FvH4_2g00600

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006215
Physical Location & Seq
Reverse (-)
634890 .. 637080
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00600.t1

Sequence Viewer

Length: 1503 bp
ATGGCAATGTCGAGAACCACTACTCTGTCTTTCTTCTCCCTCTTCATCTGTGTATTGATATCACTTGGCTCCACTATTGGTAATGTCCAATTAGTGCAGGCAGGCAACCCAAATTACAGAGATGCTCTTGCAAAGTCCATTCTCTTCTTCCATGGACAGAGGTCAGGGAAGCTCCCCTCCGGCGCCGCCCAACAGATGACTTGGAGGTCTAATTCTGGCCTTTCCGATGGCCGTCTCGCGCAAGTGGACTTAACTGGAGGATACTATGATGCTGGAGACAATGTCAAGTTCAACTTGCCAATGGCCTTCACCACCACAATGCTTTCATGGGGAGCACTTGAATATGGCGAGAAAATGGGACCTCAACTAGCAAGCACCCAGGAGGCAATCCGATGGTCCGCAACATATCTTCTAAAATGTGCTACAGCAACGCCCGGTAAGCTCTATGTTGGGGTCGGGGACCCCAACGCGGACCACAAGTGTTGGGTCAGGCCAGAAGACATGGACCAGGCCCGAACTGTGTACTCTGTCTCTTCAAGCAATCCGGGTTCGGATGTTGCTGGAGAGACCGCAGCCGCACTTGCGGCTGCTTCTATGGTTTTTCGAAAGGTAGATCCCAAATATTCCGCGTTGCTATTGAGCACAGCCAAGAAGGTGTTTCAATTTGCAATTCAGTACAGAGGTTCCTATAGTGATTCACTTGGTTCTGCAGTTTGCCCATTTTACTGCTCATATTCTGGATTCAAGGATGAGTTGATGTGGGGTGCTGCTTGGCTTCTTACAGCAACAAATGACGCTTTTTACTCCAATTTCTTGAATTCCATGGGAGGTGGTGATTCACCAGATATCTTTAGCTGGGATAACAAATATGCTGGTGCTTATGTGCTGCTATCAAGGAAAGCAATCTTGAGCAATGACAAAAAATTTGACTCATATAAACAACAAGCTGAGCAATTTATGTGTCGGATTTTGCCCAACTCACCCTCTTCAAGTACACAATACACACAAGGAGGGCTCATATACAAGCTGCCTGGAAGTAACCTCCAATATGTCACCTCAATCACATTCTTGCTTACTACCTATTCAAAACTCATGTCTTCCACAAAGCACACATTTAACTGTGGAAACCTTGTTGTCACTCCTAGCGCTTTAAGTAGCCTTGCCAAGAGACAGGTGGATTACATATTAGGGGAAAACCCTAAGAGAATGTCCTACATGGTAGGGTATGGAGCGAACTATCCTAAGAGAATTCACCACAGAGGATCTTCATTGCCCTCATTGGCAACTCATCCACAACGCATAGGTTGTGATGCTGGATTTCAGTTCTTCTATTCATCAAATCCAAATCCTAACATCTTAGTCGGAGCTGTTGTTGGAGGACCAGATCAGAATGATGGGTTTCCAGATGATCGCAATGATTATAGTCATTCAGAGCCTGCAACATACATAAATGGTGCTCTAGTTGGATCGTTAGCGTACTTTGCTGGAGGAAATCTCAACTAG

Protein Analysis

501

Amino Acids

54.31

Weight (kDa)

