FvH4_2g13450
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014921
Physical Location & Seq
Forward (+)
11749147 .. 11753398
4252 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13450.t1

Sequence Viewer

Length: 3354 bp
ATGCATGTTGTCTCCTATACTTTCACCACCACCACTATAATCACCAACCTCAAGAACCCATTTCTTAACCATCTCCATCAAGCCCCTTTCTCAGCGACTACCCAAATGGAAAACCACAGACTCACCTGTACTAATCCTTCTTCTCTCTTTTCCTTCACATTGTTCATCATTTTCATGACAATGACCAAACTAACCTTGGTCTCCTCCCTGTCCCCTGATGGCCAAGCTCTTCTCTCCCTTCTTCCTGCAAAGCAATCTCCCTCTGTACTCTCCTCATGGAACCCATCAAGCCAGACACCATGTTCATGGCAAGGAATCACATGCTCGCCGCAAAACAGAGTTATCTCCCTCTCTCTCCCCAACATTTTCCTCAACCTCTCCTCTTTACCCTCAGAGCTCTCTTCTCTTTCATCTCTCCAGCTTCTCAATCTCTCCTCCACTAACATTTCCGGCACAATCCCACCTTCCTTTGGACAACTTAGTAATCTTCGCCTCCTCGACCTCTCCTCCAACTCTCTGTTTGGCCCCATTCCTTTAGAACTAGGCGCTCTCTCTGCTCTCCAGTTCCTCTTCTTGAACTCGAACAGATTGTCAGATAAAATCCCTCAACAACTTGCCAACCTCACTTCACTTCAAGCCCTTTGTCTCCAAGACAACCTCATCAACGGCTCGATACCCTCCCAACTCGGTTCTTTGGTTTCTCTCCAACAGTTTCGTATTGGTGGGAATCCATACATAACCGGAGAAATTCCTTCCCAGTTAGGACTGCTCACCAATCTCACCACATTCGGAGCGGCGGCTACTGGACTCTCCGGCACTATCCCACCCACATTTGGAAACTTGATCAATCTTCAGACACTAGCACTCTATGATACAGAAATAGTTGGTTCAATACCACCCGAAATCGGCCTCTGTTTTGAGCTTAGAAACTTGTACCTGCACATGAACAAGCTCACTGGCTCCATTCCTCCACAATTAGGTAAGCTGCAAAAGCTCACCAGCCTACTTGTTTGGGGAAATGCCCTATCAGGACCTATCCCTGATGAGATTTCCAATTGTTCATCACTTGTTGTTCTCGATGCTTCTGCAAATGATCTCTCTGGAGAGATTCCAAGAGACTTGGGGAAGTTGGTGGTCCTTGAACAGCTTCATCTATCGGATAACTCGCTTACAGGGTCGATTCCATCGCAGCTCAGTAACTGTAGTAGCCTAACAGCTCTTCAGCTTGACAAGAATCAGTTATCAGGGACAATTCCGTGGCAGGTTGGTAATTTGAAATACTTGCAGAGTTTCTTCTTGTGGGGAAATTCAGTGTCTGGAACTATTCCTGCCTCGTTTGGGAACTGCACTGAACTCTATGCACTTGATCTTTCGAGAAACAAGCTCACCGGGTCAATCCCTGAAGAGATTTTTGGTTTGAAGAAGCTGAGCAAGCTTTTGCTTCTGGGAAACTCTTTATCCGGTAGGTTGCCACCTAGTGTAGCAAAATGTCAATCACTAGTTCGACTGAGACTCGGTGAGAACCAGCTTTCAGGACAAATTCCAAAGGAGATAGGCCAACTGCAAAACCTTGTGTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGCCTTCCCATTGAGATTGCCAACATCACCGTTCTAGAGCTACTGGATGTGCACAATAACTATATTGGTGGCGAAATCCCATCTCAGCTTGGGGAGCTTGTTAATTTGGAGCAGCTTGATCTAAGCCGAAACAGCTTCAAGGGTGAGATTCCATGGAGCTTTGGGAACTTGAGTTACTTGAACAAGCTCATCATCAACAACAATCTTCTCACAGGTTCAATACCAAAGTCCTTTCGGAATTTACAGAAGCTGACATTGTTGGATTTGAGCTTCAACAGCCTCTCTGGTGCAATCCCATCTGAAATTGGTCATGTGACAAGCTTGCAAATCAGTTTGGACTTGAGTTCCAACAGTTTTACAGGAGAACTCCCGGAGACAATGGCGGGTTTAACACAATTACAGTGTCTTGATCTTTCCCATAATATGCTCTTTGGGAAAATTAAGGTTCTTGGTTCTCTAACTAGTCTGGCTTCCCTTAATATCTCCTGCAACAATTTCTCAGGTGCTATCCCGATAACAACCCCATTCTTCAGAGCTCTTTCATCAAATTCTTATCTTCAGAATCCACATCTTTGTGAGTCGAGTGATGGGTCTACTTGCTCTTCAAGTCTAATGCGGCAAAATGGTTTGAAATCTACGAAAACTATTGCTTTAATATCTGTGATTCTCTCTTCAGTGACCATAGCAGTTGTTGCTTCATGGATTCTCGTTATGCGAAATCATAGATATATGGTGGAGAAATCTTCAAGAACATTAGCATCCTCATCGGGGGCAGAAGATTTCTCTTATCCGTGGACTTTCATCCCATTCCAGAAGTTCAATTTCACTATTGACAACATCTTGGATTGTTTGAAAGAAGAAAATGTGATTGGAAAAGGATGCTCTGGGGTCGTCTACAAGGCAGAAATGCAAACTGGGGAGCTAATTGCGGTGAAGAAGTTGTGGAAAACAAAGGGAGAGGAAGAAGCAATAGACTCTTTCGCTGCAGAGATTCAAATTCTTGGACACATTCGCCACCGGAACATTGTAAAGCTCCTAGGGTACTGTTCAAATAGAAGTGTCAAGCTACTTCTTTACAACTTCATTCCCAATGGCAATCTACAACAACTACTGCAAGAAAATAGGAACTTGGACTGGGAAACAAGGTACAAGATTGCTATTGGGTCAGCTCAGGGTTTGGCATACCTTCATCATGACTGTGTCCCAGCAATTCTTCACAGGGATGTGAAGTGCAATAACATATTACTAGATTCCAAGTGTGAAGCTTATCTCGCAGATTTTGGTCTTGCGAAATTGATGAACTCCCCAACTTACCATCATGCTATGTCCAGAGTTGCTGGCTCTTATGGTTACATTGCACCAGAGTATGGATACACAATGAACATAACAGAGAAGAGTGATGTCTACAGCTATGGTGTAGTTTTGCTAGAGATCCTAAGCGGACGTAATGCCGTTCAGCCCCAAATTGGTGATGGACTACACATTGTAGAATGGGTGAAGAAGAAGATGGCAAGTTTTGAGCCAGCTTTATCAATACTAGATACAAAGCTGCAAGGCCTGCCAGATCAAATGGTTCAAGAGATGCTGCAAACACTCGGAATAGCAATGTTCTGTGTCAACTCTTCGCCGGCAGAACGACCCACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAACCTGAAGAGTGGGGAAAAACTTCCCAACCCCTAATAAAACAGTCTTCAAATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1118

Amino Acids

121.92

Weight (kDa)

6.39

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 71 - 109 5.7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 160 - 263 2.4e-08 Leucine-rich repeat region
LRR_14 PF23598 278 - 441 2.1e-10 Leucine-rich repeat region
LRR_8 PF13855 428 - 486 1.1e-07 Leucine rich repeat
LRR_14 PF23598 447 - 530 9.3e-06 Leucine-rich repeat region
LRR_14 PF23598 503 - 576 4.9e-06 Leucine-rich repeat region
LRR_14 PF23598 542 - 625 1.5e-06 Leucine-rich repeat region
LRR_14 PF23598 632 - 699 3.7e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 821 - 1091 5.4e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 822 - 1089 7.8e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 945, 1252
AccB7I CCANNNNNTGG 1 cut(s) 2975
AccBSI CCGCTC 1 cut(s) 794
AccI GTMKAC 3 cut(s) 2201, 2502, 3012
AciI CCGC 7 cut(s) 329, 794, 797, 1991, 2224, 2537, 3048
AclWI GGATC 1 cut(s) 3034
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 6 cut(s) 747, 1306, 1539, 1846, 2155, 2604
AcuI CTGAAG 7 cut(s) 836, 1205, 1422, 2122, 2150, 2265, 3318
AdeI CACNNNGTG 1 cut(s) 1478
