FvH4_2g05220

PRKR-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012334
Physical Location & Seq
Forward (+)
4319564 .. 4321757
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05220.t1

Sequence Viewer

Length: 525 bp
ATGGCATCCGGAAATCCCAGAGACGGCGACTTGCAGATGGTGCCGCGGCGCGAGAGGAAGCTAATGCAACCGCCAAGGCCTCCATCACCACCGCCGCCGTCGACGGCGATCGTGGAGTACACGCCGCCGGTGTTCAAGGAGGAGGAAGAGGATCTCGAGATCAAGCTCCGGCGGATACTCGAGAACGTGCCCGTCCGCGTCAGCAACACTTCCGGTAGTTCCGCTGGTTCTGGCTCCGGTGACTTTCATCAGTATCGACAGATGAGACGGAAAGAGCAAGACAGGCTTGCTAGGATGGAGGTCGACTATCAGAAAAGGAAAGAAGAGGCAGAGTTCAACATGAGACGGGAGGAAAGATTGAAAGCAGCTGAAGAGCGGACAGCAAAGAAGCGTTTGAAACGTCAAAAGAAGAAGCAGAAGAGGAAGGAGAAGAGAATGAAAACAAATTCAGGCGGCGAAGAGAATAACCAGAAAGAAGAATCCTCTGACGATGACGAGAACTCTGATGGTAATGCTACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1168

Protein Analysis

175

Amino Acids

20.26

Weight (kDa)

9.49

Isoelectric Point (pI)

