MD05G1088900.v1.1

PRKR-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012334
Physical Location & Seq
Reverse (-)
18940334 .. 18942124
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1088900.v1.1.491

Sequence Viewer

Length: 588 bp
ATGTCGTCGGGAAGACCCAAAGACGGGGACATGCAACTAGTGGCGGCGCCGGAGAGGAAACTAGCCCAGCCGCCTGACAGGAAACTAATGCTACCGCCAAAACCACCGACGGCAGCCGGCTCGACGGCGCTTGTGGAGTATACGCCGCCGGTGTTCAAGGAGGAGGAAGAGGACCTCGAGATCAAGCTCCGGAGGATTCTCGAAAATGTACCGGTTCGCGTCAGTAACACCTCCGGTAGCTCCGCCGGTTCGGGCTCTGGCGACTTCCATCAGAATGCAGTGTTGTCTCCTTTCAAGTTTAAACTTTGGGACAAGAGATGCCCGGAATTGTCTTGTTTGTCTTGCTGCAATAAAATAGCTTCTTTCTCAACAAAAACAAAGTGCCCGGATTTTCACGTACTGCAATACAATTATCGGCAAATGAGACGGAAAGAGCAAGACAGGATTGCTAGGATGGACGTGGACTATCAAAGAAGGAAAGAAGAGGCTGAGTTCAATATGAGAAGGGAGGAAAGACTGAGACAGGCTGAAGAAAAGACAGCAAAGAAGCGTTTTGAAACGTCAAAAGAAAAAGCAGAGAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1168

Protein Analysis

196

Amino Acids

22.51

Weight (kDa)

9.43

Isoelectric Point (pI)

