MD10G1037600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014921
Physical Location & Seq
Forward (+)
4964906 .. 4968894
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1037600.v1.1.491

Sequence Viewer

Length: 3225 bp
ATGGAGCTAAACACAACCCATTTTCAATTTTCCTTCACATTGTTGATTTTATTAACCATGACAAGAATCTCATTAATTACCTCTCTGTCCTCTGATGGCCAAGCCCTTCTCTCCCTCCTCCCTGCAAAGCAATCTTCTGTGCTTTCCTCATGGGACTCATCAAGTGACACACCATGCTCATGGCAAGGCATAACATGCTCTCCCCAAAACAGAGTAATCTCACTCTCTCTCCCCAACATTTTCCTCAACCTCTCCTCTCTCCCTCCGCAGCTCTTCTCCCTCTCGTATCTCCAGCTCCTCAACCTCTCCTCCACCAACATTTCCGGTACCATCCCACCTTTCTTTGGCCAACTCACCCATCTCCGCCTCCTCGACTTATCCTCCAACTCCCTCACCGGCCCCATTCCTCCGGAGCTCGGCCACCTCTCCACCCTCCAATTCCTTTTCCTCAACTCCAACAAACTCTCAGACAAAATGCCTCAACAACTTGCCAACCTCACTTCCCTCCAAGTGCTTTGTCTCCAAGACAACCTCATCAACGGCTCAATACCGTCTCAGTTGGGCTCTCTGGTTTCCCTCCAACAGTTTCGTGTTGGAGGGAATCCATATATAACCGGCGAAATCCCTTCCAAACTAGGCCTGCTCACCAACCTCACAACTTTTGGCGCGGCAGCTACCGCCCTTTCGGGTGTGATTCCATCCACATTTGGGAACTTGATCAACCTCCAAACACTGTCTCTATATGATACTGAGATATCTGGCTCAATACCACCGCAACTCGGGCATTGTTCAGAGCTGAGGAATTTGTATTTGCATATGAACAAGCTTACTGGTTCGATTCCTCCACAATTGGGGAAACTGCAGAAGCTCACCAGCCTGCTTGTTTGGGGCAATGCATTATCAGGACCAATCCCAGCTGAGATTTCAAATTGTTCTTCCCTTGTTGTTCTTGATGCTTCTGCCAATGATCTCTCTGGACCAATCCCAAGAGACTTGGGGAAGCTGGTGGTTCTTGAACAGCTTCATCTCTCCGACAATTTGCTCACTGGAGCAATTCCATCCCAGCTCAGCAACTGCACCAGCCTCACAGCTCTTCAGCTTGACAAGAACCAATTGTCAGGTACAATTCCTTGGCAGGTTGGCAATCTCAAGCTCTTGCAGAGTTTTTTCTTGTGGGGTAATTCTGTATCGGGAACTATACCGGCTTCATTTGGCAACTGCACTGAACTCTATGCACTTGATCTTTCAAGAAACAAGCTTACCGGGTCGATCCCAGAAGAGATTTTTGGATTGAAGAAGCTGAGCAAGCTCTTGCTGTTGGGGAATTCTTTGTCGGGTGGGTTGCCTCGAAGCGTTGCACAATGTCAATCTCTAGTCAGGTTGAGGTTTGGAGAGAACCAGCTTTCGGGACAGATTCCAAAGGAAATAGGCCAGCTGCAAAACCTCGTGTTTCTCGACTTGTACATGAATCATTTCTCCGGAAGATTGCCAACTGAGATTGCCAACATAACAGTTCTTGAACTGTTGGATGTGCACAACAACTATATAAGTGGCGAAATCCCGCCTCAGCTTGGGGAGCTTGTGAATTTGGAGCAGTTTGATCTGAGCAGGAACAGCTTCACTGGAGAAATTCCTTGGAGCTTTGGCAACTTAAGTTACCTGAACAAGCTCATTGCGAACAATAATTTACTCACGGGTTCGATACCAATATCTATCCGGAACCTGCAGAAGCTAACTCTGCTAGATTTGAGCTTCAACAGCCTCTCCGGTCCAATTCCACCCGAAATTGGTCGTGTGACAAGCTTGACAATCAGTTTGGACTTGAGCTGGAATAGTTTTACTGGAGAAATCCCAGAGACAATGGCAGATTTGACGCAATTGCAATCGCTGGACCTTTCGCATAATATGCTCTATGGAAAAATTAAGGTTCTTGGTTCTCTAACTAGTCTCGCGTCCCTAAATATTTCCTGCAACAATTTTTCAGGTGCCATCCCAGTTACGCCATTTTTCAGAACTCTTTCTTCGAATTCATATGTTCAGAATCCACATCTTTGTGAGTCTGTTGATGGGACTACTTGTTCTTCAAGTCTAATGCGGAAGAATGGTTTGAAATCCACAAAAACTGTTGCTTTAATTACTGTGATTCTGGTGTCAGTCACCATAGCACTTGTTGCCTCGTGGGTTCTTATTATGCGAAATCATAGATATATGGTAAAGAAATCTTTAGGAGCAATGGCAGCGTCGTCGGGGGCTGAAGATTTCTCGTATCCATGGACTTTCATCCCATTTCAGAAGCTCAATTTCACCATTGATAACATCTTGGATTGTTTGAAGGAGGAAAATGTGATTGGAAAAGGCTGTTCTGGAATCGTCTATAAGGCCGAATTACACAATGGGGATTTGATTGCGGTGAAAAAGCTATGGAAAACGAAGCAAGATGAAGAGCCAATAGACTCTTTTGCTGCAGAAATTCAAATTCTTGGACACATTCGCCATCGGAACATTGTGAAGCTCCTAGGTTACTGTTCGAATAGAAGTGTCAAGCTGCTTCTTTATAACTACATTCCCAATGGCAATCTGCAGCAGCTATTGCAAGGGAATAGGAACTTGGACTGGGAAACTCGGTACAAGATTGCAATTGGATCAGCTCAAGGTTTAGCATACCTTCATCACGATTGTGTCCCGACGATTCTGCACAGGGATGTCAAGTGCAACAACATACTACTGGATTCAAAGTATGAAGCCTATCTAGCAGATTTTGGTCTGGCAAAGCTGATGAACTCTCCTACTTACCACCACGCAATGTCGAGAGTAGCTGGCTCTTATGGTTATATTGCCCCAGAGTACGGATACACGATGAACATAACGGAGAAGAGTGACGTCTACAGCTATGGTGTGGTTCTGCTGGAGATCTTAAGTGGGCGTAGCGCTGTTCAGCCGCAGATTGGTGACGTGCTACACATTGTTGAATGGGTGAAGAAGAAGATGGGAAGTTTCGAACCAGCTGTATCGATACTAGATACAAAGCTCCAAGGTCTACCAGATCAAATGGTGCAGGAGATGCTGCAAACACTTGGAATAGCAATGTTCTGTGTGAATTCTTCGCCGGCAGAACGACCAACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAGCCTGAAGAGGGGGGAAAAACCTCCCAACCCCTAATAAAGCAACCTTCAAATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1075

Amino Acids

117.36

Weight (kDa)

6.59

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 67 3.8e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 174 9e-09 Leucine-rich repeat region
LRR_8 PF13855 96 - 155 1.2e-06 Leucine rich repeat
LRR_14 PF23598 115 - 219 7e-11 Leucine-rich repeat region
LRR_14 PF23598 182 - 298 3.2e-09 Leucine-rich repeat region
LRR_8 PF13855 386 - 444 1.9e-07 Leucine rich repeat
LRR_14 PF23598 471 - 557 8.9e-07 Leucine-rich repeat region
LRR_14 PF23598 567 - 681 6.9e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 778 - 1048 1.9e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 779 - 1046 2.7e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2556
AatII GACGTC 1 cut(s) 2883
Acc36I ACCTGC 2 cut(s) 1126, 1731
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 2 cut(s) 326, 1985
AccI GTMKAC 2 cut(s) 2883, 3037
AccII CGCG 2 cut(s) 668, 1952
AccIII TCCGGA 3 cut(s) 409, 1478, 1716
AciI CCGC 9 cut(s) 266, 364, 668, 678, 773, 1562, 2095, 2408, 2939
AclWI GGATC 2 cut(s) 1264, 2650
AcoI YGGCCR 3 cut(s) 97, 346, 418
AcsI RAATTY 8 cut(s) 802, 1324, 1585, 1629, 2026, 2469, 2475, 3097
AcuI CTGAAG 3 cut(s) 1079, 2274, 3189
AcyI GRCGYC 1 cut(s) 2880