8.69

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 39 - 490 6.3e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 182
AccII CGCG 3 cut(s) 239, 470, 629
AciI CCGC 7 cut(s) 186, 399, 470, 570, 576, 584, 627
AclWI GGATC 3 cut(s) 608, 1270, 1474
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 3 cut(s) 817, 923, 1248
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 4 cut(s) 524, 677, 994, 1478
AfeI AGCGCT 1 cut(s) 1147
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 8 cut(s) 292, 341, 537, 662, 745, 817, 990, 1086
AjnI CCWGG 3 cut(s) 378, 507, 1030
AluBI AGCT 6 cut(s) 172, 442, 855, 947, 1027, 1367
AluI AGCT 6 cut(s) 172, 442, 855, 947, 1027, 1367
Alw21I GWGCWC 3 cut(s) 337, 644, 1459
Alw26I GTCTC 5 cut(s) 239, 270, 535, 560, 1162
AlwI GGATC 3 cut(s) 608, 1270, 1474
AlwNI CAGNNNCTG 1 cut(s) 1436
Aor51HI AGCGCT 1 cut(s) 1147
AoxI GGCC 5 cut(s) 217, 229, 303, 491, 510
ApeKI GCWGC 5 cut(s) 572, 587, 767, 886, 1027
ApoI RAATTY 3 cut(s) 817, 923, 1248
ArsI GACNNNNNNTTYG 4 cut(s) 908, 940, 1079, 1111
AspLEI GCGC 3 cut(s) 185, 241, 1148
AspS9I GGNCC 7 cut(s) 359, 396, 460, 472, 505, 511, 1379
AsuC2I CCSGG 2 cut(s) 435, 546
AsuHPI GGTGA 6 cut(s) 301, 831, 845, 972, 1045, 1244
AsuII TTCGAA 1 cut(s) 604
AvaII GGWCC 6 cut(s) 359, 396, 460, 472, 505, 1379
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 1017
BbsI GAAGAC 2 cut(s) 504, 1089
Bbv12I GWGCWC 3 cut(s) 337, 644, 1459
BbvI GCAGC 5 cut(s) 574, 584, 754, 873, 1014
BccI CCATC 3 cut(s) 221, 387, 1388
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 3 cut(s) 380, 509, 1032
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 2 cut(s) 435, 546
BcoDI GTCTC 5 cut(s) 239, 270, 535, 560, 1162
BfaI CTAG 4 cut(s) 368, 1143, 1460, 1501
BfmI CTRYAG 3 cut(s) 423, 688, 708
BfoI RGCGCY 2 cut(s) 186, 1149
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 8 cut(s) 186, 573, 576, 585, 588, 768, 887, 1028
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 8 cut(s) 187, 574, 577, 586, 589, 769, 888, 1029
Bme1390I CCNGG 5 cut(s) 380, 435, 509, 546, 1032
Bme18I GGWCC 6 cut(s) 359, 396, 460, 472, 505, 1379
BmgT120I GGNCC 7 cut(s) 359, 396, 460, 472, 505, 511, 1379
BmiI GGNNCC 6 cut(s) 70, 184, 360, 461, 462, 685
BmrFI CCNGG 5 cut(s) 380, 435, 509, 546, 1032
BmsI GCATC 3 cut(s) 112, 259, 1300
BoxI GACNNNNGTC 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 504, 1089
BplI GAGNNNNNCTC 1 cut(s) 1479
BpmI CTGGAG 3 cut(s) 276, 294, 582
Bpu1102I GCTNAGC 1 cut(s) 948
Bpu14I TTCGAA 1 cut(s) 604
BpuEI CTTGAG 1 cut(s) 928
BpuMI CCSGG 2 cut(s) 435, 546
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 151, 378, 822
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 1356, 1386
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 4 cut(s) 12, 919, 1268, 1420
BseBI CCWGG 3 cut(s) 380, 509, 1032
BseDI CCNNGG 3 cut(s) 151, 378, 822
BseGI GGATG 3 cut(s) 559, 754, 1288
