Rroxscaffold_7G00193690
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014921
Physical Location & Seq
Reverse (-)
35203262 .. 35206536
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193690.1

Sequence Viewer

Length: 2523 bp
ATGTCCAAACTAACCTTGGTCTCCTCTCTGTCCCCTGATGGCCAAGCTCTTCTCTCCCTTCTTCCTGCAAAGCAATCTTCCTCTGTACTCTCCTCATGGAACCCATCAAGCCAGACACCATGTTCATGGCAAGGAATCACATGCTCACCACAAAACAGAGTCATCTCCCTCTCTCTCCCCAACGTTTTCCTCAATCTCTCCTCTTTACCCCCACAGCTCTCCTCTCTTTCATCTCTCCAGCTTCTCAATCTCTCCTCCACCAACATTTCAGGCACTATCCCACCCTCCTTTGGACAACTTACTCATCTTCGCCTCCTCGACCTCTCCTCCAACTCTCTGTTTGGTCCGATTCCTCCAGAACTAGGCGCTGTCTCTGCTCTCCAGTTCCTCTTCTTGAACTCAAACAGATTGTCAGATAAAATCCCTCAACAACTTGCCAACCTCACTTCACTTCAAGTCCTTTGTCTCCAAGACAATCTCATCAACGGCTCGATACCCTCCCAACTCGGTTCTTTGGCTTCGCTCCAACAGTTTCGTGTTGGTGGGAATCCATATATAACCGGAGAAATTCCTTCCCAGTTGGGACTACTCACCAATCTCACCACATTCGGAGCCGCGGCTACTGGACTTTCCGGGATTATTCCACCCACATTTGGAAACTTGATCAATCTTCAGACACTGGCGCTCTATGATACTGAAATAGTTGGTTCAATACCACCCGAAATCGGGCTTTGTTCTGAGCTGAGGAACTTGTACTTGCACATGAACAAGCTCACTGGCTCCATTCCTCCACAATTAGGTAAGCTGCAAAAGCTCACCAGCCTGCTTGTTTGGGGAAATGACTTATCAGGATCAATCCCCGCTGAGATTTCCAACTGTTCCTCACTTGTTGTTCTCGATGCTTCTGCAAATGATCTCACTGGAGAGATTCCAAGAGACTTGGGGAAGCTGGTGCTCCTTGAACAGCTTCACCTATCGGATAACTCGCTTACAGGGTCGATTCCATCGCAGCTCAGCAACTGTAGCAGCCTTACAGCTCTCCAGCTCGACAAGAATCAGTTATCAGGGACAATTCCATGGCAGGTTGGTAATCTGAAATACTTGCAGAGTTTCTTCTTGTGGGGCAATGCAGTGTCTGGAACTATTCCTGCCTCGTTTGGGAACTGCACTGAACTGTATGCACTTGATCTTTCGAGAAACAAGCTCACCGGGTCAATACCAGAAGAGATTTTCGGTTTGAAGAAACTGAGCAAGCTTTTGCTTCTTGGAAATTCTTTATCTGGCAGGTTGCCACCTATTGTAGCAAAATGTCAATCTCTAGTGCGGCTGAGACTTGGTGAGAACCAGCTTTCAGGACAAATTCCAAAAGAGATAGGCCAACTGCAAAACCTTGTGTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGCCTTCCCATTGAGATTGCCAACATCACAGTTCTTGAGCTACTGGATGTGCACAATAACTACATTGGAGGCGAAATCCCATCTCAGTTTGGGGAGCTTGTTCTTGGTTTTCTAACTAGTCTCGCTTCCCTTAATATCTCCTGCAATGATTTCTCAGGTGCTATCCCAATAAACACCCCGTTCTTCAGAACTCTCTCCTCAAATTCTTACCTTCAAAATCCACATCTTTGTGAGTCTAATGATGGCTCTACTTGCTCTTCAAGTCTAATGCGGAAGAATGGTTTGAAATCTTCGAAAACTATTGCTTTAATTTCTGTGATTCTCTCTTCAGTAACCATAGCAGTTGTTGCTTCATGGATTCTTGTGATGCGAAATCATAGATATATGGTAGAGAAATCTTCAGGAGCATTAGCATCCTCGTCGGGGGCTGAAGATTTCTCCTATCCGTGGACTTTCATCCCATTCCAGAAGTTCAATTTCACTATTGACAACATCTTGGATTGTTTGAAAGAAGAAAATGTGATTGGAAAAGGATGTTCTGGGGTCGTCTACAAGGCAGAAATGCAAACCGGAGAGTTAATTGCGGTGAAAAAGTTGTGGAAGACAAAGCGAGAGGAAGAACCAATAGACTCTTTCGCTGCAGAGATTCAAATTCTTGGACACATTCGCCACCGGAACATTGTGAAGCTCCTAGGGTACTGTTCAAATAGAAAGTATGGATACACAATGAACATAACAGAGAAGAGTGATGTCTACAGCTATGGTGTGGTTTTGCTAGAGATCCTAAGCGGACGTAATGCCGTTCAGCCTCAGATTGGTGATGGACTACACATTGTTGAATGGGTGAAGAAGAAGATGGGAAGTTTCGAACCAGCTTTATCAGTACTAGATACAAAGCTCCAAGGCCTGCCAGATCAAATGGTTCAAGAGATGCTGCAAACACTAGGAATAGCAATGTTTTGTGTCAACTCTTCGCCTGCAGAACGACCCACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAACCTGAAGAATGGGGAAAAACTTCCCAACCCCTAATAAAGCAGTCTTCAAATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

840

Amino Acids

90.97

Weight (kDa)

6.26

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 11 - 49 1.7e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 67 - 203 8.5e-12 Leucine-rich repeat region
LRR_14 PF23598 218 - 350 3.4e-08 Leucine-rich repeat region
LRR_8 PF13855 368 - 426 1.5e-07 Leucine rich repeat
LRR_14 PF23598 420 - 563 8.7e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 646 - 714 2.7e-09 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 647 - 717 1.7e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34110 AT1G34110
fragaria_vesca FvH4_2g13450
malus_domestica MD05G1035300.v1.1 MD10G1037600.v1.1
prunus_persica Prupe.8G043000_v2.0.a1
pyrus_communis pycom05g02560
rosa_chinensis RchiOBHm_Chr6g0275071
rosa_laevigata RLG00000013500
rosa_multiflora Rmu_sc0000447.1_g000012
rosa_roxburghii Rroxscaffold_7G00193690
rosa_rugosa Rorug06G0087900
rosa_samantha Rh6AG202200 Rh6BG205600 Rh6CG207500 Rh6DG197400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1072, 1275
AccI GTMKAC 2 cut(s) 1977, 2181
AccII CGCG 1 cut(s) 617
AciI CCGC 7 cut(s) 615, 617, 861, 1324, 1699, 2012, 2217
AclI AACGTT 1 cut(s) 183
AclWI GGATC 2 cut(s) 859, 2203
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 5 cut(s) 567, 1270, 1359, 1630, 2079
AcuI CTGAAG 6 cut(s) 656, 1597, 1740, 1812, 1878, 2487
AfaI GTAC 5 cut(s) 87, 755, 1409, 2126, 2313
AhlI ACTAGT 1 cut(s) 1544
AjuI GAANNNNNNNTTGG 2 cut(s) 1935, 1967
Alw21I GWGCWC 2 cut(s) 957, 1482
Alw26I GTCTC 6 cut(s) 25, 376, 470, 930, 1324, 1553
Alw44I GTGCAC 1 cut(s) 1478
AlwI GGATC 2 cut(s) 859, 2203
AlwNI CAGNNNCTG 3 cut(s) 679, 1020, 1136
AoxI GGCC 4 cut(s) 40, 1375, 1429, 2332
ApaLI GTGCAC 1 cut(s) 1478
ApeKI GCWGC 5 cut(s) 805, 1009, 1026, 2066, 2362