AfaI GTAC 6 cut(s) 130, 267, 935, 1589, 2651, 2756
AfiI CCNNNNNNNGG 8 cut(s) 470, 833, 905, 977, 1338, 2376, 2975, 3074
AhlI ACTAGT 2 cut(s) 1498, 2069
AjuI GAANNNNNNNTTGG 4 cut(s) 1395, 1427, 2460, 2492
Alw21I GWGCWC 3 cut(s) 399, 1662, 2146
Alw26I GTCTC 6 cut(s) 16, 205, 650, 1110, 1504, 1976
Alw44I GTGCAC 1 cut(s) 1658
AlwI GGATC 1 cut(s) 3034
AlwNI CAGNNNCTG 3 cut(s) 1200, 1316, 1858
AoxI GGCC 6 cut(s) 220, 523, 907, 1555, 1609, 3163
ApaLI GTGCAC 1 cut(s) 1658
ApeKI GCWGC 6 cut(s) 985, 1189, 1720, 2591, 3157, 3193
ApoI RAATTY 6 cut(s) 747, 1306, 1539, 1846, 2155, 2604
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
Asp700I GAANNNNTTC 3 cut(s) 1146, 1598, 3313
AspA2I CCTAGG 1 cut(s) 2644
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 3 cut(s) 524, 1031, 1135
AsuC2I CCSGG 2 cut(s) 1390, 1979
AvaII GGWCC 2 cut(s) 1031, 1135
AvrII CCTAGG 1 cut(s) 2644
BaeGI GKGCMC 1 cut(s) 1662
BalI TGGCCA 1 cut(s) 222
BanII GRGCYC 2 cut(s) 399, 2146
BarI GAAGNNNNNNTAC 2 cut(s) 121, 153
BbsI GAAGAC 1 cut(s) 3330
Bbv12I GWGCWC 3 cut(s) 399, 1662, 2146
BbvI GCAGC 6 cut(s) 972, 1201, 1732, 2578, 3144, 3180
BceAI ACGGC 2 cut(s) 682, 3044
BcgI CGANNNNNNTGC 4 cut(s) 1067, 1101, 2355, 2389
BciVI GTATCC 1 cut(s) 2972
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 2 cut(s) 1390, 1979
BcoDI GTCTC 6 cut(s) 16, 205, 650, 1110, 1504, 1976
BcuI ACTAGT 2 cut(s) 1498, 2069
BfmI CTRYAG 3 cut(s) 1201, 2592, 3013
BfoI RGCGCY 1 cut(s) 549
BfuAI ACCTGC 2 cut(s) 945, 1252
BfuI GTATCC 1 cut(s) 2972
BlnI CCTAGG 1 cut(s) 2644
BlpI GCTNAGC 1 cut(s) 1427
Bme1390I CCNGG 2 cut(s) 1390, 1979
Bme18I GGWCC 2 cut(s) 1031, 1135
BmgT120I GGNCC 3 cut(s) 524, 1031, 1135
BmiI GGNNCC 3 cut(s) 281, 526, 961
BmrFI CCNGG 2 cut(s) 1390, 1979
BmrI ACTGGG 3 cut(s) 751, 2532, 2752
BmsI GCATC 4 cut(s) 1069, 2375, 2477, 3180
BmuI ACTGGG 3 cut(s) 751, 2532, 2752
BpiI GAAGAC 1 cut(s) 3330
BpmI CTGGAG 4 cut(s) 401, 545, 1122, 1626
Bpu10I CCTNAGC 2 cut(s) 2778, 3044
Bpu1102I GCTNAGC 1 cut(s) 1427
BpuEI CTTGAG 3 cut(s) 35, 1798, 1969
BpuMI CCSGG 2 cut(s) 1390, 1979
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 5 cut(s) 195, 1256, 1760, 2399, 2644
BsaWI WCCGGW 3 cut(s) 740, 1460, 2625
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 8 cut(s) 470, 833, 905, 977, 1338, 2376, 2975, 3074
Bse118I RCCGGY 1 cut(s) 3235
Bse1I ACTGG 7 cut(s) 562, 757, 808, 961, 1656, 2527, 2747
Bse3DI GCAATG 3 cut(s) 2961, 3219, 3269
