FvH4_2g04240

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013102
Physical Location & Seq
Reverse (-)
3311614 .. 3314650
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04240.t1

Sequence Viewer

Length: 1788 bp
ATGCCTAGCCTAGGTTCTGGGGTTCTTGAGAAGCTTATTGAAGAAATGCAAAGTGACTCACATGGTGATCCAAAGCCTGTGTTATTACAAATCAGAAACATCACGCCGGTTTTAGCAGAAGGTGATCTTTGGCCCAATAAAGGGTTTTACATGAAGGTTTCAGATGCCACACACACTATGTATGTGTCATTGCCTCCAGAGCAGGATGACATGGTTCTATGCAACACATTGCAATTAGGGCAATTGATATACGTCAAAAAGTTGGAGGAGGCGCGGCCGGTGCCTGTGCTTAGAGGTATAAACCCTGTTCCGGGCCGACTCCCCTGTGTTGGACACCATGAAGATCTCTTTTCCATTGATAAGTTTGTGGATTTTAAAGGGGTGTCTGATTTACTATTTGTAATGGAGAAAAGCAATGGCGTGGAGAAGAAGCTACAGCCAAAATTTCGATCTTTGAGGGCCTCTAAGATTCACCCAATCGAGAAGATCACAAGGGTATGCAGGTGTGGAAGTGGTGATGTTGTTGAGAAGGAAATATGTGAGATACCAAAAGGGTCGAGAAAAGTGAAATTAAGATGTGTAGAGAGTGATTCTGATAGCACGATATCTTCTTCCTCCTTAAGGAGAAGGAGCTGGACTGGACCCGAAGCTGCAGATTTCCCACTTGTTAAGAACATGATAAAGCCATCTGCTAGGCGCAGTCGCAGTGCGCATGTTTCTCCAGTTCGTTCTACAAGAAGTGTTTCAGATGACAATTCAAGTTGTAAAATAAAAAGAACAATCAGTGATGAAGTAGCTACAAAGTTAAGTAAAAACTCCAACAGTAGGATCTCAGTGTCGGCGAAAAATTGTGAGTTACCCTTAGATTCAGTGATGGTTAATTTGGCTGTTGATAGTAAATGTTCAGAAAGTAATATTCAGTGGAATTCCCTGCCTCCAATGTTAGTGACTCTTGGAAAGGAGGTGTTGAGGCACAAAGATGTTTCTATGCTTACTGCAGTGGAGGCTTTGCAAGAGGCTTCTGTTGCTGAGAGATTGCTAAAAAGCTTAAGTACATACTCAGAACTTCAGGCAACCGAAGGGGAGGAACACCATCCGTCAATCAATAAATTTTTTGATCTTCAAGACTACTTAGTTCATACTCAATTGATTGTGCAGTCATTGACCAACATCAGTCCGCTTAGAGATGATGACACAAATTCAGGCAACCCTGGTTCCGTCAAGGAAGCACTAATGCTTGCAGTACATCGAAAGAAAAATGCAATGTCATGGATTAAAGCAGCAATGACAAATGATCTATCCCCATCTTTTTCTCTTGATAATGTTTCAGTGAAGGCTACAAATCCATCAGATAAATCAAATAAAACTTATGGTAGTGTACCAAAGGGTACACTTATGGTTAAGAAGCCAAAGAATGATGCTCATTTAGGATTGGTCTCAAAGAGAGAAAACTCACACGATTGGGTGACGGGAAATGCTTTATCTGCAGTTGCAGACCTGGAAAAATCCTTGCATGATGAATGCAGAAGGTGGTTTTTAGCTACCTTTGAGAGTTACCTGGATGAAGCTAGGAGCAGAACTGATTCAATGGAATCTGATAGCCAAGTAGCCGAGACTATGTGTCTAATTAAGAAAGTGGGAGACTGGTTGGATGTGATTGTGAGCAAAGAAGATTCTGAATTGGAAGTTTATGGGAGGATCAAGGAGAAAATATATGGCGTACTTCTGAAGAATGTGGAGAGGACTACTATGGCTATGGAACAAATGAATGCAATATTCAATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

596

Amino Acids

66.24

Weight (kDa)