AfaI GTAC 5 cut(s) 328, 1123, 1463, 2627, 2846
AfeI AGCGCT 1 cut(s) 2929
AflII CTTAAG 2 cut(s) 1651, 2914
AhlI ACTAGT 1 cut(s) 1943
AjiI CACGTC 1 cut(s) 2953
AjuI GAANNNNNNNTTGG 6 cut(s) 1269, 1301, 2331, 2363, 2591, 2623
AleI CACNNNNGTG 1 cut(s) 2676
AloI GAACNNNNNNTCC 2 cut(s) 2062, 2094
Alw21I GWGCWC 2 cut(s) 417, 1536
Alw26I GTCTC 6 cut(s) 524, 558, 741, 984, 1850, 1952
Alw44I GTGCAC 1 cut(s) 1532
AlwI GGATC 2 cut(s) 1264, 2650
AlwNI CAGNNNCTG 1 cut(s) 1074
Ama87I CYCGRG 1 cut(s) 779
Aor13HI TCCGGA 3 cut(s) 409, 1478, 1716
Aor51HI AGCGCT 1 cut(s) 2929
AoxI GGCC 7 cut(s) 97, 346, 397, 418, 637, 1429, 2379
ApaLI GTGCAC 1 cut(s) 1532
ApeKI GCWGC 9 cut(s) 268, 671, 1435, 2237, 2462, 2545, 2581, 2584, 3064
ApoI RAATTY 8 cut(s) 802, 1324, 1585, 1629, 2026, 2469, 2475, 3097
ArsI GACNNNNNNTTYG 2 cut(s) 1798, 1830
AseI ATTAAT 1 cut(s) 74
Asp700I GAANNNNTTC 4 cut(s) 1020, 1472, 1616, 2017
Asp718I GGTACC 1 cut(s) 326
AspA2I CCTAGG 1 cut(s) 2515
AspLEI GCGC 2 cut(s) 668, 2930
AspS9I GGNCC 5 cut(s) 398, 905, 977, 1769, 1891
AsuC2I CCSGG 1 cut(s) 1264
AsuHPI GGTGA 9 cut(s) 346, 385, 637, 862, 2149, 2296, 2422, 2960, 2986
AsuII TTCGAA 3 cut(s) 2024, 2528, 2997
AvaI CYCGRG 1 cut(s) 779
AvaII GGWCC 4 cut(s) 905, 977, 1769, 1891
AvrII CCTAGG 1 cut(s) 2515
BaeGI GKGCMC 1 cut(s) 1536
BalI TGGCCA 2 cut(s) 99, 348
BanI GGYRCC 2 cut(s) 326, 1985
BanII GRGCYC 2 cut(s) 417, 566
BauI CACGAG 2 cut(s) 1445, 2176
Bbv12I GWGCWC 2 cut(s) 417, 1536
BbvCI CCTCAGC 2 cut(s) 797, 1566
BbvI GCAGC 9 cut(s) 280, 683, 1422, 2249, 2449, 2532, 2593, 2596, 3051
BccI CCATC 9 cut(s) 89, 338, 366, 706, 1066, 1997, 2060, 2502, 2980
BceAI ACGGC 1 cut(s) 556
BcgI CGANNNNNNTGC 4 cut(s) 2226, 2260, 2674, 2708
BciVI GTATCC 2 cut(s) 2277, 2843
BclI TGATCA 1 cut(s) 717
BcnI CCSGG 1 cut(s) 1264
BcoDI GTCTC 6 cut(s) 524, 558, 741, 984, 1850, 1952
BcuI ACTAGT 1 cut(s) 1943
BfaI CTAG 7 cut(s) 635, 1373, 1742, 1944, 2516, 2750, 3017
BfmI CTRYAG 5 cut(s) 860, 1724, 2463, 2579, 2884
BfoI RGCGCY 1 cut(s) 2931
BfrI CTTAAG 2 cut(s) 1651, 2914
BfuAI ACCTGC 2 cut(s) 1126, 1731
BfuI GTATCC 2 cut(s) 2277, 2843
BglII AGATCT 1 cut(s) 2910
BlnI CCTAGG 1 cut(s) 2515
BlpI GCTNAGC 2 cut(s) 1067, 1301
Bme1390I CCNGG 1 cut(s) 1264
Bme18I GGWCC 4 cut(s) 905, 977, 1769, 1891
BmeT110I CYCGRG 1 cut(s) 779
BmgBI CACGTC 1 cut(s) 2953
BmgT120I GGNCC 5 cut(s) 398, 905, 977, 1769, 1891
BmiI GGNNCC 4 cut(s) 328, 400, 1721, 1987