68.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1168 PF06658 57 - 171 2.5e-28 Protein of unknown function (DUF1168)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 40
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 2 cut(s) 101, 303
AccII CGCG 3 cut(s) 46, 51, 198
AccIII TCCGGA 1 cut(s) 8
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 4 cut(s) 136, 337, 361, 397
AluBI AGCT 3 cut(s) 61, 166, 368
AluI AGCT 3 cut(s) 61, 166, 368
Alw26I GTCTC 3 cut(s) 15, 259, 337
AlwI GGATC 1 cut(s) 159
Ama87I CYCGRG 2 cut(s) 155, 179
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 78, 251
AvaI CYCGRG 2 cut(s) 155, 179
BaeGI GKGCMC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 377
BccI CCATC 4 cut(s) 31, 91, 289, 500
BceAI ACGGC 3 cut(s) 40, 82, 120
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 15, 259, 337
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 516
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 6 cut(s) 44, 47, 95, 125, 366, 454
BlsI GCNGC 6 cut(s) 45, 48, 96, 126, 367, 455
BmeT110I CYCGRG 2 cut(s) 155, 179
BmiI GGNNCC 2 cut(s) 42, 235
BmsI GCATC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 44, 74
BsaWI WCCGGW 3 cut(s) 8, 212, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse118I RCCGGY 1 cut(s) 127
BseAI TCCGGA 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 44, 74
BseGI GGATG 2 cut(s) 5, 300
BseLI CCNNNNNNNGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 1 cut(s) 377
Bsh1236I CGCG 3 cut(s) 46, 51, 198
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 79
BshNI GGYRCC 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 111
BsiHKCI CYCGRG 2 cut(s) 155, 179
BsiSI CCGG 5 cut(s) 9, 128, 169, 213, 237
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 3 cut(s) 15, 259, 337
BsmBI CGTCTC 3 cut(s) 15, 259, 337
BsnI GGCC 1 cut(s) 79
BsoBI CYCGRG 2 cut(s) 155, 179
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 108, 151, 159
BspANI GGCC 1 cut(s) 79
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 3 cut(s) 46, 51, 198
BspLI GGNNCC 2 cut(s) 42, 235
BspPI GGATC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 44, 74
BssMI GATC 3 cut(s) 108, 151, 159
BssT1I CCWWGG 1 cut(s) 74
Bst6I CTCTTC 6 cut(s) 141, 318, 366, 413, 425, 453
BstAPI GCANNNNNTGC 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 2 cut(s) 5, 300
BstFNI CGCG 3 cut(s) 46, 51, 198
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 3 cut(s) 111, 154, 162
BstMAI GTCTC 3 cut(s) 15, 259, 337
BstMBI GATC 3 cut(s) 108, 151, 159
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 2 cut(s) 40, 283
BstSFI CTRYAG 1 cut(s) 516
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 3 cut(s) 46, 51, 198
BstV1I GCAGC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 1 cut(s) 79
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 2 cut(s) 5, 300
Cac8I GCNNGC 1 cut(s) 288
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 127
Cfr42I CCGCGG 1 cut(s) 47
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 340
CviJI RGCY 6 cut(s) 61, 79, 166, 234, 286, 368
CviKI_1 RGCY 6 cut(s) 61, 79, 166, 234, 286, 368
CviQI GTAC 1 cut(s) 118
DpnI GATC 3 cut(s) 110, 153, 161
DpnII GATC 3 cut(s) 108, 151, 159
Eam1104I CTCTTC 6 cut(s) 141, 318, 366, 413, 425, 453
EarI CTCTTC 6 cut(s) 141, 318, 366, 413, 425, 453
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 74
Eco147I AGGCCT 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 155, 179
EcoT14I CCWWGG 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 74
Esp3I CGTCTC 3 cut(s) 15, 259, 337
FaeI CATG 1 cut(s) 343
FaiI YATR 1 cut(s) 341
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 1 cut(s) 339
FblI GTMKAC 2 cut(s) 101, 303
Fnu4HI GCNGC 6 cut(s) 44, 47, 95, 125, 366, 454
FokI GGATG 1 cut(s) 307
Fsp4HI GCNGC 6 cut(s) 44, 47, 95, 125, 366, 454
FspBI CTAG 1 cut(s) 291
GlaI GCGC 1 cut(s) 50
GluI GCNGC 6 cut(s) 44, 47, 95, 125, 366, 454
HaeIII GGCC 1 cut(s) 79
HapII CCGG 5 cut(s) 9, 128, 169, 213, 237
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 51
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 2 cut(s) 102, 304
HindII GTYRAC 2 cut(s) 102, 304
HinfI GANTC 1 cut(s) 479
HpaII CCGG 5 cut(s) 9, 128, 169, 213, 237
HphI GGTGA 2 cut(s) 78, 251
Hpy166II GTNNAC 3 cut(s) 102, 120, 304
Hpy188I TCNGA 3 cut(s) 312, 487, 505
Hpy188III TCNNGA 4 cut(s) 9, 155, 157, 181
Hpy8I GTNNAC 3 cut(s) 102, 120, 304
Hpy99I CGWCG 2 cut(s) 103, 106
HpyAV CCTTC 1 cut(s) 418
HpyCH4IV ACGT 2 cut(s) 186, 400
HpyCH4V TGCA 2 cut(s) 34, 67
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 283
HpySE526I ACGT 2 cut(s) 186, 400
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 1 cut(s) 49
Kpn2I TCCGGA 1 cut(s) 8
KspI CCGCGG 1 cut(s) 47
Kzo9I GATC 3 cut(s) 108, 151, 159
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 171, 239
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 291
MaeII ACGT 2 cut(s) 186, 400
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 110, 153, 161
MbiI CCGCTC 1 cut(s) 376
MboI GATC 3 cut(s) 108, 151, 159
MboII GAAGA 8 cut(s) 158, 335, 383, 421, 430, 442, 470, 488
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 1 cut(s) 445
MroI TCCGGA 1 cut(s) 8
MspA1I CMGCKG 3 cut(s) 46, 224, 368
MspI CCGG 5 cut(s) 9, 128, 169, 213, 237
MvnI CGCG 3 cut(s) 46, 51, 198
MwoI GCNNNNNNNGC 2 cut(s) 40, 283
NdeII GATC 3 cut(s) 108, 151, 159
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 42, 235
NmuCI GTSAC 1 cut(s) 239
PaeR7I CTCGAG 2 cut(s) 155, 179
PceI AGGCCT 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 6 cut(s) 45, 48, 96, 126, 367, 455
Ple19I CGATCG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 42, 235
PsuI RGATCY 1 cut(s) 151
PvuI CGATCG 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SacII CCGCGG 1 cut(s) 47
SalI GTCGAC 2 cut(s) 100, 302
SapI GCTCTTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 44, 47, 95, 125, 366, 454
Sau3AI GATC 3 cut(s) 108, 151, 159
SduI GDGCHC 1 cut(s) 192
SetI ASST 6 cut(s) 63, 168, 189, 303, 370, 403
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 516
Sfr274I CTCGAG 2 cut(s) 155, 179
Sfr303I CCGCGG 1 cut(s) 47
SgrAI CRCCGGYG 1 cut(s) 127
SgrBI CCGCGG 1 cut(s) 47
SgrDI CGTCGACG 1 cut(s) 100
SlaI CTCGAG 2 cut(s) 155, 179
SmlI CTYRAG 2 cut(s) 155, 179
SmoI CTYRAG 2 cut(s) 155, 179
Sse9I AATT 1 cut(s) 445
SseBI AGGCCT 1 cut(s) 79
SspMI CTAG 1 cut(s) 291
StuI AGGCCT 1 cut(s) 79
StyI CCWWGG 1 cut(s) 74
TaiI ACGT 2 cut(s) 189, 403
TaqI TCGA 5 cut(s) 101, 156, 180, 256, 303
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 117
TauI GCSGC 5 cut(s) 46, 49, 97, 127, 456
TfiI GAWTC 1 cut(s) 479
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 236, 452
TspGWI ACGGA 1 cut(s) 283
XapI RAATTY 1 cut(s) 445
XhoI CTCGAG 2 cut(s) 155, 179
XmiI GTMKAC 2 cut(s) 101, 303
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.