GCNGC 7 cut(s) 157, 355, 544, 547, 556, 739, 999
Bme1390I CCNGG 5 cut(s) 122, 172, 349, 350, 516
Bme18I GGWCC 6 cut(s) 177, 366, 430, 1199, 1352, 1439
BmeT110I CYCGRG 2 cut(s) 348, 393
BmiI GGNNCC 6 cut(s) 330, 331, 332, 431, 432, 655
BmrFI CCNGG 5 cut(s) 122, 172, 349, 350, 516
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 82
BmuI ACTGGG 1 cut(s) 505
BoxI GACNNNNGTC 1 cut(s) 130
BpiI GAAGAC 2 cut(s) 3, 474
BpmI CTGGAG 3 cut(s) 193, 246, 552
Bpu1102I GCTNAGC 1 cut(s) 918
BpuMI CCSGG 2 cut(s) 349, 350
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 121, 348, 792
BsaXI ACNNNNNCTCC 4 cut(s) 237, 267, 1329, 1359
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 229, 500
Bse3DI GCAATG 3 cut(s) 12, 1128, 1238
BseBI CCWGG 3 cut(s) 122, 172, 516
BseDI CCNNGG 3 cut(s) 121, 348, 792
BseGI GGATG 3 cut(s) 108, 724, 1258
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMI GCAATG 3 cut(s) 12, 1128, 1238
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 2 cut(s) 229, 500
BseSI GKGCMC 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 543
BseXI GCAGC 4 cut(s) 142, 541, 724, 984
BseYI CCCAGC 2 cut(s) 159, 825
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 4 cut(s) 331, 353, 463, 545
BshNI GGYRCC 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 2 cut(s) 348, 393
BsiSI CCGG 3 cut(s) 150, 349, 934
BslFI GGGAC 3 cut(s) 138, 443, 842
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 4 cut(s) 240, 505, 530, 1132
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 3 cut(s) 138, 443, 842
BsnI GGCC 4 cut(s) 331, 353, 463, 545
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 2 cut(s) 348, 393
Bsp120I GGGCCC 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 333, 394, 987
Bsp143I GATC 4 cut(s) 302, 423, 1232, 1381
Bsp1720I GCTNAGC 1 cut(s) 918
BspACI CCGC 4 cut(s) 354, 369, 542, 546
BspANI GGCC 4 cut(s) 331, 353, 463, 545
BspCNI CTCAG 1 cut(s) 910
BspLI GGNNCC 6 cut(s) 330, 331, 332, 431, 432, 655
BspPI GGATC 3 cut(s) 310, 431, 1240
BspT107I GGYRCC 1 cut(s) 653
BspTNI GGTCTC 1 cut(s) 505
BsrDI GCAATG 3 cut(s) 12, 1128, 1238
BsrI ACTGG 2 cut(s) 229, 500
BssECI CCNNGG 3 cut(s) 121, 348, 792
BssMI GATC 4 cut(s) 302, 423, 1232, 1381
BssT1I CCWWGG 1 cut(s) 792
Bst2UI CCWGG 3 cut(s) 122, 172, 516
Bst4CI ACNGT 4 cut(s) 242, 490, 662, 1091
Bst6I CTCTTC 2 cut(s) 119, 961
BstAPI GCANNNNNTGC 2 cut(s) 554, 1454
BstC8I GCNNGC 3 cut(s) 73, 140, 1410
BstDEI CTNAG 4 cut(s) 588, 749, 918, 1212
BstF5I GGATG 3 cut(s) 108, 724, 1258
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 2 cut(s) 155, 872
BstKTI GATC 4 cut(s) 305, 426, 1235, 1384
BstMAI GTCTC 4 cut(s) 240, 505, 530, 1132
BstMBI GATC 4 cut(s) 302, 423, 1232, 1381
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 5 cut(s) 281, 409, 551, 554, 1454
BstNI CCWGG 3 cut(s) 122, 172, 516
BstNSI RCATGY 3 cut(s) 1066, 1180, 1419
BstPAI GACNNNNGTC 1 cut(s) 130
BstSCI CCNGG 5 cut(s) 120, 170, 347, 348, 514
BstSFI CTRYAG 2 cut(s) 658, 1431
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 142, 541, 724, 984
BstV2I GAAGAC 2 cut(s) 3, 474
BstX2I RGATCY 1 cut(s) 1232
BstYI RGATCY 1 cut(s) 1232
BstZI CGGCCG 1 cut(s) 543
BsuI GTATCC 2 cut(s) 224, 704
BsuRI GGCC 4 cut(s) 331, 353, 463, 545
BtsCI GGATG 3 cut(s) 108, 724, 1258
