RchiOBHm_Chr6g0247701

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015529
Physical Location & Seq
Forward (+)
3729516 .. 3730022
507 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22203

Sequence Viewer

Length: 507 bp
ATGGAATTGTGCAAGATTTCATCAGTCCTGATTTCTCTCGTAGCCTTATCCCTGCTCCATTCCATTTTTGCGCAAGACTCAATCAAAGACTACCTGAACGGCCACAACTTTGCTCGAGCGGCGGTAGGTAACGGGCCCATGGTGTGGGATGATGAACTAGCGCGTTTTGCTCAAGACTATGCTGCAAAACACACTCATGACTGCCAACTTGTCCACTCCGGTTATAAATACGGTGAAAACCTTGCCATGAGTTGGGGTGAGATGTTCGGCTTAGATGCTGTGAGCATGTGGGTGGGGGAGAAGCCGAATTACGATTACAACTCGAATTCTTGCGTTGGTGGCGAATGTGGACATTATTTGCAGGTCATTTGGAACACCTCGACTCATCTGGGGTGTGCCAAAGCAAGGTGCAACAATGGGGGTACTTGGATTGGGTGCAACTATAATCCCCCGCCAAGTGGGGATAGGCCTTATAATCCCCCGCCACGTGGAGATAGGCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

168

Amino Acids

18.58

Weight (kDa)

5.92

Isoelectric Point (pI)