ApoI RAATTY 5 cut(s) 567, 1270, 1359, 1630, 2079
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
Asp700I GAANNNNTTC 3 cut(s) 966, 1418, 2482
AspA2I CCTAGG 1 cut(s) 2119
AspLEI GCGC 2 cut(s) 368, 685
AspS9I GGNCC 1 cut(s) 344
AsuC2I CCSGG 2 cut(s) 634, 1210
AsuII TTCGAA 2 cut(s) 1721, 2295
AvaII GGWCC 1 cut(s) 344
AvrII CCTAGG 1 cut(s) 2119
BaeGI GKGCMC 1 cut(s) 1482
BalI TGGCCA 1 cut(s) 42
BbsI GAAGAC 2 cut(s) 2036, 2499
Bbv12I GWGCWC 2 cut(s) 957, 1482
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 5 cut(s) 792, 1021, 1038, 2053, 2349
BccI CCATC 7 cut(s) 32, 112, 1012, 1516, 1664, 2243, 2278
BceAI ACGGC 2 cut(s) 502, 2213
BcgI CGANNNNNNTGC 4 cut(s) 887, 921, 1830, 1864
BciVI GTATCC 1 cut(s) 2141
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 634, 1210
BcoDI GTCTC 6 cut(s) 25, 376, 470, 930, 1324, 1553
BcuI ACTAGT 1 cut(s) 1544
BfaI CTAG 7 cut(s) 362, 1319, 1545, 2120, 2204, 2315, 2372
BfmI CTRYAG 4 cut(s) 1021, 2067, 2182, 2406
BfoI RGCGCY 2 cut(s) 369, 686
BfuAI ACCTGC 2 cut(s) 1072, 1275
BfuI GTATCC 1 cut(s) 2141
BisI GCNGC 8 cut(s) 615, 618, 806, 1010, 1027, 1325, 2067, 2363
BlnI CCTAGG 1 cut(s) 2119
BlpI GCTNAGC 1 cut(s) 1013
BlsI GCNGC 8 cut(s) 616, 619, 807, 1011, 1028, 1326, 2068, 2364
BmcAI AGTACT 1 cut(s) 2313
Bme1390I CCNGG 2 cut(s) 634, 1210
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 3 cut(s) 101, 613, 781
BmrFI CCNGG 2 cut(s) 634, 1210
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 4 cut(s) 889, 1785, 1850, 2349
BmuI ACTGGG 1 cut(s) 571
BpiI GAAGAC 2 cut(s) 2036, 2499
BpmI CTGGAG 6 cut(s) 221, 339, 365, 942, 1025, 1446
Bpu10I CCTNAGC 2 cut(s) 743, 2213
Bpu1102I GCTNAGC 1 cut(s) 1013
Bpu14I TTCGAA 2 cut(s) 1721, 2295
BpuEI CTTGAG 1 cut(s) 1484
BpuMI CCSGG 2 cut(s) 634, 1210
BsaI GGTCTC 1 cut(s) 25
BsaJI CCNNGG 6 cut(s) 15, 615, 1076, 1874, 2119, 2329
BsaWI WCCGGW 3 cut(s) 560, 1997, 2100
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse1I ACTGG 7 cut(s) 382, 577, 628, 684, 781, 925, 1476
Bse3DI GCAATG 4 cut(s) 1132, 1579, 2388, 2438
BseDI CCNNGG 6 cut(s) 15, 615, 1076, 1874, 2119, 2329
BseGI GGATG 4 cut(s) 1480, 1841, 1884, 1967
BseMI GCAATG 4 cut(s) 1132, 1579, 2388, 2438
BseMII CTCAG 9 cut(s) 729, 734, 855, 1027, 1238, 1319, 1526, 1596, 2252
BseNI ACTGG 7 cut(s) 382, 577, 628, 684, 781, 925, 1476