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMI GCAATG 4 cut(s) 12, 919, 1268, 1420
BseMII CTCAG 1 cut(s) 939
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 5 cut(s) 574, 584, 754, 873, 1014
BseYI CCCAGC 1 cut(s) 855
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 3 cut(s) 239, 470, 629
BshFI GGCC 5 cut(s) 219, 231, 305, 493, 512
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 3 cut(s) 337, 644, 1459
BsiSI CCGG 3 cut(s) 180, 435, 545
BslFI GGGAC 2 cut(s) 372, 473
BslI CCNNNNNNNGG 1 cut(s) 469
BsmAI GTCTC 5 cut(s) 239, 270, 535, 560, 1162
BsmBI CGTCTC 1 cut(s) 239
BsmFI GGGAC 2 cut(s) 372, 473
BsnI GGCC 5 cut(s) 219, 231, 305, 493, 512
Bso31I GGTCTC 1 cut(s) 560
Bsp119I TTCGAA 1 cut(s) 604
Bsp1286I GDGCHC 4 cut(s) 337, 644, 1017, 1459
Bsp143I GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
Bsp1720I GCTNAGC 1 cut(s) 948
Bsp19I CCATGG 2 cut(s) 151, 822
BspACI CCGC 7 cut(s) 186, 399, 470, 570, 576, 584, 627
BspANI GGCC 5 cut(s) 219, 231, 305, 493, 512
BspCNI CTCAG 1 cut(s) 940
BspFNI CGCG 3 cut(s) 239, 470, 629
BspLI GGNNCC 6 cut(s) 70, 184, 360, 461, 462, 685
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 3 cut(s) 608, 1270, 1474
BspT104I TTCGAA 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 4 cut(s) 12, 919, 1268, 1420
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 151, 378, 822
BssMI GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 151, 822
Bst2UI CCWGG 3 cut(s) 380, 509, 1032
Bst4CI ACNGT 2 cut(s) 520, 1121
Bst6I CTCTTC 4 cut(s) 47, 149, 538, 991
BstACI GRCGYC 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 4 cut(s) 99, 103, 373, 1437
BstDEI CTNAG 4 cut(s) 948, 1200, 1242, 1357
BstDSI CCRYGG 2 cut(s) 151, 822
BstF5I GGATG 3 cut(s) 559, 754, 1288
BstFNI CGCG 3 cut(s) 239, 470, 629
BstH2I RGCGCY 2 cut(s) 186, 1149
BstHHI GCGC 3 cut(s) 185, 241, 1148
BstKTI GATC 5 cut(s) 616, 1265, 1387, 1411, 1469
BstMAI GTCTC 5 cut(s) 239, 270, 535, 560, 1162
BstMBI GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
BstMWI GCNNNNNNNGC 4 cut(s) 439, 581, 584, 1481
BstNI CCWGG 3 cut(s) 380, 509, 1032
BstPAI GACNNNNGTC 1 cut(s) 160
BstSCI CCNGG 5 cut(s) 378, 433, 507, 544, 1030
BstSFI CTRYAG 3 cut(s) 423, 688, 708
BstUI CGCG 3 cut(s) 239, 470, 629
BstV1I GCAGC 5 cut(s) 574, 584, 754, 873, 1014
BstV2I GAAGAC 2 cut(s) 504, 1089
BstX2I RGATCY 2 cut(s) 613, 1262
BstYI RGATCY 2 cut(s) 613, 1262
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 5 cut(s) 219, 231, 305, 493, 512
BtgI CCRYGG 2 cut(s) 151, 822
BtsCI GGATG 3 cut(s) 559, 754, 1288
Cac8I GCNNGC 4 cut(s) 99, 103, 373, 1437
CaiI CAGNNNCTG 1 cut(s) 1436
CfoI GCGC 3 cut(s) 185, 241, 1148
Cfr13I GGNCC 7 cut(s) 359, 396, 460, 472, 505, 511, 1379
CseI GACGC 1 cut(s) 803
Csp6I GTAC 4 cut(s) 523, 676, 993, 1477
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CviAII CATG 6 cut(s) 152, 327, 502, 823, 1093, 1216