BseDI CCNNGG 5 cut(s) 195, 1256, 1760, 2399, 2644
BseGI GGATG 5 cut(s) 1660, 2366, 2409, 2492, 2836
BseLI CCNNNNNNNGG 8 cut(s) 470, 833, 905, 977, 1338, 2376, 2975, 3074
BseMI GCAATG 3 cut(s) 2961, 3219, 3269
BseMII CTCAG 8 cut(s) 105, 405, 1207, 1418, 1499, 1706, 2121, 2792
BseNI ACTGG 7 cut(s) 562, 757, 808, 961, 1656, 2527, 2747
BseRI GAGGAG 6 cut(s) 193, 262, 370, 424, 485, 496
BseSI GKGCMC 1 cut(s) 1662
BseXI GCAGC 6 cut(s) 972, 1201, 1732, 2578, 3144, 3180
BseYI CCCAGC 1 cut(s) 2812
BsgI GTGCAG 2 cut(s) 923, 1330
BshFI GGCC 6 cut(s) 222, 525, 909, 1557, 1611, 3165
BsiHKAI GWGCWC 3 cut(s) 399, 1662, 2146
BsiSI CCGG 8 cut(s) 450, 741, 813, 1389, 1461, 1979, 2626, 3236
BslFI GGGAC 3 cut(s) 196, 1261, 2795
BslI CCNNNNNNNGG 8 cut(s) 470, 833, 905, 977, 1338, 2376, 2975, 3074
BsmAI GTCTC 6 cut(s) 16, 205, 650, 1110, 1504, 1976
BsmFI GGGAC 3 cut(s) 196, 1261, 2795
BsnI GGCC 6 cut(s) 222, 525, 909, 1557, 1611, 3165
Bso31I GGTCTC 1 cut(s) 205
Bsp1286I GDGCHC 3 cut(s) 399, 1662, 2146
Bsp1407I TGTACA 1 cut(s) 1587
Bsp143I GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
Bsp1720I GCTNAGC 1 cut(s) 1427
Bsp19I CCATGG 1 cut(s) 1760
BspACI CCGC 7 cut(s) 329, 794, 797, 1991, 2224, 2537, 3048
BspANI GGCC 6 cut(s) 222, 525, 909, 1557, 1611, 3165
BspCNI CTCAG 8 cut(s) 104, 404, 1206, 1419, 1500, 1705, 2120, 2791
BspHI TCATGA 2 cut(s) 174, 2800
BspLI GGNNCC 3 cut(s) 281, 526, 961
BspMAI CTGCAG 1 cut(s) 2596
BspMI ACCTGC 2 cut(s) 945, 1252
BspPI GGATC 1 cut(s) 3034
BspQI GCTCTTC 3 cut(s) 234, 1224, 2215
BspTNI GGTCTC 1 cut(s) 205
BsrBI CCGCTC 1 cut(s) 794
BsrDI GCAATG 3 cut(s) 2961, 3219, 3269
BsrFI RCCGGY 1 cut(s) 3235
BsrGI TGTACA 1 cut(s) 1587
BsrI ACTGG 7 cut(s) 562, 757, 808, 961, 1656, 2527, 2747
BssAI RCCGGY 1 cut(s) 3235
BssECI CCNNGG 5 cut(s) 195, 1256, 1760, 2399, 2644
BssMI GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
BssT1I CCWWGG 3 cut(s) 195, 1760, 2644
Bst4CI ACNGT 8 cut(s) 711, 1202, 1639, 1961, 2010, 2654, 2807, 3336
BstAPI GCANNNNNTGC 2 cut(s) 2300, 3166
BstAUI TGTACA 1 cut(s) 1587
BstC8I GCNNGC 7 cut(s) 326, 1433, 1931, 2947, 3132, 3167, 3237
BstDSI CCRYGG 3 cut(s) 1256, 1760, 2399
BstF5I GGATG 5 cut(s) 1660, 2366, 2409, 2492, 2836
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 7 cut(s) 846, 1096, 1369, 1729, 2020, 3042, 3175
BstMAI GTCTC 6 cut(s) 16, 205, 650, 1110, 1504, 1976
BstMBI GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