BseRI GAGGAG 8 cut(s) 13, 82, 190, 211, 244, 305, 316, 1615
BseSI GKGCMC 1 cut(s) 1482
BseXI GCAGC 5 cut(s) 792, 1021, 1038, 2053, 2349
BsgI GTGCAG 1 cut(s) 1150
Bsh1236I CGCG 1 cut(s) 617
BshFI GGCC 4 cut(s) 42, 1377, 1431, 2334
BsiHKAI GWGCWC 2 cut(s) 957, 1482
BsiSI CCGG 5 cut(s) 561, 633, 1209, 1998, 2101
BslFI GGGAC 3 cut(s) 16, 597, 1081
BsmAI GTCTC 6 cut(s) 25, 376, 470, 930, 1324, 1553
BsmFI GGGAC 3 cut(s) 16, 597, 1081
BsnI GGCC 4 cut(s) 42, 1377, 1431, 2334
Bso31I GGTCTC 1 cut(s) 25
Bsp119I TTCGAA 2 cut(s) 1721, 2295
Bsp1286I GDGCHC 2 cut(s) 957, 1482
Bsp1407I TGTACA 1 cut(s) 1407
Bsp143I GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
Bsp1720I GCTNAGC 1 cut(s) 1013
Bsp19I CCATGG 1 cut(s) 1076
BspACI CCGC 7 cut(s) 615, 617, 861, 1324, 1699, 2012, 2217
BspANI GGCC 4 cut(s) 42, 1377, 1431, 2334
BspCNI CTCAG 9 cut(s) 730, 735, 856, 1026, 1239, 1320, 1525, 1595, 2251
BspFNI CGCG 1 cut(s) 617
BspLI GGNNCC 3 cut(s) 101, 613, 781
BspMAI CTGCAG 2 cut(s) 2071, 2410
BspMI ACCTGC 2 cut(s) 1072, 1275
BspPI GGATC 2 cut(s) 859, 2203
BspQI GCTCTTC 2 cut(s) 54, 1690
BspT104I TTCGAA 2 cut(s) 1721, 2295
BspTNI GGTCTC 1 cut(s) 25
BsrDI GCAATG 4 cut(s) 1132, 1579, 2388, 2438
BsrGI TGTACA 1 cut(s) 1407
BsrI ACTGG 7 cut(s) 382, 577, 628, 684, 781, 925, 1476
BssECI CCNNGG 6 cut(s) 15, 615, 1076, 1874, 2119, 2329
BssMI GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
BssT1I CCWWGG 4 cut(s) 15, 1076, 2119, 2329
Bst4CI ACNGT 6 cut(s) 531, 878, 1022, 1176, 1459, 2129
Bst6I CTCTTC 7 cut(s) 54, 395, 1218, 1690, 1759, 2165, 2404
BstAPI GCANNNNNTGC 1 cut(s) 1775
BstAUI TGTACA 1 cut(s) 1407
BstBI TTCGAA 2 cut(s) 1721, 2295
BstC8I GCNNGC 4 cut(s) 824, 1253, 2336, 2406
BstDSI CCRYGG 3 cut(s) 615, 1076, 1874
BstF5I GGATG 4 cut(s) 1480, 1841, 1884, 1967
BstFNI CGCG 1 cut(s) 617
BstH2I RGCGCY 2 cut(s) 369, 686
BstHHI GCGC 2 cut(s) 368, 685
BstKTI GATC 6 cut(s) 666, 854, 916, 1189, 2211, 2344
BstMAI GTCTC 6 cut(s) 25, 376, 470, 930, 1324, 1553
BstMBI GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
BstMWI GCNNNNNNNGC 5 cut(s) 374, 811, 1023, 1680, 1775
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 632, 1208
BstSFI CTRYAG 4 cut(s) 1021, 2067, 2182, 2406
BstSLI GKGCMC 1 cut(s) 1482
