RchiOBHm_Chr6g0255351

PRKR-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012334
Physical Location & Seq
Forward (+)
10692362 .. 10694799
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22902

Sequence Viewer

Length: 525 bp
ATGTCGTCCGGAAAAGCCAAAGACGGGGACTTGCAGCTGGTGGCGCGGCCCGAGAGGAAGCTAATGCAACCGCCAAAGCCTCCAACACCACCGCCGGCGTCGACGGCGCTCGTGGAGTACACGCCGCCGGTGTTCAAGGAGGAGGAAGAGGATCTTGAGATCAAGCTCCGGCGGATTCTCGAAAACGTGCCCGTCCGAGTCAGCAACACTTCCGGTAGTTCCGCCGGTTCCGGCTCCGGTGACTTTCATCAGTATCGGCAGATGAGACGGAAAGAACAAGACAGGCTTGCTAGAATGGAGGTTGACTATCAGAAAAGGAAAGAGGAGGCAGAGTTCAATATGAGACGGGAGGAAAGATTGAAAGCAGCCGAAGAGCGGACAGCAAAGAAGCGTTTAAAACGTCAAAAGAAGAAGCAGAAAAAGAAAGAGAAGAGAATGAAAACAAATTCAGGTGGTGAAGAGAATAATCAGAAAGAAGAATCCTCTGACGATGACAAGAACTCTGATGGTGATGCTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1168

Protein Analysis

174

Amino Acids

20.05

Weight (kDa)

9.47

Isoelectric Point (pI)