7.57

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF936 PF06075 5 - 117 1.5e-42 Plant protein of unknown function (DUF936)
DUF6857 PF21647 298 - 586 6.7e-73 Domain of unknown function (DUF6857)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19610
fragaria_vesca FvH4_2g04240
malus_domestica MD05G1099200.v1.1
prunus_persica Prupe.8G143800_v2.0.a1 Prupe.8G143800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0253661
rosa_laevigata RLG00000014995
rosa_multiflora Rmu_co8217522.1_g000001 Rmu_sc0001351.1_g000001
rosa_roxburghii Rroxscaffold_7G00211720
rosa_rugosa Rorug05G0545400
rosa_samantha Rh6AG061100 Rh6BG054900 Rh6CG054500 Rh6CG054600 Rh6DG052000 Rh6DG052100
rosa_wichuraiana Rw6G005430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 492
Acc16I TGCGCA 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 280
AccII CGCG 1 cut(s) 274
AciI CCGC 2 cut(s) 274, 1178
AclWI GGATC 3 cut(s) 62, 836, 1706
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 4 cut(s) 443, 925, 1109, 1198
AcuI CTGAAG 2 cut(s) 1052, 1748
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 5 cut(s) 1054, 1245, 1380, 1390, 1722
AfiI CCNNNNNNNGG 8 cut(s) 11, 140, 141, 310, 311, 329, 621, 825
AflII CTTAAG 2 cut(s) 619, 1048
AgsI TTSAA 5 cut(s) 41, 759, 1124, 1587, 1780
AhdI GACNNNNNGTC 1 cut(s) 1620
AjnI CCWGG 3 cut(s) 1210, 1497, 1557
AluBI AGCT 8 cut(s) 34, 433, 633, 650, 797, 1047, 1541, 1568
AluI AGCT 8 cut(s) 34, 433, 633, 650, 797, 1047, 1541, 1568
Alw26I GTCTC 3 cut(s) 1441, 1607, 1635
AlwI GGATC 3 cut(s) 62, 836, 1706
AoxI GGCC 4 cut(s) 131, 275, 313, 459
ApeKI GCWGC 2 cut(s) 650, 1280
ApoI RAATTY 4 cut(s) 443, 925, 1109, 1198
Asp700I GAANNNNTTC 2 cut(s) 742, 1582
AspA2I CCTAGG 1 cut(s) 10
AspLEI GCGC 3 cut(s) 274, 699, 712
AspS9I GGNCC 4 cut(s) 132, 313, 459, 641
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 5 cut(s) 77, 134, 464, 527, 1477
AvaII GGWCC 1 cut(s) 641
AvrII CCTAGG 1 cut(s) 10
BanI GGYRCC 1 cut(s) 280
BbvI GCAGC 2 cut(s) 637, 1292
BccI CCATC 5 cut(s) 694, 868, 1101, 1312, 1354
BciT130I CCWGG 3 cut(s) 1212, 1499, 1559
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 3 cut(s) 1441, 1607, 1635
BfaI CTAG 4 cut(s) 6, 11, 693, 1569
BfmI CTRYAG 4 cut(s) 434, 651, 996, 1485
BfrI CTTAAG 2 cut(s) 619, 1048
BfuAI ACCTGC 1 cut(s) 492
BglII AGATCT 1 cut(s) 343
BisI GCNGC 3 cut(s) 275, 651, 1281
BlnI CCTAGG 1 cut(s) 10
BlsI GCNGC 3 cut(s) 276, 652, 1282
Bme1390I CCNGG 4 cut(s) 312, 1212, 1499, 1559
Bme18I GGWCC 1 cut(s) 641
BmeRI GACNNNNNGTC 1 cut(s) 1620
BmgT120I GGNCC 4 cut(s) 132, 313, 459, 641
BmiI GGNNCC 3 cut(s) 282, 643, 1216
BmrFI CCNGG 4 cut(s) 312, 1212, 1499, 1559
BmsI GCATC 2 cut(s) 154, 1408
BpmI CTGGAG 2 cut(s) 180, 705
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 1441
BsaJI CCNNGG 2 cut(s) 10, 1210
Bsc4I CCNNNNNNNGG 8 cut(s) 11, 140, 141, 310, 311, 329, 621, 825