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 5 cut(s) 792, 1021, 1038, 2053, 2349
BstV2I GAAGAC 2 cut(s) 2036, 2499
BstX2I RGATCY 1 cut(s) 2208
BstXI CCANNNNNNTGG 1 cut(s) 126
BstYI RGATCY 1 cut(s) 2208
BsuI GTATCC 1 cut(s) 2141
BsuRI GGCC 4 cut(s) 42, 1377, 1431, 2334
BtgI CCRYGG 3 cut(s) 615, 1076, 1874
BtgZI GCGATG 1 cut(s) 990
BtsCI GGATG 4 cut(s) 1480, 1841, 1884, 1967
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 5 cut(s) 677, 774, 918, 1137, 1167
BveI ACCTGC 2 cut(s) 1072, 1275
Cac8I GCNNGC 4 cut(s) 824, 1253, 2336, 2406
CaiI CAGNNNCTG 3 cut(s) 679, 1020, 1136
CfoI GCGC 2 cut(s) 368, 685
Cfr13I GGNCC 1 cut(s) 344
Cfr42I CCGCGG 1 cut(s) 618
Csp6I GTAC 5 cut(s) 86, 754, 1408, 2125, 2312
CviAII CATG 9 cut(s) 96, 120, 126, 141, 763, 1077, 1411, 1782, 2422
CviQI GTAC 5 cut(s) 86, 754, 1408, 2125, 2312
DpnI GATC 6 cut(s) 665, 853, 915, 1188, 2210, 2343
DpnII GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 7 cut(s) 54, 395, 1218, 1690, 1759, 2165, 2404
EarI CTCTTC 7 cut(s) 54, 395, 1218, 1690, 1759, 2165, 2404
Eco130I CCWWGG 4 cut(s) 15, 1076, 2119, 2329
Eco147I AGGCCT 2 cut(s) 1431, 2334
Eco31I GGTCTC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 6 cut(s) 656, 1597, 1740, 1812, 1878, 2487
EcoT14I CCWWGG 4 cut(s) 15, 1076, 2119, 2329
ErhI CCWWGG 4 cut(s) 15, 1076, 2119, 2329
FaeI CATG 9 cut(s) 99, 123, 129, 144, 766, 1080, 1414, 1785, 2425
FaqI GGGAC 3 cut(s) 16, 597, 1081
FatI CATG 9 cut(s) 95, 119, 125, 140, 762, 1076, 1410, 1781, 2421
FauI CCCGC 1 cut(s) 868
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 2 cut(s) 1977, 2181
Fnu4HI GCNGC 8 cut(s) 615, 618, 806, 1010, 1027, 1325, 2067, 2363
FokI GGATG 4 cut(s) 1487, 1828, 1871, 1974
Fsp4HI GCNGC 8 cut(s) 615, 618, 806, 1010, 1027, 1325, 2067, 2363
FspBI CTAG 7 cut(s) 362, 1319, 1545, 2120, 2204, 2315, 2372
GlaI GCGC 2 cut(s) 367, 684
GluI GCNGC 8 cut(s) 615, 618, 806, 1010, 1027, 1325, 2067, 2363
GsuI CTGGAG 6 cut(s) 221, 339, 365, 942, 1025, 1446
HaeII RGCGCY 2 cut(s) 369, 686
HaeIII GGCC 4 cut(s) 42, 1377, 1431, 2334
HapII CCGG 5 cut(s) 561, 633, 1209, 1998, 2101
HhaI GCGC 2 cut(s) 368, 685
Hin1II CATG 9 cut(s) 99, 123, 129, 144, 766, 1080, 1414, 1785, 2425
Hin6I GCGC 2 cut(s) 366, 683
HinP1I GCGC 2 cut(s) 366, 683
HincII GTYRAC 1 cut(s) 2395
HindII GTYRAC 1 cut(s) 2395
HindIII AAGCTT 1 cut(s) 1253