CviQI GTAC 4 cut(s) 523, 676, 993, 1477
DdeI CTNAG 4 cut(s) 948, 1200, 1242, 1357
DinI GGCGCC 1 cut(s) 184
DpnI GATC 5 cut(s) 615, 1264, 1386, 1410, 1468
DpnII GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 4 cut(s) 47, 149, 538, 991
EarI CTCTTC 4 cut(s) 47, 149, 538, 991
Eco130I CCWWGG 2 cut(s) 151, 822
Eco24I GRGCYC 1 cut(s) 1017
Eco31I GGTCTC 1 cut(s) 560
Eco32I GATATC 2 cut(s) 60, 847
Eco47I GGWCC 6 cut(s) 359, 396, 460, 472, 505, 1379
Eco47III AGCGCT 1 cut(s) 1147
EcoO109I RGGNCCY 2 cut(s) 359, 460
EcoRI GAATTC 2 cut(s) 817, 1248
EcoRII CCWGG 3 cut(s) 378, 507, 1030
EcoRV GATATC 2 cut(s) 60, 847
EcoT14I CCWWGG 2 cut(s) 151, 822
EcoT38I GRGCYC 1 cut(s) 1017
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 151, 822
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 6 cut(s) 155, 330, 505, 826, 1096, 1219
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 2 cut(s) 372, 473
FatI CATG 6 cut(s) 151, 326, 501, 822, 1092, 1215
Fnu4HI GCNGC 8 cut(s) 186, 573, 576, 585, 588, 768, 887, 1028
FokI GGATG 3 cut(s) 566, 761, 1275
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 8 cut(s) 186, 573, 576, 585, 588, 768, 887, 1028
FspBI CTAG 4 cut(s) 368, 1143, 1460, 1501
GlaI GCGC 3 cut(s) 184, 240, 1147
GluI GCNGC 8 cut(s) 186, 573, 576, 585, 588, 768, 887, 1028
GsaI CCCAGC 1 cut(s) 859
GsuI CTGGAG 3 cut(s) 276, 294, 582
HaeII RGCGCY 2 cut(s) 186, 1149
HaeIII GGCC 5 cut(s) 219, 231, 305, 493, 512
HapII CCGG 3 cut(s) 180, 435, 545
HgaI GACGC 1 cut(s) 803
HhaI GCGC 3 cut(s) 185, 241, 1148
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 6 cut(s) 155, 330, 505, 826, 1096, 1219
Hin6I GCGC 3 cut(s) 183, 239, 1146
HinP1I GCGC 3 cut(s) 183, 239, 1146
HinfI GANTC 4 cut(s) 695, 741, 836, 929
HpaII CCGG 3 cut(s) 180, 435, 545
HphI GGTGA 6 cut(s) 301, 831, 845, 972, 1045, 1244
Hpy166II GTNNAC 3 cut(s) 247, 523, 995
Hpy188I TCNGA 7 cut(s) 226, 392, 553, 966, 1364, 1389, 1432
Hpy188III TCNNGA 5 cut(s) 12, 738, 814, 907, 1403
Hpy8I GTNNAC 3 cut(s) 247, 523, 995
HpyAV CCTTC 2 cut(s) 316, 646
HpyCH4III ACNGT 2 cut(s) 520, 1121
HpyCH4V TGCA 5 cut(s) 97, 131, 668, 710, 1439
HpyF10VI GCNNNNNNNGC 4 cut(s) 439, 581, 584, 1481
HpyF3I CTNAG 4 cut(s) 948, 1200, 1242, 1357
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 6 cut(s) 155, 330, 505, 826, 1096, 1219
HspAI GCGC 3 cut(s) 183, 239, 1146
KasI GGCGCC 1 cut(s) 182
KflI GGGWCCC 1 cut(s) 460
Kzo9I GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
LmnI GCTCC 5 cut(s) 74, 177, 332, 1229, 1364
Lsp1109I GCAGC 5 cut(s) 574, 584, 754, 873, 1014
LweI GCATC 3 cut(s) 112, 259, 1300
MaeI CTAG 4 cut(s) 368, 1143, 1460, 1501
MaeIII GTNAC 3 cut(s) 1037, 1051, 1135
MalI GATC 5 cut(s) 615, 1264, 1386, 1410, 1468
MboI GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
MflI RGATCY 2 cut(s) 613, 1262
MhlI GDGCHC 4 cut(s) 337, 644, 1017, 1459