BmrFI CCNGG 1 cut(s) 1264
BmrI ACTGGG 2 cut(s) 1988, 2623
BmsI GCATC 2 cut(s) 943, 3051
BmuI ACTGGG 2 cut(s) 1988, 2623
BpmI CTGGAG 5 cut(s) 275, 1068, 1644, 1863, 2927
Bpu10I CCTNAGC 2 cut(s) 797, 1566
Bpu1102I GCTNAGC 2 cut(s) 1067, 1301
Bpu14I TTCGAA 3 cut(s) 2024, 2528, 2997
BpuEI CTTGAG 3 cut(s) 1133, 1843, 2634
BpuMI CCSGG 1 cut(s) 1264
Bsa29I ATCGAT 1 cut(s) 3011
BsaHI GRCGYC 1 cut(s) 2880
BsaJI CCNNGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
BsaWI WCCGGW 5 cut(s) 323, 409, 1478, 1716, 1766
Bse118I RCCGGY 4 cut(s) 395, 614, 1201, 3106
Bse1I ACTGG 7 cut(s) 835, 1051, 1627, 1846, 1994, 2618, 2730
Bse3DI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
BseAI TCCGGA 3 cut(s) 409, 1478, 1716
BseCI ATCGAT 1 cut(s) 3011
BseDI CCNNGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
BseGI GGATG 7 cut(s) 330, 698, 1058, 1534, 1989, 2280, 2707
BseMI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
BseNI ACTGG 7 cut(s) 835, 1051, 1627, 1846, 1994, 2618, 2730
BseRI GAGGAG 5 cut(s) 107, 244, 287, 298, 359
BseSI GKGCMC 1 cut(s) 1536
BseXI GCAGC 9 cut(s) 280, 683, 1422, 2249, 2449, 2532, 2593, 2596, 3051
BseYI CCCAGC 2 cut(s) 913, 1062
BsgI GTGCAG 4 cut(s) 1060, 1204, 2678, 3074
Bsh1236I CGCG 2 cut(s) 668, 1952
BshFI GGCC 7 cut(s) 99, 348, 399, 420, 639, 1431, 2381
BshNI GGYRCC 2 cut(s) 326, 1985
BshVI ATCGAT 1 cut(s) 3011
BsiHKAI GWGCWC 2 cut(s) 417, 1536
BsiHKCI CYCGRG 1 cut(s) 779
BslFI GGGAC 5 cut(s) 167, 1422, 1939, 2083, 2666
BsmAI GTCTC 6 cut(s) 524, 558, 741, 984, 1850, 1952
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 5 cut(s) 167, 1422, 1939, 2083, 2666
BsnI GGCC 7 cut(s) 99, 348, 399, 420, 639, 1431, 2381
BsoBI CYCGRG 1 cut(s) 779
Bsp119I TTCGAA 3 cut(s) 2024, 2528, 2997
Bsp1286I GDGCHC 3 cut(s) 417, 566, 1536
Bsp13I TCCGGA 3 cut(s) 409, 1478, 1716
Bsp1407I TGTACA 1 cut(s) 1461
Bsp143I GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
Bsp1720I GCTNAGC 2 cut(s) 1067, 1301
Bsp19I CCATGG 1 cut(s) 2270
BspACI CCGC 9 cut(s) 266, 364, 668, 678, 773, 1562, 2095, 2408, 2939
BspANI GGCC 7 cut(s) 99, 348, 399, 420, 639, 1431, 2381
BspDI ATCGAT 1 cut(s) 3011
BspEI TCCGGA 3 cut(s) 409, 1478, 1716
BspFNI CGCG 2 cut(s) 668, 1952
BspLI GGNNCC 4 cut(s) 328, 400, 1721, 1987
BspMAI CTGCAG 4 cut(s) 864, 1728, 2467, 2583
BspMI ACCTGC 2 cut(s) 1126, 1731
BspPI GGATC 2 cut(s) 1264, 2650