HpaII CCGG 5 cut(s) 561, 633, 1209, 1998, 2101
Hpy166II GTNNAC 5 cut(s) 1480, 1878, 1978, 2182, 2395
Hpy188I TCNGA 9 cut(s) 348, 415, 611, 675, 739, 979, 1095, 1616, 2241
Hpy8I GTNNAC 5 cut(s) 1480, 1878, 1978, 2182, 2395
Hpy99I CGWCG 1 cut(s) 1852
HpyAV CCTTC 5 cut(s) 68, 582, 1442, 1649, 2419
HpyCH4III ACNGT 6 cut(s) 531, 878, 1022, 1176, 1459, 2129
HpyCH4IV ACGT 2 cut(s) 183, 2221
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 811, 1023, 1680, 1775
HpySE526I ACGT 2 cut(s) 183, 2221
Hsp92II CATG 9 cut(s) 99, 123, 129, 144, 766, 1080, 1414, 1785, 2425
HspAI GCGC 2 cut(s) 366, 683
Ksp22I TGATCA 1 cut(s) 663
KspI CCGCGG 1 cut(s) 618
Kzo9I GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
LguI GCTCTTC 2 cut(s) 54, 1690
LmnI GCTCC 8 cut(s) 528, 611, 785, 960, 1522, 1832, 2121, 2331
Lsp1109I GCAGC 5 cut(s) 792, 1021, 1038, 2053, 2349
LweI GCATC 4 cut(s) 889, 1785, 1850, 2349
MaeI CTAG 7 cut(s) 362, 1319, 1545, 2120, 2204, 2315, 2372
MaeII ACGT 2 cut(s) 183, 2221
MaeIII GTNAC 1 cut(s) 1759
MalI GATC 6 cut(s) 665, 853, 915, 1188, 2210, 2343
MboI GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
MflI RGATCY 1 cut(s) 2208
MhlI GDGCHC 2 cut(s) 957, 1482
MlsI TGGCCA 1 cut(s) 42
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 3 cut(s) 168, 1670, 2051
MmeI TCCRAC 3 cut(s) 354, 550, 897
Mox20I TGGCCA 1 cut(s) 42
MroXI GAANNNNTTC 3 cut(s) 966, 1418, 2482
MscI TGGCCA 1 cut(s) 42
MseI TTAA 5 cut(s) 1560, 1736, 2006, 2454, 2521
MslI CAYNNNNRTG 2 cut(s) 124, 1656
Msp20I TGGCCA 1 cut(s) 42
MspA1I CMGCKG 2 cut(s) 617, 863
MspI CCGG 5 cut(s) 561, 633, 1209, 1998, 2101
MspR9I CCNGG 2 cut(s) 634, 1210
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 5 cut(s) 374, 811, 1023, 1680, 1775
NciI CCSGG 2 cut(s) 634, 1210
NcoI CCATGG 1 cut(s) 1076
NdeII GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
NlaIII CATG 9 cut(s) 99, 123, 129, 144, 766, 1080, 1414, 1785, 2425
NlaIV GGNNCC 3 cut(s) 101, 613, 781
NspI RCATGY 1 cut(s) 144
NspV TTCGAA 2 cut(s) 1721, 2295
PceI AGGCCT 2 cut(s) 1431, 2334
PciSI GCTCTTC 2 cut(s) 54, 1690
PdmI GAANNNNTTC 3 cut(s) 966, 1418, 2482
PfoI TCCNGGA 1 cut(s) 632
PkrI GCNGC 8 cut(s) 616, 619, 807, 1011, 1028, 1326, 2068, 2364
PleI GAGTC 3 cut(s) 167, 1669, 2051
PpsI GAGTC 3 cut(s) 167, 1669, 2051