BstMWI GCNNNNNNNGC 9 cut(s) 554, 991, 1432, 1702, 1740, 1884, 2300, 2879, 3166
BstNSI RCATGY 2 cut(s) 8, 324
BstSCI CCNGG 2 cut(s) 1388, 1977
BstSFI CTRYAG 3 cut(s) 1201, 2592, 3013
BstSLI GKGCMC 1 cut(s) 1662
BstV1I GCAGC 6 cut(s) 972, 1201, 1732, 2578, 3144, 3180
BstV2I GAAGAC 1 cut(s) 3330
BstX2I RGATCY 1 cut(s) 3039
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 1 cut(s) 3039
BsuI GTATCC 1 cut(s) 2972
BsuRI GGCC 6 cut(s) 222, 525, 909, 1557, 1611, 3165
BtgI CCRYGG 3 cut(s) 1256, 1760, 2399
BtgZI GCGATG 1 cut(s) 1170
BtsCI GGATG 5 cut(s) 1660, 2366, 2409, 2492, 2836
BtsIMutI CAGTG 5 cut(s) 954, 1317, 1347, 2015, 2289
BveI ACCTGC 2 cut(s) 945, 1252
Cac8I GCNNGC 7 cut(s) 326, 1433, 1931, 2947, 3132, 3167, 3237
CaiI CAGNNNCTG 3 cut(s) 1200, 1316, 1858
CciI TCATGA 2 cut(s) 174, 2800
CfoI GCGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 3235
Cfr13I GGNCC 3 cut(s) 524, 1031, 1135
Csp6I GTAC 6 cut(s) 129, 266, 934, 1588, 2650, 2755
CviQI GTAC 6 cut(s) 129, 266, 934, 1588, 2650, 2755
DpnI GATC 7 cut(s) 845, 1095, 1368, 1728, 2019, 3041, 3174
DpnII GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
DraIII CACNNNGTG 1 cut(s) 1478
EaeI YGGCCR 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 397, 2144
Eco130I CCWWGG 3 cut(s) 195, 1760, 2644
Eco147I AGGCCT 2 cut(s) 1611, 3165
Eco24I GRGCYC 2 cut(s) 399, 2146
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 1031, 1135
Eco53kI GAGCTC 2 cut(s) 397, 2144
Eco57I CTGAAG 7 cut(s) 836, 1205, 1422, 2122, 2150, 2265, 3318
EcoICRI GAGCTC 2 cut(s) 397, 2144
EcoO109I RGGNCCY 1 cut(s) 1031
EcoT14I CCWWGG 3 cut(s) 195, 1760, 2644
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 399, 2146
ErhI CCWWGG 3 cut(s) 195, 1760, 2644
FaqI GGGAC 3 cut(s) 196, 1261, 2795
FauI CCCGC 1 cut(s) 1984
FbaI TGATCA 1 cut(s) 843
FblI GTMKAC 3 cut(s) 2201, 2502, 3012
FokI GGATG 5 cut(s) 1667, 2353, 2396, 2499, 2843
FriOI GRGCYC 2 cut(s) 399, 2146
GlaI GCGC 1 cut(s) 547
GsaI CCCAGC 1 cut(s) 2816
GsuI CTGGAG 4 cut(s) 401, 545, 1122, 1626
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 6 cut(s) 222, 525, 909, 1557, 1611, 3165
HapII CCGG 8 cut(s) 450, 741, 813, 1389, 1461, 1979, 2626, 3236
HhaI GCGC 1 cut(s) 548
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 1 cut(s) 3226
HindII GTYRAC 1 cut(s) 3226
HindIII AAGCTT 3 cut(s) 1433, 1927, 2871
HpaII CCGG 8 cut(s) 450, 741, 813, 1389, 1461, 1979, 2626, 3236