Bse118I RCCGGY 2 cut(s) 106, 277
Bse1I ACTGG 3 cut(s) 643, 722, 1649
Bse3DI GCAATG 5 cut(s) 188, 227, 421, 1269, 1290
BseBI CCWGG 3 cut(s) 1212, 1499, 1559
BseDI CCNNGG 2 cut(s) 10, 1210
BseGI GGATG 4 cut(s) 211, 1093, 1567, 1657
BseLI CCNNNNNNNGG 8 cut(s) 11, 140, 141, 310, 311, 329, 621, 825
BseMI GCAATG 5 cut(s) 188, 227, 421, 1269, 1290
BseMII CTCAG 3 cut(s) 846, 1020, 1074
BseNI ACTGG 3 cut(s) 643, 722, 1649
BseRI GAGGAG 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 637, 1292
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 4 cut(s) 133, 277, 315, 461
BshNI GGYRCC 1 cut(s) 280
BsiEI CGRYCG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 107, 278, 311
BslI CCNNNNNNNGG 8 cut(s) 11, 140, 141, 310, 311, 329, 621, 825
BsmAI GTCTC 3 cut(s) 1441, 1607, 1635
BsmI GAATGC 2 cut(s) 1526, 1774
BsnI GGCC 4 cut(s) 133, 277, 315, 461
Bso31I GGTCTC 1 cut(s) 1441
Bsp143I GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
BspACI CCGC 2 cut(s) 274, 1178
BspANI GGCC 4 cut(s) 133, 277, 315, 461
BspCNI CTCAG 3 cut(s) 845, 1021, 1073
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 3 cut(s) 282, 643, 1216
BspMAI CTGCAG 3 cut(s) 655, 1000, 1489
BspMI ACCTGC 1 cut(s) 492
BspPI GGATC 3 cut(s) 62, 836, 1706
BspT107I GGYRCC 1 cut(s) 280
BspTI CTTAAG 2 cut(s) 619, 1048
BspTNI GGTCTC 1 cut(s) 1441
BsrDI GCAATG 5 cut(s) 188, 227, 421, 1269, 1290
BsrFI RCCGGY 2 cut(s) 106, 277
BsrI ACTGG 3 cut(s) 643, 722, 1649
BssAI RCCGGY 2 cut(s) 106, 277
BssECI CCNNGG 2 cut(s) 10, 1210
BssMI GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
BssT1I CCWWGG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 1212, 1499, 1559
Bst4CI ACNGT 1 cut(s) 824
BstAFI CTTAAG 2 cut(s) 619, 1048
BstC8I GCNNGC 1 cut(s) 1239
BstDEI CTNAG 8 cut(s) 290, 465, 832, 862, 1029, 1060, 1132, 1181
BstENI CCTNNNNNAGG 2 cut(s) 9, 619
BstF5I GGATG 4 cut(s) 211, 1093, 1567, 1657
BstFNI CGCG 1 cut(s) 274
BstHHI GCGC 3 cut(s) 274, 699, 712
BstKTI GATC 9 cut(s) 70, 127, 346, 452, 489, 831, 1120, 1297, 1701
BstMAI GTCTC 3 cut(s) 1441, 1607, 1635
BstMBI GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 6 cut(s) 199, 238, 280, 1004, 1025, 1484
BstNI CCWGG 3 cut(s) 1212, 1499, 1559
BstNSI RCATGY 1 cut(s) 716
BstSCI CCNGG 4 cut(s) 310, 1210, 1497, 1557
BstSFI CTRYAG 4 cut(s) 434, 651, 996, 1485
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 637, 1292
BstX2I RGATCY 2 cut(s) 343, 828
BstYI RGATCY 2 cut(s) 343, 828
BstZI CGGCCG 1 cut(s) 275
BsuRI GGCC 4 cut(s) 133, 277, 315, 461
BtsCI GGATG 4 cut(s) 211, 1093, 1567, 1657
BtsI GCAGTG 2 cut(s) 712, 1005
BtsIMutI CAGTG 7 cut(s) 712, 790, 840, 876, 926, 1005, 1335
BveI ACCTGC 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 1239
CfoI GCGC 3 cut(s) 274, 699, 712
Cfr10I RCCGGY 2 cut(s) 106, 277
Cfr13I GGNCC 4 cut(s) 132, 313, 459, 641