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 4 cut(s) 944, 1342, 1354, 1444
MseI TTAA 3 cut(s) 251, 1116, 1151
MslI CAYNNNNRTG 1 cut(s) 317
MspI CCGG 3 cut(s) 180, 435, 545
MspR9I CCNGG 5 cut(s) 380, 435, 509, 546, 1032
MvaI CCWGG 3 cut(s) 380, 509, 1032
MvnI CGCG 3 cut(s) 239, 470, 629
MwoI GCNNNNNNNGC 4 cut(s) 439, 581, 584, 1481
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 2 cut(s) 435, 546
NcoI CCATGG 2 cut(s) 151, 822
NdeII GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
NlaIII CATG 6 cut(s) 155, 330, 505, 826, 1096, 1219
NlaIV GGNNCC 6 cut(s) 70, 184, 360, 461, 462, 685
NmuCI GTSAC 2 cut(s) 1051, 1135
NspV TTCGAA 1 cut(s) 604
PfeI GAWTC 3 cut(s) 695, 741, 836
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 8 cut(s) 187, 574, 577, 586, 589, 769, 888, 1029
PleI GAGTC 1 cut(s) 923
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 923
PpuMI RGGWCCY 2 cut(s) 359, 460
PshAI GACNNNNGTC 1 cut(s) 160
Psp5II RGGWCCY 2 cut(s) 359, 460
Psp6I CCWGG 3 cut(s) 378, 507, 1030
PspFI CCCAGC 1 cut(s) 855
PspGI CCWGG 3 cut(s) 378, 507, 1030
PspN4I GGNNCC 6 cut(s) 70, 184, 360, 461, 462, 685
PspPI GGNCC 7 cut(s) 359, 396, 460, 472, 505, 511, 1379
PspPPI RGGWCCY 2 cut(s) 359, 460
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 2 cut(s) 613, 1262
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 4 cut(s) 524, 677, 994, 1478
RsaNI GTAC 4 cut(s) 523, 676, 993, 1477
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 3 cut(s) 251, 1116, 1151
SatI GCNGC 8 cut(s) 186, 573, 576, 585, 588, 768, 887, 1028
Sau3AI GATC 5 cut(s) 613, 1262, 1384, 1408, 1466
Sau96I GGNCC 7 cut(s) 359, 396, 460, 472, 505, 511, 1379
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 5 cut(s) 380, 435, 509, 546, 1032
SduI GDGCHC 4 cut(s) 337, 644, 1017, 1459
SfaNI GCATC 3 cut(s) 112, 259, 1300
SfcI CTRYAG 3 cut(s) 423, 688, 708
SfoI GGCGCC 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 6 cut(s) 359, 396, 460, 472, 505, 1379
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
SsiI CCGC 7 cut(s) 186, 399, 470, 570, 576, 584, 627
SspDI GGCGCC 1 cut(s) 182
SspI AATATT 1 cut(s) 623
SspMI CTAG 4 cut(s) 368, 1143, 1460, 1501
StyD4I CCNGG 5 cut(s) 378, 433, 507, 544, 1030
StyI CCWWGG 2 cut(s) 151, 822
TaaI ACNGT 2 cut(s) 520, 1121
TaqI TCGA 2 cut(s) 11, 604
TatI WGTACW 3 cut(s) 522, 675, 992
TauI GCSGC 3 cut(s) 188, 578, 587
TfiI GAWTC 3 cut(s) 695, 741, 836
Tru1I TTAA 3 cut(s) 251, 1116, 1151
Tru9I TTAA 3 cut(s) 251, 1116, 1151
TseFI GTSAC 2 cut(s) 1051, 1135
TseI GCWGC 5 cut(s) 572, 587, 767, 886, 1027
Tsp45I GTSAC 2 cut(s) 1051, 1135
TspDTI ATGAA 4 cut(s) 34, 315, 1257, 1323
Tth111I GACNNNGTC 1 cut(s) 281
VpaK11BI GGWCC 6 cut(s) 359, 396, 460, 472, 505, 1379
XapI RAATTY 3 cut(s) 817, 923, 1248
XcmI CCANNNNNNNNNTGG 1 cut(s) 1171
XspI CTAG 4 cut(s) 368, 1143, 1460, 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.