BspQI GCTCTTC 3 cut(s) 278, 1098, 2436
BspT104I TTCGAA 3 cut(s) 2024, 2528, 2997
BspT107I GGYRCC 2 cut(s) 326, 1985
BspTI CTTAAG 2 cut(s) 1651, 2914
BsrDI GCAATG 6 cut(s) 898, 1671, 2238, 2808, 3090, 3140
BsrFI RCCGGY 4 cut(s) 395, 614, 1201, 3106
BsrGI TGTACA 1 cut(s) 1461
BsrI ACTGG 7 cut(s) 835, 1051, 1627, 1846, 1994, 2618, 2730
BssAI RCCGGY 4 cut(s) 395, 614, 1201, 3106
BssECI CCNNGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
BssMI GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
BssNI GRCGYC 1 cut(s) 2880
BssSI CACGAG 2 cut(s) 1445, 2176
BssT1I CCWWGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
Bst2BI CACGAG 2 cut(s) 1445, 2176
Bst4CI ACNGT 8 cut(s) 552, 585, 735, 1513, 1524, 2125, 2140, 2525
Bst6I CTCTTC 6 cut(s) 278, 1098, 1272, 2436, 2867, 3165
BstACI GRCGYC 1 cut(s) 2880
BstAFI CTTAAG 2 cut(s) 1651, 2914
BstAPI GCANNNNNTGC 5 cut(s) 195, 1906, 2171, 2590, 3061
BstAUI TGTACA 1 cut(s) 1461
BstBI TTCGAA 3 cut(s) 2024, 2528, 2997
BstC8I GCNNGC 7 cut(s) 641, 878, 1307, 1433, 2818, 3108, 3164
BstDSI CCRYGG 1 cut(s) 2270
BstF5I GGATG 7 cut(s) 330, 698, 1058, 1534, 1989, 2280, 2707
BstFNI CGCG 2 cut(s) 668, 1952
BstH2I RGCGCY 1 cut(s) 2931
BstHHI GCGC 2 cut(s) 668, 2930
BstKTI GATC 8 cut(s) 720, 970, 1243, 1272, 1603, 2645, 2913, 3046
BstMAI GTCTC 6 cut(s) 524, 558, 741, 984, 1850, 1952
BstMBI GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 1262
BstSFI CTRYAG 5 cut(s) 860, 1724, 2463, 2579, 2884
BstSLI GKGCMC 1 cut(s) 1536
BstUI CGCG 2 cut(s) 668, 1952
BstV1I GCAGC 9 cut(s) 280, 683, 1422, 2249, 2449, 2532, 2593, 2596, 3051
BstX2I RGATCY 1 cut(s) 2910
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 2910
Bsu15I ATCGAT 1 cut(s) 3011
BsuI GTATCC 2 cut(s) 2277, 2843
BsuRI GGCC 7 cut(s) 99, 348, 399, 420, 639, 1431, 2381
BsuTUI ATCGAT 1 cut(s) 3011
BtgI CCRYGG 1 cut(s) 2270
BtrI CACGTC 1 cut(s) 2953
BtsCI GGATG 7 cut(s) 330, 698, 1058, 1534, 1989, 2280, 2707
BtsIMutI CAGTG 4 cut(s) 731, 1044, 1221, 1620
BveI ACCTGC 2 cut(s) 1126, 1731
Cac8I GCNNGC 7 cut(s) 641, 878, 1307, 1433, 2818, 3108, 3164
CaiI CAGNNNCTG 1 cut(s) 1074
CfoI GCGC 2 cut(s) 668, 2930
Cfr10I RCCGGY 4 cut(s) 395, 614, 1201, 3106
Cfr13I GGNCC 5 cut(s) 398, 905, 977, 1769, 1891
ClaI ATCGAT 1 cut(s) 3011
CseI GACGC 3 cut(s) 1882, 1941, 2229
Csp6I GTAC 5 cut(s) 327, 1122, 1462, 2626, 2845
CviAII CATG 8 cut(s) 58, 150, 174, 180, 195, 1465, 2271, 3124