Csp6I GTAC 5 cut(s) 1053, 1244, 1379, 1389, 1721
CviAII CATG 8 cut(s) 62, 151, 211, 338, 676, 713, 1269, 1514
CviQI GTAC 5 cut(s) 1053, 1244, 1379, 1389, 1721
DdeI CTNAG 8 cut(s) 290, 465, 832, 862, 1029, 1060, 1132, 1181
DpnI GATC 9 cut(s) 69, 126, 345, 451, 488, 830, 1119, 1296, 1700
DpnII GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
DraI TTTAAA 1 cut(s) 376
DraIII CACNNNGTG 1 cut(s) 65
DriI GACNNNNNGTC 1 cut(s) 1620
EaeI YGGCCR 1 cut(s) 275
EagI CGGCCG 1 cut(s) 275
Eam1105I GACNNNNNGTC 1 cut(s) 1620
EclXI CGGCCG 1 cut(s) 275
Eco130I CCWWGG 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 1441
Eco32I GATATC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 641
Eco52I CGGCCG 1 cut(s) 275
Eco57I CTGAAG 2 cut(s) 1052, 1748
EcoNI CCTNNNNNAGG 2 cut(s) 9, 619
EcoO109I RGGNCCY 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 3 cut(s) 1210, 1497, 1557
EcoRV GATATC 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 8 cut(s) 65, 154, 214, 341, 679, 716, 1272, 1517
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FatI CATG 8 cut(s) 61, 150, 210, 337, 675, 712, 1268, 1513
Fnu4HI GCNGC 3 cut(s) 275, 651, 1281
FokI GGATG 4 cut(s) 218, 1080, 1574, 1664
Fsp4HI GCNGC 3 cut(s) 275, 651, 1281
FspAI RTGCGCAY 1 cut(s) 711
FspBI CTAG 4 cut(s) 6, 11, 693, 1569
FspI TGCGCA 1 cut(s) 711
GlaI GCGC 3 cut(s) 273, 698, 711
GluI GCNGC 3 cut(s) 275, 651, 1281
GsuI CTGGAG 2 cut(s) 180, 705
HaeIII GGCC 4 cut(s) 133, 277, 315, 461
HapII CCGG 3 cut(s) 107, 278, 311
HhaI GCGC 3 cut(s) 274, 699, 712
Hin1II CATG 8 cut(s) 65, 154, 214, 341, 679, 716, 1272, 1517
Hin6I GCGC 3 cut(s) 272, 697, 710
HinP1I GCGC 3 cut(s) 272, 697, 710
HindIII AAGCTT 2 cut(s) 32, 1045
HinfI GANTC 9 cut(s) 56, 318, 469, 590, 866, 949, 1583, 1592, 1673
HpaII CCGG 3 cut(s) 107, 278, 311
HphI GGTGA 5 cut(s) 77, 134, 464, 527, 1477
Hpy166II GTNNAC 2 cut(s) 1379, 1391
Hpy188III TCNNGA 6 cut(s) 26, 197, 481, 558, 1124, 1316
Hpy8I GTNNAC 2 cut(s) 1379, 1391
HpyAV CCTTC 7 cut(s) 113, 148, 523, 621, 1073, 1327, 1521
HpyCH4III ACNGT 1 cut(s) 824
HpyCH4IV ACGT 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 6 cut(s) 199, 238, 280, 1004, 1025, 1484
HpyF3I CTNAG 8 cut(s) 290, 465, 832, 862, 1029, 1060, 1132, 1181
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 8 cut(s) 65, 154, 214, 341, 679, 716, 1272, 1517
HspAI GCGC 3 cut(s) 272, 697, 710
Kzo9I GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
LmnI GCTCC 2 cut(s) 630, 1572
Lsp1109I GCAGC 2 cut(s) 637, 1292
LweI GCATC 2 cut(s) 154, 1408
MaeI CTAG 4 cut(s) 6, 11, 693, 1569
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 5 cut(s) 53, 855, 946, 1465, 1553
MalI GATC 9 cut(s) 69, 126, 345, 451, 488, 830, 1119, 1296, 1700
MboI GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
MboII GAAGA 9 cut(s) 53, 353, 439, 496, 600, 603, 1112, 1682, 1741