62.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1168 PF06658 130 - 193 9e-09 Protein of unknown function (DUF1168)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 140
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 5 cut(s) 44, 71, 95, 146, 243
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 2 cut(s) 210, 399
AfiI CCNNNNNNNGG 2 cut(s) 23, 24
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 4 cut(s) 157, 295, 496, 557
AhlI ACTAGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 460
AluBI AGCT 3 cut(s) 187, 240, 359
AluI AGCT 3 cut(s) 187, 240, 359
Alw26I GTCTC 3 cut(s) 291, 418, 514
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 113, 345
AsiGI ACCGGT 1 cut(s) 211
AspLEI GCGC 2 cut(s) 49, 130
AspS9I GGNCC 1 cut(s) 172
AsuC2I CCSGG 2 cut(s) 323, 386
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 125, 332
BccI CCATC 2 cut(s) 276, 448
BceAI ACGGC 2 cut(s) 126, 141
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BcnI CCSGG 2 cut(s) 323, 386
BcoDI GTCTC 3 cut(s) 291, 418, 514
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 3 cut(s) 38, 62, 450
BfoI RGCGCY 2 cut(s) 50, 131
BisI GCNGC 5 cut(s) 45, 71, 114, 146, 346
BlsI GCNGC 5 cut(s) 46, 72, 115, 147, 347
Bme1390I CCNGG 2 cut(s) 323, 386
Bme18I GGWCC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 323, 386
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 19
BpuMI CCSGG 2 cut(s) 323, 386
BsaAI YACGTR 1 cut(s) 397
BsaHI GRCGYC 1 cut(s) 47
BsaWI WCCGGW 3 cut(s) 189, 211, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 24
Bse118I RCCGGY 4 cut(s) 116, 148, 211, 245
BseAI TCCGGA 1 cut(s) 189
BseGI GGATG 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 23, 24
BseMII CTCAG 2 cut(s) 480, 509
BseRI GAGGAG 1 cut(s) 176
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 2 cut(s) 125, 332
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 9 cut(s) 50, 117, 149, 190, 212, 234, 246, 323, 386
BslFI GGGAC 2 cut(s) 41, 323
BslI CCNNNNNNNGG 2 cut(s) 23, 24
BsmAI GTCTC 3 cut(s) 291, 418, 514
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 41, 323
BsmI GAATGC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 257, 386
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 5 cut(s) 44, 71, 95, 146, 243
BspCNI CTCAG 2 cut(s) 481, 510
BspEI TCCGGA 1 cut(s) 189
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 46
BsrFI RCCGGY 4 cut(s) 116, 148, 211, 245
BssAI RCCGGY 4 cut(s) 116, 148, 211, 245
BssMI GATC 1 cut(s) 180
BssNAI GTATAC 1 cut(s) 141
BssNI GRCGYC 1 cut(s) 47
Bst1107I GTATAC 1 cut(s) 141
Bst6I CTCTTC 2 cut(s) 162, 477
BstACI GRCGYC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 489, 518
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 2 cut(s) 50, 131
BstHHI GCGC 2 cut(s) 49, 130
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 3 cut(s) 291, 418, 514
BstMBI GATC 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 321, 384
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 125, 332
BstV2I GAAGAC 1 cut(s) 19
BstZ17I GTATAC 1 cut(s) 141
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 2 cut(s) 49, 130
Cfr10I RCCGGY 4 cut(s) 116, 148, 211, 245
Cfr13I GGNCC 1 cut(s) 172
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 209, 398
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 1 cut(s) 31
CviQI GTAC 2 cut(s) 209, 398
DdeI CTNAG 2 cut(s) 489, 518
DinI GGCGCC 1 cut(s) 48
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 162, 477
EarI CTCTTC 2 cut(s) 162, 477
EciI GGCGGA 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 257
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 418
FaeI CATG 1 cut(s) 34
FaiI YATR 3 cut(s) 32, 141, 500
FaqI GGGAC 2 cut(s) 41, 323
FatI CATG 1 cut(s) 30
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 5 cut(s) 45, 71, 114, 146, 346
FokI GGATG 1 cut(s) 466
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 5 cut(s) 45, 71, 114, 146, 346
FspBI CTAG 3 cut(s) 38, 62, 450
GlaI GCGC 2 cut(s) 48, 129
GluI GCNGC 5 cut(s) 45, 71, 114, 146, 346
GsaI CCCAGC 1 cut(s) 70
HaeII RGCGCY 2 cut(s) 50, 131
HapII CCGG 9 cut(s) 50, 117, 149, 190, 212, 234, 246, 323, 386
HgaI GACGC 1 cut(s) 208
HhaI GCGC 2 cut(s) 49, 130
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 2 cut(s) 47, 128
HinP1I GCGC 2 cut(s) 47, 128
HinfI GANTC 1 cut(s) 196
HpaII CCGG 9 cut(s) 50, 117, 149, 190, 212, 234, 246, 323, 386
Hpy166II GTNNAC 2 cut(s) 141, 463
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 4 cut(s) 9, 178, 190, 200
Hpy8I GTNNAC 2 cut(s) 141, 463
Hpy99I CGWCG 3 cut(s) 10, 112, 127
HpyAV CCTTC 2 cut(s) 468, 498
HpyCH4IV ACGT 3 cut(s) 396, 459, 560
HpyCH4V TGCA 4 cut(s) 34, 278, 348, 403
HpyF3I CTNAG 2 cut(s) 489, 518
HpySE526I ACGT 3 cut(s) 396, 459, 560
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 2 cut(s) 47, 128
KasI GGCGCC 1 cut(s) 46
Kpn2I TCCGGA 1 cut(s) 189
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 192, 245
Lsp1109I GCAGC 2 cut(s) 125, 332
LweI GCATC 1 cut(s) 308
MaeI CTAG 3 cut(s) 38, 62, 450
MaeII ACGT 3 cut(s) 396, 459, 560
MaeIII GTNAC 1 cut(s) 224
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 4 cut(s) 24, 179, 494, 542
MhlI GDGCHC 2 cut(s) 257, 386
MluCI AATT 2 cut(s) 326, 409
Mly113I GGCGCC 1 cut(s) 47
MnlI CCTC 9 cut(s) 48, 154, 157, 163, 185, 186, 241, 478, 502
MroI TCCGGA 1 cut(s) 189
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 9 cut(s) 50, 117, 149, 190, 212, 234, 246, 323, 386
MspR9I CCNGG 2 cut(s) 323, 386
MssI GTTTAAAC 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 280
MvnI CGCG 1 cut(s) 219
NaeI GCCGGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 47
NciI CCSGG 2 cut(s) 323, 386
NdeII GATC 1 cut(s) 180
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 176
PctI GAATGC 1 cut(s) 280
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 196
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 5 cut(s) 46, 72, 115, 147, 347
PluTI GGCGCC 1 cut(s) 50
PmeI GTTTAAAC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 397
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 172
PspPPI RGGWCCY 1 cut(s) 172
RsaI GTAC 2 cut(s) 210, 399
RsaNI GTAC 2 cut(s) 209, 398
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 5 cut(s) 45, 71, 114, 146, 346
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 323, 386
SduI GDGCHC 2 cut(s) 257, 386
SetI ASST 8 cut(s) 177, 189, 233, 242, 361, 399, 462, 563
SfaNI GCATC 1 cut(s) 308
SfoI GGCGCC 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 176
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 172
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 2 cut(s) 326, 409
SsiI CCGC 5 cut(s) 44, 71, 95, 146, 243
SspDI GGCGCC 1 cut(s) 46
SspMI CTAG 3 cut(s) 38, 62, 450
StyD4I CCNGG 2 cut(s) 321, 384
TaiI ACGT 3 cut(s) 399, 462, 563
TaqI TCGA 3 cut(s) 122, 177, 201
TasI AATT 2 cut(s) 326, 409
TauI GCSGC 3 cut(s) 47, 73, 148
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 285
TseI GCWGC 2 cut(s) 113, 345
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 172
XceI RCATGY 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 3 cut(s) 38, 62, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.