MD05G1089500.v1.1

PRKR-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012334
Physical Location & Seq
Reverse (-)
18967390 .. 18969692
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1089500.v1.1.491

Sequence Viewer

Length: 558 bp
ATGTCGTCGGGAAGACCCAAAGACGGGGACATGCAACTAGTGGCGGCGCCGGAGAGGAAACTAGCCCAGCCGCCTGACAGGAAACTAATGCTACCGCCAAAACCACCGACGGCAGCCGGCTCGACGGCGCTTGTGGAGTATACGCCGCCGGTGTTCAAGGAGGAGGAAGAGGACCTCGAGATCAAGCTCCGGAGGATTCTCGAAAATGTACCGGTTCGCGTCAGTAACACCTCCGGTAGCTCCGCCGGTTCGGGCTCTGGCGACTTCCATCAGTATCGGCAAATGAGACGGAAAGAGCAAGACAGGATTGCTAGGATGGACGTGGACTATCAAAGAAGGAAAGAAGAGGCTGAGTTCAATATGAGAAGGGAGGAAAGACTGAGACAGGCTGAAGAAAAGACAGCAAAGAAGCGTTTGAAACGTCAAAAGAAAAAGCAGAGAAAAAATGAGAAGAAGATGAAAACAAATTCAGGTGGCGAAGAAAAACAGAAAGAAGAGCCCTCGGACGAAGAGGGGAACTCTGATGATCAAACTGAACAAGCTTGTACCAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1168

Protein Analysis

186

Amino Acids

21.39

Weight (kDa)

9.42

Isoelectric Point (pI)

71.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1168 PF06658 64 - 180 8.1e-27 Protein of unknown function (DUF1168)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 140
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 5 cut(s) 44, 71, 95, 146, 243
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 2 cut(s) 210, 547
AfiI CCNNNNNNNGG 2 cut(s) 23, 24
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 3 cut(s) 157, 358, 418
AhlI ACTAGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 322
AluBI AGCT 3 cut(s) 187, 240, 542
AluI AGCT 3 cut(s) 187, 240, 542
Alw26I GTCTC 2 cut(s) 280, 376
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 189
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 466
AsiGI ACCGGT 1 cut(s) 211
AspLEI GCGC 2 cut(s) 49, 130
AspS9I GGNCC 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 2 cut(s) 257, 501
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 125
BccI CCATC 2 cut(s) 276, 310
BceAI ACGGC 2 cut(s) 126, 141
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 2 cut(s) 280, 376
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 3 cut(s) 38, 62, 312
BfoI RGCGCY 2 cut(s) 50, 131
BisI GCNGC 4 cut(s) 45, 71, 114, 146
BlsI GCNGC 4 cut(s) 46, 72, 115, 147
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 3 cut(s) 189, 211, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 24
Bse118I RCCGGY 4 cut(s) 116, 148, 211, 245
BseAI TCCGGA 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 23, 24
BseMII CTCAG 2 cut(s) 342, 371
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 7 cut(s) 50, 117, 149, 190, 212, 234, 246
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 23, 24
BsmAI GTCTC 2 cut(s) 280, 376
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 257, 501
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 180, 526
BspACI CCGC 5 cut(s) 44, 71, 95, 146, 243
BspCNI CTCAG 2 cut(s) 343, 372
BspEI TCCGGA 1 cut(s) 189
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 48
BspQI GCTCTTC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 46
BsrFI RCCGGY 4 cut(s) 116, 148, 211, 245
BssAI RCCGGY 4 cut(s) 116, 148, 211, 245
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 2 cut(s) 180, 526
BssNAI GTATAC 1 cut(s) 141
BssNI GRCGYC 1 cut(s) 47
Bst1107I GTATAC 1 cut(s) 141
Bst6I CTCTTC 4 cut(s) 162, 339, 489, 504
BstACI GRCGYC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 351, 380
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 2 cut(s) 50, 131
BstHHI GCGC 2 cut(s) 49, 130
BstKTI GATC 2 cut(s) 183, 529
BstMAI GTCTC 2 cut(s) 280, 376
BstMBI GATC 2 cut(s) 180, 526
BstNSI RCATGY 1 cut(s) 34
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 125
BstV2I GAAGAC 1 cut(s) 19
BstZ17I GTATAC 1 cut(s) 141
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 2 cut(s) 49, 130
Cfr10I RCCGGY 4 cut(s) 116, 148, 211, 245
Cfr13I GGNCC 1 cut(s) 172
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 209, 546
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 1 cut(s) 31
CviQI GTAC 2 cut(s) 209, 546
DdeI CTNAG 2 cut(s) 351, 380
DinI GGCGCC 1 cut(s) 48
DpnI GATC 2 cut(s) 182, 528
DpnII GATC 2 cut(s) 180, 526
Eam1104I CTCTTC 4 cut(s) 162, 339, 489, 504
EarI CTCTTC 4 cut(s) 162, 339, 489, 504
EciI GGCGGA 1 cut(s) 232
Eco24I GRGCYC 2 cut(s) 257, 501
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 172
EcoT38I GRGCYC 2 cut(s) 257, 501
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 1 cut(s) 34
FaiI YATR 3 cut(s) 32, 141, 362
FaqI GGGAC 1 cut(s) 41
FatI CATG 1 cut(s) 30
FbaI TGATCA 1 cut(s) 526
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 4 cut(s) 45, 71, 114, 146
FokI GGATG 1 cut(s) 328
FriOI GRGCYC 2 cut(s) 257, 501
Fsp4HI GCNGC 4 cut(s) 45, 71, 114, 146
FspBI CTAG 3 cut(s) 38, 62, 312
GlaI GCGC 2 cut(s) 48, 129
GluI GCNGC 4 cut(s) 45, 71, 114, 146
GsaI CCCAGC 1 cut(s) 70
HaeII RGCGCY 2 cut(s) 50, 131
HapII CCGG 7 cut(s) 50, 117, 149, 190, 212, 234, 246
HgaI GACGC 1 cut(s) 208
HhaI GCGC 2 cut(s) 49, 130
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 2 cut(s) 47, 128
HinP1I GCGC 2 cut(s) 47, 128
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 1 cut(s) 196
HpaII CCGG 7 cut(s) 50, 117, 149, 190, 212, 234, 246
Hpy166II GTNNAC 2 cut(s) 141, 325
Hpy188I TCNGA 2 cut(s) 505, 523
Hpy188III TCNNGA 4 cut(s) 9, 178, 190, 200
Hpy8I GTNNAC 2 cut(s) 141, 325
Hpy99I CGWCG 3 cut(s) 10, 112, 127
HpyAV CCTTC 2 cut(s) 330, 360
HpyCH4IV ACGT 2 cut(s) 321, 421
HpyCH4V TGCA 1 cut(s) 34
HpyF3I CTNAG 2 cut(s) 351, 380
HpySE526I ACGT 2 cut(s) 321, 421
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 2 cut(s) 47, 128
KasI GGCGCC 1 cut(s) 46
Kpn2I TCCGGA 1 cut(s) 189
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 2 cut(s) 180, 526
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 2 cut(s) 192, 245
Lsp1109I GCAGC 1 cut(s) 125
MaeI CTAG 3 cut(s) 38, 62, 312
MaeII ACGT 2 cut(s) 321, 421
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 182, 528
MboI GATC 2 cut(s) 180, 526
MboII GAAGA 9 cut(s) 24, 179, 356, 404, 463, 466, 491, 506, 521
MhlI GDGCHC 2 cut(s) 257, 501
MluCI AATT 1 cut(s) 466
Mly113I GGCGCC 1 cut(s) 47
MroI TCCGGA 1 cut(s) 189
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 1 cut(s) 556
MspI CCGG 7 cut(s) 50, 117, 149, 190, 212, 234, 246
MvnI CGCG 1 cut(s) 219
NaeI GCCGGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 47
NdeII GATC 2 cut(s) 180, 526
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 489
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 196
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 4 cut(s) 46, 72, 115, 147
PluTI GGCGCC 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 172
PspPPI RGGWCCY 1 cut(s) 172
RsaI GTAC 2 cut(s) 210, 547
RsaNI GTAC 2 cut(s) 209, 546
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 1 cut(s) 556
SatI GCNGC 4 cut(s) 45, 71, 114, 146
Sau3AI GATC 2 cut(s) 180, 526
Sau96I GGNCC 1 cut(s) 172
SduI GDGCHC 2 cut(s) 257, 501
SetI ASST 8 cut(s) 177, 189, 233, 242, 324, 424, 475, 544
SfoI GGCGCC 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 176
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 1 cut(s) 466
SsiI CCGC 5 cut(s) 44, 71, 95, 146, 243
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 3 cut(s) 38, 62, 312
TaiI ACGT 2 cut(s) 324, 424
TaqI TCGA 3 cut(s) 122, 177, 201
TasI AATT 1 cut(s) 466
TauI GCSGC 3 cut(s) 47, 73, 148
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 556
Tru9I TTAA 1 cut(s) 556
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 1 cut(s) 466
XceI RCATGY 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 3 cut(s) 38, 62, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.