Hpy166II GTNNAC 6 cut(s) 1660, 2202, 2403, 2503, 3013, 3226
Hpy8I GTNNAC 6 cut(s) 1660, 2202, 2403, 2503, 3013, 3226
HpyAV CCTTC 8 cut(s) 147, 163, 248, 474, 762, 1622, 2804, 3250
HpyCH4III ACNGT 8 cut(s) 711, 1202, 1639, 1961, 2010, 2654, 2807, 3336
HpyCH4IV ACGT 1 cut(s) 3052
HpyF10VI GCNNNNNNNGC 9 cut(s) 554, 991, 1432, 1702, 1740, 1884, 2300, 2879, 3166
HpySE526I ACGT 1 cut(s) 3052
HspAI GCGC 1 cut(s) 546
KroI GCCGGC 1 cut(s) 3235
KroNI GCCGGC 1 cut(s) 3237
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
LguI GCTCTTC 3 cut(s) 234, 1224, 2215
LmnI GCTCC 7 cut(s) 791, 965, 1702, 1717, 1764, 2527, 2646
Lsp1109I GCAGC 6 cut(s) 972, 1201, 1732, 2578, 3144, 3180
LweI GCATC 4 cut(s) 1069, 2375, 2477, 3180
MaeII ACGT 1 cut(s) 3052
MaeIII GTNAC 5 cut(s) 1196, 1781, 1921, 2284, 2957
MalI GATC 7 cut(s) 845, 1095, 1368, 1728, 2019, 3041, 3174
MbiI CCGCTC 1 cut(s) 794
MboI GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
MfeI CAATTG 1 cut(s) 1054
MflI RGATCY 1 cut(s) 3039
MhlI GDGCHC 3 cut(s) 399, 1662, 2146
MlsI TGGCCA 1 cut(s) 222
MluNI TGGCCA 1 cut(s) 222
MlyI GAGTC 5 cut(s) 114, 801, 1506, 2195, 2576
MmeI TCCRAC 4 cut(s) 534, 730, 1848, 1980
Mox20I TGGCCA 1 cut(s) 222
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 3235
MroXI GAANNNNTTC 3 cut(s) 1146, 1598, 3313
MscI TGGCCA 1 cut(s) 222
MseI TTAA 8 cut(s) 66, 1710, 1997, 2049, 2085, 2261, 3285, 3352
MslI CAYNNNNRTG 6 cut(s) 173, 179, 304, 2181, 2805, 2829
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 8 cut(s) 450, 741, 813, 1389, 1461, 1979, 2626, 3236
MspR9I CCNGG 2 cut(s) 1390, 1979
MunI CAATTG 1 cut(s) 1054
MwoI GCNNNNNNNGC 9 cut(s) 554, 991, 1432, 1702, 1740, 1884, 2300, 2879, 3166
NaeI GCCGGC 1 cut(s) 3237
NciI CCSGG 2 cut(s) 1390, 1979
NcoI CCATGG 1 cut(s) 1760
NdeII GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
NgoMIV GCCGGC 1 cut(s) 3235
NlaIV GGNNCC 3 cut(s) 281, 526, 961
NmuCI GTSAC 2 cut(s) 1921, 2284
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 324
PagI TCATGA 2 cut(s) 174, 2800
PceI AGGCCT 2 cut(s) 1611, 3165
PciSI GCTCTTC 3 cut(s) 234, 1224, 2215
PdiI GCCGGC 1 cut(s) 3237
PdmI GAANNNNTTC 3 cut(s) 1146, 1598, 3313
PflFI GACNNNGTC 1 cut(s) 2807
PflMI CCANNNNNTGG 1 cut(s) 2975
PfoI TCCNGGA 1 cut(s) 1977
PleI GAGTC 5 cut(s) 114, 801, 1506, 2194, 2576
PpsI GAGTC 5 cut(s) 114, 801, 1506, 2194, 2576
PpuMI RGGWCCY 1 cut(s) 1031
Psp124BI GAGCTC 2 cut(s) 399, 2146
