FvH4_2g02920

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015529
Physical Location & Seq
Forward (+)
2280341 .. 2281574
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02920.t1

Sequence Viewer

Length: 510 bp
ATGGAGTTGTGTAAGCTTTCATCGGTCCTCATTTCTCTCGTAGCCTTATTATCCCTACTCCATTCCCTTTACGCGCAGGACTCAATCCAAGACTACCTGAACAGCCACAACTTTGCTCGGTTGGCGGTCGGTAACGTTCCGATGGAGTGGGATCCTGAACTAGAGCGTTTTGCCCAAGACTATGCCTCAAAGCACGTCCATGACTGCCAACTTGTCCACTCCGGTCATAAGTACGGTGAAAACCTTGCCATGAGCTGGGGTGAGATGTTCGGCTTAGATGCTGTGAGTATGTGGGTAGGGGAGAAGCAGAACTACGATTACGCCTCGAATTCTTGCGTCGGCGGCGAATGCGGGCATTATTTGCAGGTCATTTGGAACACCTCGACTCATCTGGGTTGTGCCAAAGCGAGGTGCAAGAACGGGGGTACTTGGATCGGCTGCAACTATAACCCCCCTCCAAGTGGGGATAGGCCTTACAACCCCCCGCCAAGTGAGGAGAGCCTTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

170

Amino Acids

18.85

Weight (kDa)

5.35

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 33 - 149 2.5e-16 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AccB7I CCANNNNNTGG 1 cut(s) 255
AccII CGCG 1 cut(s) 74
AciI CCGC 4 cut(s) 125, 342, 351, 485
AclI AACGTT 1 cut(s) 135
AclWI GGATC 3 cut(s) 146, 159, 440
AcsI RAATTY 1 cut(s) 328
AfaI GTAC 2 cut(s) 233, 427
AfiI CCNNNNNNNGG 3 cut(s) 255, 408, 461
AjiI CACGTC 1 cut(s) 196
AluBI AGCT 2 cut(s) 16, 255
AluI AGCT 2 cut(s) 16, 255
AlwI GGATC 3 cut(s) 146, 159, 440
AoxI GGCC 1 cut(s) 470
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 1 cut(s) 328
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 248, 272
AvaII GGWCC 1 cut(s) 25
BamHI GGATCC 1 cut(s) 151
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbvI GCAGC 1 cut(s) 425
BccI CCATC 1 cut(s) 136
BfaI CTAG 1 cut(s) 161
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 2 cut(s) 343, 439
BlsI GCNGC 2 cut(s) 344, 440
Bme18I GGWCC 1 cut(s) 25
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 153
BmsI GCATC 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 221
Bsc4I CCNNNNNNNGG 3 cut(s) 255, 408, 461
BseLI CCNNNNNNNGG 3 cut(s) 255, 408, 461
BseRI GAGGAG 1 cut(s) 509
BseXI GCAGC 1 cut(s) 425
BseYI CCCAGC 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 472
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 255, 408, 461
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 1 cut(s) 472
Bsp143I GATC 2 cut(s) 151, 432
BspACI CCGC 4 cut(s) 125, 342, 351, 485
BspANI GGCC 1 cut(s) 472
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 3 cut(s) 146, 159, 440
BssMI GATC 2 cut(s) 151, 432
Bst4CI ACNGT 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 274
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 2 cut(s) 154, 435
BstMBI GATC 2 cut(s) 151, 432
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 4 cut(s) 122, 342, 348, 361
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 472
BtrI CACGTC 1 cut(s) 196
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 232, 426
CviAII CATG 2 cut(s) 200, 250
CviJI RGCY 8 cut(s) 16, 44, 105, 255, 273, 438, 472, 501
CviKI_1 RGCY 8 cut(s) 16, 44, 105, 255, 273, 438, 472, 501
CviQI GTAC 2 cut(s) 232, 426
DdeI CTNAG 1 cut(s) 274
DpnI GATC 2 cut(s) 153, 434
DpnII GATC 2 cut(s) 151, 432
Eco147I AGGCCT 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 25
EcoRI GAATTC 1 cut(s) 328
FaeI CATG 2 cut(s) 203, 253
FaiI YATR 6 cut(s) 183, 201, 228, 251, 290, 447
FatI CATG 2 cut(s) 199, 249
FauI CCCGC 2 cut(s) 344, 492
Fnu4HI GCNGC 2 cut(s) 343, 439
Fsp4HI GCNGC 2 cut(s) 343, 439
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 343, 439
GsaI CCCAGC 1 cut(s) 259
HaeIII GGCC 1 cut(s) 472
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 325
HhaI GCGC 1 cut(s) 76
Hin1II CATG 2 cut(s) 203, 253
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 2 cut(s) 80, 385
HpaII CCGG 1 cut(s) 222
HphI GGTGA 2 cut(s) 248, 272
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 1 cut(s) 341
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 2 cut(s) 135, 195
HpyCH4V TGCA 3 cut(s) 364, 414, 441
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 342, 348, 361
HpyF3I CTNAG 1 cut(s) 274
HpySE526I ACGT 2 cut(s) 135, 195
Hsp92II CATG 2 cut(s) 203, 253
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 2 cut(s) 151, 432
LpnPI CCDG 7 cut(s) 62, 110, 168, 235, 241, 350, 377
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 161
MaeII ACGT 2 cut(s) 135, 195
MaeIII GTNAC 1 cut(s) 131
MalI GATC 2 cut(s) 153, 434
MboI GATC 2 cut(s) 151, 432
MflI RGATCY 1 cut(s) 151
MluCI AATT 1 cut(s) 328
MlyI GAGTC 2 cut(s) 74, 379
MnlI CCTC 7 cut(s) 38, 196, 334, 391, 402, 465, 487
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 353
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 4 cut(s) 122, 342, 348, 361
NdeII GATC 2 cut(s) 151, 432
NlaIII CATG 2 cut(s) 203, 253
NlaIV GGNNCC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 472
PctI GAATGC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 2 cut(s) 344, 440
PleI GAGTC 2 cut(s) 74, 379
PpsI GAGTC 2 cut(s) 74, 379
Psp1406I AACGTT 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 25
PsrI GAACNNNNNNTAC 2 cut(s) 302, 334
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 2 cut(s) 233, 427
RsaNI GTAC 2 cut(s) 232, 426
RseI CAYNNNNRTG 1 cut(s) 198
SatI GCNGC 2 cut(s) 343, 439
Sau3AI GATC 2 cut(s) 151, 432
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 2 cut(s) 74, 379
SetI ASST 9 cut(s) 18, 99, 138, 198, 246, 257, 369, 383, 413
SfaNI GCATC 1 cut(s) 268
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 1 cut(s) 328
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 4 cut(s) 125, 342, 351, 485
SspMI CTAG 1 cut(s) 161
StuI AGGCCT 1 cut(s) 472
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 2 cut(s) 138, 198
TaqI TCGA 2 cut(s) 326, 383
TaqII GACCGA 1 cut(s) 13
TasI AATT 1 cut(s) 328
TauI GCSGC 1 cut(s) 345
TseI GCWGC 1 cut(s) 438
TspDTI ATGAA 1 cut(s) 9
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 1 cut(s) 328
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.