BtsI GCAGTG 2 cut(s) 279, 879
BtsIMutI CAGTG 4 cut(s) 279, 667, 879, 1169
Cac8I GCNNGC 3 cut(s) 73, 140, 1410
CaiI CAGNNNCTG 1 cut(s) 1409
CciNI GCGGCCGC 1 cut(s) 543
CfoI GCGC 2 cut(s) 155, 872
Cfr9I CCCGGG 1 cut(s) 348
Csp6I GTAC 3 cut(s) 493, 646, 1060
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 7 cut(s) 297, 472, 741, 1063, 1177, 1186, 1416
CviQI GTAC 3 cut(s) 493, 646, 1060
DdeI CTNAG 4 cut(s) 588, 749, 918, 1212
DpnI GATC 4 cut(s) 304, 425, 1234, 1383
DpnII GATC 4 cut(s) 302, 423, 1232, 1381
EaeI YGGCCR 1 cut(s) 543
EagI CGGCCG 1 cut(s) 543
Eam1104I CTCTTC 2 cut(s) 119, 961
EarI CTCTTC 2 cut(s) 119, 961
EclXI CGGCCG 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 2 cut(s) 333, 987
Eco31I GGTCTC 1 cut(s) 505
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 6 cut(s) 177, 366, 430, 1199, 1352, 1439
Eco52I CGGCCG 1 cut(s) 543
Eco88I CYCGRG 2 cut(s) 348, 393
EcoO109I RGGNCCY 3 cut(s) 329, 330, 430
EcoRI GAATTC 1 cut(s) 1218
EcoRII CCWGG 3 cut(s) 120, 170, 514
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 792
EcoT22I ATGCAT 1 cut(s) 1421
EcoT38I GRGCYC 2 cut(s) 333, 987
ErhI CCWWGG 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 7 cut(s) 300, 475, 744, 1066, 1180, 1189, 1419
FaqI GGGAC 3 cut(s) 138, 443, 842
FatI CATG 7 cut(s) 296, 471, 740, 1062, 1176, 1185, 1415
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 7 cut(s) 156, 354, 543, 546, 555, 738, 998
FokI GGATG 3 cut(s) 95, 731, 1245
FriOI GRGCYC 2 cut(s) 333, 987
Fsp4HI GCNGC 7 cut(s) 156, 354, 543, 546, 555, 738, 998
FspBI CTAG 3 cut(s) 507, 725, 1331
GlaI GCGC 2 cut(s) 154, 871
GluI GCNGC 7 cut(s) 156, 354, 543, 546, 555, 738, 998
GsaI CCCAGC 2 cut(s) 163, 829
GsuI CTGGAG 3 cut(s) 193, 246, 552
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 4 cut(s) 331, 353, 463, 545
HapII CCGG 3 cut(s) 150, 349, 934
HhaI GCGC 2 cut(s) 155, 872
Hin1II CATG 7 cut(s) 300, 475, 744, 1066, 1180, 1189, 1419
Hin6I GCGC 2 cut(s) 153, 870
HinP1I GCGC 2 cut(s) 153, 870
HincII GTYRAC 2 cut(s) 203, 583
HindII GTYRAC 2 cut(s) 203, 583
HinfI GANTC 3 cut(s) 665, 806, 899
HpaII CCGG 3 cut(s) 150, 349, 934
HphI GGTGA 5 cut(s) 495, 801, 942, 1015, 1214
Hpy166II GTNNAC 7 cut(s) 203, 217, 442, 493, 583, 1147, 1165
Hpy188I TCNGA 8 cut(s) 196, 362, 523, 575, 1362, 1378, 1405, 1439
Hpy188III TCNNGA 3 cut(s) 186, 708, 784
Hpy8I GTNNAC 7 cut(s) 203, 217, 442, 493, 583, 1147, 1165
HpyAV CCTTC 1 cut(s) 389
HpyCH4III ACNGT 4 cut(s) 242, 490, 662, 1091
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 101, 638, 740, 1412, 1419, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 409, 551, 554, 1454
HpyF3I CTNAG 4 cut(s) 588, 749, 918, 1212
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 7 cut(s) 300, 475, 744, 1066, 1180, 1189, 1419
HspAI GCGC 2 cut(s) 153, 870
KflI GGGWCCC 1 cut(s) 430
Kzo9I GATC 4 cut(s) 302, 423, 1232, 1381
LmnI GCTCC 3 cut(s) 797, 867, 1337
Lsp1109I GCAGC 4 cut(s) 142, 541, 724, 984
LweI GCATC 1 cut(s) 82
MaeI CTAG 3 cut(s) 507, 725, 1331
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 4 cut(s) 754, 1007, 1021, 1105
MalI GATC 4 cut(s) 304, 425, 1234, 1383
MboI GATC 4 cut(s) 302, 423, 1232, 1381
MboII GAAGA 7 cut(s) 3, 106, 109, 479, 948, 1227, 1291