CviQI GTAC 5 cut(s) 327, 1122, 1462, 2626, 2845
DpnI GATC 8 cut(s) 719, 969, 1242, 1271, 1602, 2644, 2912, 3045
DpnII GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
EaeI YGGCCR 3 cut(s) 97, 346, 418
Eam1104I CTCTTC 6 cut(s) 278, 1098, 1272, 2436, 2867, 3165
EarI CTCTTC 6 cut(s) 278, 1098, 1272, 2436, 2867, 3165
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 415
Eco130I CCWWGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
Eco147I AGGCCT 1 cut(s) 639
Eco24I GRGCYC 2 cut(s) 417, 566
Eco32I GATATC 1 cut(s) 756
Eco47I GGWCC 4 cut(s) 905, 977, 1769, 1891
Eco47III AGCGCT 1 cut(s) 2929
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 3 cut(s) 1079, 2274, 3189
Eco88I CYCGRG 1 cut(s) 779
EcoICRI GAGCTC 1 cut(s) 415
EcoRI GAATTC 3 cut(s) 1324, 2026, 3097
EcoRV GATATC 1 cut(s) 756
EcoT14I CCWWGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 2 cut(s) 417, 566
ErhI CCWWGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 8 cut(s) 61, 153, 177, 183, 198, 1468, 2274, 3127
FaqI GGGAC 5 cut(s) 167, 1422, 1939, 2083, 2666
FatI CATG 8 cut(s) 57, 149, 173, 179, 194, 1464, 2270, 3123
FauI CCCGC 1 cut(s) 1569
FauNDI CATATG 2 cut(s) 816, 2032
FbaI TGATCA 1 cut(s) 717
FblI GTMKAC 2 cut(s) 2883, 3037
FokI GGATG 7 cut(s) 317, 685, 1045, 1541, 1976, 2267, 2714
FriOI GRGCYC 2 cut(s) 417, 566
FspBI CTAG 7 cut(s) 635, 1373, 1742, 1944, 2516, 2750, 3017
GlaI GCGC 2 cut(s) 667, 2929
GsaI CCCAGC 2 cut(s) 917, 1066
GsuI CTGGAG 5 cut(s) 275, 1068, 1644, 1863, 2927
HaeII RGCGCY 1 cut(s) 2931
HaeIII GGCC 7 cut(s) 99, 348, 399, 420, 639, 1431, 2381
HgaI GACGC 3 cut(s) 1882, 1941, 2229
HhaI GCGC 2 cut(s) 668, 2930
Hin1I GRCGYC 1 cut(s) 2880
Hin1II CATG 8 cut(s) 61, 153, 177, 183, 198, 1468, 2274, 3127
Hin6I GCGC 2 cut(s) 666, 2928
HinP1I GCGC 2 cut(s) 666, 2928
HindIII AAGCTT 3 cut(s) 824, 1256, 1801
HphI GGTGA 9 cut(s) 346, 385, 637, 862, 2149, 2296, 2422, 2960, 2986
Hpy166II GTNNAC 3 cut(s) 1534, 2884, 3038
Hpy188I TCNGA 9 cut(s) 94, 469, 793, 1033, 1605, 2012, 2040, 2292, 2499
Hpy8I GTNNAC 3 cut(s) 1534, 2884, 3038
Hpy99I CGWCG 3 cut(s) 2245, 2248, 2689
HpyAV CCTTC 7 cut(s) 43, 116, 636, 2326, 2675, 3121, 3219
HpyCH4III ACNGT 8 cut(s) 552, 585, 735, 1513, 1524, 2125, 2140, 2525
HpyCH4IV ACGT 2 cut(s) 2880, 2952
HpySE526I ACGT 2 cut(s) 2880, 2952
Hsp92I GRCGYC 1 cut(s) 2880
Hsp92II CATG 8 cut(s) 61, 153, 177, 183, 198, 1468, 2274, 3127