23.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 32 - 148 8.1e-17 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 225, 474
Acc16I TGCGCA 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 352
AccB7I CCANNNNNTGG 2 cut(s) 144, 252
AccBSI CCGCTC 1 cut(s) 119
AccII CGCG 1 cut(s) 163
AciI CCGC 4 cut(s) 119, 122, 452, 482
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 325
AcvI CACGTG 1 cut(s) 488
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 5 cut(s) 144, 252, 405, 458, 488
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 4 cut(s) 100, 134, 467, 497
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 325
AspLEI GCGC 2 cut(s) 73, 163
AspS9I GGNCC 2 cut(s) 134, 135
AsuHPI GGTGA 2 cut(s) 245, 269
AvaI CYCGRG 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
BbrPI CACGTG 1 cut(s) 488
BbvI GCAGC 1 cut(s) 169
BceAI ACGGC 1 cut(s) 115
BfaI CTAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 2 cut(s) 120, 183
BlsI GCNGC 2 cut(s) 121, 184
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 134, 135
BmiI GGNNCC 1 cut(s) 136
BmsI GCATC 1 cut(s) 265
BpuEI CTTGAG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 138
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 252, 405, 458, 488
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 5 cut(s) 144, 252, 405, 458, 488
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 102, 136, 469, 499
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 5 cut(s) 144, 252, 405, 458, 488
BsnI GGCC 4 cut(s) 102, 136, 469, 499
BsoBI CYCGRG 1 cut(s) 114
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 4 cut(s) 119, 122, 452, 482
BspANI GGCC 4 cut(s) 102, 136, 469, 499
BspFNI CGCG 1 cut(s) 163
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 352
BsrBI CCGCTC 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 233
BstBAI YACGTR 1 cut(s) 488
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 1 cut(s) 163
BstHHI GCGC 2 cut(s) 73, 163
BstMWI GCNNNNNNNGC 3 cut(s) 119, 167, 339
BstNSI RCATGY 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 169
BsuRI GGCC 4 cut(s) 102, 136, 469, 499
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 352
CciI TCATGA 1 cut(s) 196
CfoI GCGC 2 cut(s) 73, 163
Cfr13I GGNCC 2 cut(s) 134, 135
Csp6I GTAC 1 cut(s) 423
CviAII CATG 4 cut(s) 139, 197, 247, 286
CviJI RGCY 7 cut(s) 44, 102, 136, 270, 304, 469, 499
CviKI_1 RGCY 7 cut(s) 44, 102, 136, 270, 304, 469, 499
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 138
Eco147I AGGCCT 2 cut(s) 469, 499
Eco24I GRGCYC 1 cut(s) 138
Eco72I CACGTG 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 4 cut(s) 142, 200, 250, 289
FaiI YATR 8 cut(s) 140, 180, 198, 225, 248, 287, 444, 474
FatI CATG 4 cut(s) 138, 196, 246, 285
FauI CCCGC 2 cut(s) 459, 489
Fnu4HI GCNGC 2 cut(s) 120, 183
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 120, 183
FspBI CTAG 1 cut(s) 158
FspI TGCGCA 1 cut(s) 72
GlaI GCGC 2 cut(s) 72, 162
GluI GCNGC 2 cut(s) 120, 183
HaeIII GGCC 4 cut(s) 102, 136, 469, 499
HapII CCGG 1 cut(s) 219
HhaI GCGC 2 cut(s) 73, 163
Hin1II CATG 4 cut(s) 142, 200, 250, 289
Hin6I GCGC 2 cut(s) 71, 161
HinP1I GCGC 2 cut(s) 71, 161
HinfI GANTC 2 cut(s) 77, 382
HpaII CCGG 1 cut(s) 219
HphI GGTGA 2 cut(s) 245, 269
Hpy166II GTNNAC 2 cut(s) 214, 350
Hpy188III TCNNGA 3 cut(s) 28, 173, 197
Hpy8I GTNNAC 2 cut(s) 214, 350
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 5 cut(s) 12, 185, 361, 411, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 167, 339
HpyF3I CTNAG 1 cut(s) 271
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 4 cut(s) 142, 200, 250, 289
HspAI GCGC 2 cut(s) 71, 161
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 6 cut(s) 41, 65, 107, 232, 347, 374
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 5, 307, 325
MlyI GAGTC 2 cut(s) 71, 376
MnlI CCTC 1 cut(s) 388
MslI CAYNNNNRTG 2 cut(s) 195, 290
MspI CCGG 1 cut(s) 219
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 3 cut(s) 119, 167, 339
NcoI CCATGG 1 cut(s) 138
NlaIII CATG 4 cut(s) 142, 200, 250, 289
NlaIV GGNNCC 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 72
NspI RCATGY 1 cut(s) 289
PaeR7I CTCGAG 1 cut(s) 114
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 2 cut(s) 469, 499
PflMI CCANNNNNTGG 2 cut(s) 144, 252
PkrI GCNGC 2 cut(s) 121, 184
PleI GAGTC 2 cut(s) 71, 376
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 2 cut(s) 71, 376
Ppu21I YACGTR 1 cut(s) 488
PsiI TTATAA 2 cut(s) 225, 474
PspCI CACGTG 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 136
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 2 cut(s) 134, 135
PspXI VCTCGAGB 1 cut(s) 114
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 2 cut(s) 195, 290
SatI GCNGC 2 cut(s) 120, 183
Sau96I GGNCC 2 cut(s) 134, 135
SchI GAGTC 2 cut(s) 71, 376
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 96, 130, 243, 366, 380, 410, 490
SfaNI GCATC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 114
SlaI CTCGAG 1 cut(s) 114
SmiMI CAYNNNNRTG 2 cut(s) 195, 290
SmlI CTYRAG 2 cut(s) 114, 171
SmoI CTYRAG 2 cut(s) 114, 171
Sse9I AATT 3 cut(s) 5, 307, 325
SseBI AGGCCT 2 cut(s) 469, 499
SsiI CCGC 4 cut(s) 119, 122, 452, 482
SspMI CTAG 1 cut(s) 158
StuI AGGCCT 2 cut(s) 469, 499
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 115, 323, 380
TasI AATT 3 cut(s) 5, 307, 325
TauI GCSGC 1 cut(s) 122
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 9, 168
Van91I CCANNNNNTGG 2 cut(s) 144, 252
XapI RAATTY 1 cut(s) 325
XceI RCATGY 1 cut(s) 289
XhoI CTCGAG 1 cut(s) 114
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.