MfeI CAATTG 1 cut(s) 1465
MflI RGATCY 1 cut(s) 1232
MhlI GDGCHC 3 cut(s) 333, 394, 987
MlyI GAGTC 1 cut(s) 893
MmeI TCCRAC 4 cut(s) 1315, 1315, 1327, 1417
Mph1103I ATGCAT 1 cut(s) 1421
MseI TTAA 4 cut(s) 221, 608, 1086, 1121
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 825
MspI CCGG 3 cut(s) 150, 349, 934
MspR9I CCNGG 5 cut(s) 122, 172, 349, 350, 516
MunI CAATTG 1 cut(s) 1465
MvaI CCWGG 3 cut(s) 122, 172, 516
MwoI GCNNNNNNNGC 5 cut(s) 281, 409, 551, 554, 1454
NciI CCSGG 2 cut(s) 349, 350
NdeII GATC 4 cut(s) 302, 423, 1232, 1381
NlaIII CATG 7 cut(s) 300, 475, 744, 1066, 1180, 1189, 1419
NlaIV GGNNCC 6 cut(s) 330, 331, 332, 431, 432, 655
NmuCI GTSAC 2 cut(s) 1021, 1105
NotI GCGGCCGC 1 cut(s) 543
NsiI ATGCAT 1 cut(s) 1421
NspI RCATGY 3 cut(s) 1066, 1180, 1419
PaeR7I CTCGAG 1 cut(s) 393
PciI ACATGT 2 cut(s) 1062, 1176
PfeI GAWTC 2 cut(s) 665, 806
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 7 cut(s) 157, 355, 544, 547, 556, 739, 999
PleI GAGTC 1 cut(s) 893
PpsI GAGTC 1 cut(s) 893
PpuMI RGGWCCY 1 cut(s) 430
PscI ACATGT 2 cut(s) 1062, 1176
PshAI GACNNNNGTC 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 3 cut(s) 120, 170, 514
PspFI CCCAGC 2 cut(s) 159, 825
PspGI CCWGG 3 cut(s) 120, 170, 514
PspN4I GGNNCC 6 cut(s) 330, 331, 332, 431, 432, 655
PspOMI GGGCCC 1 cut(s) 329
PspPPI RGGWCCY 1 cut(s) 430
PspXI VCTCGAGB 1 cut(s) 393
PsrI GAACNNNNNNTAC 2 cut(s) 1166, 1198
PstNI CAGNNNCTG 1 cut(s) 1409
PsuI RGATCY 1 cut(s) 1232
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 825
RsaI GTAC 3 cut(s) 494, 647, 1061
RsaNI GTAC 3 cut(s) 493, 646, 1060
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 4 cut(s) 221, 608, 1086, 1121
SatI GCNGC 7 cut(s) 156, 354, 543, 546, 555, 738, 998
Sau3AI GATC 4 cut(s) 302, 423, 1232, 1381
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 5 cut(s) 122, 172, 349, 350, 516
SduI GDGCHC 3 cut(s) 333, 394, 987
SfaNI GCATC 1 cut(s) 82
SfcI CTRYAG 2 cut(s) 658, 1431
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 6 cut(s) 177, 366, 430, 1199, 1352, 1439
SlaI CTCGAG 1 cut(s) 393
SmaI CCCGGG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
SsiI CCGC 4 cut(s) 354, 369, 542, 546
SspMI CTAG 3 cut(s) 507, 725, 1331
StyD4I CCNGG 5 cut(s) 120, 170, 347, 348, 514
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 4 cut(s) 242, 490, 662, 1091
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 394, 484
TatI WGTACW 3 cut(s) 492, 645, 1059
TauI GCSGC 3 cut(s) 356, 545, 548
TfiI GAWTC 2 cut(s) 665, 806
Tru1I TTAA 4 cut(s) 221, 608, 1086, 1121
Tru9I TTAA 4 cut(s) 221, 608, 1086, 1121
TscAI CASTG 4 cut(s) 286, 667, 879, 1176
TseFI GTSAC 2 cut(s) 1021, 1105
TseI GCWGC 4 cut(s) 155, 554, 737, 997
Tsp45I GTSAC 2 cut(s) 1021, 1105
TspDTI ATGAA 2 cut(s) 1227, 1296
TspMI CCCGGG 1 cut(s) 348
TspRI CASTG 4 cut(s) 286, 667, 879, 1176
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 6 cut(s) 177, 366, 430, 1199, 1352, 1439
XapI RAATTY 4 cut(s) 257, 836, 892, 1218
XceI RCATGY 3 cut(s) 1066, 1180, 1419
XcmI CCANNNNNNNNNTGG 1 cut(s) 1141
XhoI CTCGAG 1 cut(s) 393
XmaI CCCGGG 1 cut(s) 348
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 3 cut(s) 507, 725, 1331
Zsp2I ATGCAT 1 cut(s) 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.