HspAI GCGC 2 cut(s) 666, 2928
Kpn2I TCCGGA 3 cut(s) 409, 1478, 1716
KpnI GGTACC 1 cut(s) 330
KroI GCCGGC 1 cut(s) 3106
KroNI GCCGGC 1 cut(s) 3108
Ksp22I TGATCA 1 cut(s) 717
Kzo9I GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
LguI GCTCTTC 3 cut(s) 278, 1098, 2436
Lsp1109I GCAGC 9 cut(s) 280, 683, 1422, 2249, 2449, 2532, 2593, 2596, 3051
LweI GCATC 2 cut(s) 943, 3051
MaeI CTAG 7 cut(s) 635, 1373, 1742, 1944, 2516, 2750, 3017
MaeII ACGT 2 cut(s) 2880, 2952
MaeIII GTNAC 8 cut(s) 164, 1655, 1795, 1996, 2155, 2519, 2876, 2948
MalI GATC 8 cut(s) 719, 969, 1242, 1271, 1602, 2644, 2912, 3045
MboI GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
MfeI CAATTG 4 cut(s) 848, 1112, 1877, 2637
MflI RGATCY 1 cut(s) 2910
MhlI GDGCHC 3 cut(s) 417, 566, 1536
MlsI TGGCCA 2 cut(s) 99, 348
MluNI TGGCCA 2 cut(s) 99, 348
MlyI GAGTC 3 cut(s) 149, 2066, 2447
MmeI TCCRAC 6 cut(s) 408, 480, 574, 604, 1056, 1506
Mox20I TGGCCA 2 cut(s) 99, 348
Mph1103I ATGCAT 1 cut(s) 898
MroI TCCGGA 3 cut(s) 409, 1478, 1716
MroNI GCCGGC 1 cut(s) 3106
MroXI GAANNNNTTC 4 cut(s) 1020, 1472, 1616, 2017
MscI TGGCCA 2 cut(s) 99, 348
MseI TTAA 8 cut(s) 53, 74, 1652, 1923, 2132, 2915, 3156, 3223
MslI CAYNNNNRTG 4 cut(s) 178, 2052, 2676, 2700
Msp20I TGGCCA 2 cut(s) 99, 348
MspA1I CMGCKG 3 cut(s) 917, 1435, 3005
MspCI CTTAAG 2 cut(s) 1651, 2914
MspR9I CCNGG 1 cut(s) 1264
MunI CAATTG 4 cut(s) 848, 1112, 1877, 2637
MvnI CGCG 2 cut(s) 668, 1952
NaeI GCCGGC 1 cut(s) 3108
NciI CCSGG 1 cut(s) 1264
NcoI CCATGG 1 cut(s) 2270
NdeI CATATG 2 cut(s) 816, 2032
NdeII GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
NgoMIV GCCGGC 1 cut(s) 3106
NlaIII CATG 8 cut(s) 61, 153, 177, 183, 198, 1468, 2274, 3127
NlaIV GGNNCC 4 cut(s) 328, 400, 1721, 1987
NmeAIII GCCGAG 1 cut(s) 396
NmuCI GTSAC 5 cut(s) 164, 1795, 2155, 2876, 2948
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 1 cut(s) 198
NspV TTCGAA 3 cut(s) 2024, 2528, 2997
OliI CACNNNNGTG 1 cut(s) 2676
PceI AGGCCT 1 cut(s) 639
PciSI GCTCTTC 3 cut(s) 278, 1098, 2436
PcsI WCGNNNNNNNCGW 1 cut(s) 1452
PdiI GCCGGC 1 cut(s) 3108
PdmI GAANNNNTTC 4 cut(s) 1020, 1472, 1616, 2017
PleI GAGTC 3 cut(s) 149, 2065, 2447
PpsI GAGTC 3 cut(s) 149, 2065, 2447
PshBI ATTAAT 1 cut(s) 74
PsiI TTATAA 1 cut(s) 2556
Psp124BI GAGCTC 1 cut(s) 417
PspFI CCCAGC 2 cut(s) 913, 1062
PspN4I GGNNCC 4 cut(s) 328, 400, 1721, 1987