Psp1406I AACGTT 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 101, 613, 781
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 2 cut(s) 2071, 2410
PstNI CAGNNNCTG 3 cut(s) 679, 1020, 1136
PsuI RGATCY 1 cut(s) 2208
RsaI GTAC 5 cut(s) 87, 755, 1409, 2126, 2313
RsaNI GTAC 5 cut(s) 86, 754, 1408, 2125, 2312
RseI CAYNNNNRTG 2 cut(s) 124, 1656
SacII CCGCGG 1 cut(s) 618
SapI GCTCTTC 2 cut(s) 54, 1690
SaqAI TTAA 5 cut(s) 1560, 1736, 2006, 2454, 2521
SatI GCNGC 8 cut(s) 615, 618, 806, 1010, 1027, 1325, 2067, 2363
Sau3AI GATC 6 cut(s) 663, 851, 913, 1186, 2208, 2341
Sau96I GGNCC 1 cut(s) 344
ScaI AGTACT 1 cut(s) 2313
SchI GAGTC 3 cut(s) 168, 1670, 2051
ScrFI CCNGG 2 cut(s) 634, 1210
SduI GDGCHC 2 cut(s) 957, 1482
SfaNI GCATC 4 cut(s) 889, 1785, 1850, 2349
SfcI CTRYAG 4 cut(s) 1021, 2067, 2182, 2406
Sfr303I CCGCGG 1 cut(s) 618
SfuI TTCGAA 2 cut(s) 1721, 2295
SgrBI CCGCGG 1 cut(s) 618
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 124, 1656
SmlI CTYRAG 1 cut(s) 1463
SmoI CTYRAG 1 cut(s) 1463
SpeI ACTAGT 1 cut(s) 1544
SseBI AGGCCT 2 cut(s) 1431, 2334
SsiI CCGC 7 cut(s) 615, 617, 861, 1324, 1699, 2012, 2217
SspMI CTAG 7 cut(s) 362, 1319, 1545, 2120, 2204, 2315, 2372
StuI AGGCCT 2 cut(s) 1431, 2334
StyD4I CCNGG 2 cut(s) 632, 1208
StyI CCWWGG 4 cut(s) 15, 1076, 2119, 2329
TaaI ACNGT 6 cut(s) 531, 878, 1022, 1176, 1459, 2129
TaiI ACGT 2 cut(s) 186, 2224
TaqI TCGA 8 cut(s) 318, 491, 897, 998, 1047, 1193, 1721, 2295
TatI WGTACW 4 cut(s) 85, 753, 1407, 2311
TauI GCSGC 3 cut(s) 617, 620, 1327
Tru1I TTAA 5 cut(s) 1560, 1736, 2006, 2454, 2521
Tru9I TTAA 5 cut(s) 1560, 1736, 2006, 2454, 2521
TscAI CASTG 5 cut(s) 684, 781, 925, 1137, 1174
TseI GCWGC 5 cut(s) 805, 1009, 1026, 2066, 2362
TspDTI ATGAA 8 cut(s) 114, 219, 779, 1427, 1770, 1873, 2171, 2438
TspGWI ACGGA 1 cut(s) 1863
TspRI CASTG 5 cut(s) 684, 781, 925, 1137, 1174
VneI GTGCAC 1 cut(s) 1478
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 5 cut(s) 567, 1270, 1359, 1630, 2079
XceI RCATGY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 2 cut(s) 13, 2428
XmaJI CCTAGG 1 cut(s) 2119
XmiI GTMKAC 2 cut(s) 1977, 2181
XmnI GAANNNNTTC 3 cut(s) 966, 1418, 2482
XspI CTAG 7 cut(s) 362, 1319, 1545, 2120, 2204, 2315, 2372
ZrmI AGTACT 1 cut(s) 2313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.