Psp5II RGGWCCY 1 cut(s) 1031
PspFI CCCAGC 1 cut(s) 2812
PspN4I GGNNCC 3 cut(s) 281, 526, 961
PspPI GGNCC 3 cut(s) 524, 1031, 1135
PspPPI RGGWCCY 1 cut(s) 1031
PsrI GAACNNNNNNTAC 2 cut(s) 1766, 1798
PstI CTGCAG 1 cut(s) 2596
PstNI CAGNNNCTG 3 cut(s) 1200, 1316, 1858
PsuI RGATCY 1 cut(s) 3039
PsyI GACNNNGTC 1 cut(s) 2807
RsaI GTAC 6 cut(s) 130, 267, 935, 1589, 2651, 2756
RsaNI GTAC 6 cut(s) 129, 266, 934, 1588, 2650, 2755
RseI CAYNNNNRTG 6 cut(s) 173, 179, 304, 2181, 2805, 2829
SacI GAGCTC 2 cut(s) 399, 2146
SapI GCTCTTC 3 cut(s) 234, 1224, 2215
SaqAI TTAA 8 cut(s) 66, 1710, 1997, 2049, 2085, 2261, 3285, 3352
Sau3AI GATC 7 cut(s) 843, 1093, 1366, 1726, 2017, 3039, 3172
Sau96I GGNCC 3 cut(s) 524, 1031, 1135
SchI GAGTC 5 cut(s) 114, 801, 1506, 2195, 2576
ScrFI CCNGG 2 cut(s) 1390, 1979
SduI GDGCHC 3 cut(s) 399, 1662, 2146
SfaNI GCATC 4 cut(s) 1069, 2375, 2477, 3180
SfcI CTRYAG 3 cut(s) 1201, 2592, 3013
SinI GGWCC 2 cut(s) 1031, 1135
SmiMI CAYNNNNRTG 6 cut(s) 173, 179, 304, 2181, 2805, 2829
SmlI CTYRAG 3 cut(s) 50, 1777, 1948
SmoI CTYRAG 3 cut(s) 50, 1777, 1948
SpeI ACTAGT 2 cut(s) 1498, 2069
SseBI AGGCCT 2 cut(s) 1611, 3165
SsiI CCGC 7 cut(s) 329, 794, 797, 1991, 2224, 2537, 3048
SstI GAGCTC 2 cut(s) 399, 2146
StuI AGGCCT 2 cut(s) 1611, 3165
StyD4I CCNGG 2 cut(s) 1388, 1977
StyI CCWWGG 3 cut(s) 195, 1760, 2644
TaaI ACNGT 8 cut(s) 711, 1202, 1639, 1961, 2010, 2654, 2807, 3336
TaiI ACGT 1 cut(s) 3055
TaqI TCGA 8 cut(s) 498, 581, 671, 1077, 1178, 1373, 1504, 2189
TatI WGTACW 3 cut(s) 128, 265, 1587
TauI GCSGC 4 cut(s) 331, 797, 800, 2227
Tru1I TTAA 8 cut(s) 66, 1710, 1997, 2049, 2085, 2261, 3285, 3352
Tru9I TTAA 8 cut(s) 66, 1710, 1997, 2049, 2085, 2261, 3285, 3352
TscAI CASTG 5 cut(s) 961, 1317, 1354, 2015, 2289
TseFI GTSAC 2 cut(s) 1921, 2284
TseI GCWGC 6 cut(s) 985, 1189, 1720, 2591, 3157, 3193
Tsp45I GTSAC 2 cut(s) 1921, 2284
TspGWI ACGGA 2 cut(s) 1245, 2388
TspRI CASTG 5 cut(s) 961, 1317, 1354, 2015, 2289
Tth111I GACNNNGTC 1 cut(s) 2807
Van91I CCANNNNNTGG 1 cut(s) 2975
VneI GTGCAC 1 cut(s) 1658
VpaK11BI GGWCC 2 cut(s) 1031, 1135
XapI RAATTY 6 cut(s) 747, 1306, 1539, 1846, 2155, 2604
XbaI TCTAGA 1 cut(s) 1642
XceI RCATGY 2 cut(s) 8, 324
XcmI CCANNNNNNNNNTGG 3 cut(s) 193, 3077, 3259
XmaJI CCTAGG 1 cut(s) 2644
XmiI GTMKAC 3 cut(s) 2201, 2502, 3012
XmnI GAANNNNTTC 3 cut(s) 1146, 1598, 3313
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.