MfeI CAATTG 2 cut(s) 242, 1145
MflI RGATCY 2 cut(s) 343, 828
MlyI GAGTC 3 cut(s) 50, 312, 943
MmeI TCCRAC 4 cut(s) 243, 310, 843, 1629
MroXI GAANNNNTTC 2 cut(s) 742, 1582
MslI CAYNNNNRTG 1 cut(s) 978
MspCI CTTAAG 2 cut(s) 619, 1048
MspI CCGG 3 cut(s) 107, 278, 311
MspR9I CCNGG 4 cut(s) 312, 1212, 1499, 1559
MunI CAATTG 2 cut(s) 242, 1145
Mva1269I GAATGC 2 cut(s) 1526, 1774
MvaI CCWGG 3 cut(s) 1212, 1499, 1559
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 6 cut(s) 199, 238, 280, 1004, 1025, 1484
NciI CCSGG 1 cut(s) 312
NdeII GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
NlaIII CATG 8 cut(s) 65, 154, 214, 341, 679, 716, 1272, 1517
NlaIV GGNNCC 3 cut(s) 282, 643, 1216
NmeAIII GCCGAG 1 cut(s) 1636
NmuCI GTSAC 3 cut(s) 53, 946, 1465
NsbI TGCGCA 1 cut(s) 711
NspI RCATGY 1 cut(s) 716
PaqCI CACCTGC 1 cut(s) 492
PctI GAATGC 2 cut(s) 1526, 1774
PdmI GAANNNNTTC 2 cut(s) 742, 1582
PfeI GAWTC 6 cut(s) 469, 590, 866, 1583, 1592, 1673
PkrI GCNGC 3 cut(s) 276, 652, 1282
PleI GAGTC 3 cut(s) 50, 312, 943
PpsI GAGTC 3 cut(s) 50, 312, 943
Psp6I CCWGG 3 cut(s) 1210, 1497, 1557
PspGI CCWGG 3 cut(s) 1210, 1497, 1557
PspN4I GGNNCC 3 cut(s) 282, 643, 1216
PspPI GGNCC 4 cut(s) 132, 313, 459, 641
PstI CTGCAG 3 cut(s) 655, 1000, 1489
PsuI RGATCY 2 cut(s) 343, 828
RsaI GTAC 5 cut(s) 1054, 1245, 1380, 1390, 1722
RsaNI GTAC 5 cut(s) 1053, 1244, 1379, 1389, 1721
RseI CAYNNNNRTG 1 cut(s) 978
SatI GCNGC 3 cut(s) 275, 651, 1281
Sau3AI GATC 9 cut(s) 67, 124, 343, 449, 486, 828, 1117, 1294, 1698
Sau96I GGNCC 4 cut(s) 132, 313, 459, 641
SchI GAGTC 3 cut(s) 50, 312, 943
ScrFI CCNGG 4 cut(s) 312, 1212, 1499, 1559
SfaNI GCATC 2 cut(s) 154, 1408
SfcI CTRYAG 4 cut(s) 434, 651, 996, 1485
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 3 cut(s) 26, 619, 1048
SmoI CTYRAG 3 cut(s) 26, 619, 1048
SsiI CCGC 2 cut(s) 274, 1178
SspI AATATT 2 cut(s) 916, 1776
SspMI CTAG 4 cut(s) 6, 11, 693, 1569
StyD4I CCNGG 4 cut(s) 310, 1210, 1497, 1557
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 1 cut(s) 824
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 448, 480, 557, 1249
TatI WGTACW 2 cut(s) 1052, 1243
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 6 cut(s) 469, 590, 866, 1583, 1592, 1673
TscAI CASTG 7 cut(s) 712, 790, 840, 876, 926, 1005, 1335
TseFI GTSAC 3 cut(s) 53, 946, 1465
TseI GCWGC 2 cut(s) 650, 1280
Tsp45I GTSAC 3 cut(s) 53, 946, 1465
TspDTI ATGAA 7 cut(s) 167, 354, 804, 1127, 1533, 1578, 1781
TspGWI ACGGA 2 cut(s) 1086, 1207
TspRI CASTG 7 cut(s) 712, 790, 840, 876, 926, 1005, 1335
Vha464I CTTAAG 2 cut(s) 619, 1048
VpaK11BI GGWCC 1 cut(s) 641
XagI CCTNNNNNAGG 2 cut(s) 9, 619
XapI RAATTY 4 cut(s) 443, 925, 1109, 1198
XceI RCATGY 1 cut(s) 716
XmaJI CCTAGG 1 cut(s) 10
XmnI GAANNNNTTC 2 cut(s) 742, 1582
XspI CTAG 4 cut(s) 6, 11, 693, 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.