63.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1168 PF06658 57 - 172 4.8e-28 Protein of unknown function (DUF1168)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 101
AccII CGCG 1 cut(s) 46
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 7 cut(s) 46, 71, 92, 125, 172, 222, 376
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 24, 375
AgsI TTSAA 3 cut(s) 136, 337, 361
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 37, 61, 166
AluI AGCT 3 cut(s) 37, 61, 166
Alw26I GTCTC 2 cut(s) 259, 337
AlwI GGATC 1 cut(s) 159
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 8
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 34, 365
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 2 cut(s) 46, 109
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 251, 467, 521
AvaI CYCGRG 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 192
BauI CACGAG 1 cut(s) 110
BbvI GCAGC 2 cut(s) 46, 377
BccI CCATC 1 cut(s) 500
BceAI ACGGC 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 259, 337
BfaI CTAG 1 cut(s) 291
BfoI RGCGCY 1 cut(s) 110
BisI GCNGC 4 cut(s) 35, 47, 125, 366
BlsI GCNGC 4 cut(s) 36, 48, 126, 367
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 229, 235
BmsI GCATC 1 cut(s) 502
BpuEI CTTGAG 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 98
BsaWI WCCGGW 3 cut(s) 8, 212, 236
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 375
Bse118I RCCGGY 3 cut(s) 94, 127, 224
BseAI TCCGGA 1 cut(s) 8
BseLI CCNNNNNNNGG 2 cut(s) 24, 375
BseRI GAGGAG 2 cut(s) 155, 338
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 2 cut(s) 46, 377
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 8 cut(s) 9, 95, 128, 169, 213, 225, 231, 237
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 24, 375
BsmAI GTCTC 2 cut(s) 259, 337
BsmBI CGTCTC 2 cut(s) 259, 337
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 1 cut(s) 49
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 2 cut(s) 151, 159
BspACI CCGC 7 cut(s) 46, 71, 92, 125, 172, 222, 376
BspANI GGCC 1 cut(s) 49
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 229, 235
BspPI GGATC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 366
BsrBI CCGCTC 1 cut(s) 376
BsrFI RCCGGY 3 cut(s) 94, 127, 224
BssAI RCCGGY 3 cut(s) 94, 127, 224
BssMI GATC 2 cut(s) 151, 159
BssNI GRCGYC 1 cut(s) 98
BssSI CACGAG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 110
Bst6I CTCTTC 4 cut(s) 141, 366, 425, 453
BstACI GRCGYC 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 96, 288
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 2 cut(s) 46, 109
BstKTI GATC 2 cut(s) 154, 162
BstMAI GTCTC 2 cut(s) 259, 337
BstMBI GATC 2 cut(s) 151, 159
BstMWI GCNNNNNNNGC 2 cut(s) 43, 104
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 46, 377
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 96, 288
CfoI GCGC 2 cut(s) 46, 109
Cfr10I RCCGGY 3 cut(s) 94, 127, 224
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 87
Csp6I GTAC 1 cut(s) 118
CviJI RGCY 9 cut(s) 17, 37, 49, 61, 79, 166, 234, 286, 368
CviKI_1 RGCY 9 cut(s) 17, 37, 49, 61, 79, 166, 234, 286, 368
CviQI GTAC 1 cut(s) 118
DpnI GATC 2 cut(s) 153, 161
DpnII GATC 2 cut(s) 151, 159
DraI TTTAAA 1 cut(s) 396
Eam1104I CTCTTC 4 cut(s) 141, 366, 425, 453
EarI CTCTTC 4 cut(s) 141, 366, 425, 453
EciI GGCGGA 2 cut(s) 187, 211
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 50
Esp3I CGTCTC 2 cut(s) 259, 337
FaiI YATR 1 cut(s) 341
FalI AAGNNNNNCTT 4 cut(s) 138, 170, 270, 302
FaqI GGGAC 1 cut(s) 41
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 4 cut(s) 35, 47, 125, 366
Fsp4HI GCNGC 4 cut(s) 35, 47, 125, 366
FspBI CTAG 1 cut(s) 291
GlaI GCGC 2 cut(s) 45, 108
GluI GCNGC 4 cut(s) 35, 47, 125, 366
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 1 cut(s) 49
HapII CCGG 8 cut(s) 9, 95, 128, 169, 213, 225, 231, 237
HgaI GACGC 1 cut(s) 87
HhaI GCGC 2 cut(s) 46, 109
Hin1I GRCGYC 1 cut(s) 98
Hin6I GCGC 2 cut(s) 44, 107
HinP1I GCGC 2 cut(s) 44, 107
HincII GTYRAC 2 cut(s) 102, 304
HindII GTYRAC 2 cut(s) 102, 304
HinfI GANTC 3 cut(s) 175, 198, 479
HpaII CCGG 8 cut(s) 9, 95, 128, 169, 213, 225, 231, 237
HphI GGTGA 3 cut(s) 251, 467, 521
Hpy166II GTNNAC 3 cut(s) 102, 120, 304
Hpy188I TCNGA 6 cut(s) 197, 312, 471, 487, 505, 520
Hpy188III TCNNGA 3 cut(s) 9, 155, 179
Hpy8I GTNNAC 3 cut(s) 102, 120, 304
Hpy99I CGWCG 2 cut(s) 103, 106
HpyCH4IV ACGT 2 cut(s) 186, 400
HpyCH4V TGCA 2 cut(s) 34, 67
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 104
HpySE526I ACGT 2 cut(s) 186, 400
Hsp92I GRCGYC 1 cut(s) 98
HspAI GCGC 2 cut(s) 44, 107
Kpn2I TCCGGA 1 cut(s) 8
KroI GCCGGC 1 cut(s) 94
KroNI GCCGGC 1 cut(s) 96
Kzo9I GATC 2 cut(s) 151, 159
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 171, 239
Lsp1109I GCAGC 2 cut(s) 46, 377
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 291
MaeII ACGT 2 cut(s) 186, 400
MaeIII GTNAC 1 cut(s) 239
MalI GATC 2 cut(s) 153, 161
MbiI CCGCTC 1 cut(s) 376
MboI GATC 2 cut(s) 151, 159
MboII GAAGA 6 cut(s) 158, 383, 421, 442, 470, 488
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 1 cut(s) 445
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 107
MreI CGCCGGCG 1 cut(s) 94
MroI TCCGGA 1 cut(s) 8
MroNI GCCGGC 1 cut(s) 94
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 8 cut(s) 9, 95, 128, 169, 213, 225, 231, 237
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 43, 104
NaeI GCCGGC 1 cut(s) 96
NdeII GATC 2 cut(s) 151, 159
NgoMIV GCCGGC 1 cut(s) 94
NlaIV GGNNCC 2 cut(s) 229, 235
NmuCI GTSAC 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 366
PdiI GCCGGC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 175, 479
PkrI GCNGC 4 cut(s) 36, 48, 126, 367
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 229, 235
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SalI GTCGAC 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 4 cut(s) 35, 47, 125, 366
Sau3AI GATC 2 cut(s) 151, 159
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 207
SduI GDGCHC 1 cut(s) 192
SetI ASST 7 cut(s) 39, 63, 168, 189, 303, 403, 454
SfaNI GCATC 1 cut(s) 502
SgrAI CRCCGGYG 2 cut(s) 94, 127
SgrDI CGTCGACG 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 1 cut(s) 445
SsiI CCGC 7 cut(s) 46, 71, 92, 125, 172, 222, 376
SspMI CTAG 1 cut(s) 291
TaiI ACGT 2 cut(s) 189, 403
TaqI TCGA 2 cut(s) 101, 180
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 117
TauI GCSGC 2 cut(s) 49, 127
TfiI GAWTC 2 cut(s) 175, 479
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 2 cut(s) 34, 365
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 2 cut(s) 236, 452
TspGWI ACGGA 1 cut(s) 283
XapI RAATTY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 101
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.