PspPI GGNCC 5 cut(s) 398, 905, 977, 1769, 1891
PstI CTGCAG 4 cut(s) 864, 1728, 2467, 2583
PstNI CAGNNNCTG 1 cut(s) 1074
PsuI RGATCY 1 cut(s) 2910
PvuII CAGCTG 3 cut(s) 917, 1435, 3005
RsaI GTAC 5 cut(s) 328, 1123, 1463, 2627, 2846
RsaNI GTAC 5 cut(s) 327, 1122, 1462, 2626, 2845
RseI CAYNNNNRTG 4 cut(s) 178, 2052, 2676, 2700
SacI GAGCTC 1 cut(s) 417
SapI GCTCTTC 3 cut(s) 278, 1098, 2436
SaqAI TTAA 8 cut(s) 53, 74, 1652, 1923, 2132, 2915, 3156, 3223
Sau3AI GATC 8 cut(s) 717, 967, 1240, 1269, 1600, 2642, 2910, 3043
Sau96I GGNCC 5 cut(s) 398, 905, 977, 1769, 1891
SchI GAGTC 3 cut(s) 149, 2066, 2447
ScrFI CCNGG 1 cut(s) 1264
SduI GDGCHC 3 cut(s) 417, 566, 1536
SfaNI GCATC 2 cut(s) 943, 3051
SfcI CTRYAG 5 cut(s) 860, 1724, 2463, 2579, 2884
SfuI TTCGAA 3 cut(s) 2024, 2528, 2997
SinI GGWCC 4 cut(s) 905, 977, 1769, 1891
SmiMI CAYNNNNRTG 4 cut(s) 178, 2052, 2676, 2700
SmlI CTYRAG 5 cut(s) 1148, 1651, 1822, 2649, 2914
SmoI CTYRAG 5 cut(s) 1148, 1651, 1822, 2649, 2914
SpeI ACTAGT 1 cut(s) 1943
SseBI AGGCCT 1 cut(s) 639
SsiI CCGC 9 cut(s) 266, 364, 668, 678, 773, 1562, 2095, 2408, 2939
SspI AATATT 1 cut(s) 1963
SspMI CTAG 7 cut(s) 635, 1373, 1742, 1944, 2516, 2750, 3017
SstI GAGCTC 1 cut(s) 417
StuI AGGCCT 1 cut(s) 639
StyD4I CCNGG 1 cut(s) 1262
StyI CCWWGG 5 cut(s) 1130, 1634, 2270, 2515, 3031
TaaI ACNGT 8 cut(s) 552, 585, 735, 1513, 1524, 2125, 2140, 2525
TaiI ACGT 2 cut(s) 2883, 2955
TatI WGTACW 1 cut(s) 1461
TauI GCSGC 2 cut(s) 671, 2941
Tru1I TTAA 8 cut(s) 53, 74, 1652, 1923, 2132, 2915, 3156, 3223
Tru9I TTAA 8 cut(s) 53, 74, 1652, 1923, 2132, 2915, 3156, 3223
TscAI CASTG 4 cut(s) 738, 1051, 1228, 1627
TseFI GTSAC 5 cut(s) 164, 1795, 2155, 2876, 2948
TseI GCWGC 9 cut(s) 268, 671, 1435, 2237, 2462, 2545, 2581, 2584, 3064
Tsp45I GTSAC 5 cut(s) 164, 1795, 2155, 2876, 2948
TspGWI ACGGA 2 cut(s) 2862, 2882
TspRI CASTG 4 cut(s) 738, 1051, 1228, 1627
Vha464I CTTAAG 2 cut(s) 1651, 2914
VneI GTGCAC 1 cut(s) 1532
VpaK11BI GGWCC 4 cut(s) 905, 977, 1769, 1891
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 8 cut(s) 802, 1324, 1585, 1629, 2026, 2469, 2475, 3097
XceI RCATGY 1 cut(s) 198
XcmI CCANNNNNNNNNTGG 1 cut(s) 3130
XmaJI CCTAGG 1 cut(s) 2515
XmiI GTMKAC 2 cut(s) 2883, 3037
XmnI GAANNNNTTC 4 cut(s) 1020, 1472, 1616, 2017
XspI CTAG 7 cut(s) 635, 1373, 1742, 1944, 2516, 2750, 3017
ZraI GACGTC 1 cut(s) 2881
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.